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The map label for this gene is ilvB2 [H]

Identifier: 183221733

GI number: 183221733

Start: 2431654

End: 2433381

Strand: Reverse

Name: ilvB2 [H]

Synonym: LEPBI_I2362

Alternate gene names: 183221733

Gene position: 2433381-2431654 (Counterclockwise)

Preceding gene: 183221735

Following gene: 183221732

Centisome position: 67.6

GC content: 44.73

Gene sequence:

>1728_bases
ATGACAATAGGGGGGAAACGAATGACATCGACAACCGAAGCGATTACTGGCGGCCGGCTTATGGTCGAATTATTGGAAGA
AGCGGGTGTGGAAATCGTCTTTGGATACCCAGGTGGAGCCATCCTCCCTTTCTACGACGAACTCTATCATAGCAAAAAAA
TAAAACACATCCTTGTCCGCCATGAACAAGGTGCCATCCATATGGCAGAAGGGTATGCACGTTCCACAGGTAAGTTAGGT
GTTTGTATTGCCACTTCGGGCCCAGGGGCAACGAATTTGATCACTGGCCTTACGGATGCCAAAATGGATTCTGTTCCCAT
ACTTGCGATCACAGGCCAAGTCGCAACCGATGCCATAGGCACAGACGCCTTCCAAGAAGCCGATATCTTTGGGATCACAA
TCCCCATCACAAAATACAATGCCCTTATCAAAAAAGCAGATGATCTTGCCCGTCATTTTGAAGAAGCCATCAAAATCGCC
ATGGGAGGAAGACCAGGCCCAGTCCTCCTTGATTTTCCAAAAGATGTGCAACTGGGAAAAACAACAGTTCGAAAAGCGCA
AAGCCTAAAAATTGCCCCTCACCATTATGAAAGACCAAAGGTGAAAGGGGACCCACAAGAATTTGCAGATGCTCTGAACG
GAGCCAAACGCCCGTTACTTTATGTCGGTGGGGGAGCGATCAATTCCTTTGCCTCGGCCGAAATCAAAGCCCTTGCAGAA
AAAGCAAATGCACCTGTTACCACAACACTTATGGGTCTTGGTGCCTTTCCTGGAACCCATCCCTTGTCTGTCGGAATGCT
TGGGATGCACGGAACGGCTTATGCCAACAAAGCCGTGTTAGAATGTGATTATATCCTAAACTTAGGTGCTAGGTTTGATG
ACCGCGTTGCCAAATACCAAGACTTTGCGCCAAGTGCTGTGCGGGCCCATATTGACATTGATGCCGCTGAGTTCAACAAA
CGAATCAATGTAGATCATATCTTACATGGGGACTTAAAAGATGCCATTCGTGAAATCCTACCATTTGTGAAAAGTGGAGA
TCGCAGTGAGTGGATTCAAAATATCCAATCACTCAAAAAAAATCATCCACTTGATTTCGATAACAGTGGTGACACAATCA
AACCCCAAGATTTTTTACAAAAGGTATATGCAAAAACCAAGGGAGAGGCCATTGTATCAACCGACGTTGGCCAACACCAA
ATGTGGGCAGCACAGTATTATCTCTTTGATAAACCAAACACTTGGCTTACGTCTGGTGGACTTGGTACGATGGGGTATGG
ACTCCCTGCGGCCATTGGTGCCAAATTTGGAAACCCAGACAAAACTGTGATTTGTGTCACAGGAGATGGTTCTTTCCAAA
TGTGTATCCAAGAACTAGCAACAATCGCTCAATCTAAGTTAGGTGTCAAAATTTTACTGTTTAATAATAACTTTCTTGGA
ATGGTTCGCCAGTGGCAGGAACTCTTTTATGAAGAACGATTCAGTGAATCCCAATGGACTTATAATCCAAACTTTGTAAA
ACTTGCCGAAGCCTATGACATCCCAGCGATGCGGATTGAAAAAAAGTCTGAAATCGAGAAGGGAGTGGAGTTTTTCTTAA
AAGACAGCGGATCAGCCCTCATTGAAGTTATGATCCCTGCGGAAGAAAAAGTATTCCCTATGATCCCTGCCGGAAAATCA
CAACAAGACCTCATAGAATTCAAAGACTTGGGGAAATTGAAAAAATGA

Upstream 100 bases:

>100_bases
CATTTTCCATATAAGGTTCCATTTTTCAGCATTCCTTTTTAGACAGTAAAAATCGACTCAAAAACGTGAGAACATTGACA
ACAGGCTCTTTTTTGCAGAA

Downstream 100 bases:

>100_bases
AACATACACTCAGTATCTTAGTCAATAATCATCCAGGCGTGATGAGCCATGTCTCTGGACTTTTTACAAGACGTGGGTAT
AACATTGATTCGATTGCTGT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTIGGKRMTSTTEAITGGRLMVELLEEAGVEIVFGYPGGAILPFYDELYHSKKIKHILVRHEQGAIHMAEGYARSTGKLG
VCIATSGPGATNLITGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKKADDLARHFEEAIKIA
MGGRPGPVLLDFPKDVQLGKTTVRKAQSLKIAPHHYERPKVKGDPQEFADALNGAKRPLLYVGGGAINSFASAEIKALAE
KANAPVTTTLMGLGAFPGTHPLSVGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKYQDFAPSAVRAHIDIDAAEFNK
RINVDHILHGDLKDAIREILPFVKSGDRSEWIQNIQSLKKNHPLDFDNSGDTIKPQDFLQKVYAKTKGEAIVSTDVGQHQ
MWAAQYYLFDKPNTWLTSGGLGTMGYGLPAAIGAKFGNPDKTVICVTGDGSFQMCIQELATIAQSKLGVKILLFNNNFLG
MVRQWQELFYEERFSESQWTYNPNFVKLAEAYDIPAMRIEKKSEIEKGVEFFLKDSGSALIEVMIPAEEKVFPMIPAGKS
QQDLIEFKDLGKLKK

Sequences:

>Translated_575_residues
MTIGGKRMTSTTEAITGGRLMVELLEEAGVEIVFGYPGGAILPFYDELYHSKKIKHILVRHEQGAIHMAEGYARSTGKLG
VCIATSGPGATNLITGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKKADDLARHFEEAIKIA
MGGRPGPVLLDFPKDVQLGKTTVRKAQSLKIAPHHYERPKVKGDPQEFADALNGAKRPLLYVGGGAINSFASAEIKALAE
KANAPVTTTLMGLGAFPGTHPLSVGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKYQDFAPSAVRAHIDIDAAEFNK
RINVDHILHGDLKDAIREILPFVKSGDRSEWIQNIQSLKKNHPLDFDNSGDTIKPQDFLQKVYAKTKGEAIVSTDVGQHQ
MWAAQYYLFDKPNTWLTSGGLGTMGYGLPAAIGAKFGNPDKTVICVTGDGSFQMCIQELATIAQSKLGVKILLFNNNFLG
MVRQWQELFYEERFSESQWTYNPNFVKLAEAYDIPAMRIEKKSEIEKGVEFFLKDSGSALIEVMIPAEEKVFPMIPAGKS
QQDLIEFKDLGKLKK
>Mature_574_residues
TIGGKRMTSTTEAITGGRLMVELLEEAGVEIVFGYPGGAILPFYDELYHSKKIKHILVRHEQGAIHMAEGYARSTGKLGV
CIATSGPGATNLITGLTDAKMDSVPILAITGQVATDAIGTDAFQEADIFGITIPITKYNALIKKADDLARHFEEAIKIAM
GGRPGPVLLDFPKDVQLGKTTVRKAQSLKIAPHHYERPKVKGDPQEFADALNGAKRPLLYVGGGAINSFASAEIKALAEK
ANAPVTTTLMGLGAFPGTHPLSVGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKYQDFAPSAVRAHIDIDAAEFNKR
INVDHILHGDLKDAIREILPFVKSGDRSEWIQNIQSLKKNHPLDFDNSGDTIKPQDFLQKVYAKTKGEAIVSTDVGQHQM
WAAQYYLFDKPNTWLTSGGLGTMGYGLPAAIGAKFGNPDKTVICVTGDGSFQMCIQELATIAQSKLGVKILLFNNNFLGM
VRQWQELFYEERFSESQWTYNPNFVKLAEAYDIPAMRIEKKSEIEKGVEFFLKDSGSALIEVMIPAEEKVFPMIPAGKSQ
QDLIEFKDLGKLKK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.130890052356, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI21361361, Length=505, Percent_Identity=24.950495049505, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=45.632798573975, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=43.2432432432432, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786717, Length=582, Percent_Identity=32.6460481099656, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=29.0441176470588, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788716, Length=500, Percent_Identity=27, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=510, Percent_Identity=27.0588235294118, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=25.1318101933216, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=25.1318101933216, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=43.2708688245315, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=23.9350912778905, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=563, Percent_Identity=24.6891651865009, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=491, Percent_Identity=23.8289205702648, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=581, Percent_Identity=21.6867469879518, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=27.536231884058, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62888; Mature: 62757

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGGKRMTSTTEAITGGRLMVELLEEAGVEIVFGYPGGAILPFYDELYHSKKIKHILVR
CCCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEE
HEQGAIHMAEGYARSTGKLGVCIATSGPGATNLITGLTDAKMDSVPILAITGQVATDAIG
CCCCCEEEECCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHCC
TDAFQEADIFGITIPITKYNALIKKADDLARHFEEAIKIAMGGRPGPVLLDFPKDVQLGK
CCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCH
TTVRKAQSLKIAPHHYERPKVKGDPQEFADALNGAKRPLLYVGGGAINSFASAEIKALAE
HHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHH
KANAPVTTTLMGLGAFPGTHPLSVGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKYQ
HCCCCEEEHHHHCCCCCCCCCCEEEEEECCCCHHCCCEEEEEHHHHHCCCCCHHHHHHHH
DFAPSAVRAHIDIDAAEFNKRINVDHILHGDLKDAIREILPFVKSGDRSEWIQNIQSLKK
CCCCCCEEEEEECCHHHHCCCCCHHHEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NHPLDFDNSGDTIKPQDFLQKVYAKTKGEAIVSTDVGQHQMWAAQYYLFDKPNTWLTSGG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEEEEEECCCCEEECCC
LGTMGYGLPAAIGAKFGNPDKTVICVTGDGSFQMCIQELATIAQSKLGVKILLFNNNFLG
CCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
MVRQWQELFYEERFSESQWTYNPNFVKLAEAYDIPAMRIEKKSEIEKGVEFFLKDSGSAL
HHHHHHHHHHHHHCCCCCCEECCCEEEEEHHCCCCCCEECCHHHHHHHHHHHHCCCCCEE
IEVMIPAEEKVFPMIPAGKSQQDLIEFKDLGKLKK
EEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIGGKRMTSTTEAITGGRLMVELLEEAGVEIVFGYPGGAILPFYDELYHSKKIKHILVR
CCCCCCCCCHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEEE
HEQGAIHMAEGYARSTGKLGVCIATSGPGATNLITGLTDAKMDSVPILAITGQVATDAIG
CCCCCEEEECCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHCC
TDAFQEADIFGITIPITKYNALIKKADDLARHFEEAIKIAMGGRPGPVLLDFPKDVQLGK
CCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCH
TTVRKAQSLKIAPHHYERPKVKGDPQEFADALNGAKRPLLYVGGGAINSFASAEIKALAE
HHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHH
KANAPVTTTLMGLGAFPGTHPLSVGMLGMHGTAYANKAVLECDYILNLGARFDDRVAKYQ
HCCCCEEEHHHHCCCCCCCCCCEEEEEECCCCHHCCCEEEEEHHHHHCCCCCHHHHHHHH
DFAPSAVRAHIDIDAAEFNKRINVDHILHGDLKDAIREILPFVKSGDRSEWIQNIQSLKK
CCCCCCEEEEEECCHHHHCCCCCHHHEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NHPLDFDNSGDTIKPQDFLQKVYAKTKGEAIVSTDVGQHQMWAAQYYLFDKPNTWLTSGG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEEEEEECCCCEEECCC
LGTMGYGLPAAIGAKFGNPDKTVICVTGDGSFQMCIQELATIAQSKLGVKILLFNNNFLG
CCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
MVRQWQELFYEERFSESQWTYNPNFVKLAEAYDIPAMRIEKKSEIEKGVEFFLKDSGSAL
HHHHHHHHHHHHHCCCCCCEECCCEEEEEHHCCCCCCEECCHHHHHHHHHHHHCCCCCEE
IEVMIPAEEKVFPMIPAGKSQQDLIEFKDLGKLKK
EEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]