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The map label for this gene is hslU [H]

Identifier: 183221725

GI number: 183221725

Start: 2424273

End: 2425691

Strand: Reverse

Name: hslU [H]

Synonym: LEPBI_I2354

Alternate gene names: 183221725

Gene position: 2425691-2424273 (Counterclockwise)

Preceding gene: 183221726

Following gene: 183221722

Centisome position: 67.39

GC content: 41.51

Gene sequence:

>1419_bases
ATGAGTTTCAAAACAATACTTGCAGAAGTCGCAACCCAAGGTGAGGATCTAAACGAACTCACGCCACGCCAAATTGTAGA
GCGTTTGGATGAACACATCATCGGTCAAACCAAAGCCAAACGGGCTGTGGCTGTAGCACTTAGAAATCGTTCGAGACGAA
GAAAGTTAAATGAATCCTTACGAGAAGAAATTTATCCCAAAAACATCATCATGATTGGACCAACGGGAGTTGGAAAAACA
GAAATTGCTCGCCGTTTGTCTAAGTTATGTGGTGCACCTTTTATCAAAGTGGAAGCAACCAAATACACTGAAGTTGGTTA
TGTCGGTCGTGATGTGGAATCCATGGTGCGAGATTTGGCAATGGGTGCTCTCAATTTAGTGAAGTCGGAATTTCGCGATC
GAGTGAAGGATAAGGCAGAAGAGAGAGCAGAAGAGATCATTCTCGATGCCATCCTTCCCCCAATCTTTCACAAAAAAGAA
GAAGATCTAAACCCAGAAGAAAAAGAAAGGTTCCAAAGTTACAAAGAATCCAGAGAGAAGTTCAGAGAAAAACTAAGAAA
AGGAATCCTTTCCGAACAAGAAATTGAAATTGATATCCCAAAACCTTCGGCTCAAACGGGAATGCCTATGTTGCAGGTGT
TTGGTGCTGGAAATATGGAGGAGATGGACAACCAAATCCAAAACCTTCTTGGGGATCTCATGCCAAAAAAATCTGGCAAA
CGAAAGGTGAAAGTCTCTGATGCAGAAAAAATATTACTCGAAGCAGAGGCAGAAAAACTCATCGATTCTGATAAAATCCA
ATCGGAAGCAGTACGCCGAGTGGAAGAGATGGGGATTATTTTTCTCGATGAGATTGATAAAATTGCCGGGAGAGAAGGGC
GCCAAGGGGCAGATGTTTCTAGAGAAGGGGTACAACGTGATTTACTTCCCATCGTGGAAGGATCCACTGTGAATACAAAA
CTGGGTCCCATCAAAACCGATCATATCCTTTTCATTGCGGCTGGTGCCTTTCACATGACAAAACCTTCTGACCTCATCCC
TGAATTACAAGGAAGGTTTCCCATTCGTGTGGAATTGGAAACACTAACAGAGGCAGACTTCATTCAAATTTTAACCACTC
CTAAATCCTCTCTCACCAAACAATACGAAGCCTTACTAGCCACTGAAGGTGTGAAGATTGAATACACAAAAGATGGGATT
GCCGAAATTGCAAAACTTGCCTTCCAAATGAATGAAAAAAATGAAAACATTGGAGCAAGAAGGCTCAATACCATCATGGA
AAAATTATTGGAAGACACAAGTTTTGATGCACCTGATTTGCCAGATGATCAAAAACATGTGGTGATCAATCAGGACTATG
TTTCAGGTAAACTAAAAGGGATCATTGAGGACAAAGACTTAAGTCGATTCATTCTGTAA

Upstream 100 bases:

>100_bases
ACAGATTTAGATCCAAAAGAAATCATAACAAAGGCAATGAACATTACGGCTGACATTTGTATTTATACAAATCATAATTT
GGTGATAGAGGAATTATAAG

Downstream 100 bases:

>100_bases
CAAGAGTTACCCGTTCGAATCCTCGAACGGAGTTTCCAACGAGAACAATGGATGCATGATTTAGATCATCCATTGTTGTG
ATTTTTTCCTTGATCCAATT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSFKTILAEVATQGEDLNELTPRQIVERLDEHIIGQTKAKRAVAVALRNRSRRRKLNESLREEIYPKNIIMIGPTGVGKT
EIARRLSKLCGAPFIKVEATKYTEVGYVGRDVESMVRDLAMGALNLVKSEFRDRVKDKAEERAEEIILDAILPPIFHKKE
EDLNPEEKERFQSYKESREKFREKLRKGILSEQEIEIDIPKPSAQTGMPMLQVFGAGNMEEMDNQIQNLLGDLMPKKSGK
RKVKVSDAEKILLEAEAEKLIDSDKIQSEAVRRVEEMGIIFLDEIDKIAGREGRQGADVSREGVQRDLLPIVEGSTVNTK
LGPIKTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVELETLTEADFIQILTTPKSSLTKQYEALLATEGVKIEYTKDGI
AEIAKLAFQMNEKNENIGARRLNTIMEKLLEDTSFDAPDLPDDQKHVVINQDYVSGKLKGIIEDKDLSRFIL

Sequences:

>Translated_472_residues
MSFKTILAEVATQGEDLNELTPRQIVERLDEHIIGQTKAKRAVAVALRNRSRRRKLNESLREEIYPKNIIMIGPTGVGKT
EIARRLSKLCGAPFIKVEATKYTEVGYVGRDVESMVRDLAMGALNLVKSEFRDRVKDKAEERAEEIILDAILPPIFHKKE
EDLNPEEKERFQSYKESREKFREKLRKGILSEQEIEIDIPKPSAQTGMPMLQVFGAGNMEEMDNQIQNLLGDLMPKKSGK
RKVKVSDAEKILLEAEAEKLIDSDKIQSEAVRRVEEMGIIFLDEIDKIAGREGRQGADVSREGVQRDLLPIVEGSTVNTK
LGPIKTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVELETLTEADFIQILTTPKSSLTKQYEALLATEGVKIEYTKDGI
AEIAKLAFQMNEKNENIGARRLNTIMEKLLEDTSFDAPDLPDDQKHVVINQDYVSGKLKGIIEDKDLSRFIL
>Mature_471_residues
SFKTILAEVATQGEDLNELTPRQIVERLDEHIIGQTKAKRAVAVALRNRSRRRKLNESLREEIYPKNIIMIGPTGVGKTE
IARRLSKLCGAPFIKVEATKYTEVGYVGRDVESMVRDLAMGALNLVKSEFRDRVKDKAEERAEEIILDAILPPIFHKKEE
DLNPEEKERFQSYKESREKFREKLRKGILSEQEIEIDIPKPSAQTGMPMLQVFGAGNMEEMDNQIQNLLGDLMPKKSGKR
KVKVSDAEKILLEAEAEKLIDSDKIQSEAVRRVEEMGIIFLDEIDKIAGREGRQGADVSREGVQRDLLPIVEGSTVNTKL
GPIKTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVELETLTEADFIQILTTPKSSLTKQYEALLATEGVKIEYTKDGIA
EIAKLAFQMNEKNENIGARRLNTIMEKLLEDTSFDAPDLPDDQKHVVINQDYVSGKLKGIIEDKDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=113, Percent_Identity=37.1681415929204, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1790366, Length=457, Percent_Identity=55.1422319474836, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1786642, Length=239, Percent_Identity=33.4728033472803, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=108, Percent_Identity=37.962962962963, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648291, Length=247, Percent_Identity=31.9838056680162, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648289, Length=247, Percent_Identity=31.9838056680162, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR011704
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07724 AAA_2; PF07728 AAA_5; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 53336; Mature: 53205

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKTILAEVATQGEDLNELTPRQIVERLDEHIIGQTKAKRAVAVALRNRSRRRKLNESL
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
REEIYPKNIIMIGPTGVGKTEIARRLSKLCGAPFIKVEATKYTEVGYVGRDVESMVRDLA
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCHHHHHHHHH
MGALNLVKSEFRDRVKDKAEERAEEIILDAILPPIFHKKEEDLNPEEKERFQSYKESREK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
FREKLRKGILSEQEIEIDIPKPSAQTGMPMLQVFGAGNMEEMDNQIQNLLGDLMPKKSGK
HHHHHHHCCCCCCCEEEECCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
RKVKVSDAEKILLEAEAEKLIDSDKIQSEAVRRVEEMGIIFLDEIDKIAGREGRQGADVS
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
REGVQRDLLPIVEGSTVNTKLGPIKTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVELE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHH
TLTEADFIQILTTPKSSLTKQYEALLATEGVKIEYTKDGIAEIAKLAFQMNEKNENIGAR
HHCCCHHHHEEECCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHH
RLNTIMEKLLEDTSFDAPDLPDDQKHVVINQDYVSGKLKGIIEDKDLSRFIL
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHCCHHHEEECCCCHHHHCC
>Mature Secondary Structure 
SFKTILAEVATQGEDLNELTPRQIVERLDEHIIGQTKAKRAVAVALRNRSRRRKLNESL
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
REEIYPKNIIMIGPTGVGKTEIARRLSKLCGAPFIKVEATKYTEVGYVGRDVESMVRDLA
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCHHHHHHHHH
MGALNLVKSEFRDRVKDKAEERAEEIILDAILPPIFHKKEEDLNPEEKERFQSYKESREK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
FREKLRKGILSEQEIEIDIPKPSAQTGMPMLQVFGAGNMEEMDNQIQNLLGDLMPKKSGK
HHHHHHHCCCCCCCEEEECCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
RKVKVSDAEKILLEAEAEKLIDSDKIQSEAVRRVEEMGIIFLDEIDKIAGREGRQGADVS
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
REGVQRDLLPIVEGSTVNTKLGPIKTDHILFIAAGAFHMTKPSDLIPELQGRFPIRVELE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHH
TLTEADFIQILTTPKSSLTKQYEALLATEGVKIEYTKDGIAEIAKLAFQMNEKNENIGAR
HHCCCHHHHEEECCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHH
RLNTIMEKLLEDTSFDAPDLPDDQKHVVINQDYVSGKLKGIIEDKDLSRFIL
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHCCHHHEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA