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The map label for this gene is tgt

Identifier: 183221702

GI number: 183221702

Start: 2400479

End: 2401594

Strand: Reverse

Name: tgt

Synonym: LEPBI_I2329

Alternate gene names: 183221702

Gene position: 2401594-2400479 (Counterclockwise)

Preceding gene: 183221703

Following gene: 183221701

Centisome position: 66.72

GC content: 41.94

Gene sequence:

>1116_bases
GTGTCTTCTATTTTTAAAGAAGGTATTTACGATACAGGTTCTTATGCGAGGACTGGCACTTTAGATCTGAATGGCATCCA
AATCCAAACTCCGATCTTTATGCCAGTGGGCACTCGTGGTAGCATTAAGTCTCTCACTTCTGAAGATATCGATGAACTGG
GTTACAACCTCATCCTTGCCAATACCTACCATCTCTATCTAAAACCAGGCAAAGAAGTCTTCGATCATTTTCAGGGCTTA
AAAAATTTTATGTCCTACAAAAAGGCATTACTCACCGATTCGGGTGGGTTCCAGGTCTTTAGCCTGGCAAGCCTCTTTAA
ATTTGAAGACGATGGGGTTCGTTTCCAATCGCATATTGATGGAAGCCATCATAAATTTACACCTGCCTCTGTGATCGATA
TGCAAAGGTCCATCGGGTCTGACATCATGATGGTACTAGATGATTGTGCCCCTTATGGGAGTGATACCAAACGATTGGAA
CTTGCCTTAGATCGGACGCACCGTTGGGCTAAGGAATCATTTGCGTATTGGATGGAAAATCCAGGTGGGCAAAATGTATT
TCCGATTGTGCAAGGTGGAGTGAATGAAACCCTTCGTCTTCGGAGTTTGGAAACATTACAAAAAATTGATTTTCCAGGAA
TTGCCATTGGGGGCTTAAGTGTGGGCGAACCAAGGCCTGAATACATTCGCATTTTAGAATGTATGGCCCCTCATTTGGAC
AAATCGAGACCGAGATACCTTATGGGAGTGGGAACGGTCGTCGATATCCTGGAAGGTGTGAAAAACGGAATCGATATGTT
CGATTGTGTTTTGCCCACAAGGAATGCTCGGAATGGCCAAGTTTTCACATCAAAAGGCAAGATCAATCTGCGAAATGAAT
CCCATCGCCTGAGTGATTCACCAATTGACCCACAATGTGAATGTAAGGTATGCAAAACATATAGTCTCGGTTACATTCGC
CACTTACATAAAGTAAAGGAATTAACAGCCTTTTCCTTATCTACCTACCATAATTTATTTTTTATGCAGAGCTTCATGGA
AAAGATGCGAAAGTCCATAGAAATAGGCAATTTTCAGGGTTTTTATGACCATTGGAAAAATTTGTTTGGTAGCTAA

Upstream 100 bases:

>100_bases
AAGCCGCTTCGGAAAATGGTTTTACCATCACAGGCCATAGTTTGAATATTTTTGGCAATTGTACAAACTTTGCAACTTGC
GAACATAAAAACAAAAAATA

Downstream 100 bases:

>100_bases
AATTATTAGGTTGACGTACCCATTTTTTTTGAATGCAATTGTACCGTCATTTCTACATTGATTGTAGCTTCATTGAGGAG
TCTCTAGACACACATGGAAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSSIFKEGIYDTGSYARTGTLDLNGIQIQTPIFMPVGTRGSIKSLTSEDIDELGYNLILANTYHLYLKPGKEVFDHFQGL
KNFMSYKKALLTDSGGFQVFSLASLFKFEDDGVRFQSHIDGSHHKFTPASVIDMQRSIGSDIMMVLDDCAPYGSDTKRLE
LALDRTHRWAKESFAYWMENPGGQNVFPIVQGGVNETLRLRSLETLQKIDFPGIAIGGLSVGEPRPEYIRILECMAPHLD
KSRPRYLMGVGTVVDILEGVKNGIDMFDCVLPTRNARNGQVFTSKGKINLRNESHRLSDSPIDPQCECKVCKTYSLGYIR
HLHKVKELTAFSLSTYHNLFFMQSFMEKMRKSIEIGNFQGFYDHWKNLFGS

Sequences:

>Translated_371_residues
MSSIFKEGIYDTGSYARTGTLDLNGIQIQTPIFMPVGTRGSIKSLTSEDIDELGYNLILANTYHLYLKPGKEVFDHFQGL
KNFMSYKKALLTDSGGFQVFSLASLFKFEDDGVRFQSHIDGSHHKFTPASVIDMQRSIGSDIMMVLDDCAPYGSDTKRLE
LALDRTHRWAKESFAYWMENPGGQNVFPIVQGGVNETLRLRSLETLQKIDFPGIAIGGLSVGEPRPEYIRILECMAPHLD
KSRPRYLMGVGTVVDILEGVKNGIDMFDCVLPTRNARNGQVFTSKGKINLRNESHRLSDSPIDPQCECKVCKTYSLGYIR
HLHKVKELTAFSLSTYHNLFFMQSFMEKMRKSIEIGNFQGFYDHWKNLFGS
>Mature_370_residues
SSIFKEGIYDTGSYARTGTLDLNGIQIQTPIFMPVGTRGSIKSLTSEDIDELGYNLILANTYHLYLKPGKEVFDHFQGLK
NFMSYKKALLTDSGGFQVFSLASLFKFEDDGVRFQSHIDGSHHKFTPASVIDMQRSIGSDIMMVLDDCAPYGSDTKRLEL
ALDRTHRWAKESFAYWMENPGGQNVFPIVQGGVNETLRLRSLETLQKIDFPGIAIGGLSVGEPRPEYIRILECMAPHLDK
SRPRYLMGVGTVVDILEGVKNGIDMFDCVLPTRNARNGQVFTSKGKINLRNESHRLSDSPIDPQCECKVCKTYSLGYIRH
LHKVKELTAFSLSTYHNLFFMQSFMEKMRKSIEIGNFQGFYDHWKNLFGS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=348, Percent_Identity=41.0919540229885, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1786607, Length=350, Percent_Identity=42.8571428571429, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=35.933147632312, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI19920510, Length=348, Percent_Identity=40.2298850574713, Blast_Score=277, Evalue=7e-75,
Organism=Drosophila melanogaster, GI21355311, Length=221, Percent_Identity=26.2443438914027, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_LEPBA (B0SCA8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963333.1
- ProteinModelPortal:   B0SCA8
- SMR:   B0SCA8
- GeneID:   6388269
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2262
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   LBIF355278:LBF_2262-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41981; Mature: 41849

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIFKEGIYDTGSYARTGTLDLNGIQIQTPIFMPVGTRGSIKSLTSEDIDELGYNLILA
CCCHHHHCCCCCCCCCEECEEECCCEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEE
NTYHLYLKPGKEVFDHFQGLKNFMSYKKALLTDSGGFQVFSLASLFKFEDDGVRFQSHID
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCC
GSHHKFTPASVIDMQRSIGSDIMMVLDDCAPYGSDTKRLELALDRTHRWAKESFAYWMEN
CCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCC
PGGQNVFPIVQGGVNETLRLRSLETLQKIDFPGIAIGGLSVGEPRPEYIRILECMAPHLD
CCCCEECCEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCC
KSRPRYLMGVGTVVDILEGVKNGIDMFDCVLPTRNARNGQVFTSKGKINLRNESHRLSDS
CCCCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCCCC
PIDPQCECKVCKTYSLGYIRHLHKVKELTAFSLSTYHNLFFMQSFMEKMRKSIEIGNFQG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FYDHWKNLFGS
HHHHHHHHHCC
>Mature Secondary Structure 
SSIFKEGIYDTGSYARTGTLDLNGIQIQTPIFMPVGTRGSIKSLTSEDIDELGYNLILA
CCHHHHCCCCCCCCCEECEEECCCEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEE
NTYHLYLKPGKEVFDHFQGLKNFMSYKKALLTDSGGFQVFSLASLFKFEDDGVRFQSHID
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCC
GSHHKFTPASVIDMQRSIGSDIMMVLDDCAPYGSDTKRLELALDRTHRWAKESFAYWMEN
CCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCC
PGGQNVFPIVQGGVNETLRLRSLETLQKIDFPGIAIGGLSVGEPRPEYIRILECMAPHLD
CCCCEECCEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCC
KSRPRYLMGVGTVVDILEGVKNGIDMFDCVLPTRNARNGQVFTSKGKINLRNESHRLSDS
CCCCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCCCC
PIDPQCECKVCKTYSLGYIRHLHKVKELTAFSLSTYHNLFFMQSFMEKMRKSIEIGNFQG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FYDHWKNLFGS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA