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The map label for this gene is vapI [H]

Identifier: 183221632

GI number: 183221632

Start: 2329457

End: 2329762

Strand: Direct

Name: vapI [H]

Synonym: LEPBI_I2256

Alternate gene names: 183221632

Gene position: 2329457-2329762 (Clockwise)

Preceding gene: 183221631

Following gene: 183221633

Centisome position: 64.71

GC content: 32.35

Gene sequence:

>306_bases
ATGAATAAAGAACTTATGAACATTCATCCTGGCGAAATTCTCTTAGAAGACTTTCTTAAACCTATGGAATTATCCGCTTA
TAAACTTGCACAAAGTACTCTTATTGATCAAAAAAGAATTAGTGAAATTATTCATGGAAAAAGAGCAATTACTGCTGATA
CGGCTCTGAGATTCTCTAAATTCTTCGGAAATTCTCCTGAGTTCTGGCTCTCTATTCAAGCTCATTATGATCTAGAAATC
AAGCAATTTGAATTGAAAAACGAGTTAAGAGCAATTAAGAAATACAAAGAACTCAAAGCCAGTTAA

Upstream 100 bases:

>100_bases
ATTCAATAAGTATCAATATGAAATATAGAATCTGTTTTCATTGGAATAACGGTTCTGTCGAAAATGTTGAAATTGTAGAT
TATCATTAAGGAGATTCTGT

Downstream 100 bases:

>100_bases
ATCCAAGCACGTCGCATAACAGCGACTTACCGCTACGCTTCGGGACAAGCCCTCGCTCGGCCTTCGGCAAATTGTCCTCC
TGGCATTCGCCTTGCGTTCG

Product: putative plasmid maintenance system antidote-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 101; Mature: 101

Protein sequence:

>101_residues
MNKELMNIHPGEILLEDFLKPMELSAYKLAQSTLIDQKRISEIIHGKRAITADTALRFSKFFGNSPEFWLSIQAHYDLEI
KQFELKNELRAIKKYKELKAS

Sequences:

>Translated_101_residues
MNKELMNIHPGEILLEDFLKPMELSAYKLAQSTLIDQKRISEIIHGKRAITADTALRFSKFFGNSPEFWLSIQAHYDLEI
KQFELKNELRAIKKYKELKAS
>Mature_101_residues
MNKELMNIHPGEILLEDFLKPMELSAYKLAQSTLIDQKRISEIIHGKRAITADTALRFSKFFGNSPEFWLSIQAHYDLEI
KQFELKNELRAIKKYKELKAS

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=82, Percent_Identity=34.1463414634146, Blast_Score=62, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11812; Mature: 11812

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELMNIHPGEILLEDFLKPMELSAYKLAQSTLIDQKRISEIIHGKRAITADTALRFSK
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FFGNSPEFWLSIQAHYDLEIKQFELKNELRAIKKYKELKAS
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKELMNIHPGEILLEDFLKPMELSAYKLAQSTLIDQKRISEIIHGKRAITADTALRFSK
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FFGNSPEFWLSIQAHYDLEIKQFELKNELRAIKKYKELKAS
HHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]