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The map label for this gene is algU [H]

Identifier: 183221532

GI number: 183221532

Start: 2238925

End: 2239503

Strand: Reverse

Name: algU [H]

Synonym: LEPBI_I2150

Alternate gene names: 183221532

Gene position: 2239503-2238925 (Counterclockwise)

Preceding gene: 183221533

Following gene: 183221531

Centisome position: 62.21

GC content: 42.66

Gene sequence:

>579_bases
ATGACCCTGCGTGAGAAGGAAAAAATCCTCCTCCAAAAAATCAAAGCAGGGGATCCGACTGCCTACATGACACTTGTCTC
TCCGTTCCGAGAGAGACTTTTCCGAAAAGCAGTTTCCATGGTCAAAGATGGCGATGACGCCGAAGACATTGTCCAAGATG
CTCTCATTTCTGGTTACAAATCCATTCAAAATTTCCGTGCCGAAGCGGGTGTTTACACCTGGCTCTACCGCATTGTGGTG
AATAAGTCCAAGGATTTACTCGCCAAAAAAAAACGGGGTCGCGAAAAACCCATGGATGACTCAGGGGATAACCAATTTGT
GGATTCTCGTGTCGGATATGAAAAAAAATTGGAACTTTCTGAGGAATCCAGTTATCTAATGAGTAAGATTGCTCTCTTAG
AAGATTCCTACAAACAAGTGCTCGAACTTCGTTATTTTGAAAATCTTTCTTATAACGAAATTGCCGAGATCATGGAATGT
AATGTAGGGACTGTGAAGAGCCGTCTCTTTAAGGCGAAGGAGTTTTTAAAGCACCTCATCCAGAAAGATGAAAAGGGAGA
GGGGTTCTTTGAAAAATAG

Upstream 100 bases:

>100_bases
CCATAGAATCTAAACTGCCAGCAGATACCGAAAAACATTGATTGATTTATCGGTTTCGAAATTCTAGGTTCTGATGTCAA
GACCCTTAGAAGGCAAAAAC

Downstream 100 bases:

>100_bases
GTTTATGAATTTAAAAGATTGGTTTCGTGCACAATACCCAGGTCTCTTCCCAGAAGACACTGATTCCGTGGTCCTTGAGA
ACAAAATTTGTTCCTTTTTC

Product: putative RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTLREKEKILLQKIKAGDPTAYMTLVSPFRERLFRKAVSMVKDGDDAEDIVQDALISGYKSIQNFRAEAGVYTWLYRIVV
NKSKDLLAKKKRGREKPMDDSGDNQFVDSRVGYEKKLELSEESSYLMSKIALLEDSYKQVLELRYFENLSYNEIAEIMEC
NVGTVKSRLFKAKEFLKHLIQKDEKGEGFFEK

Sequences:

>Translated_192_residues
MTLREKEKILLQKIKAGDPTAYMTLVSPFRERLFRKAVSMVKDGDDAEDIVQDALISGYKSIQNFRAEAGVYTWLYRIVV
NKSKDLLAKKKRGREKPMDDSGDNQFVDSRVGYEKKLELSEESSYLMSKIALLEDSYKQVLELRYFENLSYNEIAEIMEC
NVGTVKSRLFKAKEFLKHLIQKDEKGEGFFEK
>Mature_191_residues
TLREKEKILLQKIKAGDPTAYMTLVSPFRERLFRKAVSMVKDGDDAEDIVQDALISGYKSIQNFRAEAGVYTWLYRIVVN
KSKDLLAKKKRGREKPMDDSGDNQFVDSRVGYEKKLELSEESSYLMSKIALLEDSYKQVLELRYFENLSYNEIAEIMECN
VGTVKSRLFKAKEFLKHLIQKDEKGEGFFEK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=193, Percent_Identity=32.1243523316062, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22304; Mature: 22173

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLREKEKILLQKIKAGDPTAYMTLVSPFRERLFRKAVSMVKDGDDAEDIVQDALISGYK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SIQNFRAEAGVYTWLYRIVVNKSKDLLAKKKRGREKPMDDSGDNQFVDSRVGYEKKLELS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHCCC
EESSYLMSKIALLEDSYKQVLELRYFENLSYNEIAEIMECNVGTVKSRLFKAKEFLKHLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QKDEKGEGFFEK
CCCCCCCCCCCC
>Mature Secondary Structure 
TLREKEKILLQKIKAGDPTAYMTLVSPFRERLFRKAVSMVKDGDDAEDIVQDALISGYK
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SIQNFRAEAGVYTWLYRIVVNKSKDLLAKKKRGREKPMDDSGDNQFVDSRVGYEKKLELS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHCCC
EESSYLMSKIALLEDSYKQVLELRYFENLSYNEIAEIMECNVGTVKSRLFKAKEFLKHLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QKDEKGEGFFEK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]