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The map label for this gene is tpiA

Identifier: 183221464

GI number: 183221464

Start: 2177053

End: 2177802

Strand: Direct

Name: tpiA

Synonym: LEPBI_I2082

Alternate gene names: 183221464

Gene position: 2177053-2177802 (Clockwise)

Preceding gene: 183221463

Following gene: 183221465

Centisome position: 60.48

GC content: 45.47

Gene sequence:

>750_bases
ATGAGAAAGAAGATCATCGCAGGGAATTGGAAAATGAACCTCACGCTAGCGGAGGCAAAAGAAATCACCAAAGGTTTACT
CTCGGCTTGTGACTCTTCTTCTTATGAAATCATGGTCTTCCCGAGTGCCCTCCATTTGGAGTCAGTGGCATCCATTGCCC
GAGACTCCCAACTCATCGTGGGGGCACAAAATGCCTACCAATCGGGGCTTACCGCCATGACAGGTGAAATCTCCCCAGTC
CAGCTGGCAGAACTGGGCATCCAAACCGTTCTTGTGGGCCACTCGGAAAGGAGACAATTCCTCGGAGAAACATCCGAATT
TGACAATACCAAAATCTCCTACTTCTTAAAACATGGACTACGAGTTGTCTATTGTGTCGGGGAAACTTGGGCCGAACGAG
AAAAAGGAAACACCTTTTCTGTGTTAGAGGACCAAATTGGCAAAGGATTAAAAGGGATCACAAGTGACCTTTTCAAAAAC
CTCGTGATTGCCTATGAACCAGTTTGGGCCATTGGAACGGGAAAAGTAGCAACTCCCGTAGAAGCGGAAGAAGCGCATGC
CTTTATCCGAAAAGAAATTGGCAAATTATTTGTTGGTGCAGACCTTGTGGCGGAGAACATTCAAATTCTTTATGGTGGTT
CAGTCAAACCTGACAACATCAAGGAACTTCTCGCCAAACCAAATATTGACGGTGGCCTCGTTGGTGGAGCCAGTCAAAAA
TTGGATTTATTTTTAGGACTTTTAAAATAA

Upstream 100 bases:

>100_bases
ATCACTCATATTTCCACAGGTGGAGGTGCTTCCTTAGAATTTTTGGAAGGACGAACTCTCCCTGGCGTACAATGTTTACT
CCCAAAGGAAGAAAAATAAA

Downstream 100 bases:

>100_bases
GGATACTTTATGGGATTTTTTGCAGGAACCATTCTCACTCTATTTGTTCTTCTCTCTCTTTTTCTCATCCTACTTGTGAT
GATCCAAACAGGAAAAGGTG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRKKIIAGNWKMNLTLAEAKEITKGLLSACDSSSYEIMVFPSALHLESVASIARDSQLIVGAQNAYQSGLTAMTGEISPV
QLAELGIQTVLVGHSERRQFLGETSEFDNTKISYFLKHGLRVVYCVGETWAEREKGNTFSVLEDQIGKGLKGITSDLFKN
LVIAYEPVWAIGTGKVATPVEAEEAHAFIRKEIGKLFVGADLVAENIQILYGGSVKPDNIKELLAKPNIDGGLVGGASQK
LDLFLGLLK

Sequences:

>Translated_249_residues
MRKKIIAGNWKMNLTLAEAKEITKGLLSACDSSSYEIMVFPSALHLESVASIARDSQLIVGAQNAYQSGLTAMTGEISPV
QLAELGIQTVLVGHSERRQFLGETSEFDNTKISYFLKHGLRVVYCVGETWAEREKGNTFSVLEDQIGKGLKGITSDLFKN
LVIAYEPVWAIGTGKVATPVEAEEAHAFIRKEIGKLFVGADLVAENIQILYGGSVKPDNIKELLAKPNIDGGLVGGASQK
LDLFLGLLK
>Mature_249_residues
MRKKIIAGNWKMNLTLAEAKEITKGLLSACDSSSYEIMVFPSALHLESVASIARDSQLIVGAQNAYQSGLTAMTGEISPV
QLAELGIQTVLVGHSERRQFLGETSEFDNTKISYFLKHGLRVVYCVGETWAEREKGNTFSVLEDQIGKGLKGITSDLFKN
LVIAYEPVWAIGTGKVATPVEAEEAHAFIRKEIGKLFVGADLVAENIQILYGGSVKPDNIKELLAKPNIDGGLVGGASQK
LDLFLGLLK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=39.7540983606557, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=39.7540983606557, Blast_Score=161, Evalue=4e-40,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=40.4, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=43.0894308943089, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320255, Length=239, Percent_Identity=39.7489539748954, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28572008, Length=242, Percent_Identity=42.5619834710744, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28572006, Length=242, Percent_Identity=42.5619834710744, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28572004, Length=242, Percent_Identity=42.5619834710744, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LEPBA (B0SB22)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963106.1
- ProteinModelPortal:   B0SB22
- SMR:   B0SB22
- GeneID:   6388562
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2028
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   LBIF355278:LBF_2028-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26969; Mature: 26969

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIIAGNWKMNLTLAEAKEITKGLLSACDSSSYEIMVFPSALHLESVASIARDSQLIV
CCCCEEECCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCEEEE
GAQNAYQSGLTAMTGEISPVQLAELGIQTVLVGHSERRQFLGETSEFDNTKISYFLKHGL
ECCHHHHCCCHHHCCCCCCHHHHHHCHHHEEECCHHHHHHCCCCCCCCCCHHHHHHHCCC
RVVYCVGETWAEREKGNTFSVLEDQIGKGLKGITSDLFKNLVIAYEPVWAIGTGKVATPV
EEEEECCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEEECCCCCCCCC
EAEEAHAFIRKEIGKLFVGADLVAENIQILYGGSVKPDNIKELLAKPNIDGGLVGGASQK
CHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCEECCCCHH
LDLFLGLLK
HHHHHHHCC
>Mature Secondary Structure
MRKKIIAGNWKMNLTLAEAKEITKGLLSACDSSSYEIMVFPSALHLESVASIARDSQLIV
CCCCEEECCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCEEEE
GAQNAYQSGLTAMTGEISPVQLAELGIQTVLVGHSERRQFLGETSEFDNTKISYFLKHGL
ECCHHHHCCCHHHCCCCCCHHHHHHCHHHEEECCHHHHHHCCCCCCCCCCHHHHHHHCCC
RVVYCVGETWAEREKGNTFSVLEDQIGKGLKGITSDLFKNLVIAYEPVWAIGTGKVATPV
EEEEECCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEEECCCCCCCCC
EAEEAHAFIRKEIGKLFVGADLVAENIQILYGGSVKPDNIKELLAKPNIDGGLVGGASQK
CHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCEECCCCHH
LDLFLGLLK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA