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The map label for this gene is pgk

Identifier: 183221463

GI number: 183221463

Start: 2175861

End: 2177051

Strand: Direct

Name: pgk

Synonym: LEPBI_I2081

Alternate gene names: 183221463

Gene position: 2175861-2177051 (Clockwise)

Preceding gene: 183221462

Following gene: 183221464

Centisome position: 60.45

GC content: 45.26

Gene sequence:

>1191_bases
ATGAAATTACCTCTTCTCGAAGAACAAAATCTAAAAGGAAAACGAGTCTTTGTTCGTGTGGATTTCAATGTCCCTGTGGA
AAACGGAAAAGCAACGGACAGAACTCGGATTGAAAAAACCCTCCCTACTTTGGAACTTCTGATTTCCAAAGGTGCTAAAA
TCATTTTGGGAAGCCACTTAGGTCGCCCGAAAGGTGGACCGGAACCGAAGTATTCCATGAAACCGGTGTTTGATGTTCTT
TCCGAACTTGTCAAAACCAAAGTGAGTTTCTCGGAATCTGTGATTGGTTCCGATGTGGTGAAAATGACGAATGCGCTTGG
GGAAGGTGAAATCCTACTTTTAGAAAACCTTCGGTTCCATAAGGAAGAAGAGGAAAATGTTGCCAGTTTCTGTAAGGAAC
TGGCAAAACTCGCTGATGTTTATGTCAATGATGCGTTCGGAACGGCACACAGAGCACATGCTTCGACAGAAGGTGTGGCC
CACCTACTCCCTGCCTTTGCGGGACTACTCATGCGAAAAGAAATTGAAGTCCTCAGTGGCCTACTCGCAAAACCTGAGCG
TCCTTTTGTGGCTATTGTGGGTGGATCCAAAGTCAGTTCCAAATTTGCCATTTTAAAAAACCTCCTCGAGAAGGTAGACC
ACCTCCTCATTGGTGGGGGTATGGCATACACGTTTTTAAAATCAAGAGCGGTTCCTGTGGGAAAATCCCTTGTGGAACCA
GAATTTGAATCCCAAGCCTTCCAACTCATTGACCGAGCTGGGATCCAAGGGGTTGACCTGCAAATTCCAGTGGACCACAT
CATTGCGGATGCCTTTGATCCCAATGCCAAAACAAAGTCTGTGGACAAAATGGGAATCATCGATGGTTGGATGGGAATGG
ACATTGGACCAAAGACCATCGACAACTATGTCAAAGCGATAAAAGATGCCAAAACCATTTTATGGAACGGCCCAATGGGT
GTCTTTGAAATGGATAAGTTCTCAAAAGGAACCATCGAAATCGCCAAAGCCATCAGTAAATCCAAGGCCAAAACCGTTGT
GGGTGGTGGTGATTCCATTGCCGCTGTGAACAAAGCGGGAGTAGCGGACAAAATCACTCATATTTCCACAGGTGGAGGTG
CTTCCTTAGAATTTTTGGAAGGACGAACTCTCCCTGGCGTACAATGTTTACTCCCAAAGGAAGAAAAATAA

Upstream 100 bases:

>100_bases
AGCCCTACTTTTTTCAAACTCGTATCTTGGTATGACAATGAAATGGGATACTCCAACCGAGTCCTCGACCTAGTTCGTTA
TATGGCAAAAAAAGGCTAAG

Downstream 100 bases:

>100_bases
AATGAGAAAGAAGATCATCGCAGGGAATTGGAAAATGAACCTCACGCTAGCGGAGGCAAAAGAAATCACCAAAGGTTTAC
TCTCGGCTTGTGACTCTTCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKLPLLEEQNLKGKRVFVRVDFNVPVENGKATDRTRIEKTLPTLELLISKGAKIILGSHLGRPKGGPEPKYSMKPVFDVL
SELVKTKVSFSESVIGSDVVKMTNALGEGEILLLENLRFHKEEEENVASFCKELAKLADVYVNDAFGTAHRAHASTEGVA
HLLPAFAGLLMRKEIEVLSGLLAKPERPFVAIVGGSKVSSKFAILKNLLEKVDHLLIGGGMAYTFLKSRAVPVGKSLVEP
EFESQAFQLIDRAGIQGVDLQIPVDHIIADAFDPNAKTKSVDKMGIIDGWMGMDIGPKTIDNYVKAIKDAKTILWNGPMG
VFEMDKFSKGTIEIAKAISKSKAKTVVGGGDSIAAVNKAGVADKITHISTGGGASLEFLEGRTLPGVQCLLPKEEK

Sequences:

>Translated_396_residues
MKLPLLEEQNLKGKRVFVRVDFNVPVENGKATDRTRIEKTLPTLELLISKGAKIILGSHLGRPKGGPEPKYSMKPVFDVL
SELVKTKVSFSESVIGSDVVKMTNALGEGEILLLENLRFHKEEEENVASFCKELAKLADVYVNDAFGTAHRAHASTEGVA
HLLPAFAGLLMRKEIEVLSGLLAKPERPFVAIVGGSKVSSKFAILKNLLEKVDHLLIGGGMAYTFLKSRAVPVGKSLVEP
EFESQAFQLIDRAGIQGVDLQIPVDHIIADAFDPNAKTKSVDKMGIIDGWMGMDIGPKTIDNYVKAIKDAKTILWNGPMG
VFEMDKFSKGTIEIAKAISKSKAKTVVGGGDSIAAVNKAGVADKITHISTGGGASLEFLEGRTLPGVQCLLPKEEK
>Mature_396_residues
MKLPLLEEQNLKGKRVFVRVDFNVPVENGKATDRTRIEKTLPTLELLISKGAKIILGSHLGRPKGGPEPKYSMKPVFDVL
SELVKTKVSFSESVIGSDVVKMTNALGEGEILLLENLRFHKEEEENVASFCKELAKLADVYVNDAFGTAHRAHASTEGVA
HLLPAFAGLLMRKEIEVLSGLLAKPERPFVAIVGGSKVSSKFAILKNLLEKVDHLLIGGGMAYTFLKSRAVPVGKSLVEP
EFESQAFQLIDRAGIQGVDLQIPVDHIIADAFDPNAKTKSVDKMGIIDGWMGMDIGPKTIDNYVKAIKDAKTILWNGPMG
VFEMDKFSKGTIEIAKAISKSKAKTVVGGGDSIAAVNKAGVADKITHISTGGGASLEFLEGRTLPGVQCLLPKEEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=44.4717444717445, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=44.2260442260442, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1789294, Length=401, Percent_Identity=42.8927680798005, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=46.0975609756098, Blast_Score=358, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=44.7432762836186, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=44.0886699507389, Blast_Score=310, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24581203, Length=407, Percent_Identity=41.031941031941, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_LEPBA (B0SB21)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963105.1
- ProteinModelPortal:   B0SB21
- SMR:   B0SB21
- GeneID:   6388573
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2027
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   LBIF355278:LBF_2027-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42739; Mature: 42739

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 292-292 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPLLEEQNLKGKRVFVRVDFNVPVENGKATDRTRIEKTLPTLELLISKGAKIILGSHL
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
GRPKGGPEPKYSMKPVFDVLSELVKTKVSFSESVIGSDVVKMTNALGEGEILLLENLRFH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KEEEENVASFCKELAKLADVYVNDAFGTAHRAHASTEGVAHLLPAFAGLLMRKEIEVLSG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLAKPERPFVAIVGGSKVSSKFAILKNLLEKVDHLLIGGGMAYTFLKSRAVPVGKSLVEP
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCC
EFESQAFQLIDRAGIQGVDLQIPVDHIIADAFDPNAKTKSVDKMGIIDGWMGMDIGPKTI
CHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHH
DNYVKAIKDAKTILWNGPMGVFEMDKFSKGTIEIAKAISKSKAKTVVGGGDSIAAVNKAG
HHHHHHHHHCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHCCEEECCCCCEEHHHHCC
VADKITHISTGGGASLEFLEGRTLPGVQCLLPKEEK
CHHHHHEEECCCCCCEEEECCCCCCCEEECCCCCCC
>Mature Secondary Structure
MKLPLLEEQNLKGKRVFVRVDFNVPVENGKATDRTRIEKTLPTLELLISKGAKIILGSHL
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
GRPKGGPEPKYSMKPVFDVLSELVKTKVSFSESVIGSDVVKMTNALGEGEILLLENLRFH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
KEEEENVASFCKELAKLADVYVNDAFGTAHRAHASTEGVAHLLPAFAGLLMRKEIEVLSG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLAKPERPFVAIVGGSKVSSKFAILKNLLEKVDHLLIGGGMAYTFLKSRAVPVGKSLVEP
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHCCC
EFESQAFQLIDRAGIQGVDLQIPVDHIIADAFDPNAKTKSVDKMGIIDGWMGMDIGPKTI
CHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHH
DNYVKAIKDAKTILWNGPMGVFEMDKFSKGTIEIAKAISKSKAKTVVGGGDSIAAVNKAG
HHHHHHHHHCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHCCEEECCCCCEEHHHHCC
VADKITHISTGGGASLEFLEGRTLPGVQCLLPKEEK
CHHHHHEEECCCCCCEEEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA