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The map label for this gene is fixL [H]

Identifier: 183221437

GI number: 183221437

Start: 2142528

End: 2144456

Strand: Direct

Name: fixL [H]

Synonym: LEPBI_I2055

Alternate gene names: 183221437

Gene position: 2142528-2144456 (Clockwise)

Preceding gene: 183221436

Following gene: 183221438

Centisome position: 59.52

GC content: 35.2

Gene sequence:

>1929_bases
ATGAATTGTTTCTGCATAGTTATGAGCCAAACGATCCTTCCATCAGAGTTCTTTTTTACTTTTGCAAACACTCTCCCCTA
TTCTTTATTTTTATTTGAATCAAAATCCCAAACATTTGCCAAAGACAATCCCTTACATTTTTCGAACATAAAAGCAAAAT
CACTCCACCATTTGCTCCAAGTTCCAAATAAGACCAATACGATAGGATCCATGTTTCCTTTTTTATTTGAATCGAATCTT
CAATTACAAATTCAAAGCGACATACAGAATCAAAAACCGTTTGAATCAGTCATCGCAAAAGAAAAGTTACACTTCTATGG
ATTTCAAAACCATCCGTATCTTTTGAAACTTACCCAACTAGATTTCAATTTTTATACGATCACCATTGAAGAATTAACGG
ATGCGGTACTTGCCGAAAAAATCCTAAAACAAAAAGAATCAAAACTCGATCGTTTATTAAAAACAATGATTAATGGTGTG
GTTGTAGTGAACAAGGAAGGACAAATTCTCTATGCCAATGATAGTGCTTCCGAAATTTTAGAATTAGAATTGGCGAGTAT
CGAAAATCGTTATTTTAGTTCCAGAGAATGGAAACAAATTTATGAAGATGGTAGCCCTTATCCCACAGATAAACTTCCGT
TAGCCCAAGCTCTTGGAGATCAACTCACTGTTTATAATTGTGAACACGGAATCCTTTCAGAAGAAGGTCATGTCAAATGG
CTAAATGTCAATGCAACACCACTCTTTGATGAATACGGTAATTTAGAAGGGGCAACTGCGAGTTTTTTGGACATCACTGA
ATTAAAAAAAAACCAAACCACCATTGAACAACAAAATAAAAAATTAAAATCTGTCCTTGAGGCAATCGAAAAATCAGCCA
TTGTCAGTGTCACAAATCCAGATGGGATCATCATCAGGGCAAATTCAAAATTCATCCAAATCACAGGTTTTCCTGAAAAT
GAAATCATAGGATCTGATCATAAAATATTCAGTTCCGATTACCACAAAAAAGAATTTTGGAAAGGATTATGGAATCAGAT
CAAACATGGGAAAACATGGGAGGGTATCATCAAAAACAAATCCAAAGATGGGAACTATTTCTGGCTTCAAACCTTTATCC
ATCCATTATTTAACTCAGAAGAAAAAATTGAAGCCTATCTTTCCATTCGGTTTGATATCACAGAAGAAATTGTGGCATTA
GAAAACACAAAACGAATGTTACACTTCACTGGCATTCAAAACAATCGCCTTCAAAACTTTGCATATATCCTCTCCCACAA
CATCAGGCAACACTCATCAAACTTCACATCCCTCATTCAATTATTAGAAGAATCCAAATCAGAAGTAGATAAAAAAAACA
TCGTAGAAATGTTACATGCCTCATCAGTGAAATTGGAAGAGACCATTTCCCACTTAAACGATATCATCTCTATCAACCAA
ACCTTAAATCAACCAATGGAAATTTGTTCATTAAAAAATGAGGTAAACAAAACGCTCTCCATCTTAAGTGGATCCATCGA
ACATAGAAATATAAAAATCATTACGATCATCCCTGACGAACTCAAAGTCAAAACCATACCAAATTACTTAGAAAGTATTT
TACTAAACCTATTATCCAATGCTGTGAAATATGTTCGTTTGAAGGAAGGGGCTTGGATCAAAATCCAAATCGAGGAAAAC
CAGGACCAAATTCAAATCAAAGTCGAAGACAACGGACTGGGTATCAATTTAGAAAAACATGGAAATAAAATATTTGGTAT
GTTTAAAACCTTTCATCGAAATGAAGATGCAAGAGGGATTGGACTTTTCATCACAAAGTCCCAAGTGGAAGTGCTTGGAG
GTAAAATCACCCTGCAAAGCGAAGAAGGAAAAGGTTCCCTTTTTTCTGTGAGCCTCCCCAAAAATCCAGAAGAAAGCCTC
CGGGTTTAG

Upstream 100 bases:

>100_bases
TTACGACTTTCTCCGTATTGGAACAAATTTTAAAGGAAACGATTCTAAAACCAAACGATTTCATTCGTCGAATCAATTCT
GAATGTGAAACAATTCTCTT

Downstream 100 bases:

>100_bases
GATGTTAGATTTTTAAGCAATGTTCGTCTACCGATTCTCTTTCGTCCCTCTTTCCTTACTTCCTTTCAATCCGTCTTGAC
AATCTCACCCAACCCTTGAG

Product: putative sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MNCFCIVMSQTILPSEFFFTFANTLPYSLFLFESKSQTFAKDNPLHFSNIKAKSLHHLLQVPNKTNTIGSMFPFLFESNL
QLQIQSDIQNQKPFESVIAKEKLHFYGFQNHPYLLKLTQLDFNFYTITIEELTDAVLAEKILKQKESKLDRLLKTMINGV
VVVNKEGQILYANDSASEILELELASIENRYFSSREWKQIYEDGSPYPTDKLPLAQALGDQLTVYNCEHGILSEEGHVKW
LNVNATPLFDEYGNLEGATASFLDITELKKNQTTIEQQNKKLKSVLEAIEKSAIVSVTNPDGIIIRANSKFIQITGFPEN
EIIGSDHKIFSSDYHKKEFWKGLWNQIKHGKTWEGIIKNKSKDGNYFWLQTFIHPLFNSEEKIEAYLSIRFDITEEIVAL
ENTKRMLHFTGIQNNRLQNFAYILSHNIRQHSSNFTSLIQLLEESKSEVDKKNIVEMLHASSVKLEETISHLNDIISINQ
TLNQPMEICSLKNEVNKTLSILSGSIEHRNIKIITIIPDELKVKTIPNYLESILLNLLSNAVKYVRLKEGAWIKIQIEEN
QDQIQIKVEDNGLGINLEKHGNKIFGMFKTFHRNEDARGIGLFITKSQVEVLGGKITLQSEEGKGSLFSVSLPKNPEESL
RV

Sequences:

>Translated_642_residues
MNCFCIVMSQTILPSEFFFTFANTLPYSLFLFESKSQTFAKDNPLHFSNIKAKSLHHLLQVPNKTNTIGSMFPFLFESNL
QLQIQSDIQNQKPFESVIAKEKLHFYGFQNHPYLLKLTQLDFNFYTITIEELTDAVLAEKILKQKESKLDRLLKTMINGV
VVVNKEGQILYANDSASEILELELASIENRYFSSREWKQIYEDGSPYPTDKLPLAQALGDQLTVYNCEHGILSEEGHVKW
LNVNATPLFDEYGNLEGATASFLDITELKKNQTTIEQQNKKLKSVLEAIEKSAIVSVTNPDGIIIRANSKFIQITGFPEN
EIIGSDHKIFSSDYHKKEFWKGLWNQIKHGKTWEGIIKNKSKDGNYFWLQTFIHPLFNSEEKIEAYLSIRFDITEEIVAL
ENTKRMLHFTGIQNNRLQNFAYILSHNIRQHSSNFTSLIQLLEESKSEVDKKNIVEMLHASSVKLEETISHLNDIISINQ
TLNQPMEICSLKNEVNKTLSILSGSIEHRNIKIITIIPDELKVKTIPNYLESILLNLLSNAVKYVRLKEGAWIKIQIEEN
QDQIQIKVEDNGLGINLEKHGNKIFGMFKTFHRNEDARGIGLFITKSQVEVLGGKITLQSEEGKGSLFSVSLPKNPEESL
RV
>Mature_642_residues
MNCFCIVMSQTILPSEFFFTFANTLPYSLFLFESKSQTFAKDNPLHFSNIKAKSLHHLLQVPNKTNTIGSMFPFLFESNL
QLQIQSDIQNQKPFESVIAKEKLHFYGFQNHPYLLKLTQLDFNFYTITIEELTDAVLAEKILKQKESKLDRLLKTMINGV
VVVNKEGQILYANDSASEILELELASIENRYFSSREWKQIYEDGSPYPTDKLPLAQALGDQLTVYNCEHGILSEEGHVKW
LNVNATPLFDEYGNLEGATASFLDITELKKNQTTIEQQNKKLKSVLEAIEKSAIVSVTNPDGIIIRANSKFIQITGFPEN
EIIGSDHKIFSSDYHKKEFWKGLWNQIKHGKTWEGIIKNKSKDGNYFWLQTFIHPLFNSEEKIEAYLSIRFDITEEIVAL
ENTKRMLHFTGIQNNRLQNFAYILSHNIRQHSSNFTSLIQLLEESKSEVDKKNIVEMLHASSVKLEETISHLNDIISINQ
TLNQPMEICSLKNEVNKTLSILSGSIEHRNIKIITIIPDELKVKTIPNYLESILLNLLSNAVKYVRLKEGAWIKIQIEEN
QDQIQIKVEDNGLGINLEKHGNKIFGMFKTFHRNEDARGIGLFITKSQVEVLGGKITLQSEEGKGSLFSVSLPKNPEESL
RV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=395, Percent_Identity=25.5696202531646, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789453, Length=128, Percent_Identity=32.03125, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081816, Length=272, Percent_Identity=25, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=366, Percent_Identity=23.4972677595628, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=25.7918552036199, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=271, Percent_Identity=23.6162361623616, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73513; Mature: 73513

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCFCIVMSQTILPSEFFFTFANTLPYSLFLFESKSQTFAKDNPLHFSNIKAKSLHHLLQ
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHH
VPNKTNTIGSMFPFLFESNLQLQIQSDIQNQKPFESVIAKEKLHFYGFQNHPYLLKLTQL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCEEEEEEEE
DFNFYTITIEELTDAVLAEKILKQKESKLDRLLKTMINGVVVVNKEGQILYANDSASEIL
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHH
ELELASIENRYFSSREWKQIYEDGSPYPTDKLPLAQALGDQLTVYNCEHGILSEEGHVKW
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCEEE
LNVNATPLFDEYGNLEGATASFLDITELKKNQTTIEQQNKKLKSVLEAIEKSAIVSVTNP
EECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGIIIRANSKFIQITGFPENEIIGSDHKIFSSDYHKKEFWKGLWNQIKHGKTWEGIIKNK
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCC
SKDGNYFWLQTFIHPLFNSEEKIEAYLSIRFDITEEIVALENTKRMLHFTGIQNNRLQNF
CCCCCEEEHHHHHHHHHCCHHHEEEEEEEEEECHHHHHHHHCCCEEEEEECCCCCCHHHH
AYILSHNIRQHSSNFTSLIQLLEESKSEVDKKNIVEMLHASSVKLEETISHLNDIISINQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLNQPMEICSLKNEVNKTLSILSGSIEHRNIKIITIIPDELKVKTIPNYLESILLNLLSN
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
AVKYVRLKEGAWIKIQIEENQDQIQIKVEDNGLGINLEKHGNKIFGMFKTFHRNEDARGI
HHHEEEEECCCEEEEEEECCCCEEEEEEECCCEEEEEHHCCCCHHHHHHHHHCCCCCCEE
GLFITKSQVEVLGGKITLQSEEGKGSLFSVSLPKNPEESLRV
EEEEECCCCEEECCEEEEECCCCCCCEEEEECCCCCHHHCCC
>Mature Secondary Structure
MNCFCIVMSQTILPSEFFFTFANTLPYSLFLFESKSQTFAKDNPLHFSNIKAKSLHHLLQ
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHH
VPNKTNTIGSMFPFLFESNLQLQIQSDIQNQKPFESVIAKEKLHFYGFQNHPYLLKLTQL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCEEEEEEEE
DFNFYTITIEELTDAVLAEKILKQKESKLDRLLKTMINGVVVVNKEGQILYANDSASEIL
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCHHHHH
ELELASIENRYFSSREWKQIYEDGSPYPTDKLPLAQALGDQLTVYNCEHGILSEEGHVKW
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCEEE
LNVNATPLFDEYGNLEGATASFLDITELKKNQTTIEQQNKKLKSVLEAIEKSAIVSVTNP
EECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
DGIIIRANSKFIQITGFPENEIIGSDHKIFSSDYHKKEFWKGLWNQIKHGKTWEGIIKNK
CEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCC
SKDGNYFWLQTFIHPLFNSEEKIEAYLSIRFDITEEIVALENTKRMLHFTGIQNNRLQNF
CCCCCEEEHHHHHHHHHCCHHHEEEEEEEEEECHHHHHHHHCCCEEEEEECCCCCCHHHH
AYILSHNIRQHSSNFTSLIQLLEESKSEVDKKNIVEMLHASSVKLEETISHLNDIISINQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLNQPMEICSLKNEVNKTLSILSGSIEHRNIKIITIIPDELKVKTIPNYLESILLNLLSN
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
AVKYVRLKEGAWIKIQIEENQDQIQIKVEDNGLGINLEKHGNKIFGMFKTFHRNEDARGI
HHHEEEEECCCEEEEEEECCCCEEEEEEECCCEEEEEHHCCCCHHHHHHHHHCCCCCCEE
GLFITKSQVEVLGGKITLQSEEGKGSLFSVSLPKNPEESLRV
EEEEECCCCEEECCEEEEECCCCCCCEEEEECCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]