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The map label for this gene is dctB [H]

Identifier: 183221320

GI number: 183221320

Start: 2025659

End: 2027365

Strand: Reverse

Name: dctB [H]

Synonym: LEPBI_I1937

Alternate gene names: 183221320

Gene position: 2027365-2025659 (Counterclockwise)

Preceding gene: 183221321

Following gene: 183221318

Centisome position: 56.32

GC content: 39.48

Gene sequence:

>1707_bases
GTGTATTGCAGGTGGCTTGGGCGAAATAGGGATGAGGTAATTGGAAAAACGACAGTGGATTTGGGTATTTATTCAAATCT
CGAAGATCGGGAAATAATCCTTTCGAAATTAAATTCAGAAGGTTTTGTTTTGAACTTTGAAGTTCCGCTCGTCACAAAAA
CGGGTGAGACGGTCACAATTCTATTCAGTGGGAAAATTGTGGAAGGTGGCAAATACCTTCTGAGTGCGGGACAAAACATC
TCGGCATTGAAAGAAAAAGAAACACTAGCCAATGCCCTCCAAAAAGAATTAAAAATCAGCAAAGAATTATTTGAAAGTGT
GTTTAGGTTAAACCCTGCAGCTGTCAGTTTATCGAATGCAGAAACAGGAAGATACGATGATGTCAATGAAGCGTATTGCC
GACTCGTCGGATTTGACCGTGAAGAAATCATCGGACGAACCTCACATGAATTGAATATTTGGATCACCAAATTGGATCGT
GCGAGGTTATTGGCCGAAGTCCAAAAAAAAGGTTGGAGTACGGGAATGGAAGCAAGTCTCCGAATGAAATCTGGAGAAAT
TCGGCATATTGTATCGGGTAATACCATACTCAACCACGATGGACGTTCTACCCTCCTTGCAATCCTCATTGATATTACAG
AAGCCAAACAAAACAAAGAAGCACTCGAATTTGCAGTCAAAGAACGGACGAAGGATCTTAATCGGATCCTGGAAGATTTA
CAAAAAACCCAAGACCAATTGATCCTTTCCGAAAAAATGGCAACCCTTGGCCAACTTGTCGCGAGTGTCGCACATGAAAT
TAATAACCCTCTTGCGGCAATCTCAGCCTTTAGTGAACAATTACAAAACCGATTGGGTGATTTTGCATCTCGTCTTCTGG
AAATCAGGAATTGTATGGGGAAATATTCTGATGAGGATGCAGAAGGGATCATTCAATGGATTTCCGAATTATTCCAAGCG
AAACCAAAAACGCATAGTTTTGCCGAAACAAGGAAAATTAAAAAGAATTTAGAAGCCTTATTTGTATCAGTAAGCATCGA
TTCGCCATATGATTTAGCGGACCGTATCGTTGATTTAGGCGTTTCCGATTTTATATTAGAAAATACTAATTTTGTTGTGA
AACTTAAAAACACCCCCATTTTAAGTATCATTTTGAACGAACTCTATTCTTTACGAAGCATTGAATCCATTCGTTTGGCA
GTGGAACGTACTTCAAAGATTGTTTATAGTTTAAAAAATTATGGAAGAATGGACCGTGGATCTGCAAAAATCCAGATCAA
TATCATTGATACAATTGAAACTGTTCTTACTTTGTATCAAAATAAAATGAAGTCAGGGATTGAGTGCATTCGTTTGTACA
ATGCCAACCCTATGATTATGGGGTATCCAGATGAACTCATCCAAATCTGGACCAACTTGATTTATAATGCATTACAAGCG
ATGCACTTTAAAGGGAAATTAACCATTCAAGTAGAAGAAACTGGAACTGATGTTGAGGTCTCCATCAAAGACGATGGCCC
TGGAATCCCATTGGCTGTGCAAAGGCGGATCTTTGAACCTTTTTTTACCACAAAAGAAAAAGGAGAAGGGACTGGGCTTG
GCCTCGGGATTGTCAAACAATCAGTGGAAGAAAGGCATAAAGGCCAAATTCAATTGTTTTCCGAACCTGGCCAGACTGTT
TTTCTCGTTTCTCTTCCTAAACTCTAG

Upstream 100 bases:

>100_bases
ACCTATTTCTGATCCCATTTGGCAAACCAGCTTCATTGAAAGTCCTATTGGGATGGCCATCACTGATATGCAAACTGGAC
TTTATGTTGAGGCAAATGAG

Downstream 100 bases:

>100_bases
CCAACTGCTTCTGCAATTCTTCTGCGACGACGTCAAAGTTTTGATTTTGGATGACAGTTTTGTAAGCGGAAGCGACGATC
GGGTGCCTTTTTTCGTCAAG

Product: putative histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MYCRWLGRNRDEVIGKTTVDLGIYSNLEDREIILSKLNSEGFVLNFEVPLVTKTGETVTILFSGKIVEGGKYLLSAGQNI
SALKEKETLANALQKELKISKELFESVFRLNPAAVSLSNAETGRYDDVNEAYCRLVGFDREEIIGRTSHELNIWITKLDR
ARLLAEVQKKGWSTGMEASLRMKSGEIRHIVSGNTILNHDGRSTLLAILIDITEAKQNKEALEFAVKERTKDLNRILEDL
QKTQDQLILSEKMATLGQLVASVAHEINNPLAAISAFSEQLQNRLGDFASRLLEIRNCMGKYSDEDAEGIIQWISELFQA
KPKTHSFAETRKIKKNLEALFVSVSIDSPYDLADRIVDLGVSDFILENTNFVVKLKNTPILSIILNELYSLRSIESIRLA
VERTSKIVYSLKNYGRMDRGSAKIQINIIDTIETVLTLYQNKMKSGIECIRLYNANPMIMGYPDELIQIWTNLIYNALQA
MHFKGKLTIQVEETGTDVEVSIKDDGPGIPLAVQRRIFEPFFTTKEKGEGTGLGLGIVKQSVEERHKGQIQLFSEPGQTV
FLVSLPKL

Sequences:

>Translated_568_residues
MYCRWLGRNRDEVIGKTTVDLGIYSNLEDREIILSKLNSEGFVLNFEVPLVTKTGETVTILFSGKIVEGGKYLLSAGQNI
SALKEKETLANALQKELKISKELFESVFRLNPAAVSLSNAETGRYDDVNEAYCRLVGFDREEIIGRTSHELNIWITKLDR
ARLLAEVQKKGWSTGMEASLRMKSGEIRHIVSGNTILNHDGRSTLLAILIDITEAKQNKEALEFAVKERTKDLNRILEDL
QKTQDQLILSEKMATLGQLVASVAHEINNPLAAISAFSEQLQNRLGDFASRLLEIRNCMGKYSDEDAEGIIQWISELFQA
KPKTHSFAETRKIKKNLEALFVSVSIDSPYDLADRIVDLGVSDFILENTNFVVKLKNTPILSIILNELYSLRSIESIRLA
VERTSKIVYSLKNYGRMDRGSAKIQINIIDTIETVLTLYQNKMKSGIECIRLYNANPMIMGYPDELIQIWTNLIYNALQA
MHFKGKLTIQVEETGTDVEVSIKDDGPGIPLAVQRRIFEPFFTTKEKGEGTGLGLGIVKQSVEERHKGQIQLFSEPGQTV
FLVSLPKL
>Mature_568_residues
MYCRWLGRNRDEVIGKTTVDLGIYSNLEDREIILSKLNSEGFVLNFEVPLVTKTGETVTILFSGKIVEGGKYLLSAGQNI
SALKEKETLANALQKELKISKELFESVFRLNPAAVSLSNAETGRYDDVNEAYCRLVGFDREEIIGRTSHELNIWITKLDR
ARLLAEVQKKGWSTGMEASLRMKSGEIRHIVSGNTILNHDGRSTLLAILIDITEAKQNKEALEFAVKERTKDLNRILEDL
QKTQDQLILSEKMATLGQLVASVAHEINNPLAAISAFSEQLQNRLGDFASRLLEIRNCMGKYSDEDAEGIIQWISELFQA
KPKTHSFAETRKIKKNLEALFVSVSIDSPYDLADRIVDLGVSDFILENTNFVVKLKNTPILSIILNELYSLRSIESIRLA
VERTSKIVYSLKNYGRMDRGSAKIQINIIDTIETVLTLYQNKMKSGIECIRLYNANPMIMGYPDELIQIWTNLIYNALQA
MHFKGKLTIQVEETGTDVEVSIKDDGPGIPLAVQRRIFEPFFTTKEKGEGTGLGLGIVKQSVEERHKGQIQLFSEPGQTV
FLVSLPKL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=118, Percent_Identity=41.5254237288136, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790300, Length=146, Percent_Identity=33.5616438356164, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=170, Percent_Identity=32.9411764705882, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63882; Mature: 63882

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYCRWLGRNRDEVIGKTTVDLGIYSNLEDREIILSKLNSEGFVLNFEVPLVTKTGETVTI
CCCEECCCCHHHHCCCEEEEEHCCCCCCHHHHHHHHCCCCCEEEEEEEEEEEECCCEEEE
LFSGKIVEGGKYLLSAGQNISALKEKETLANALQKELKISKELFESVFRLNPAAVSLSNA
EEECEEECCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
ETGRYDDVNEAYCRLVGFDREEIIGRTSHELNIWITKLDRARLLAEVQKKGWSTGMEASL
CCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCEE
RMKSGEIRHIVSGNTILNHDGRSTLLAILIDITEAKQNKEALEFAVKERTKDLNRILEDL
EECCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QKTQDQLILSEKMATLGQLVASVAHEINNPLAAISAFSEQLQNRLGDFASRLLEIRNCMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KYSDEDAEGIIQWISELFQAKPKTHSFAETRKIKKNLEALFVSVSIDSPYDLADRIVDLG
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHCC
VSDFILENTNFVVKLKNTPILSIILNELYSLRSIESIRLAVERTSKIVYSLKNYGRMDRG
CHHHEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SAKIQINIIDTIETVLTLYQNKMKSGIECIRLYNANPMIMGYPDELIQIWTNLIYNALQA
CCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHH
MHFKGKLTIQVEETGTDVEVSIKDDGPGIPLAVQRRIFEPFFTTKEKGEGTGLGLGIVKQ
HEECCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SVEERHKGQIQLFSEPGQTVFLVSLPKL
HHHHHCCCCEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MYCRWLGRNRDEVIGKTTVDLGIYSNLEDREIILSKLNSEGFVLNFEVPLVTKTGETVTI
CCCEECCCCHHHHCCCEEEEEHCCCCCCHHHHHHHHCCCCCEEEEEEEEEEEECCCEEEE
LFSGKIVEGGKYLLSAGQNISALKEKETLANALQKELKISKELFESVFRLNPAAVSLSNA
EEECEEECCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
ETGRYDDVNEAYCRLVGFDREEIIGRTSHELNIWITKLDRARLLAEVQKKGWSTGMEASL
CCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCEE
RMKSGEIRHIVSGNTILNHDGRSTLLAILIDITEAKQNKEALEFAVKERTKDLNRILEDL
EECCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QKTQDQLILSEKMATLGQLVASVAHEINNPLAAISAFSEQLQNRLGDFASRLLEIRNCMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KYSDEDAEGIIQWISELFQAKPKTHSFAETRKIKKNLEALFVSVSIDSPYDLADRIVDLG
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHCC
VSDFILENTNFVVKLKNTPILSIILNELYSLRSIESIRLAVERTSKIVYSLKNYGRMDRG
CHHHEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SAKIQINIIDTIETVLTLYQNKMKSGIECIRLYNANPMIMGYPDELIQIWTNLIYNALQA
CCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHH
MHFKGKLTIQVEETGTDVEVSIKDDGPGIPLAVQRRIFEPFFTTKEKGEGTGLGLGIVKQ
HEECCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SVEERHKGQIQLFSEPGQTVFLVSLPKL
HHHHHCCCCEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]