Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 183221317

GI number: 183221317

Start: 2021221

End: 2022495

Strand: Reverse

Name: purA [H]

Synonym: LEPBI_I1934

Alternate gene names: 183221317

Gene position: 2022495-2021221 (Counterclockwise)

Preceding gene: 183221318

Following gene: 183221314

Centisome position: 56.19

GC content: 39.53

Gene sequence:

>1275_bases
ATGCCTGCAAATTTAGTCGTTGGAGCCCAGTGGGGTGATGAAGGAAAGGCAAAAGTAATTGATTACCTTTCAAAAGATAC
AGATATCATTGTACGTTACCAAGGTGGGGCTAATGCCGGTCACACGGTTGTTGTTGGTGGAAAAAAGTATATCTTCCATT
TGGTTCCATCTGGAATCATTTATGACAATACAACTTGTGTGATTGGGAATGGTGTGGTTCTCGATCCTGAGTATTTTTTG
AAAGAATGTGCGGACCTAGAGACACATGGCTTTCGTGTGAAAGACAAGGTTCTCATCAGTGATTCCTGCCATATCCTACT
TCCTTACCACAGATTGATCGATGAAGCAAGAGAAGCAGGTTCTTCTCCTGAACGTAAAATTGGTACCACGAAAAAAGGGA
TTGGTATGTGTTATGCGGATAAAATGCTTCGGAATGGAGTTCGCGCCGGTGATCTACTTGATAAGGAAAATTTAAAACGA
AAATTGAATCATATCTTGGAAGTAAAAAACCAAGAGCTTGTCAAATACTACGATTTGGAACCAGTGAATCCAAATGAAAT
GTATGACTTCTTATTGGATTTTGCTGATAAAATGGGAAAAAACATCATTAATACTGTGTATTACCTCAATTCTGAATTGG
AAAAAGGCAAAAGGGTATTACTAGAAGGTGCCCAAGGTACTGGACTTGATATTGATTTTGGAACCTATCCCTATGTAACA
AGCTCCAATCCTACCACGGGTGGTGCACTTGCTGGTTCAGGTGTAAGTTTTCGGTATTTGAAAGATGTGATTGGGATTAC
AAAAGCTTATGCCACGCGAGTTGGGGAAGGTCCTTTCCCTTCTGAAATTTTAGGTGAGGCTGGCGATGTTTTACGAAAGT
TAGGTGGTGAATATGGATCCACAACGGGTAGACCAAGACGTTGCGGTTGGTTTGATGTACAGATGATCAAACATGCAGTG
ACAGTAAATGGTATCAATTCACTAGTTTTAACAAAAATAGATGTACTCAGTCATTATGAAACGATCCCAGTCGTTGTTGG
ATATGAGTATAAAGGTAAAAAATTGGATTTCTTTCCATCACAAGGACTTGAAGACGTTAAACCATTGTTCGCTGAATATA
AAGGTTGGAAAGATGATATCTCTGGAATCAATTCTTTTGCAAAACTTCCTCCGCTGTGTCAGTCATATATTAAGTCCTTA
CAAGAGTTAGTGAATACAAAAATTGGGATCGTATCGACTGGACCCGATCGTGATCACACGATCATCATGGACTAA

Upstream 100 bases:

>100_bases
TTATATGCAAGTTTTACTGGAATTCAAAAGGATGCATGTGGTTTTGCACTGCATTTGGATGCAATCGAAGTTATGTTAAA
TAAGGAAAAATGAGGGATTT

Downstream 100 bases:

>100_bases
AAAAAAAAGGGGAGTTCACGATCTTTTTTTTAAGTAGTTGTTGACCGAGAAAGGTGGTTCGATATTCTTGGCTACAGAAC
GAGTCGTTAGCTCAGCTGGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPANLVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVVVGGKKYIFHLVPSGIIYDNTTCVIGNGVVLDPEYFL
KECADLETHGFRVKDKVLISDSCHILLPYHRLIDEAREAGSSPERKIGTTKKGIGMCYADKMLRNGVRAGDLLDKENLKR
KLNHILEVKNQELVKYYDLEPVNPNEMYDFLLDFADKMGKNIINTVYYLNSELEKGKRVLLEGAQGTGLDIDFGTYPYVT
SSNPTTGGALAGSGVSFRYLKDVIGITKAYATRVGEGPFPSEILGEAGDVLRKLGGEYGSTTGRPRRCGWFDVQMIKHAV
TVNGINSLVLTKIDVLSHYETIPVVVGYEYKGKKLDFFPSQGLEDVKPLFAEYKGWKDDISGINSFAKLPPLCQSYIKSL
QELVNTKIGIVSTGPDRDHTIIMD

Sequences:

>Translated_424_residues
MPANLVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVVVGGKKYIFHLVPSGIIYDNTTCVIGNGVVLDPEYFL
KECADLETHGFRVKDKVLISDSCHILLPYHRLIDEAREAGSSPERKIGTTKKGIGMCYADKMLRNGVRAGDLLDKENLKR
KLNHILEVKNQELVKYYDLEPVNPNEMYDFLLDFADKMGKNIINTVYYLNSELEKGKRVLLEGAQGTGLDIDFGTYPYVT
SSNPTTGGALAGSGVSFRYLKDVIGITKAYATRVGEGPFPSEILGEAGDVLRKLGGEYGSTTGRPRRCGWFDVQMIKHAV
TVNGINSLVLTKIDVLSHYETIPVVVGYEYKGKKLDFFPSQGLEDVKPLFAEYKGWKDDISGINSFAKLPPLCQSYIKSL
QELVNTKIGIVSTGPDRDHTIIMD
>Mature_423_residues
PANLVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVVVGGKKYIFHLVPSGIIYDNTTCVIGNGVVLDPEYFLK
ECADLETHGFRVKDKVLISDSCHILLPYHRLIDEAREAGSSPERKIGTTKKGIGMCYADKMLRNGVRAGDLLDKENLKRK
LNHILEVKNQELVKYYDLEPVNPNEMYDFLLDFADKMGKNIINTVYYLNSELEKGKRVLLEGAQGTGLDIDFGTYPYVTS
SNPTTGGALAGSGVSFRYLKDVIGITKAYATRVGEGPFPSEILGEAGDVLRKLGGEYGSTTGRPRRCGWFDVQMIKHAVT
VNGINSLVLTKIDVLSHYETIPVVVGYEYKGKKLDFFPSQGLEDVKPLFAEYKGWKDDISGINSFAKLPPLCQSYIKSLQ
ELVNTKIGIVSTGPDRDHTIIMD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=40.7058823529412, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=39.4366197183099, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=38.5390428211587, Blast_Score=268, Evalue=1e-71,
Organism=Escherichia coli, GI1790620, Length=422, Percent_Identity=44.3127962085308, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=40.3263403263403, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=40.3263403263403, Blast_Score=320, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=40.4109589041096, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=41.1347517730496, Blast_Score=319, Evalue=2e-87,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46806; Mature: 46675

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANLVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVVVGGKKYIFHLVPSGII
CCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEEECCCCEE
YDNTTCVIGNGVVLDPEYFLKECADLETHGFRVKDKVLISDSCHILLPYHRLIDEAREAG
ECCCEEEECCCEEECHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHCC
SSPERKIGTTKKGIGMCYADKMLRNGVRAGDLLDKENLKRKLNHILEVKNQELVKYYDLE
CCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PVNPNEMYDFLLDFADKMGKNIINTVYYLNSELEKGKRVLLEGAQGTGLDIDFGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCEEECCCCCEEE
SSNPTTGGALAGSGVSFRYLKDVIGITKAYATRVGEGPFPSEILGEAGDVLRKLGGEYGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
TTGRPRRCGWFDVQMIKHAVTVNGINSLVLTKIDVLSHYETIPVVVGYEYKGKKLDFFPS
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCC
QGLEDVKPLFAEYKGWKDDISGINSFAKLPPLCQSYIKSLQELVNTKIGIVSTGPDRDHT
CCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
IIMD
EEEC
>Mature Secondary Structure 
PANLVVGAQWGDEGKAKVIDYLSKDTDIIVRYQGGANAGHTVVVGGKKYIFHLVPSGII
CCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEEECCCCEE
YDNTTCVIGNGVVLDPEYFLKECADLETHGFRVKDKVLISDSCHILLPYHRLIDEAREAG
ECCCEEEECCCEEECHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHCC
SSPERKIGTTKKGIGMCYADKMLRNGVRAGDLLDKENLKRKLNHILEVKNQELVKYYDLE
CCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
PVNPNEMYDFLLDFADKMGKNIINTVYYLNSELEKGKRVLLEGAQGTGLDIDFGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCEEECCCCCEEE
SSNPTTGGALAGSGVSFRYLKDVIGITKAYATRVGEGPFPSEILGEAGDVLRKLGGEYGS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
TTGRPRRCGWFDVQMIKHAVTVNGINSLVLTKIDVLSHYETIPVVVGYEYKGKKLDFFPS
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCC
QGLEDVKPLFAEYKGWKDDISGINSFAKLPPLCQSYIKSLQELVNTKIGIVSTGPDRDHT
CCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
IIMD
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA