Click here to switch to the map view.

The map label for this gene is bedB [H]

Identifier: 183221301

GI number: 183221301

Start: 2007486

End: 2007803

Strand: Reverse

Name: bedB [H]

Synonym: LEPBI_I1918

Alternate gene names: 183221301

Gene position: 2007803-2007486 (Counterclockwise)

Preceding gene: 183221302

Following gene: 183221300

Centisome position: 55.78

GC content: 40.25

Gene sequence:

>318_bases
ATGATGGCGTTTAAAAAATTAATTTCCATTCAGGAAATTGAAGAAGGGAAACTTTCTGTCATCAAAACTCGGCATTTTTC
CGTTGTTGTCACCAAATGGCAAAATCAATATTTTGCATTTGAAGATTCCTGTACCCATGATGGGGAAGAAATTTCCTGTG
GAAAATTAGAAGGATGTGTGATCACTTGCCCCAGGCACTTTGCCAAATTTGACATTCGTGATGGAAGTGTACTGGCATTG
CCCGCGACAGAACCATTGACGATGTTTCCTACAAAAGTGAATGGGGATGATTTGGAAGTGGATTTGGAGTCCGTATGA

Upstream 100 bases:

>100_bases
TCACTGCATTCTACGGGGAAACCATCCAACGAATTGGTTTTTCGGATCAGATCAAAGAAGGATTGGAATCTACCATTCAT
GGTTTACTTGTGGGAGGGAA

Downstream 100 bases:

>100_bases
GTTTAGATCCATACAATTTACGCAAAGATTTCCCAATCCTTTCTCAAACCATGCCCAATGGGAAAGAACTTGTTTATTTA
GACAATGGAGCTACTTCCCA

Product: reductase/dioxygenase

Products: cis-cyclohexa-3,5-diene-1,2-diol; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MMAFKKLISIQEIEEGKLSVIKTRHFSVVVTKWQNQYFAFEDSCTHDGEEISCGKLEGCVITCPRHFAKFDIRDGSVLAL
PATEPLTMFPTKVNGDDLEVDLESV

Sequences:

>Translated_105_residues
MMAFKKLISIQEIEEGKLSVIKTRHFSVVVTKWQNQYFAFEDSCTHDGEEISCGKLEGCVITCPRHFAKFDIRDGSVLAL
PATEPLTMFPTKVNGDDLEVDLESV
>Mature_105_residues
MMAFKKLISIQEIEEGKLSVIKTRHFSVVVTKWQNQYFAFEDSCTHDGEEISCGKLEGCVITCPRHFAKFDIRDGSVLAL
PATEPLTMFPTKVNGDDLEVDLESV

Specific function: This protein seems to be a 2Fe-2S ferredoxin [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788890, Length=94, Percent_Identity=34.0425531914894, Blast_Score=62, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.12.3

Molecular weight: Translated: 11812; Mature: 11812

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAFKKLISIQEIEEGKLSVIKTRHFSVVVTKWQNQYFAFEDSCTHDGEEISCGKLEGCV
CCCHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCEEEEECCCCCCCCCEECCCCCCCEE
ITCPRHFAKFDIRDGSVLALPATEPLTMFPTKVNGDDLEVDLESV
EECCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCEEEEEECC
>Mature Secondary Structure
MMAFKKLISIQEIEEGKLSVIKTRHFSVVVTKWQNQYFAFEDSCTHDGEEISCGKLEGCV
CCCHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCEEEEECCCCCCCCCEECCCCCCCEE
ITCPRHFAKFDIRDGSVLALPATEPLTMFPTKVNGDDLEVDLESV
EECCCCCEEEECCCCCEEEECCCCCEEECCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzene; NADH; H+; O2

Specific reaction: benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344526 [H]