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The map label for this gene is rlmI [H]

Identifier: 183221205

GI number: 183221205

Start: 1900963

End: 1902144

Strand: Reverse

Name: rlmI [H]

Synonym: LEPBI_I1819

Alternate gene names: 183221205

Gene position: 1902144-1900963 (Counterclockwise)

Preceding gene: 183221207

Following gene: 183221204

Centisome position: 52.84

GC content: 35.36

Gene sequence:

>1182_bases
ATGGTCATTCGTTTAAAGAAGAATAAAGAAAAAGCAGTATTAAATTTTCATCCTTGGATCTTTTCCGGTGCCATAGAATC
CGTCGACAATGGAATCAAATCTGGTGATACCGTTGCCGTGCACAGTCATGACGGTACATTTTTGGCTTGGGGTCATTATG
ATTCCGGAAGCCAAATTCGAATCAGATTGTTCTCTTTTGATCCAAAACAAGATGGAATCAATCGCGATTTCTGGTTCTCG
AAATGGGAAAAGATTCTATATACCAAAAGGTCACTATTACCGAACGATACCAATGGATTCCGTTTGTTTCATTCGGAAGG
GGATGGAGTTCCAGGCATTGTAGTGGATGTTTACGATGATACTGCCGTTCTACAATTAAAAACACCTGGCGCCATTCGCT
TAAAAGAAAATTTAGTTACGTTCTTGGAAAAATTTGGTTTTGTTACCATTTTAGAAAAAAGAGAAAAAACTGATTCGAAA
CATGGAGATGAAATCAGCTTTTATAAAGGAGATAAAGCAGAGACCGTTTTTAAAGAAAATGGAATTCAATTTATATCTGA
TACTACGAAAGGACAAAAAACTGGATTTTTTTTGGACCAAAGGGAAAATCGATCGCTCTTAGGTAGATACGCAAAAAACA
AAATCATTTTAAATACATTTGCCTACTCAGGTGCATTTTCAGTGTATGCATTGTTAAATGGCGCAAAGATGGTTCATAGC
TTAGATATTTCCAAACAAGCATTGGAATTATGTGAAAAAAATTTAGAACTTAATGGTTTCTCACAGGAACTTTTGGCGGT
AAAACATAAATCTCTCGTGATGGATTGTTTTGATTATTTAAAAAAAATGGATTCTGGAATTTATGACTGCATCATTCTTG
ATCCACCTGCCTTTACAAAAAATATTGCTACGGTCATACAAGCAAGTAGGGGGTATAAAGACATTAATATGAGAGCCATT
TCCAAAATACAAGATGGTGGACTTATCTTTACCTTCTCCTGTTCCCAACATATTTCCTTTGATTTATTCAAAAAAATCGT
TTTTGGTGCGGCAAAAGATGCGAAAAAAAAAGTTCGAATTCTGCACCATTTAACACAAAGTCCTGACCATAGTTATTCTG
TTTTCCATCCGGAAGGAGAATACTTAAAAGGACTTGTGATTCAGGTTGATGGACAAATATAA

Upstream 100 bases:

>100_bases
GTTGGGAAAAAGAAAAGACAAGAAAAATCAGTAAGTTTTTAATCATATTTCAGTGGGTTATGTTTGCCCAAAACAATTCG
TAAGTGCAATTGGATGAAGA

Downstream 100 bases:

>100_bases
ATGAAAATACTGGTGAAATCTTAGAATGAAATTTACATCGCTCAGTTTCCTTTTGTTCTTTCTCGTCGTGTATGGATTGC
ATTGGATGATCCGTGGCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVIRLKKNKEKAVLNFHPWIFSGAIESVDNGIKSGDTVAVHSHDGTFLAWGHYDSGSQIRIRLFSFDPKQDGINRDFWFS
KWEKILYTKRSLLPNDTNGFRLFHSEGDGVPGIVVDVYDDTAVLQLKTPGAIRLKENLVTFLEKFGFVTILEKREKTDSK
HGDEISFYKGDKAETVFKENGIQFISDTTKGQKTGFFLDQRENRSLLGRYAKNKIILNTFAYSGAFSVYALLNGAKMVHS
LDISKQALELCEKNLELNGFSQELLAVKHKSLVMDCFDYLKKMDSGIYDCIILDPPAFTKNIATVIQASRGYKDINMRAI
SKIQDGGLIFTFSCSQHISFDLFKKIVFGAAKDAKKKVRILHHLTQSPDHSYSVFHPEGEYLKGLVIQVDGQI

Sequences:

>Translated_393_residues
MVIRLKKNKEKAVLNFHPWIFSGAIESVDNGIKSGDTVAVHSHDGTFLAWGHYDSGSQIRIRLFSFDPKQDGINRDFWFS
KWEKILYTKRSLLPNDTNGFRLFHSEGDGVPGIVVDVYDDTAVLQLKTPGAIRLKENLVTFLEKFGFVTILEKREKTDSK
HGDEISFYKGDKAETVFKENGIQFISDTTKGQKTGFFLDQRENRSLLGRYAKNKIILNTFAYSGAFSVYALLNGAKMVHS
LDISKQALELCEKNLELNGFSQELLAVKHKSLVMDCFDYLKKMDSGIYDCIILDPPAFTKNIATVIQASRGYKDINMRAI
SKIQDGGLIFTFSCSQHISFDLFKKIVFGAAKDAKKKVRILHHLTQSPDHSYSVFHPEGEYLKGLVIQVDGQI
>Mature_393_residues
MVIRLKKNKEKAVLNFHPWIFSGAIESVDNGIKSGDTVAVHSHDGTFLAWGHYDSGSQIRIRLFSFDPKQDGINRDFWFS
KWEKILYTKRSLLPNDTNGFRLFHSEGDGVPGIVVDVYDDTAVLQLKTPGAIRLKENLVTFLEKFGFVTILEKREKTDSK
HGDEISFYKGDKAETVFKENGIQFISDTTKGQKTGFFLDQRENRSLLGRYAKNKIILNTFAYSGAFSVYALLNGAKMVHS
LDISKQALELCEKNLELNGFSQELLAVKHKSLVMDCFDYLKKMDSGIYDCIILDPPAFTKNIATVIQASRGYKDINMRAI
SKIQDGGLIFTFSCSQHISFDLFKKIVFGAAKDAKKKVRILHHLTQSPDHSYSVFHPEGEYLKGLVIQVDGQI

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=399, Percent_Identity=36.0902255639098, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1787181, Length=215, Percent_Identity=29.7674418604651, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44445; Mature: 44445

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIRLKKNKEKAVLNFHPWIFSGAIESVDNGIKSGDTVAVHSHDGTFLAWGHYDSGSQIR
CEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCEEE
IRLFSFDPKQDGINRDFWFSKWEKILYTKRSLLPNDTNGFRLFHSEGDGVPGIVVDVYDD
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECC
TAVLQLKTPGAIRLKENLVTFLEKFGFVTILEKREKTDSKHGDEISFYKGDKAETVFKEN
EEEEEEECCCCEEEHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCCHHHHHHC
GIQFISDTTKGQKTGFFLDQRENRSLLGRYAKNKIILNTFAYSGAFSVYALLNGAKMVHS
CCEEEECCCCCCCCCEEEECCCCHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHHHE
LDISKQALELCEKNLELNGFSQELLAVKHKSLVMDCFDYLKKMDSGIYDCIILDPPAFTK
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
NIATVIQASRGYKDINMRAISKIQDGGLIFTFSCSQHISFDLFKKIVFGAAKDAKKKVRI
HHHHHHHHCCCCCCCCHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
LHHLTQSPDHSYSVFHPEGEYLKGLVIQVDGQI
HHHHHCCCCCCEEEECCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MVIRLKKNKEKAVLNFHPWIFSGAIESVDNGIKSGDTVAVHSHDGTFLAWGHYDSGSQIR
CEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCCEEE
IRLFSFDPKQDGINRDFWFSKWEKILYTKRSLLPNDTNGFRLFHSEGDGVPGIVVDVYDD
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECC
TAVLQLKTPGAIRLKENLVTFLEKFGFVTILEKREKTDSKHGDEISFYKGDKAETVFKEN
EEEEEEECCCCEEEHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCCHHHHHHC
GIQFISDTTKGQKTGFFLDQRENRSLLGRYAKNKIILNTFAYSGAFSVYALLNGAKMVHS
CCEEEECCCCCCCCCEEEECCCCHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHHHE
LDISKQALELCEKNLELNGFSQELLAVKHKSLVMDCFDYLKKMDSGIYDCIILDPPAFTK
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
NIATVIQASRGYKDINMRAISKIQDGGLIFTFSCSQHISFDLFKKIVFGAAKDAKKKVRI
HHHHHHHHCCCCCCCCHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
LHHLTQSPDHSYSVFHPEGEYLKGLVIQVDGQI
HHHHHCCCCCCEEEECCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA