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The map label for this gene is miaB

Identifier: 183221201

GI number: 183221201

Start: 1896352

End: 1897728

Strand: Reverse

Name: miaB

Synonym: LEPBI_I1815

Alternate gene names: 183221201

Gene position: 1897728-1896352 (Counterclockwise)

Preceding gene: 183221203

Following gene: 183221200

Centisome position: 52.72

GC content: 37.84

Gene sequence:

>1377_bases
ATGAACCCTACCTTGACAGAAAGCCAAACACTTACCCCCACCATCCAATTGGGAAAGGTCTATGTGGAGACCTACGGCTG
CCAAATGAACGAATATGACTCAGGCATTGTTAAGGAACTCTTTCGAAAGGAACATTATGAAACCACAAATGTAGTCGAAG
AAAGTGATATTATTTTCTTAAATACTTGTGCTGTCAGAGAAAATGCCCATGCAAAAATCTACGGAAGATTACAATCCTTA
GGTTATCTCAAGAAAAAAAATCCAAACCTTGTGATTGGTGTACTTGGTTGTATGGCACAAAACTTGGGAGAAGATTTATT
CCACCAAGAGTTACCCTTGGATTTGATCGTTGGCCCAGATAATTATCGAACACTACCGGAGCTCATTCAAAACATCCGAA
ATGGGGAAAAAGATGTACAACTCACAAGGTTATCAAGATCAGAAACCTATGATGAATTAGAACCGAAGGTAGTCAATGGA
ATCCAAGCCTTCGTTACCATTATGCGCGGTTGTAATAATTTTTGTACGTTTTGTGTGGTTCCCTATACGAGAGGGAGAGA
GAGGAGTCGAGAACCAAAATCCATCATCCACGAAATCAAACAATTACAAGAGATGGGTGTCAAACAAGTCACCCTTCTTG
GACAAAACGTAAATAGTTACTCTTATGAGTCCTACGACTTTTGTGCCTTGGTGGAATCAATTCTCAAAGAAACCACCATT
GAAAGAGTGAGATTCACAAGTCCACACCCCAAAGATTTTCCTGACCATCTAATTTCTCTTATGGCAAAAGAGGATCGTTT
TTCTTCACAAATTCACATGCCACTGCAAGCAGGTAGTTCGAAAGTCCTTAGAGATATGAAACGTAGTTATACTAAGGAAG
AGTATTTGGATTTAGTAAAGAAAATCCAATCTGTGATTCCTGACATCGGGATCACATCTGATATCATCGTTGGGTTTCCA
GGGGAAACAGAAGAAGAATTTTTGGAAACTTTGGAAGTTGTGAAAAAAGTGAAATTTGACATGTCTTATATGTTCAAATA
TTCAGAAAGAGAAGGAACGATCGCAAAACGGAAGTTTATTGATGATGTTCCAGAAGAAGTAAAAAGTCGCCGACTTATCG
AACTTGTTGAATTACAAACAAAAATCTCTTTAGAAAAAAATACTTCTAAGATTGGTAAGATCTTTTCCATTTTGATAGAA
AATACTTCCAAAAAATCAAAACAAGAGTTATGTGGTAGATCACATTGCGGTCGAATGGTTGTTTTTCCAATTCCAGAAGG
AATGTCCCAGGATTTATCCGATTGGATAGGGAAAACAGTAAATGTTTTGATCGAACAAGCCACAAGTGCTACACTCAAAG
GGAAATTGATTGTCTAA

Upstream 100 bases:

>100_bases
AGAATTTCGTAATAGGTTTCCCTTTTGGTTCTCGGCATATACCCTTTACGCCCCTTCCTTCTTTGCTTTACAAGGAATTT
TCGCCGGAAAATATATCATT

Downstream 100 bases:

>100_bases
ACCAGTTGACCTAAGAACCGTTAGGGTTCATTCCAAAGATGATTTGCCACCAGGTTTTATGGTGGATACTGATCGTTTAG
GAAAATGGAAACGGTTCAGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFLNTCAVRENAHAKIYGRLQSL
GYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPDNYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNG
IQAFVTIMRGCNNFCTFCVVPYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI
ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVKKIQSVIPDIGITSDIIVGFP
GETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFIDDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIE
NTSKKSKQELCGRSHCGRMVVFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV

Sequences:

>Translated_458_residues
MNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFLNTCAVRENAHAKIYGRLQSL
GYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPDNYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNG
IQAFVTIMRGCNNFCTFCVVPYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI
ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVKKIQSVIPDIGITSDIIVGFP
GETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFIDDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIE
NTSKKSKQELCGRSHCGRMVVFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV
>Mature_458_residues
MNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFLNTCAVRENAHAKIYGRLQSL
GYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPDNYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNG
IQAFVTIMRGCNNFCTFCVVPYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI
ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVKKIQSVIPDIGITSDIIVGFP
GETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFIDDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIE
NTSKKSKQELCGRSHCGRMVVFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=40.3765690376569, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=40.3765690376569, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI93277076, Length=464, Percent_Identity=25.4310344827586, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=42.0224719101124, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1787057, Length=418, Percent_Identity=29.4258373205742, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=38.494623655914, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI71996771, Length=329, Percent_Identity=26.4437689969605, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356207, Length=473, Percent_Identity=39.9577167019027, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI19922432, Length=469, Percent_Identity=25.7995735607676, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_LEPBA (B0S9E2)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962847.1
- ProteinModelPortal:   B0S9E2
- SMR:   B0S9E2
- GeneID:   6388290
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1762
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14332
- BioCyc:   LBIF355278:LBF_1762-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52295; Mature: 52295

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFL
CCCCCCCCCCCCCHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCEEEE
NTCAVRENAHAKIYGRLQSLGYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPD
EEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCC
NYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNGIQAFVTIMRGCNNFCTFCVV
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEE
PYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVK
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCHHCCCHHHHHHHHHHCCHHHHHHHHH
KIQSVIPDIGITSDIIVGFPGETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFI
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIENTSKKSKQELCGRSHCGRMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFL
CCCCCCCCCCCCCHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCEEEE
NTCAVRENAHAKIYGRLQSLGYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPD
EEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEECCC
NYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNGIQAFVTIMRGCNNFCTFCVV
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEE
PYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVK
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCHHCCCHHHHHHHHHHCCHHHHHHHHH
KIQSVIPDIGITSDIIVGFPGETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFI
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIENTSKKSKQELCGRSHCGRMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA