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The map label for this gene is amtB [C]

Identifier: 183221153

GI number: 183221153

Start: 1844400

End: 1845827

Strand: Reverse

Name: amtB [C]

Synonym: LEPBI_I1767

Alternate gene names: 183221153

Gene position: 1845827-1844400 (Counterclockwise)

Preceding gene: 183221154

Following gene: 183221152

Centisome position: 51.28

GC content: 46.5

Gene sequence:

>1428_bases
ATGAAACATTTCGGAATCTATTTTACATTGATGGCCCTATTTTTGGTCAGCGTGCTGAACGCTGAGTCTCTTGAGAACGG
GGAAAAGGAAAGTTTGGAATCCTTGGCAAAAGACTTGGCTGTTATCAAGGCCTCTCTTGCAGAAACAAAACTTGCCCTCG
AAACCACAAAGCAAGAAGCCAATTGGGTTTGGACTTGTATTGCCGCCTTTTTGGTTTTTTTTATGCAAGCTGGGTTTGCG
TATGTCGAAGCAGGATTTACGAGAGCCAAAAATGCAGTGAACATCCTTATGAAAAACTTTTCCGACTTAACAGTAGGTGC
CCTCGCGTATTGGCTCATTGGTTTTTCGATTATGTTTGGCCCACAAGTTTTGGATGGATTTGGAATTGGAGTCCCCTCGT
TTGCAGAATCTCTCATCAATGCAGAAGATGGGACTATGGATCCAGGTAAGTACACTTTCTTTATCTTCCAAATTGTATTT
GCTGCCACTGCCGCCACGATTGTTTCGGGTGCGATGGCAGAACGAACCAAGTTTTCTGCTTACCTGATTTTTTCTGTAAT
CATCACTGCCGTCATTTACCCTGTTTTTGGATCTTTCGCTTGGGGGAGTTTACTCGGAGTCTCCACAGGATTTTTAGAGT
CCCTCGGACTTGGCGGAGGTGAAGGTGTTGGGTTTCATGATTTTGCTGGATCGACAGTCGTACATAGCATCGGTGCCTGG
GCTGGACTTGCGGGAGCAATAGTTGTTGGTCCAAGGATGGGCAAATTCCAATCCGATGGTAGGGTCTATCCAATCCTTGG
GCATAATATGTCTATGGCAGCCCTTGGTGTGTTTATCTTATGGTTTGGTTGGTTTGGATTTAACCCAGGGTCTACCACTT
CCATCGAAGGGGGGAGTTTTGCCAAGATCGCAGTCGTGACTCATATGGCAGCTTGTGCAGGTGCTCTCAGTGCTATGGCA
CTCACTTGGTATCTATTCAAAAAACCGGAGATTGGACTTACCCTCAATGGAGGCCTTGCCGGGCTTGTTGCCATCACGGC
ACCATGTGATAATGTGAGCATTTTGGGCTCAGTGATCATTGGCCTTGTGGCTGGTATTTTAGTCATTGTTTCGGTTCTCT
TTTTGGACAATCGCAAAATCGATGATCCCGTAGGTGCTGTCTCCGTGCATGGGGTCTGCGGCGCATGGGGGACAATGGCT
TATGGGTTGTTTAGTTTGGACACGGGACTCTTTTACGGCGCGGGATTTGCTCAATTTGCGGCCCAAGCCATTGGAGTGGT
CACTGCATTTTTATGGGCTTTCCCTGCGAGTTTACTCATGTTCTATCTCATCAAAAAAACAGTCGGGCTTCGCGTTTCGG
AAGAAGAAGAGCTGTTAGGTTTGGATATTTTAGAACACGGAAACGAAGCCTATCCAGTTTCCAAATAG

Upstream 100 bases:

>100_bases
TTACAACAAGGCGTAAAAAACACCCCTCAAAGTTTGGAATTAAAAACCTCTCTTTTGAGATTAGAATCACATACACAAAA
AGTGAGTTAAGGAAAGTTAT

Downstream 100 bases:

>100_bases
TCCTTGACCCTAGATTTCTCCGTGCGGAAGTCTAGGGTGTGTTTCGAATTTTATTATGTTTACTCCCTTTTTGTTATGGT
TGCTTTGAATACGAAGAAAC

Product: ammonium ABC transporter permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKHFGIYFTLMALFLVSVLNAESLENGEKESLESLAKDLAVIKASLAETKLALETTKQEANWVWTCIAAFLVFFMQAGFA
YVEAGFTRAKNAVNILMKNFSDLTVGALAYWLIGFSIMFGPQVLDGFGIGVPSFAESLINAEDGTMDPGKYTFFIFQIVF
AATAATIVSGAMAERTKFSAYLIFSVIITAVIYPVFGSFAWGSLLGVSTGFLESLGLGGGEGVGFHDFAGSTVVHSIGAW
AGLAGAIVVGPRMGKFQSDGRVYPILGHNMSMAALGVFILWFGWFGFNPGSTTSIEGGSFAKIAVVTHMAACAGALSAMA
LTWYLFKKPEIGLTLNGGLAGLVAITAPCDNVSILGSVIIGLVAGILVIVSVLFLDNRKIDDPVGAVSVHGVCGAWGTMA
YGLFSLDTGLFYGAGFAQFAAQAIGVVTAFLWAFPASLLMFYLIKKTVGLRVSEEEELLGLDILEHGNEAYPVSK

Sequences:

>Translated_475_residues
MKHFGIYFTLMALFLVSVLNAESLENGEKESLESLAKDLAVIKASLAETKLALETTKQEANWVWTCIAAFLVFFMQAGFA
YVEAGFTRAKNAVNILMKNFSDLTVGALAYWLIGFSIMFGPQVLDGFGIGVPSFAESLINAEDGTMDPGKYTFFIFQIVF
AATAATIVSGAMAERTKFSAYLIFSVIITAVIYPVFGSFAWGSLLGVSTGFLESLGLGGGEGVGFHDFAGSTVVHSIGAW
AGLAGAIVVGPRMGKFQSDGRVYPILGHNMSMAALGVFILWFGWFGFNPGSTTSIEGGSFAKIAVVTHMAACAGALSAMA
LTWYLFKKPEIGLTLNGGLAGLVAITAPCDNVSILGSVIIGLVAGILVIVSVLFLDNRKIDDPVGAVSVHGVCGAWGTMA
YGLFSLDTGLFYGAGFAQFAAQAIGVVTAFLWAFPASLLMFYLIKKTVGLRVSEEEELLGLDILEHGNEAYPVSK
>Mature_475_residues
MKHFGIYFTLMALFLVSVLNAESLENGEKESLESLAKDLAVIKASLAETKLALETTKQEANWVWTCIAAFLVFFMQAGFA
YVEAGFTRAKNAVNILMKNFSDLTVGALAYWLIGFSIMFGPQVLDGFGIGVPSFAESLINAEDGTMDPGKYTFFIFQIVF
AATAATIVSGAMAERTKFSAYLIFSVIITAVIYPVFGSFAWGSLLGVSTGFLESLGLGGGEGVGFHDFAGSTVVHSIGAW
AGLAGAIVVGPRMGKFQSDGRVYPILGHNMSMAALGVFILWFGWFGFNPGSTTSIEGGSFAKIAVVTHMAACAGALSAMA
LTWYLFKKPEIGLTLNGGLAGLVAITAPCDNVSILGSVIIGLVAGILVIVSVLFLDNRKIDDPVGAVSVHGVCGAWGTMA
YGLFSLDTGLFYGAGFAQFAAQAIGVVTAFLWAFPASLLMFYLIKKTVGLRVSEEEELLGLDILEHGNEAYPVSK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=451, Percent_Identity=32.3725055432373, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=456, Percent_Identity=35.5263157894737, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=34.9557522123894, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI32564042, Length=449, Percent_Identity=32.5167037861915, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI133931100, Length=446, Percent_Identity=29.8206278026906, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=443, Percent_Identity=25.9593679458239, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=433, Percent_Identity=28.175519630485, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=26.8691588785047, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078314, Length=428, Percent_Identity=36.6822429906542, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50244; Mature: 50244

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFGIYFTLMALFLVSVLNAESLENGEKESLESLAKDLAVIKASLAETKLALETTKQEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NWVWTCIAAFLVFFMQAGFAYVEAGFTRAKNAVNILMKNFSDLTVGALAYWLIGFSIMFG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
PQVLDGFGIGVPSFAESLINAEDGTMDPGKYTFFIFQIVFAATAATIVSGAMAERTKFSA
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLIFSVIITAVIYPVFGSFAWGSLLGVSTGFLESLGLGGGEGVGFHDFAGSTVVHSIGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHH
AGLAGAIVVGPRMGKFQSDGRVYPILGHNMSMAALGVFILWFGWFGFNPGSTTSIEGGSF
HHHCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AKIAVVTHMAACAGALSAMALTWYLFKKPEIGLTLNGGLAGLVAITAPCDNVSILGSVII
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCEEEEEEECCCCCHHHHHHHHH
GLVAGILVIVSVLFLDNRKIDDPVGAVSVHGVCGAWGTMAYGLFSLDTGLFYGAGFAQFA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHCCCHHHHH
AQAIGVVTAFLWAFPASLLMFYLIKKTVGLRVSEEEELLGLDILEHGNEAYPVSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKHFGIYFTLMALFLVSVLNAESLENGEKESLESLAKDLAVIKASLAETKLALETTKQEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NWVWTCIAAFLVFFMQAGFAYVEAGFTRAKNAVNILMKNFSDLTVGALAYWLIGFSIMFG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
PQVLDGFGIGVPSFAESLINAEDGTMDPGKYTFFIFQIVFAATAATIVSGAMAERTKFSA
HHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLIFSVIITAVIYPVFGSFAWGSLLGVSTGFLESLGLGGGEGVGFHDFAGSTVVHSIGAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHH
AGLAGAIVVGPRMGKFQSDGRVYPILGHNMSMAALGVFILWFGWFGFNPGSTTSIEGGSF
HHHCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AKIAVVTHMAACAGALSAMALTWYLFKKPEIGLTLNGGLAGLVAITAPCDNVSILGSVII
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCCEEEEEEECCCCCHHHHHHHHH
GLVAGILVIVSVLFLDNRKIDDPVGAVSVHGVCGAWGTMAYGLFSLDTGLFYGAGFAQFA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHCCCHHHHH
AQAIGVVTAFLWAFPASLLMFYLIKKTVGLRVSEEEELLGLDILEHGNEAYPVSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]