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The map label for this gene is ntrX [H]

Identifier: 183221041

GI number: 183221041

Start: 1729550

End: 1730902

Strand: Direct

Name: ntrX [H]

Synonym: LEPBI_I1654

Alternate gene names: 183221041

Gene position: 1729550-1730902 (Clockwise)

Preceding gene: 183221040

Following gene: 183221042

Centisome position: 48.05

GC content: 37.69

Gene sequence:

>1353_bases
ATGCAAAAATTAATCTATATTTTAGACGATGAAAAAGAAATCCGAAAAACGTTACGAGTGATTTTGGAAGATGAGGAATA
TTCTGTCGAAGATTTTTCGAGTGGGAAGTCATTGATAAAAGCATTAACCAAAGAGAGACCTTCACTTGTATTACTCGATG
TATGGGTTGGAAAAGAAGACGGACTGACCATTTTAGATGAATGCAAAAAGTTATATCCTAGTCTTCCCATTGTGATGATT
TCAGGTCACGGGACAATTGAACTTGCGGTCAATGCGACGAAAAAAGGTGCCGTGGATTTTTTAGAGAAACCATTATCCAT
CGAAAAAGTAATCCAAACGATCGAGTCTTCCATCGAAAAAACAAAAGAAGTTCATTTTCCCAATTTTAAATTAGATGTAG
ATGAAATCTTAGGTGTGTCTCCATCTATCACTCGAGTTAAGTTTGCTGTTTTCCAGGCCGCAGAAACGAATGCTCGTGTT
TTTATTTATGGTGAAAATGGCACTGGAAAAGAACTTACTGCAAGAGCCATCCATCAAAATTCGAAACGAAAAAATGAACC
TTACATTGAGTTCAATTGTGCGTCCTTACCAGAAGAGAGCTTAGAACAAGAGTTATTTGGTTTAGAACTTGCTTCACAGA
CTGATTCTCCTGAAATCCGAATTGGAAAATGGGAACAGGCTCAAAATGGTACTTTATTTTTAGATGAAATCTGTGATTTG
AGTTTGGCTCTCCAATCGAAAGTTCTAAAAGTCATTCTGGATCAAAAATTAGAACGAGTTGGGGGTAAGGAGTTCATTCC
CGTTGATGTTCGGATCATCGCCGCAACCAATTCCAATGTGGAAGATGCGATCCGTGAAGGAAAATTTAGAGAAGATCTAT
TTTATGCTTTAAATGTAATTCCTCTGGAATTACCACCGTTACGCGAACGTAACCAAGATATCCCACTGCTTGCCGAATAC
TATTTAAAAAAATCAATATCTGAAAATCATCTATCTCAAAAAACCATTGATCGAGACGGATTAGATGCACTTTCGTCCCA
TTTTTGGCCAGGAAATGTAAGGGAACTAACCAATATCATCGAACGATTGAGTATCTTAGTTCCCGGAGATACAATCAAAG
CGAAGGATGTCAAAGAAGCCCTACATGGGTTCAAAAGGGCAAACGAGATGGTGGCGAGGGGTGATTTAAAACATGCAAAA
GAAGAGTTTGAACGTCAGTACATTATCAAAACCTTACAAATTTGTGAGGGGAATGTCACTCGTACTTCCAAAGCATTAGG
AATCGAACGAACTCATTTATACAGAAAGTTAAGATCACTCAATATTTCTGTTGATCAATTGATAGAGGGTTAG

Upstream 100 bases:

>100_bases
TCCAAAAAACGATCATTGACCATCATGGGCATATTTCCGTTGAGAACTCAAAGTTAGGTGGCTGTAAGTTTCGTATTGAA
CTTCCTTTGGATCTTTCATA

Downstream 100 bases:

>100_bases
TATGGACCAAAAAACAATATTAGAGACATTTACCGATATTCTCAAAGAAACTAAATTTCTCATCCAAAATCATTCCATAT
CCATCTATCGATTTCAAGAC

Product: putative nitrogen assimilation regulatory protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MQKLIYILDDEKEIRKTLRVILEDEEYSVEDFSSGKSLIKALTKERPSLVLLDVWVGKEDGLTILDECKKLYPSLPIVMI
SGHGTIELAVNATKKGAVDFLEKPLSIEKVIQTIESSIEKTKEVHFPNFKLDVDEILGVSPSITRVKFAVFQAAETNARV
FIYGENGTGKELTARAIHQNSKRKNEPYIEFNCASLPEESLEQELFGLELASQTDSPEIRIGKWEQAQNGTLFLDEICDL
SLALQSKVLKVILDQKLERVGGKEFIPVDVRIIAATNSNVEDAIREGKFREDLFYALNVIPLELPPLRERNQDIPLLAEY
YLKKSISENHLSQKTIDRDGLDALSSHFWPGNVRELTNIIERLSILVPGDTIKAKDVKEALHGFKRANEMVARGDLKHAK
EEFERQYIIKTLQICEGNVTRTSKALGIERTHLYRKLRSLNISVDQLIEG

Sequences:

>Translated_450_residues
MQKLIYILDDEKEIRKTLRVILEDEEYSVEDFSSGKSLIKALTKERPSLVLLDVWVGKEDGLTILDECKKLYPSLPIVMI
SGHGTIELAVNATKKGAVDFLEKPLSIEKVIQTIESSIEKTKEVHFPNFKLDVDEILGVSPSITRVKFAVFQAAETNARV
FIYGENGTGKELTARAIHQNSKRKNEPYIEFNCASLPEESLEQELFGLELASQTDSPEIRIGKWEQAQNGTLFLDEICDL
SLALQSKVLKVILDQKLERVGGKEFIPVDVRIIAATNSNVEDAIREGKFREDLFYALNVIPLELPPLRERNQDIPLLAEY
YLKKSISENHLSQKTIDRDGLDALSSHFWPGNVRELTNIIERLSILVPGDTIKAKDVKEALHGFKRANEMVARGDLKHAK
EEFERQYIIKTLQICEGNVTRTSKALGIERTHLYRKLRSLNISVDQLIEG
>Mature_450_residues
MQKLIYILDDEKEIRKTLRVILEDEEYSVEDFSSGKSLIKALTKERPSLVLLDVWVGKEDGLTILDECKKLYPSLPIVMI
SGHGTIELAVNATKKGAVDFLEKPLSIEKVIQTIESSIEKTKEVHFPNFKLDVDEILGVSPSITRVKFAVFQAAETNARV
FIYGENGTGKELTARAIHQNSKRKNEPYIEFNCASLPEESLEQELFGLELASQTDSPEIRIGKWEQAQNGTLFLDEICDL
SLALQSKVLKVILDQKLERVGGKEFIPVDVRIIAATNSNVEDAIREGKFREDLFYALNVIPLELPPLRERNQDIPLLAEY
YLKKSISENHLSQKTIDRDGLDALSSHFWPGNVRELTNIIERLSILVPGDTIKAKDVKEALHGFKRANEMVARGDLKHAK
EEFERQYIIKTLQICEGNVTRTSKALGIERTHLYRKLRSLNISVDQLIEG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=35.2564102564103, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788550, Length=450, Percent_Identity=37.3333333333333, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790437, Length=437, Percent_Identity=35.6979405034325, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788905, Length=433, Percent_Identity=36.0277136258661, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI87082117, Length=307, Percent_Identity=42.3452768729642, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789087, Length=326, Percent_Identity=39.5705521472393, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=38.1703470031546, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=39.937106918239, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=37.4613003095975, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=36.1022364217252, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=35.4430379746835, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=34.3434343434343, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=29.8245614035088, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50969; Mature: 50969

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLIYILDDEKEIRKTLRVILEDEEYSVEDFSSGKSLIKALTKERPSLVLLDVWVGKED
CCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GLTILDECKKLYPSLPIVMISGHGTIELAVNATKKGAVDFLEKPLSIEKVIQTIESSIEK
CCHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
TKEVHFPNFKLDVDEILGVSPSITRVKFAVFQAAETNARVFIYGENGTGKELTARAIHQN
HHHCCCCCCEECHHHHHCCCCCHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHHHHHC
SKRKNEPYIEFNCASLPEESLEQELFGLELASQTDSPEIRIGKWEQAQNGTLFLDEICDL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEHHHHHHH
SLALQSKVLKVILDQKLERVGGKEFIPVDVRIIAATNSNVEDAIREGKFREDLFYALNVI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHEE
PLELPPLRERNQDIPLLAEYYLKKSISENHLSQKTIDRDGLDALSSHFWPGNVRELTNII
ECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHH
ERLSILVPGDTIKAKDVKEALHGFKRANEMVARGDLKHAKEEFERQYIIKTLQICEGNVT
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
RTSKALGIERTHLYRKLRSLNISVDQLIEG
CCHHHHCCHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MQKLIYILDDEKEIRKTLRVILEDEEYSVEDFSSGKSLIKALTKERPSLVLLDVWVGKED
CCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
GLTILDECKKLYPSLPIVMISGHGTIELAVNATKKGAVDFLEKPLSIEKVIQTIESSIEK
CCHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
TKEVHFPNFKLDVDEILGVSPSITRVKFAVFQAAETNARVFIYGENGTGKELTARAIHQN
HHHCCCCCCEECHHHHHCCCCCHHHHHHHHHEECCCCCEEEEEECCCCCHHHHHHHHHHC
SKRKNEPYIEFNCASLPEESLEQELFGLELASQTDSPEIRIGKWEQAQNGTLFLDEICDL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEHHHHHHH
SLALQSKVLKVILDQKLERVGGKEFIPVDVRIIAATNSNVEDAIREGKFREDLFYALNVI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHEE
PLELPPLRERNQDIPLLAEYYLKKSISENHLSQKTIDRDGLDALSSHFWPGNVRELTNII
ECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHH
ERLSILVPGDTIKAKDVKEALHGFKRANEMVARGDLKHAKEEFERQYIIKTLQICEGNVT
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
RTSKALGIERTHLYRKLRSLNISVDQLIEG
CCHHHHCCHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]