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The map label for this gene is leuC2 [H]

Identifier: 183221012

GI number: 183221012

Start: 1698410

End: 1699804

Strand: Direct

Name: leuC2 [H]

Synonym: LEPBI_I1625

Alternate gene names: 183221012

Gene position: 1698410-1699804 (Clockwise)

Preceding gene: 183221011

Following gene: 183221013

Centisome position: 47.18

GC content: 44.8

Gene sequence:

>1395_bases
ATGAAAACCATGTTTGAGAAGATTTGGAATGACCATTTGGTTCACGAGGATGATGGAACCTGCCTCATTTATATCGACAG
ACACCTAGTCCACGAAGTCACAAGCCCACAAGCCTTCGAAAGTTTGAAACTCACCAACCGAAAGGTGAGGCGACCCGATG
CAACATTTGCTACCATGGACCATAACGTTTCCACAAGAACGAGAGATTGGAAATCTGTGGATCCGATCTCGGTTTTGCAA
ATGCAAACCTTAATGGACAATTGTAAAGAAAACGGAATTACATTATTTGATATCAATCACCCTGACAATGGAATTGTGCA
CGTTGTCGCGCCAGAGCTTGGACTCACTCACCCTGGTATGACGATTGTTTGTGGGGATTCTCACACAGCGACACATGGTG
CCTTTGGTGCTCTCGCATTTGGAATTGGAACTTCCGAAGTGGAACATGTCTTAGCCACCCAAACCTTAGTCCAAAAGAAA
CCAAAAACTTTGGAAATCCGTGTGGATGGAACTCTCTCACCTCTGGTTTCTGCAAAAGACATCGTCCTTGCCATCATTGG
AAAAATTGGAACTGATGGTGCTACTGGTTATGTGATTGAATTCACTGGGGAAGCCATCCGTGCGCTCAGTATGGAAGGAA
GAATGACCATTTGTAATATGGCTATCGAAGCTGGTGCAAGAGCTGGACTCATTTCTCCAGACGATACGACGATCAATTAC
ATCAAAGGTAGAGATTTTGCTCCGAAAGGAGATGCATTTGATATAGCAGCTGCTAAATGGATATCTTATGCAACAGACCC
TGGTGCCAAATTTGATAAAACAGTAACACTCAATGCAAATGAAATTGCACCAATGGTTTCTTGGGGAACGTCTCCTGGAC
AAGTGATCCCAGTGACTGCAACTGTACCAAGTCCAAATGATTTCACAGATCCTGTACAAAAAAAATCTGCAGAATCTGCC
TTAGCCTATATGGACTTGAAACCTGGACAAAAACTTTCGGATGTGAAAGTAAACAAAGTATTCATCGGTTCCTGTACCAA
TTCAAGGATCGAAGACCTTCGTGTTGTGGCAAACACCGTCAAAGGGAAAAAAGTCAGTAAGGAAGTAGAAGCCATCATTG
TACCAGGTTCAGGCCGAGTGAAACGCCAAGCGGAGAGTGAAGGACTCGACAAAATTTTTATCGAAGCGGGATTCCAATGG
CGAAACCCAGGTTGTTCGATGTGCCTTGCGATGAATGATGACGTGTTATCTCCAGGGGACCGTTGTGCTTCCACTTCCAA
TCGAAACTTTGAAGGAAGGCAAGGAAAGGGTGGAAGGACCCATTTAGTGGGGCCTGCTATGGCAGCAGCAGTCGCCGTGG
AAGGACATTTTGTAGACATTCGGGAGTGGAAATAA

Upstream 100 bases:

>100_bases
ATCCACCGTCCAAACACCTTAAGAATCAATCTATTTTTCCAAATTCCCTGGACACAGACCCCCATGTCCGAGTTATGACT
TGAAGTGAGGGAGTCTAGTT

Downstream 100 bases:

>100_bases
GATGAAAGCATTTACAAAATTAAAAGGGATCGCAGCGCTTCTCGACAAAGCGAACGTAGACACAGACCAAATCATCCCAA
AACAATTCCTACGGAAAATC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKTMFEKIWNDHLVHEDDGTCLIYIDRHLVHEVTSPQAFESLKLTNRKVRRPDATFATMDHNVSTRTRDWKSVDPISVLQ
MQTLMDNCKENGITLFDINHPDNGIVHVVAPELGLTHPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKK
PKTLEIRVDGTLSPLVSAKDIVLAIIGKIGTDGATGYVIEFTGEAIRALSMEGRMTICNMAIEAGARAGLISPDDTTINY
IKGRDFAPKGDAFDIAAAKWISYATDPGAKFDKTVTLNANEIAPMVSWGTSPGQVIPVTATVPSPNDFTDPVQKKSAESA
LAYMDLKPGQKLSDVKVNKVFIGSCTNSRIEDLRVVANTVKGKKVSKEVEAIIVPGSGRVKRQAESEGLDKIFIEAGFQW
RNPGCSMCLAMNDDVLSPGDRCASTSNRNFEGRQGKGGRTHLVGPAMAAAVAVEGHFVDIREWK

Sequences:

>Translated_464_residues
MKTMFEKIWNDHLVHEDDGTCLIYIDRHLVHEVTSPQAFESLKLTNRKVRRPDATFATMDHNVSTRTRDWKSVDPISVLQ
MQTLMDNCKENGITLFDINHPDNGIVHVVAPELGLTHPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKK
PKTLEIRVDGTLSPLVSAKDIVLAIIGKIGTDGATGYVIEFTGEAIRALSMEGRMTICNMAIEAGARAGLISPDDTTINY
IKGRDFAPKGDAFDIAAAKWISYATDPGAKFDKTVTLNANEIAPMVSWGTSPGQVIPVTATVPSPNDFTDPVQKKSAESA
LAYMDLKPGQKLSDVKVNKVFIGSCTNSRIEDLRVVANTVKGKKVSKEVEAIIVPGSGRVKRQAESEGLDKIFIEAGFQW
RNPGCSMCLAMNDDVLSPGDRCASTSNRNFEGRQGKGGRTHLVGPAMAAAVAVEGHFVDIREWK
>Mature_464_residues
MKTMFEKIWNDHLVHEDDGTCLIYIDRHLVHEVTSPQAFESLKLTNRKVRRPDATFATMDHNVSTRTRDWKSVDPISVLQ
MQTLMDNCKENGITLFDINHPDNGIVHVVAPELGLTHPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKK
PKTLEIRVDGTLSPLVSAKDIVLAIIGKIGTDGATGYVIEFTGEAIRALSMEGRMTICNMAIEAGARAGLISPDDTTINY
IKGRDFAPKGDAFDIAAAKWISYATDPGAKFDKTVTLNANEIAPMVSWGTSPGQVIPVTATVPSPNDFTDPVQKKSAESA
LAYMDLKPGQKLSDVKVNKVFIGSCTNSRIEDLRVVANTVKGKKVSKEVEAIIVPGSGRVKRQAESEGLDKIFIEAGFQW
RNPGCSMCLAMNDDVLSPGDRCASTSNRNFEGRQGKGGRTHLVGPAMAAAVAVEGHFVDIREWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=27.445652173913, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI8659555, Length=397, Percent_Identity=26.7002518891688, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI41352693, Length=397, Percent_Identity=26.1964735516373, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=67.4568965517241, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI87081781, Length=343, Percent_Identity=24.198250728863, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787531, Length=405, Percent_Identity=25.4320987654321, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=370, Percent_Identity=30.5405405405405, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=370, Percent_Identity=30.5405405405405, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=26.7813267813268, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=312, Percent_Identity=30.4487179487179, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=58.8110403397028, Blast_Score=564, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6320440, Length=422, Percent_Identity=26.303317535545, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=28.3746556473829, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=395, Percent_Identity=27.0886075949367, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=26.4397905759162, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=26.4397905759162, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.4397905759162, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571643, Length=365, Percent_Identity=27.1232876712329, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=409, Percent_Identity=28.6063569682152, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137564, Length=408, Percent_Identity=26.9607843137255, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50229; Mature: 50229

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMFEKIWNDHLVHEDDGTCLIYIDRHLVHEVTSPQAFESLKLTNRKVRRPDATFATMD
CCCHHHHHCCCCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEC
HNVSTRTRDWKSVDPISVLQMQTLMDNCKENGITLFDINHPDNGIVHVVAPELGLTHPGM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCC
TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKKPKTLEIRVDGTLSPLVSAKD
EEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
IVLAIIGKIGTDGATGYVIEFTGEAIRALSMEGRMTICNMAIEAGARAGLISPDDTTINY
HHHEEEHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCEEEE
IKGRDFAPKGDAFDIAAAKWISYATDPGAKFDKTVTLNANEIAPMVSWGTSPGQVIPVTA
EECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCEEEECHHHCCCEEECCCCCCCEEEEEE
TVPSPNDFTDPVQKKSAESALAYMDLKPGQKLSDVKVNKVFIGSCTNSRIEDLRVVANTV
ECCCCCCCCCHHHHHHHHHEEEEEECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
KGKKVSKEVEAIIVPGSGRVKRQAESEGLDKIFIEAGFQWRNPGCSMCLAMNDDVLSPGD
CCCCCCCCEEEEEECCCCCCCCCHHHCCCCEEEEECCCEECCCCCCEEEEECCCCCCCCH
RCASTSNRNFEGRQGKGGRTHLVGPAMAAAVAVEGHFVDIREWK
HHCCCCCCCCCCCCCCCCCEEECCHHHHEEEEECCEEEEEECCC
>Mature Secondary Structure
MKTMFEKIWNDHLVHEDDGTCLIYIDRHLVHEVTSPQAFESLKLTNRKVRRPDATFATMD
CCCHHHHHCCCCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEC
HNVSTRTRDWKSVDPISVLQMQTLMDNCKENGITLFDINHPDNGIVHVVAPELGLTHPGM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCC
TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLVQKKPKTLEIRVDGTLSPLVSAKD
EEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
IVLAIIGKIGTDGATGYVIEFTGEAIRALSMEGRMTICNMAIEAGARAGLISPDDTTINY
HHHEEEHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEHHHHHCCCCCCCCCCCCCEEEE
IKGRDFAPKGDAFDIAAAKWISYATDPGAKFDKTVTLNANEIAPMVSWGTSPGQVIPVTA
EECCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCEEEECHHHCCCEEECCCCCCCEEEEEE
TVPSPNDFTDPVQKKSAESALAYMDLKPGQKLSDVKVNKVFIGSCTNSRIEDLRVVANTV
ECCCCCCCCCHHHHHHHHHEEEEEECCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
KGKKVSKEVEAIIVPGSGRVKRQAESEGLDKIFIEAGFQWRNPGCSMCLAMNDDVLSPGD
CCCCCCCCEEEEEECCCCCCCCCHHHCCCCEEEEECCCEECCCCCCEEEEECCCCCCCCH
RCASTSNRNFEGRQGKGGRTHLVGPAMAAAVAVEGHFVDIREWK
HHCCCCCCCCCCCCCCCCCEEECCHHHHEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA