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The map label for this gene is leuD [H]

Identifier: 183221013

GI number: 183221013

Start: 1699806

End: 1700432

Strand: Direct

Name: leuD [H]

Synonym: LEPBI_I1626

Alternate gene names: 183221013

Gene position: 1699806-1700432 (Clockwise)

Preceding gene: 183221012

Following gene: 183221014

Centisome position: 47.22

GC content: 39.39

Gene sequence:

>627_bases
ATGAAAGCATTTACAAAATTAAAAGGGATCGCAGCGCTTCTCGACAAAGCGAACGTAGACACAGACCAAATCATCCCAAA
ACAATTCCTACGGAAAATCGAAAGGTCTGGTTTTGGAAAACATTTGTTCCATGATTGGAGATTTCTCGACGATGCGGGCC
AAACACCAAACCCAGACTTTGTTCTTAACGCCAAAAGATACCAAGGAGCCAATATCCTTGTCACTCGTGATAATTTTGGG
TGTGGATCTTCCAGAGAACATGCTCCTTGGGCCTTAGAAGACTACGGCTTTCGTTCCATCCTTTCACCTTCTTATGCAGA
TATTTTTTATAATAACTGTTTTAAGAACGGGATGCTTCCAATTGTTTTACCGGAATCTCAAATCGAAGAAATTTTCCAGG
CAATTGATAAAAAACCAGGTGCCAATTTAGAAATCGATTTGGAGAACCAGGTTGTCATCACAGAAGAAGGAAAAAAATAC
CCATTTGAAGTGGATGCATTTCGAAAACACTGCCTCCTCAATGGTTTGGATGACATTGGACTCACCCTACAAAAAGCCGA
TTTTATTCAAAAATTTGAAGAAAAGAACCAAAAAGAAGTTCCCTGGTTGTACAGAAAGAGTGTATAA

Upstream 100 bases:

>100_bases
AGGCAAGGAAAGGGTGGAAGGACCCATTTAGTGGGGCCTGCTATGGCAGCAGCAGTCGCCGTGGAAGGACATTTTGTAGA
CATTCGGGAGTGGAAATAAG

Downstream 100 bases:

>100_bases
TGAAGCGCATGAAACAGTTATTTTTAACTCTTAGTTTTTTCTTCTTCGTAACGGGTCTTTCAGCAGAGGAACTGCTGATT
CAGGACACCAAACAAGGATT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKAFTKLKGIAALLDKANVDTDQIIPKQFLRKIERSGFGKHLFHDWRFLDDAGQTPNPDFVLNAKRYQGANILVTRDNFG
CGSSREHAPWALEDYGFRSILSPSYADIFYNNCFKNGMLPIVLPESQIEEIFQAIDKKPGANLEIDLENQVVITEEGKKY
PFEVDAFRKHCLLNGLDDIGLTLQKADFIQKFEEKNQKEVPWLYRKSV

Sequences:

>Translated_208_residues
MKAFTKLKGIAALLDKANVDTDQIIPKQFLRKIERSGFGKHLFHDWRFLDDAGQTPNPDFVLNAKRYQGANILVTRDNFG
CGSSREHAPWALEDYGFRSILSPSYADIFYNNCFKNGMLPIVLPESQIEEIFQAIDKKPGANLEIDLENQVVITEEGKKY
PFEVDAFRKHCLLNGLDDIGLTLQKADFIQKFEEKNQKEVPWLYRKSV
>Mature_208_residues
MKAFTKLKGIAALLDKANVDTDQIIPKQFLRKIERSGFGKHLFHDWRFLDDAGQTPNPDFVLNAKRYQGANILVTRDNFG
CGSSREHAPWALEDYGFRSILSPSYADIFYNNCFKNGMLPIVLPESQIEEIFQAIDKKPGANLEIDLENQVVITEEGKKY
PFEVDAFRKHCLLNGLDDIGLTLQKADFIQKFEEKNQKEVPWLYRKSV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=59.375, Blast_Score=252, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321429, Length=207, Percent_Identity=55.0724637681159, Blast_Score=221, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23888; Mature: 23888

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTKLKGIAALLDKANVDTDQIIPKQFLRKIERSGFGKHLFHDWRFLDDAGQTPNPDF
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNAKRYQGANILVTRDNFGCGSSREHAPWALEDYGFRSILSPSYADIFYNNCFKNGMLP
EEECCEECCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHCCCEE
IVLPESQIEEIFQAIDKKPGANLEIDLENQVVITEEGKKYPFEVDAFRKHCLLNGLDDIG
EEECHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCEEHHHHHHHHHHCCCHHHC
LTLQKADFIQKFEEKNQKEVPWLYRKSV
CCHHHHHHHHHHHHCCCCCCCCEECCCC
>Mature Secondary Structure
MKAFTKLKGIAALLDKANVDTDQIIPKQFLRKIERSGFGKHLFHDWRFLDDAGQTPNPDF
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNAKRYQGANILVTRDNFGCGSSREHAPWALEDYGFRSILSPSYADIFYNNCFKNGMLP
EEECCEECCCEEEEECCCCCCCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHCCCEE
IVLPESQIEEIFQAIDKKPGANLEIDLENQVVITEEGKKYPFEVDAFRKHCLLNGLDDIG
EEECHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCEEHHHHHHHHHHCCCHHHC
LTLQKADFIQKFEEKNQKEVPWLYRKSV
CCHHHHHHHHHHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA