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The map label for this gene is rimO

Identifier: 183220785

GI number: 183220785

Start: 1455588

End: 1456934

Strand: Direct

Name: rimO

Synonym: LEPBI_I1396

Alternate gene names: 183220785

Gene position: 1455588-1456934 (Clockwise)

Preceding gene: 183220784

Following gene: 183220786

Centisome position: 40.44

GC content: 41.13

Gene sequence:

>1347_bases
ATGCCGAAGTCTTTGGAAAACACAAACGAAACACCCAAGTCGTTTTTCATTACAACACTTGGATGCCCAAAAAACACTGT
GGATTCAATGGCCATGCACCAGTCCTTATTAAAAGAAGGACTACTTCCGGCAGCAGGCCCCGAAGCAAGTGATTTTCATT
TAGTGAATACATGTACCTTTATCCAAGATGCTACCAAAGAAACCATCCAAACCATTCTCGATTCCATTGACATTAAAAAA
CAAAATAAACAAAAGTTAGTTGTTGTTGGATGTTTTGCAGAACGTGCGGGAAAAGAAATTTCTGACGATCTACCAGAGGT
GGATCTCCATTTCGGAACAGGAAAATACGACAAAGCAGGGGAAATTTTACGAAAAAATTTCCCTCTAGAATTCAAAGACT
TAACCGAATTCAACGAAGACCTTCTCGAAAGGCTTACGACAAGTAAAGGAATTGAAAACTATTCCAAACCATATTCGTAT
GTGAAAATTTCAGATGGTTGCAATCGGGGTTGTCATTTTTGTATCATACCGAATTTACGAGGGAAATACAGAGACACAGA
TTCGAATGATGTCTTAGAACAAACAAAACTGGCGGTAAAAGCCGGTTCCAAAGAAATTTGCCTCGTCTCCCAAGACACTG
TATTTTATGGCAAAGACACTGACAAACTGATGGATTTGGTTCGTTCTGTTGCCGCTGTGGAAGGGCTTGAAATCTTACGA
CTCCTCTATTTGTATCCTGATAAAAAAACAGAAAAGTTACTCGATTTGTACAGAGAAATTCCTAAAATTGCTCCGTATTT
GGAAAGTCCCTTGCAACACGTTTCTAAATCTGTTTTAAAATCCATGAACCGCACTGGTGATTATGAATTCTTTAAATCCC
TATTCCAAAAAGCAAGGGACATCCGTCCTGATTTAGAAATCCGCACTTCGTTCATCCTTGGATTTCCTGGGGAAACCATG
GAAGATGTCGAAGAGATCATTCGATTTGTGGAAGATGTGAAACCTGAGAAAGTCAATTTGTTTCCCTACTCTCCCCAAGA
GGGAACAAAGGGTGCTACGATGGACGGCCAATTGAAGGACAAAGAGATTGCACGCCGCGTGAACCTTGTAAGGGAAGCCT
ATCTTGGAACCTTAAAGACCATCCACCAAAACCGAATTGGCAAAATTTACCCTTGTGTCGTAGATGAAGTGTTAGACGAT
GGAGCCATTGTCCGCCGCCTCCAAGATGCACCTGAAATCGATGAGGTTGTCTATGTCGAAACGAAGGATTTAAAACTGGG
CCAATTTGGAAAGGTCAGAGTGGATTCGTTTTATGAACTGGATATGTCAGGGACTTGGGTGGATTAG

Upstream 100 bases:

>100_bases
TGGTTCTTGGAAAACCAGGAAAACTTGTGAAAAAAATCTTCATCCGCAAACCAAACCCATATGATTCCACACAATCCATC
ATTGGAGAGTTAGGCGAATA

Downstream 100 bases:

>100_bases
TGGAAGATTGGAAAACCATTGCCAATATCCCAAACCTACTCACGGTTCTTAGGGTTCTTGCCTTACCGTTTTTTATCTTT
GCCTTATTCCAAAAAGAATG

Product: putative MiaB-like tRNA modifying enzyme

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPKSLENTNETPKSFFITTLGCPKNTVDSMAMHQSLLKEGLLPAAGPEASDFHLVNTCTFIQDATKETIQTILDSIDIKK
QNKQKLVVVGCFAERAGKEISDDLPEVDLHFGTGKYDKAGEILRKNFPLEFKDLTEFNEDLLERLTTSKGIENYSKPYSY
VKISDGCNRGCHFCIIPNLRGKYRDTDSNDVLEQTKLAVKAGSKEICLVSQDTVFYGKDTDKLMDLVRSVAAVEGLEILR
LLYLYPDKKTEKLLDLYREIPKIAPYLESPLQHVSKSVLKSMNRTGDYEFFKSLFQKARDIRPDLEIRTSFILGFPGETM
EDVEEIIRFVEDVKPEKVNLFPYSPQEGTKGATMDGQLKDKEIARRVNLVREAYLGTLKTIHQNRIGKIYPCVVDEVLDD
GAIVRRLQDAPEIDEVVYVETKDLKLGQFGKVRVDSFYELDMSGTWVD

Sequences:

>Translated_448_residues
MPKSLENTNETPKSFFITTLGCPKNTVDSMAMHQSLLKEGLLPAAGPEASDFHLVNTCTFIQDATKETIQTILDSIDIKK
QNKQKLVVVGCFAERAGKEISDDLPEVDLHFGTGKYDKAGEILRKNFPLEFKDLTEFNEDLLERLTTSKGIENYSKPYSY
VKISDGCNRGCHFCIIPNLRGKYRDTDSNDVLEQTKLAVKAGSKEICLVSQDTVFYGKDTDKLMDLVRSVAAVEGLEILR
LLYLYPDKKTEKLLDLYREIPKIAPYLESPLQHVSKSVLKSMNRTGDYEFFKSLFQKARDIRPDLEIRTSFILGFPGETM
EDVEEIIRFVEDVKPEKVNLFPYSPQEGTKGATMDGQLKDKEIARRVNLVREAYLGTLKTIHQNRIGKIYPCVVDEVLDD
GAIVRRLQDAPEIDEVVYVETKDLKLGQFGKVRVDSFYELDMSGTWVD
>Mature_447_residues
PKSLENTNETPKSFFITTLGCPKNTVDSMAMHQSLLKEGLLPAAGPEASDFHLVNTCTFIQDATKETIQTILDSIDIKKQ
NKQKLVVVGCFAERAGKEISDDLPEVDLHFGTGKYDKAGEILRKNFPLEFKDLTEFNEDLLERLTTSKGIENYSKPYSYV
KISDGCNRGCHFCIIPNLRGKYRDTDSNDVLEQTKLAVKAGSKEICLVSQDTVFYGKDTDKLMDLVRSVAAVEGLEILRL
LYLYPDKKTEKLLDLYREIPKIAPYLESPLQHVSKSVLKSMNRTGDYEFFKSLFQKARDIRPDLEIRTSFILGFPGETME
DVEEIIRFVEDVKPEKVNLFPYSPQEGTKGATMDGQLKDKEIARRVNLVREAYLGTLKTIHQNRIGKIYPCVVDEVLDDG
AIVRRLQDAPEIDEVVYVETKDLKLGQFGKVRVDSFYELDMSGTWVD

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=350, Percent_Identity=25.7142857142857, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI28872784, Length=376, Percent_Identity=23.6702127659574, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI28872782, Length=376, Percent_Identity=23.6702127659574, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=32.3076923076923, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=26.2331838565022, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71996771, Length=293, Percent_Identity=27.6450511945392, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356207, Length=394, Percent_Identity=24.6192893401015, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=25.2771618625277, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_LEPBA (B0SGD8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962440.1
- ProteinModelPortal:   B0SGD8
- SMR:   B0SGD8
- GeneID:   6387875
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1342
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK573783
- BioCyc:   LBIF355278:LBF_1342-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50833; Mature: 50702

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSLENTNETPKSFFITTLGCPKNTVDSMAMHQSLLKEGLLPAAGPEASDFHLVNTCTF
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHH
IQDATKETIQTILDSIDIKKQNKQKLVVVGCFAERAGKEISDDLPEVDLHFGTGKYDKAG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCCHHHCCCCCCEEECCCCCCCHHH
EILRKNFPLEFKDLTEFNEDLLERLTTSKGIENYSKPYSYVKISDGCNRGCHFCIIPNLR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCEEEEECCCC
GKYRDTDSNDVLEQTKLAVKAGSKEICLVSQDTVFYGKDTDKLMDLVRSVAAVEGLEILR
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLYLYPDKKTEKLLDLYREIPKIAPYLESPLQHVSKSVLKSMNRTGDYEFFKSLFQKARD
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
IRPDLEIRTSFILGFPGETMEDVEEIIRFVEDVKPEKVNLFPYSPQEGTKGATMDGQLKD
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCH
KEIARRVNLVREAYLGTLKTIHQNRIGKIYPCVVDEVLDDGAIVRRLQDAPEIDEVVYVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEE
TKDLKLGQFGKVRVDSFYELDMSGTWVD
ECCCCCCCCCCEEECCEEEECCCCCCCC
>Mature Secondary Structure 
PKSLENTNETPKSFFITTLGCPKNTVDSMAMHQSLLKEGLLPAAGPEASDFHLVNTCTF
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHH
IQDATKETIQTILDSIDIKKQNKQKLVVVGCFAERAGKEISDDLPEVDLHFGTGKYDKAG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHCCCHHHCCCCCCEEECCCCCCCHHH
EILRKNFPLEFKDLTEFNEDLLERLTTSKGIENYSKPYSYVKISDGCNRGCHFCIIPNLR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCEEEEECCCC
GKYRDTDSNDVLEQTKLAVKAGSKEICLVSQDTVFYGKDTDKLMDLVRSVAAVEGLEILR
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLYLYPDKKTEKLLDLYREIPKIAPYLESPLQHVSKSVLKSMNRTGDYEFFKSLFQKARD
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
IRPDLEIRTSFILGFPGETMEDVEEIIRFVEDVKPEKVNLFPYSPQEGTKGATMDGQLKD
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCH
KEIARRVNLVREAYLGTLKTIHQNRIGKIYPCVVDEVLDDGAIVRRLQDAPEIDEVVYVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEE
TKDLKLGQFGKVRVDSFYELDMSGTWVD
ECCCCCCCCCCEEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA