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The map label for this gene is leuD [H]

Identifier: 183220624

GI number: 183220624

Start: 1281131

End: 1281757

Strand: Direct

Name: leuD [H]

Synonym: LEPBI_I1230

Alternate gene names: 183220624

Gene position: 1281131-1281757 (Clockwise)

Preceding gene: 183220623

Following gene: 183220629

Centisome position: 35.59

GC content: 40.35

Gene sequence:

>627_bases
ATGAGTGTAAAAAGTTGGACAGTCCATACGGGAGTGGCGGTATCCATGCCCAGAGAAGATATTGATACGGACCAAATACT
TCCAAAACAATTTATGAAATTAATTGATAAGAAAGGTTTTGGAAACCATTTGTTTCATGATTGGCGGTATTTAGACTGGG
CGGGAAAGATTCCTAATCCCGAATTCATTTTGAACCAAGAAACATTTCAGAATGCGAGTGTGCTCATTGCTGGAAAAAAT
TTCGGATGCGGCTCGAGTCGAGAACATGCACCTTGGGCCCTTTCTGATTTCGGATTCCGAGTGATCATCGCTCCTTCTTT
TGCCGACATCTTTTCCATCAATTCTGCGAAGAATGGGATTGCTCTTGTTCGATTGAAAGATGAAGAGGTTTCTTCTCTTC
ATCACTGGGTTAGGAATCATTCTGGATCCCCAATTAGGATCAATTTGGAATCATTTGAAGTAAAAGCAGGTGAGCAAACC
TACTCTTTTTATTTGGATTCTGGTTCCACCAATCGGATCCGAAATGGTTGGGATGATATCGATACCACGCTAAACAATGA
GAGAGAGATATTGGAATTCGAAAGGAAACAAAAAATGGAAACTCCATTTTTAGAAGTGCATTGGTAA

Upstream 100 bases:

>100_bases
AAGGAAGACAAGGTCGAGGAGGGAGGACACATTTAGTCAGTCCTTCCATGGCAGCTGCCGCAGCAGTGACTGGTAAATTG
ACAGATGTGAGGGAATTAGC

Downstream 100 bases:

>100_bases
TTTGTTTGAAAGTCCAAATATTAGATCCTATGAACCTTTCTCTTACCTTGCGGAAAGTTTGCTAGGATTTTCTATAGGAA
TACTCCTTTTTACAGAGAGG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSVKSWTVHTGVAVSMPREDIDTDQILPKQFMKLIDKKGFGNHLFHDWRYLDWAGKIPNPEFILNQETFQNASVLIAGKN
FGCGSSREHAPWALSDFGFRVIIAPSFADIFSINSAKNGIALVRLKDEEVSSLHHWVRNHSGSPIRINLESFEVKAGEQT
YSFYLDSGSTNRIRNGWDDIDTTLNNEREILEFERKQKMETPFLEVHW

Sequences:

>Translated_208_residues
MSVKSWTVHTGVAVSMPREDIDTDQILPKQFMKLIDKKGFGNHLFHDWRYLDWAGKIPNPEFILNQETFQNASVLIAGKN
FGCGSSREHAPWALSDFGFRVIIAPSFADIFSINSAKNGIALVRLKDEEVSSLHHWVRNHSGSPIRINLESFEVKAGEQT
YSFYLDSGSTNRIRNGWDDIDTTLNNEREILEFERKQKMETPFLEVHW
>Mature_207_residues
SVKSWTVHTGVAVSMPREDIDTDQILPKQFMKLIDKKGFGNHLFHDWRYLDWAGKIPNPEFILNQETFQNASVLIAGKNF
GCGSSREHAPWALSDFGFRVIIAPSFADIFSINSAKNGIALVRLKDEEVSSLHHWVRNHSGSPIRINLESFEVKAGEQTY
SFYLDSGSTNRIRNGWDDIDTTLNNEREILEFERKQKMETPFLEVHW

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=189, Percent_Identity=50.7936507936508, Blast_Score=205, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=208, Percent_Identity=37.0192307692308, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23914; Mature: 23783

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKSWTVHTGVAVSMPREDIDTDQILPKQFMKLIDKKGFGNHLFHDWRYLDWAGKIPNP
CCCCCEEEECCEEEECCCCCCCCHHCCHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCC
EFILNQETFQNASVLIAGKNFGCGSSREHAPWALSDFGFRVIIAPSFADIFSINSAKNGI
CEEECHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCCHHHEECCCCCCCE
ALVRLKDEEVSSLHHWVRNHSGSPIRINLESFEVKAGEQTYSFYLDSGSTNRIRNGWDDI
EEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCHH
DTTLNNEREILEFERKQKMETPFLEVHW
HHHCCCCHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SVKSWTVHTGVAVSMPREDIDTDQILPKQFMKLIDKKGFGNHLFHDWRYLDWAGKIPNP
CCCCEEEECCEEEECCCCCCCCHHCCHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCC
EFILNQETFQNASVLIAGKNFGCGSSREHAPWALSDFGFRVIIAPSFADIFSINSAKNGI
CEEECHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCCHHHEECCCCCCCE
ALVRLKDEEVSSLHHWVRNHSGSPIRINLESFEVKAGEQTYSFYLDSGSTNRIRNGWDDI
EEEEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCHH
DTTLNNEREILEFERKQKMETPFLEVHW
HHHCCCCHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA