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The map label for this gene is leuC1 [H]

Identifier: 183220623

GI number: 183220623

Start: 1279731

End: 1281134

Strand: Direct

Name: leuC1 [H]

Synonym: LEPBI_I1229

Alternate gene names: 183220623

Gene position: 1279731-1281134 (Clockwise)

Preceding gene: 183220621

Following gene: 183220624

Centisome position: 35.55

GC content: 42.02

Gene sequence:

>1404_bases
ATGGGACAAACTCTATATGATAAAATTTGGGAACGTCATCGGATCTTGGAGAATTCAGACTCTGAATCGATTTTATATGT
GGATCGACATATCCTGCATGAAGTGACTTCTGCCCAAGCATTCGAAGGATTAAGAACGAAAAAAAGAGATGTTCGAAGGA
AGGATCTCACCTTCGGAGTTGTGGATCATAATGTTTCTACAAAAGATCGTAAAAACAGAGATGCTGCAGGACCAGTCTCC
CGACTACAAATTGAGACAATGGAGAAAAACTGTTTTGATTTTGGAATTCGTTTGTTCGGTCCAGACGATCCAAATCAAGG
GATTGTACATGTGGTGGGTCCTGAGTTAGGATTTACAACTCCAGGATCTGTGATCGTTTGTGGAGATTCTCATACAGCAA
CTCATGGAGCCTTTGGTGCATTGGCCTTTGGAATAGGGACAAGTGAAGTCGAACATGTGTTAGCGACTCAAAGTCTCAAA
CAGTCGAAAACAAAATCTATGCTCGTCCAATTCCATGGGAAACCTGGATTTGGAATCACATCTAAAGATATCATACTTGC
GCTCATTAAAAAGATGGGAACATCCGGAGGAAGGGGTTTCACAATCGAATATGGAGGTGAATGGGTACGTTCACTATCTA
TGGAAGGCAGAATGACTCTTTGTAATATGAGTATCGAAGCGGGAGCAAGGGCAAGCCTCATCGCTCCGGACCAAATGACA
TTTAATTATCTGAAAGATAAAAAACTAGTTCCCCAAGGTGAGAGATTTAAAGAAGCAATCGAATATTGGAAAACTTTTTT
CACAGATAAAGATGCCGTTTTTGATGAGATAATTGAATTCGATATTTCTAATATAAAACCGCAGGTCACATGGGGAACCA
ATCCATCACAGACGATATCCATTGATGAAATGATCCCGAATCCTGAAGATTTCATTGAGGGACGAGCAAGAGAAACAGCA
GCCAATGCCTTGGCGTATATGGATTTAAAACCAGGAACTCCAATTTCGGAGATTCAGATCGATAAGGTGTTCATTGGCTC
TTGTACAAATGCAAGGATAGAAGATTTACGTTTTGCGGCAGAAGTAACAAAAGGAAAAAAAGTCCATCCAAGAGTGCAAG
CCTTGGTCGTTCCAGGTTCAGGATCAGTGAAAGCACAGGCTGAATCAGAAGGTTTGGATACAATTTTCAAAGATGCTGGA
TTCGAATGGAGAGAACCTGGTTGTTCCCTTTGTCTTGCAATGAACGACGATGTATTACTACCAGGAGAAAGGTGTGCCTC
CACTTCTAACCGCAACTTTGAAGGAAGACAAGGTCGAGGAGGGAGGACACATTTAGTCAGTCCTTCCATGGCAGCTGCCG
CAGCAGTGACTGGTAAATTGACAGATGTGAGGGAATTAGCATGA

Upstream 100 bases:

>100_bases
GAATTCCATACTACATATAACAATTAGTTATATATATTATAAAACCAATTTATTTGCATTATATCAATTATTTTGATACT
CTTATTACCAAGAGGAAAAT

Downstream 100 bases:

>100_bases
GTGTAAAAAGTTGGACAGTCCATACGGGAGTGGCGGTATCCATGCCCAGAGAAGATATTGATACGGACCAAATACTTCCA
AAACAATTTATGAAATTAAT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MGQTLYDKIWERHRILENSDSESILYVDRHILHEVTSAQAFEGLRTKKRDVRRKDLTFGVVDHNVSTKDRKNRDAAGPVS
RLQIETMEKNCFDFGIRLFGPDDPNQGIVHVVGPELGFTTPGSVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQSLK
QSKTKSMLVQFHGKPGFGITSKDIILALIKKMGTSGGRGFTIEYGGEWVRSLSMEGRMTLCNMSIEAGARASLIAPDQMT
FNYLKDKKLVPQGERFKEAIEYWKTFFTDKDAVFDEIIEFDISNIKPQVTWGTNPSQTISIDEMIPNPEDFIEGRARETA
ANALAYMDLKPGTPISEIQIDKVFIGSCTNARIEDLRFAAEVTKGKKVHPRVQALVVPGSGSVKAQAESEGLDTIFKDAG
FEWREPGCSLCLAMNDDVLLPGERCASTSNRNFEGRQGRGGRTHLVSPSMAAAAAVTGKLTDVRELA

Sequences:

>Translated_467_residues
MGQTLYDKIWERHRILENSDSESILYVDRHILHEVTSAQAFEGLRTKKRDVRRKDLTFGVVDHNVSTKDRKNRDAAGPVS
RLQIETMEKNCFDFGIRLFGPDDPNQGIVHVVGPELGFTTPGSVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQSLK
QSKTKSMLVQFHGKPGFGITSKDIILALIKKMGTSGGRGFTIEYGGEWVRSLSMEGRMTLCNMSIEAGARASLIAPDQMT
FNYLKDKKLVPQGERFKEAIEYWKTFFTDKDAVFDEIIEFDISNIKPQVTWGTNPSQTISIDEMIPNPEDFIEGRARETA
ANALAYMDLKPGTPISEIQIDKVFIGSCTNARIEDLRFAAEVTKGKKVHPRVQALVVPGSGSVKAQAESEGLDTIFKDAG
FEWREPGCSLCLAMNDDVLLPGERCASTSNRNFEGRQGRGGRTHLVSPSMAAAAAVTGKLTDVRELA
>Mature_466_residues
GQTLYDKIWERHRILENSDSESILYVDRHILHEVTSAQAFEGLRTKKRDVRRKDLTFGVVDHNVSTKDRKNRDAAGPVSR
LQIETMEKNCFDFGIRLFGPDDPNQGIVHVVGPELGFTTPGSVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQSLKQ
SKTKSMLVQFHGKPGFGITSKDIILALIKKMGTSGGRGFTIEYGGEWVRSLSMEGRMTLCNMSIEAGARASLIAPDQMTF
NYLKDKKLVPQGERFKEAIEYWKTFFTDKDAVFDEIIEFDISNIKPQVTWGTNPSQTISIDEMIPNPEDFIEGRARETAA
NALAYMDLKPGTPISEIQIDKVFIGSCTNARIEDLRFAAEVTKGKKVHPRVQALVVPGSGSVKAQAESEGLDTIFKDAGF
EWREPGCSLCLAMNDDVLLPGERCASTSNRNFEGRQGRGGRTHLVSPSMAAAAAVTGKLTDVRELA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=449, Percent_Identity=24.2761692650334, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=28.3378746594005, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI41352693, Length=399, Percent_Identity=25.31328320802, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=62.0171673819742, Blast_Score=606, Evalue=1e-175,
Organism=Escherichia coli, GI1787531, Length=447, Percent_Identity=24.8322147651007, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081781, Length=380, Percent_Identity=25.5263157894737, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI2367097, Length=396, Percent_Identity=27.5252525252525, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=29.5890410958904, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=29.5890410958904, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=29.6052631578947, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=454, Percent_Identity=25.3303964757709, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=54.6808510638298, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322261, Length=409, Percent_Identity=27.8728606356968, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=366, Percent_Identity=29.2349726775956, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=465, Percent_Identity=25.1612903225806, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281365315, Length=385, Percent_Identity=28.051948051948, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17864292, Length=385, Percent_Identity=28.051948051948, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161076999, Length=385, Percent_Identity=28.051948051948, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571643, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.7543859649123, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17137564, Length=520, Percent_Identity=26.3461538461538, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51336; Mature: 51205

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTLYDKIWERHRILENSDSESILYVDRHILHEVTSAQAFEGLRTKKRDVRRKDLTFGV
CCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
VDHNVSTKDRKNRDAAGPVSRLQIETMEKNCFDFGIRLFGPDDPNQGIVHVVGPELGFTT
EECCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCEEEECCCCCCCCEEEEECCCCCCCC
PGSVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQSLKQSKTKSMLVQFHGKPGFGIT
CCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
SKDIILALIKKMGTSGGRGFTIEYGGEWVRSLSMEGRMTLCNMSIEAGARASLIAPDQMT
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHH
FNYLKDKKLVPQGERFKEAIEYWKTFFTDKDAVFDEIIEFDISNIKPQVTWGTNPSQTIS
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
IDEMIPNPEDFIEGRARETAANALAYMDLKPGTPISEIQIDKVFIGSCTNARIEDLRFAA
HHHHCCCHHHHHCCCHHHHHHHEEEEEECCCCCCHHHEEEEEEEEECCCCCHHHHHHHHH
EVTKGKKVHPRVQALVVPGSGSVKAQAESEGLDTIFKDAGFEWREPGCSLCLAMNDDVLL
HHHCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEC
PGERCASTSNRNFEGRQGRGGRTHLVSPSMAAAAAVTGKLTDVRELA
CCHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
GQTLYDKIWERHRILENSDSESILYVDRHILHEVTSAQAFEGLRTKKRDVRRKDLTFGV
CCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
VDHNVSTKDRKNRDAAGPVSRLQIETMEKNCFDFGIRLFGPDDPNQGIVHVVGPELGFTT
EECCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCEEEECCCCCCCCEEEEECCCCCCCC
PGSVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQSLKQSKTKSMLVQFHGKPGFGIT
CCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
SKDIILALIKKMGTSGGRGFTIEYGGEWVRSLSMEGRMTLCNMSIEAGARASLIAPDQMT
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHH
FNYLKDKKLVPQGERFKEAIEYWKTFFTDKDAVFDEIIEFDISNIKPQVTWGTNPSQTIS
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
IDEMIPNPEDFIEGRARETAANALAYMDLKPGTPISEIQIDKVFIGSCTNARIEDLRFAA
HHHHCCCHHHHHCCCHHHHHHHEEEEEECCCCCCHHHEEEEEEEEECCCCCHHHHHHHHH
EVTKGKKVHPRVQALVVPGSGSVKAQAESEGLDTIFKDAGFEWREPGCSLCLAMNDDVLL
HHHCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEC
PGERCASTSNRNFEGRQGRGGRTHLVSPSMAAAAAVTGKLTDVRELA
CCHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA