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The map label for this gene is sasA [H]

Identifier: 183220595

GI number: 183220595

Start: 1249924

End: 1251606

Strand: Direct

Name: sasA [H]

Synonym: LEPBI_I1199

Alternate gene names: 183220595

Gene position: 1249924-1251606 (Clockwise)

Preceding gene: 183220594

Following gene: 183220597

Centisome position: 34.72

GC content: 35.53

Gene sequence:

>1683_bases
ATGGACTGGAAGTCAATTGTAAAAGGTATCTTCCTATTTTGTGCATATATTGGTTCTGCCAAATTGGGTATGGAATATTT
TGCCTTCCATCCTATGAATTTGGCCGTACTTTGGATTCCTTCTGGAATCGGTCTCATTGGATGTTTGTTTTTTGGATACA
GGTTCCTTCCTTTTGTTTGGATTGCCAGTTTTATCGCAAACAAAGATGGACTCATCAGTAGCCACAATGGTCTTTCTCCC
TTTTTACTTTATCTAAGCATTGTACTAACTGCCTCAGTGGATGCATTTCAATCTGGGTTATCGTATTTTTTTTGGACTCA
AAAAATTCGTAAAAACCTCAATTCAACAAAAGACAATTTTTACTTCATCGTCTATGTTTCTTTTTTATCCAGTTTGATCT
CAATTATGATCCTTGGGGTCGTTCTCAATTCATTTGGATACTTCCAAAGTTTAAGTTCAAATGATATTTTTAGAACTCTC
CTTGTTATCACGTTTGGCGACACTATTGGAATCTTTATCACGGTTCCATTGTTTATGGCATGGAGAAAGTTGCTTTGGAA
AGATCTTTCGTTTCGACTTATTTTTTGGACCATGGTTTTTGTCATTTTCCAAGCATTGGTTGTCTATCTATTCCCTTACC
TTTTTTTCTTGTCCTTTCTTGTTCTCATATATTTGGGTTATCGTTTTCAAATCCGTGGTGTGACTCTCGGTGTATTTTTT
TTATATCTATCCAGTATCATGATGACACGGTTGGGAATAGGTCCCTTTGTCCAACCAAATATTTTTGATTCATATATCTA
TTTAATATCGTTTTTAAATCCTTATGCCATCCTTGCGGAATTCATCACCTTACAATACCAAAGGTTACTTTCGAATCGAT
TTGAATTGGAAAAAAAAGTTTATGACCGAACCAAATTACTGAGAACTCAAATTTTTGAAAAAAACCAAGCAATCGAAGCA
TTACATAATTCAGAAAATTTATTAAGTGAATCTAATCGCACAAAAGATGTTTTTTTTTCCATCATTGCTCATGATTTAAG
AAATCCACTTGGTTCGTTTAAACAAATCACTGAGCTATTGTATACAGAATTTGATACGTATTCAGATTCCGAAAAAAAGG
AAACAATTTTTGAAATTCAAAAATCCGCATCAAGAGTGTACGGATTATTAGAACAACTTTTGGATTGGGCAAGGACTCAA
ACTGGAAATATGCCATTTCGTCCCACCGAGATCAATTTACGAACGGTTGTTTCCAAAATCACCGACCAAATGCTTGCGAC
AATTCAGAAAAAAGGAATTCAATTCTTGGTAGAGATTCCAGAAAAGTTTTCATTTGTTTATGCCGATTCTGAAATGATCC
AAGCAGTCCTTAGGAACTTGATTTCCAACTCTATTAAATTCACGAATGAAAATGGTTATATCCAAATATCCGCCTCACAT
GAAGAAGATGGAGTCCGAATCGAATGTAAAGATAACGGTGTGGGGATGGACAGTTCCGACTTAGAAAAACTGTTTCGTTT
GGATGCACAATTGTCTAGGATCGGTCTAGAAGGGGAAAAAGGAACTGGGCTTGGACTCATCCTTTGTCATGAATTCATCA
AATTACATGGTGGTGAAATCTGGGCTAAAAGTGAAAAAGGGAAAGGAACAACTGTTAGTTTTCGATTACCTGATCCCGTT
TAA

Upstream 100 bases:

>100_bases
ATCATAATTGATTGTTAAATTTCAGAAAACTTTAAATTTAAAATAATTAAATGTTGTCTTTTTTTTAATCTACGATTCCC
TATTTTTTTAAGATTCACTT

Downstream 100 bases:

>100_bases
TACATCCCTAAAAATGGAAATGTCAAACTGATTCATTTTTGATAACGTTTGTTTTGTCAAGGTAAGGCAAATAAACAATG
AATTTAGTATTCCCTTTCGT

Product: putative two-component sensor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MDWKSIVKGIFLFCAYIGSAKLGMEYFAFHPMNLAVLWIPSGIGLIGCLFFGYRFLPFVWIASFIANKDGLISSHNGLSP
FLLYLSIVLTASVDAFQSGLSYFFWTQKIRKNLNSTKDNFYFIVYVSFLSSLISIMILGVVLNSFGYFQSLSSNDIFRTL
LVITFGDTIGIFITVPLFMAWRKLLWKDLSFRLIFWTMVFVIFQALVVYLFPYLFFLSFLVLIYLGYRFQIRGVTLGVFF
LYLSSIMMTRLGIGPFVQPNIFDSYIYLISFLNPYAILAEFITLQYQRLLSNRFELEKKVYDRTKLLRTQIFEKNQAIEA
LHNSENLLSESNRTKDVFFSIIAHDLRNPLGSFKQITELLYTEFDTYSDSEKKETIFEIQKSASRVYGLLEQLLDWARTQ
TGNMPFRPTEINLRTVVSKITDQMLATIQKKGIQFLVEIPEKFSFVYADSEMIQAVLRNLISNSIKFTNENGYIQISASH
EEDGVRIECKDNGVGMDSSDLEKLFRLDAQLSRIGLEGEKGTGLGLILCHEFIKLHGGEIWAKSEKGKGTTVSFRLPDPV

Sequences:

>Translated_560_residues
MDWKSIVKGIFLFCAYIGSAKLGMEYFAFHPMNLAVLWIPSGIGLIGCLFFGYRFLPFVWIASFIANKDGLISSHNGLSP
FLLYLSIVLTASVDAFQSGLSYFFWTQKIRKNLNSTKDNFYFIVYVSFLSSLISIMILGVVLNSFGYFQSLSSNDIFRTL
LVITFGDTIGIFITVPLFMAWRKLLWKDLSFRLIFWTMVFVIFQALVVYLFPYLFFLSFLVLIYLGYRFQIRGVTLGVFF
LYLSSIMMTRLGIGPFVQPNIFDSYIYLISFLNPYAILAEFITLQYQRLLSNRFELEKKVYDRTKLLRTQIFEKNQAIEA
LHNSENLLSESNRTKDVFFSIIAHDLRNPLGSFKQITELLYTEFDTYSDSEKKETIFEIQKSASRVYGLLEQLLDWARTQ
TGNMPFRPTEINLRTVVSKITDQMLATIQKKGIQFLVEIPEKFSFVYADSEMIQAVLRNLISNSIKFTNENGYIQISASH
EEDGVRIECKDNGVGMDSSDLEKLFRLDAQLSRIGLEGEKGTGLGLILCHEFIKLHGGEIWAKSEKGKGTTVSFRLPDPV
>Mature_560_residues
MDWKSIVKGIFLFCAYIGSAKLGMEYFAFHPMNLAVLWIPSGIGLIGCLFFGYRFLPFVWIASFIANKDGLISSHNGLSP
FLLYLSIVLTASVDAFQSGLSYFFWTQKIRKNLNSTKDNFYFIVYVSFLSSLISIMILGVVLNSFGYFQSLSSNDIFRTL
LVITFGDTIGIFITVPLFMAWRKLLWKDLSFRLIFWTMVFVIFQALVVYLFPYLFFLSFLVLIYLGYRFQIRGVTLGVFF
LYLSSIMMTRLGIGPFVQPNIFDSYIYLISFLNPYAILAEFITLQYQRLLSNRFELEKKVYDRTKLLRTQIFEKNQAIEA
LHNSENLLSESNRTKDVFFSIIAHDLRNPLGSFKQITELLYTEFDTYSDSEKKETIFEIQKSASRVYGLLEQLLDWARTQ
TGNMPFRPTEINLRTVVSKITDQMLATIQKKGIQFLVEIPEKFSFVYADSEMIQAVLRNLISNSIKFTNENGYIQISASH
EEDGVRIECKDNGVGMDSSDLEKLFRLDAQLSRIGLEGEKGTGLGLILCHEFIKLHGGEIWAKSEKGKGTTVSFRLPDPV

Specific function: May be involved in signal transduction. Participates in the kaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria, via its interaction with kaiC. Required for robustness of the circadian rhythm of gene expression an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=28.3464566929134, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=27.6859504132231, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=27.0386266094421, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI48994928, Length=241, Percent_Identity=26.5560165975104, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081816, Length=275, Percent_Identity=26.1818181818182, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=25.9414225941423, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=23.6286919831224, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=26.1224489795918, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=28.0542986425339, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=22.8448275862069, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=26.4069264069264, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=25.5707762557078, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=297, Percent_Identity=25.5892255892256, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011649
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF07689 KaiB [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64307; Mature: 64307

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCCCEEEEECCCCC
>Mature Secondary Structure
MDWKSIVKGIFLFCAYIGSAKLGMEYFAFHPMNLAVLWIPSGIGLIGCLFFGYRFLPFVW
CCHHHHHHHHHHHHHHHCCHHHCCHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
IASFIANKDGLISSHNGLSPFLLYLSIVLTASVDAFQSGLSYFFWTQKIRKNLNSTKDNF
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
YFIVYVSFLSSLISIMILGVVLNSFGYFQSLSSNDIFRTLLVITFGDTIGIFITVPLFMA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
WRKLLWKDLSFRLIFWTMVFVIFQALVVYLFPYLFFLSFLVLIYLGYRFQIRGVTLGVFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
LYLSSIMMTRLGIGPFVQPNIFDSYIYLISFLNPYAILAEFITLQYQRLLSNRFELEKKV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDRTKLLRTQIFEKNQAIEALHNSENLLSESNRTKDVFFSIIAHDLRNPLGSFKQITELL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTEFDTYSDSEKKETIFEIQKSASRVYGLLEQLLDWARTQTGNMPFRPTEINLRTVVSKI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
TDQMLATIQKKGIQFLVEIPEKFSFVYADSEMIQAVLRNLISNSIKFTNENGYIQISASH
HHHHHHHHHHHHHHHHHHCHHHHEEEEECHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
EEDGVRIECKDNGVGMDSSDLEKLFRLDAQLSRIGLEGEKGTGLGLILCHEFIKLHGGEI
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCE
WAKSEKGKGTTVSFRLPDPV
EEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]