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The map label for this gene is cysI

Identifier: 183220577

GI number: 183220577

Start: 1228461

End: 1230128

Strand: Direct

Name: cysI

Synonym: LEPBI_I1181

Alternate gene names: 183220577

Gene position: 1228461-1230128 (Clockwise)

Preceding gene: 183220576

Following gene: 183220585

Centisome position: 34.13

GC content: 39.87

Gene sequence:

>1668_bases
ATGGCAGAAACTAAAAAAGAAACTCTCGCAGAAAAAGTAAAAAGACTAAGCCGGGGCCTTCGAGGATCACTCGTCGATAG
TTTAAAGGATGAACATACTGGTTCTTTACGTTCTGATGACCAACTTTTACTTAAGTTTCATGGAATGTACCAACAAGATG
ATCGGGATCGAAGAGATGAACGTGCTCTTAAAAAATTAGAACGTCTATATTCCTTTATGATTCGCTTGAGGATTCCTGGT
GGAATGATTGGCCCTGTACACTGGGAAGCCTTACATAATGTAGCTGGTGAAAATTCCACTGGAACGATCAAAATCACCAC
AAGACAAACCGTCCAACTCCATGGTATTTTAAAATCCAAAATCAAACCAACCATCAAAGCATTTGATTCTGTATTTTTGG
ATTCCATCGCGGCTTGTGGAGATGTCAATCGTAATGTTACATGTACTTCGAATCCAGCGGCAAGCCCCCTCCACAAAGAG
GTGTTTGGTTATGCAGGGGAAATCAGTCGTTCCTTACTTCCCAAAACGAGAGCGTATTACGAAATTTGGTTAGATGAAAA
TCTTTTAGCAGAAAAAGAAGAACCAGAAGATCCTTTGTATAAGGATGTTTACCTTCCTCGTAAATTCAAAATCGCCATCG
CGATCCCGCCGTATAACGATGTGGATCTTTTTACAAACGACATTGGTCTCATCGCCATCATAGAAAATGGACAATTATTG
GGATTCAATGTAGCAGTGGGGGGAGGACTTGGAACAACTCATGGGAATCCCGATACTTATCCTAGGGTTGGAACTGTATT
TGGTTTTATTCCTAAAAAAGACATATTACGTGTTGTTTACGAGATTGTCACTGTCCAAAGAGATTTTGGAAACAGAGAAG
ACCGCAAATTGTCTCGATTGAAATACACATTGGACCGGTTAGGTGTTGAGTTTTACAAACGTGAAGTTGAAAAACGTGCA
GGTATTAGTTTTGAGTCAGCAAAAGATTTTCAATTCACAACAAGGTCTGATGATTTTGGATGGAAACAAGATGCGGCAGG
AAACTGGCATTATACCGTATTTGTTGAAAATGGAAGGGTCTGTGACGAACATGGGTATAATCTAAAAACAGCTCTTCTTG
AAGTTTCCAAAACAAGACGTGCAACATTTCGTTTCACTTGTAACCAAAACTTGATTTTGTCCGATATCTTTCCAAAGGAC
AAGGATCTCATTGAATCGATTCTTGTAAAATTTGGAGTCCATCGAAAGACTGCTGAGGTATCTCCTATCCGTAAAAATTC
CATTGCTTGTGTTGCGTTAAACACTTGTTCATTGGCGCTTGCGGAAGCACAAAGATACCTGCCAAGCCTCATTGATAAAA
TCGAACCAATCCTTTCCAAACATGGGCTATCAGAGGAACCAATTTCCATTCGGATGACAGGTTGCCCCAATGGATGTGCA
AGGCCTTATATCTCCGAAATTGGACTTGTGGGAACATCCTACGGTAAATACAATTTACACGTAGGTGCCGACGCAGAAGG
ATACCGTCTCAATAAAAAGTACAAAGAAGATTTGGATGAGTCGGCAATCTTACAAGAATTAGATGGTCTATTTGGAAAAT
TTTCAAAGGATCGAAAAGGTAAGGAATCCTTTGGAGATTATATCAATCGGATTGGGATTTTGAAATAA

Upstream 100 bases:

>100_bases
TTATCGGAAAGAACTTTTGATACTGGAAAAGAAGCTTCTGATTATCTAAAAGAATTGGAAGAAAACGGCCGTTACATCAA
GGACGTTTACTGAGGAACCC

Downstream 100 bases:

>100_bases
GAATCGAAACCAAACGAAACCAATGGTTCAAGGATATTGGATCATTGGATGTATTTTTTTCATAAAATTTCCCACCATAA
AACAGCGTATACCTATCCAA

Product: sulfite reductase hemoprotein subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MAETKKETLAEKVKRLSRGLRGSLVDSLKDEHTGSLRSDDQLLLKFHGMYQQDDRDRRDERALKKLERLYSFMIRLRIPG
GMIGPVHWEALHNVAGENSTGTIKITTRQTVQLHGILKSKIKPTIKAFDSVFLDSIAACGDVNRNVTCTSNPAASPLHKE
VFGYAGEISRSLLPKTRAYYEIWLDENLLAEKEEPEDPLYKDVYLPRKFKIAIAIPPYNDVDLFTNDIGLIAIIENGQLL
GFNVAVGGGLGTTHGNPDTYPRVGTVFGFIPKKDILRVVYEIVTVQRDFGNREDRKLSRLKYTLDRLGVEFYKREVEKRA
GISFESAKDFQFTTRSDDFGWKQDAAGNWHYTVFVENGRVCDEHGYNLKTALLEVSKTRRATFRFTCNQNLILSDIFPKD
KDLIESILVKFGVHRKTAEVSPIRKNSIACVALNTCSLALAEAQRYLPSLIDKIEPILSKHGLSEEPISIRMTGCPNGCA
RPYISEIGLVGTSYGKYNLHVGADAEGYRLNKKYKEDLDESAILQELDGLFGKFSKDRKGKESFGDYINRIGILK

Sequences:

>Translated_555_residues
MAETKKETLAEKVKRLSRGLRGSLVDSLKDEHTGSLRSDDQLLLKFHGMYQQDDRDRRDERALKKLERLYSFMIRLRIPG
GMIGPVHWEALHNVAGENSTGTIKITTRQTVQLHGILKSKIKPTIKAFDSVFLDSIAACGDVNRNVTCTSNPAASPLHKE
VFGYAGEISRSLLPKTRAYYEIWLDENLLAEKEEPEDPLYKDVYLPRKFKIAIAIPPYNDVDLFTNDIGLIAIIENGQLL
GFNVAVGGGLGTTHGNPDTYPRVGTVFGFIPKKDILRVVYEIVTVQRDFGNREDRKLSRLKYTLDRLGVEFYKREVEKRA
GISFESAKDFQFTTRSDDFGWKQDAAGNWHYTVFVENGRVCDEHGYNLKTALLEVSKTRRATFRFTCNQNLILSDIFPKD
KDLIESILVKFGVHRKTAEVSPIRKNSIACVALNTCSLALAEAQRYLPSLIDKIEPILSKHGLSEEPISIRMTGCPNGCA
RPYISEIGLVGTSYGKYNLHVGADAEGYRLNKKYKEDLDESAILQELDGLFGKFSKDRKGKESFGDYINRIGILK
>Mature_554_residues
AETKKETLAEKVKRLSRGLRGSLVDSLKDEHTGSLRSDDQLLLKFHGMYQQDDRDRRDERALKKLERLYSFMIRLRIPGG
MIGPVHWEALHNVAGENSTGTIKITTRQTVQLHGILKSKIKPTIKAFDSVFLDSIAACGDVNRNVTCTSNPAASPLHKEV
FGYAGEISRSLLPKTRAYYEIWLDENLLAEKEEPEDPLYKDVYLPRKFKIAIAIPPYNDVDLFTNDIGLIAIIENGQLLG
FNVAVGGGLGTTHGNPDTYPRVGTVFGFIPKKDILRVVYEIVTVQRDFGNREDRKLSRLKYTLDRLGVEFYKREVEKRAG
ISFESAKDFQFTTRSDDFGWKQDAAGNWHYTVFVENGRVCDEHGYNLKTALLEVSKTRRATFRFTCNQNLILSDIFPKDK
DLIESILVKFGVHRKTAEVSPIRKNSIACVALNTCSLALAEAQRYLPSLIDKIEPILSKHGLSEEPISIRMTGCPNGCAR
PYISEIGLVGTSYGKYNLHVGADAEGYRLNKKYKEDLDESAILQELDGLFGKFSKDRKGKESFGDYINRIGILK

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=551, Percent_Identity=49.3647912885662, Blast_Score=566, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6322597, Length=583, Percent_Identity=41.6809605488851, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_LEPBA (B0SF75)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962240.1
- ProteinModelPortal:   B0SF75
- SMR:   B0SF75
- GeneID:   6387410
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1138
- HOGENOM:   HBG626671
- OMA:   TRQAFQM
- ProtClustDB:   CLSK2336526
- BioCyc:   LBIF355278:LBF_1138-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 62617; Mature: 62486

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETKKETLAEKVKRLSRGLRGSLVDSLKDEHTGSLRSDDQLLLKFHGMYQQDDRDRRDE
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
RALKKLERLYSFMIRLRIPGGMIGPVHWEALHNVAGENSTGTIKITTRQTVQLHGILKSK
HHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCEEHHHHHHHHH
IKPTIKAFDSVFLDSIAACGDVNRNVTCTSNPAASPLHKEVFGYAGEISRSLLPKTRAYY
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
EIWLDENLLAEKEEPEDPLYKDVYLPRKFKIAIAIPPYNDVDLFTNDIGLIAIIENGQLL
EEEECCCHHCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCCCEEECCCEEEEEEECCEEE
GFNVAVGGGLGTTHGNPDTYPRVGTVFGFIPKKDILRVVYEIVTVQRDFGNREDRKLSRL
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KYTLDRLGVEFYKREVEKRAGISFESAKDFQFTTRSDDFGWKQDAAGNWHYTVFVENGRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCEE
CDEHGYNLKTALLEVSKTRRATFRFTCNQNLILSDIFPKDKDLIESILVKFGVHRKTAEV
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
SPIRKNSIACVALNTCSLALAEAQRYLPSLIDKIEPILSKHGLSEEPISIRMTGCPNGCA
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
RPYISEIGLVGTSYGKYNLHVGADAEGYRLNKKYKEDLDESAILQELDGLFGKFSKDRKG
CHHHHHHCCEECCCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCC
KESFGDYINRIGILK
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
AETKKETLAEKVKRLSRGLRGSLVDSLKDEHTGSLRSDDQLLLKFHGMYQQDDRDRRDE
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
RALKKLERLYSFMIRLRIPGGMIGPVHWEALHNVAGENSTGTIKITTRQTVQLHGILKSK
HHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCEEHHHHHHHHH
IKPTIKAFDSVFLDSIAACGDVNRNVTCTSNPAASPLHKEVFGYAGEISRSLLPKTRAYY
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
EIWLDENLLAEKEEPEDPLYKDVYLPRKFKIAIAIPPYNDVDLFTNDIGLIAIIENGQLL
EEEECCCHHCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCCCEEECCCEEEEEEECCEEE
GFNVAVGGGLGTTHGNPDTYPRVGTVFGFIPKKDILRVVYEIVTVQRDFGNREDRKLSRL
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KYTLDRLGVEFYKREVEKRAGISFESAKDFQFTTRSDDFGWKQDAAGNWHYTVFVENGRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCEE
CDEHGYNLKTALLEVSKTRRATFRFTCNQNLILSDIFPKDKDLIESILVKFGVHRKTAEV
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
SPIRKNSIACVALNTCSLALAEAQRYLPSLIDKIEPILSKHGLSEEPISIRMTGCPNGCA
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
RPYISEIGLVGTSYGKYNLHVGADAEGYRLNKKYKEDLDESAILQELDGLFGKFSKDRKG
CHHHHHHCCEECCCCEEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCC
KESFGDYINRIGILK
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA