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The map label for this gene is rho [H]

Identifier: 183220435

GI number: 183220435

Start: 1073886

End: 1075289

Strand: Direct

Name: rho [H]

Synonym: LEPBI_I1032

Alternate gene names: 183220435

Gene position: 1073886-1075289 (Clockwise)

Preceding gene: 183220434

Following gene: 183220436

Centisome position: 29.83

GC content: 45.16

Gene sequence:

>1404_bases
ATGGCATCACGCAAACAAGAAGAAATCCAAGTCAATCCCCCTGAAGAACCAACTGAATACACAAACGGCATCATGGACCA
AGACGATGCCCAAGAGCCACCGAAACAGTTTAAAAAGAAAAAGAACCGATACGAAGGTCCTATTCCTCCTCCACTTGATT
TAGTCGAACTCAAGAAAAAAAACATCAACGAACTGGCAGACCTCGCGAAAGGTTTGGGAGTAGAGAACACTCATGGTTTG
AAAAAACAAAACTTGATGTTTGCACTCCTCCAAGCGCAAACCGAAAAAGATGGTCAAGTGCACGCAGCGGGAGTCATGGA
AAGGCTTCCTGATGGATATGGTTTCCTTCGTTCACCTGACTACAATTATGTGCCAGGTCCGGATGATATTTATGTTTCTC
CTTCTCAAATCAAATTGTTTGGCCTTCGCACAGGGGATACGGTAACAGGCCTTATTCGTCCTCCCAAAGAAGCAGAACGA
TTTTTTGCAATGTTACGTGTGGAATCCATCAATGGTTTCCCAGTGGAAGTTGCACAAAAAAGAAATTTATTTGATAACCT
AACACCACTTTACCCAAGCGAAAGGATCAATATGGAGTTTGATCCAAGCCATCTGGACACTCGAGTGATTGATCTTATGT
GCCCGATTGGAAAGGGACAAAGGGCACTCATTGTGGCGCCACCGAGAACTGGGAAAACAGTTCTCATGCAATCCATTGCC
AATGCGATAACAAGGAACCACCCAGAAATTTTTCTCATCGTTTTACTCATTGATGAACGTCCTGAAGAAGTAACCGACAT
GGCGCGTCATGTAAAAGGTGAAGTTGTGAGTTCTACGTTTGATGAACCAGCACAACGCCACGTCCAAGTTGCTGAGATGG
TCATTGAAAAAGCAAAACGACTCGTGGAACATGGAAAGGATGTGGTCATCCTCCTCGATTCCATCACAAGGCTTGCCCGT
GCATACAACCAAGTGGTTCCGACTTCAGGAAAGATCCTTTCTGGTGGTGTGGACTCCAATGCCCTCCACAAACCAAAACG
ATTTTTTGGTGCCGCAAGGAATATCGAAGAGGGTGGGTCTCTTACCATCATTGCAACAGCCCTCATTGACACAGGGTCTA
GAATGGACGAAGTGATTTTTGAGGAATTTAAGGGAACGGGAAATATGGAAATCCATTTGGACCGAAAACTCGCGGACAAA
CGAATTTTCCCTGCCATCGACATCAACCGCTCAGGGACCAGGAAGGAAGAACTCCTCCTCCCACAAGATACGCTCACTCG
TGTCTTTATCCTCCGAAAAGTACTTTCTCCTATGAGTATCACCGAAAGTATGGAACTATTGATTGAAAAAATGCGTGGCG
CGAAGACAAACGACCAATTCCTCGCCAGCATGAATACGAACTAA

Upstream 100 bases:

>100_bases
AAACACCTCCGCACTTCGCTGTTTCACTTGCATACATAACTTGCACAAATGGTTTCCCGAATTTTATCAAATACTCTAAC
AAATCAATGTAGAACAATCT

Downstream 100 bases:

>100_bases
AAGGGCCACCATGAAAACTGACATCCATCCAAAATACGTTGCTGCCAAAATCAAATGTGCTTGTGGTACTGTGATTGAAA
CACGTTCCACTTCCGGGGAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MASRKQEEIQVNPPEEPTEYTNGIMDQDDAQEPPKQFKKKKNRYEGPIPPPLDLVELKKKNINELADLAKGLGVENTHGL
KKQNLMFALLQAQTEKDGQVHAAGVMERLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVTGLIRPPKEAER
FFAMLRVESINGFPVEVAQKRNLFDNLTPLYPSERINMEFDPSHLDTRVIDLMCPIGKGQRALIVAPPRTGKTVLMQSIA
NAITRNHPEIFLIVLLIDERPEEVTDMARHVKGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLAR
AYNQVVPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLADK
RIFPAIDINRSGTRKEELLLPQDTLTRVFILRKVLSPMSITESMELLIEKMRGAKTNDQFLASMNTN

Sequences:

>Translated_467_residues
MASRKQEEIQVNPPEEPTEYTNGIMDQDDAQEPPKQFKKKKNRYEGPIPPPLDLVELKKKNINELADLAKGLGVENTHGL
KKQNLMFALLQAQTEKDGQVHAAGVMERLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVTGLIRPPKEAER
FFAMLRVESINGFPVEVAQKRNLFDNLTPLYPSERINMEFDPSHLDTRVIDLMCPIGKGQRALIVAPPRTGKTVLMQSIA
NAITRNHPEIFLIVLLIDERPEEVTDMARHVKGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLAR
AYNQVVPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLADK
RIFPAIDINRSGTRKEELLLPQDTLTRVFILRKVLSPMSITESMELLIEKMRGAKTNDQFLASMNTN
>Mature_466_residues
ASRKQEEIQVNPPEEPTEYTNGIMDQDDAQEPPKQFKKKKNRYEGPIPPPLDLVELKKKNINELADLAKGLGVENTHGLK
KQNLMFALLQAQTEKDGQVHAAGVMERLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVTGLIRPPKEAERF
FAMLRVESINGFPVEVAQKRNLFDNLTPLYPSERINMEFDPSHLDTRVIDLMCPIGKGQRALIVAPPRTGKTVLMQSIAN
AITRNHPEIFLIVLLIDERPEEVTDMARHVKGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARA
YNQVVPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLADKR
IFPAIDINRSGTRKEELLLPQDTLTRVFILRKVLSPMSITESMELLIEKMRGAKTNDQFLASMNTN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=63.7019230769231, Blast_Score=546, Evalue=1e-156,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 52354; Mature: 52223

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRKQEEIQVNPPEEPTEYTNGIMDQDDAQEPPKQFKKKKNRYEGPIPPPLDLVELKKK
CCCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHC
NINELADLAKGLGVENTHGLKKQNLMFALLQAQTEKDGQVHAAGVMERLPDGYGFLRSPD
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
YNYVPGPDDIYVSPSQIKLFGLRTGDTVTGLIRPPKEAERFFAMLRVESINGFPVEVAQK
CCCCCCCCCEEECCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
RNLFDNLTPLYPSERINMEFDPSHLDTRVIDLMCPIGKGQRALIVAPPRTGKTVLMQSIA
HHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
NAITRNHPEIFLIVLLIDERPEEVTDMARHVKGEVVSSTFDEPAQRHVQVAEMVIEKAKR
HHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LVEHGKDVVILLDSITRLARAYNQVVPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGS
HHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCHHHHHHHCCCCCCCC
LTIIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLADKRIFPAIDINRSGTRKEELLL
EEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCEEECCCCCCCHHHEEC
PQDTLTRVFILRKVLSPMSITESMELLIEKMRGAKTNDQFLASMNTN
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure 
ASRKQEEIQVNPPEEPTEYTNGIMDQDDAQEPPKQFKKKKNRYEGPIPPPLDLVELKKK
CCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHC
NINELADLAKGLGVENTHGLKKQNLMFALLQAQTEKDGQVHAAGVMERLPDGYGFLRSPD
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
YNYVPGPDDIYVSPSQIKLFGLRTGDTVTGLIRPPKEAERFFAMLRVESINGFPVEVAQK
CCCCCCCCCEEECCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
RNLFDNLTPLYPSERINMEFDPSHLDTRVIDLMCPIGKGQRALIVAPPRTGKTVLMQSIA
HHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
NAITRNHPEIFLIVLLIDERPEEVTDMARHVKGEVVSSTFDEPAQRHVQVAEMVIEKAKR
HHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LVEHGKDVVILLDSITRLARAYNQVVPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGS
HHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCHHHHHHHCCCCCCCC
LTIIATALIDTGSRMDEVIFEEFKGTGNMEIHLDRKLADKRIFPAIDINRSGTRKEELLL
EEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCEEECCCCCCCHHHEEC
PQDTLTRVFILRKVLSPMSITESMELLIEKMRGAKTNDQFLASMNTN
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]