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The map label for this gene is pyrB

Identifier: 183220429

GI number: 183220429

Start: 1068303

End: 1069235

Strand: Direct

Name: pyrB

Synonym: LEPBI_I1026

Alternate gene names: 183220429

Gene position: 1068303-1069235 (Clockwise)

Preceding gene: 183220427

Following gene: 183220430

Centisome position: 29.68

GC content: 45.55

Gene sequence:

>933_bases
ATGTACGCATACTCCCATAAAAACATCTTAGACACCTTACAATTTTCCAAAGACGACCTCAATTACCTCATAACAAAAAC
AAACCGAATGAATGCCTTGCATGAATCGGGAAAGGCCTTTGGAATCCTTCATGGAAAACTCCTGGCTTCCCTCTTTTTTG
AAGCCAGCACCCGCACTCGGATGAGTTTTGAAGCCGCCATGGAAAGGCTTGGGGGTAGGCTTATCTCCACGGTCGGTTTC
CAGTTTTCTTCCATCTCCAAAGGGGAAACTTTGTATGATACGATGAAAATGATCGAAGCCTATTGCGACATTGCCGTCAT
CCGCCACCCAGTGGAAGGTTCGTCTCGGATTGCAGCAGGGGCAGTGAACATCCCCGTGATCAATGCAGGTGACGGAGCAG
GCCAACACCCCACACAAGCTCTCCTCGATTTGTATACGATTGTTTCTGAAAAAGGAAAGATCGATGGACTGAACATTGCC
TTTATTGGGGATCTGAAGTATGGGCGAACCATTCATTCTCTCATCAATCTCCTCAGACATTACCCAGTGCATTTGTATCT
CATTAGTCCCGAAGAGCTAAGACTCCCCGAAAAGTACAAAAAGAACTTAGAAGGATTTCCAATGACATGGGAGGAAACCA
CTGACATCAAAGCAATTTGGGATGCGGATGTTGCCTATGTCACAAGGATCCAAGAAGAAAGGTTCCCTGACCACAGGGAG
TATGAAAAACTAAAAGATATTTATAAGGTGAATAAGGAACTGGTCCTTGCTTCCAAAAAAGACACAACCATCCTCCACCC
ACTGCCACGCGTGAACGAACTATCCACTGATGTGGATGATTTGCCTAATGCAGCTTACTTTCGTCAGGCGAAGTATGGCG
TTGTGGTGCGAATGGCATTACTTTGTCTGAGCCTTGGAGTGAATTTTGACTAA

Upstream 100 bases:

>100_bases
TATCCCGGAGTTTGTTCTGAATTGTCTCGACCATAAATACCAAAAAATATGTTTTCGATTTATTCGATACCAAAAACCCT
AGAGAAAAGATTTCCGCCTT

Downstream 100 bases:

>100_bases
AAAAATTTGGACTTTGGAAGAAGCAAGAGAAGTCCTTCCACTCGTGCGAGACATTACCAAGGAATATTATCTGAAAGCCA
GTGTGCTTGCAGATGATGTT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MYAYSHKNILDTLQFSKDDLNYLITKTNRMNALHESGKAFGILHGKLLASLFFEASTRTRMSFEAAMERLGGRLISTVGF
QFSSISKGETLYDTMKMIEAYCDIAVIRHPVEGSSRIAAGAVNIPVINAGDGAGQHPTQALLDLYTIVSEKGKIDGLNIA
FIGDLKYGRTIHSLINLLRHYPVHLYLISPEELRLPEKYKKNLEGFPMTWEETTDIKAIWDADVAYVTRIQEERFPDHRE
YEKLKDIYKVNKELVLASKKDTTILHPLPRVNELSTDVDDLPNAAYFRQAKYGVVVRMALLCLSLGVNFD

Sequences:

>Translated_310_residues
MYAYSHKNILDTLQFSKDDLNYLITKTNRMNALHESGKAFGILHGKLLASLFFEASTRTRMSFEAAMERLGGRLISTVGF
QFSSISKGETLYDTMKMIEAYCDIAVIRHPVEGSSRIAAGAVNIPVINAGDGAGQHPTQALLDLYTIVSEKGKIDGLNIA
FIGDLKYGRTIHSLINLLRHYPVHLYLISPEELRLPEKYKKNLEGFPMTWEETTDIKAIWDADVAYVTRIQEERFPDHRE
YEKLKDIYKVNKELVLASKKDTTILHPLPRVNELSTDVDDLPNAAYFRQAKYGVVVRMALLCLSLGVNFD
>Mature_310_residues
MYAYSHKNILDTLQFSKDDLNYLITKTNRMNALHESGKAFGILHGKLLASLFFEASTRTRMSFEAAMERLGGRLISTVGF
QFSSISKGETLYDTMKMIEAYCDIAVIRHPVEGSSRIAAGAVNIPVINAGDGAGQHPTQALLDLYTIVSEKGKIDGLNIA
FIGDLKYGRTIHSLINLLRHYPVHLYLISPEELRLPEKYKKNLEGFPMTWEETTDIKAIWDADVAYVTRIQEERFPDHRE
YEKLKDIYKVNKELVLASKKDTTILHPLPRVNELSTDVDDLPNAAYFRQAKYGVVVRMALLCLSLGVNFD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=43.3224755700326, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI38788445, Length=315, Percent_Identity=25.3968253968254, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=42.671009771987, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1790703, Length=304, Percent_Identity=26.6447368421053, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=43.4375, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=44.8051948051948, Blast_Score=269, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24642586, Length=302, Percent_Identity=44.3708609271523, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_LEPBA (B0SEH6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962097.1
- ProteinModelPortal:   B0SEH6
- SMR:   B0SEH6
- GeneID:   6389761
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0992
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- ProtClustDB:   PRK00856
- BioCyc:   LBIF355278:LBF_0992-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35051; Mature: 35051

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAYSHKNILDTLQFSKDDLNYLITKTNRMNALHESGKAFGILHGKLLASLFFEASTRTR
CCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHH
MSFEAAMERLGGRLISTVGFQFSSISKGETLYDTMKMIEAYCDIAVIRHPVEGSSRIAAG
HHHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
AVNIPVINAGDGAGQHPTQALLDLYTIVSEKGKIDGLNIAFIGDLKYGRTIHSLINLLRH
EEECEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHC
YPVHLYLISPEELRLPEKYKKNLEGFPMTWEETTDIKAIWDADVAYVTRIQEERFPDHRE
CCEEEEEECHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHH
YEKLKDIYKVNKELVLASKKDTTILHPLPRVNELSTDVDDLPNAAYFRQAKYGVVVRMAL
HHHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHH
LCLSLGVNFD
HHHHHCCCCC
>Mature Secondary Structure
MYAYSHKNILDTLQFSKDDLNYLITKTNRMNALHESGKAFGILHGKLLASLFFEASTRTR
CCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCHHH
MSFEAAMERLGGRLISTVGFQFSSISKGETLYDTMKMIEAYCDIAVIRHPVEGSSRIAAG
HHHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
AVNIPVINAGDGAGQHPTQALLDLYTIVSEKGKIDGLNIAFIGDLKYGRTIHSLINLLRH
EEECEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHC
YPVHLYLISPEELRLPEKYKKNLEGFPMTWEETTDIKAIWDADVAYVTRIQEERFPDHRE
CCEEEEEECHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHH
YEKLKDIYKVNKELVLASKKDTTILHPLPRVNELSTDVDDLPNAAYFRQAKYGVVVRMAL
HHHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHH
LCLSLGVNFD
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA