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The map label for this gene is glyA

Identifier: 183220410

GI number: 183220410

Start: 1051467

End: 1052717

Strand: Reverse

Name: glyA

Synonym: LEPBI_I1006

Alternate gene names: 183220410

Gene position: 1052717-1051467 (Counterclockwise)

Preceding gene: 183220411

Following gene: 183220408

Centisome position: 29.24

GC content: 43.17

Gene sequence:

>1251_bases
ATGAGTTATTTAGAAAAACAAGATCCCGAAGTTTACGCCGCTTTAAAAAAAGAAGACGAAAGACAAGAACATTCCCTCGA
AATGATTGCGAGTGAAAATTTTGTTTCGCGTCCTGTTCTTGAAGCTTATCATTCTACACTGACCAATAAATACGCGGAAG
GGTATCCTGGAAAACGTTACTACAATGGTTGCGAAAATGCTGACAGAGTGGAAGAACTCGCCATTGAAAGAGCCAAAAAA
ATGTTTGGTGCTGAATATGCAAATGTCCAACCACATAGCGGTGCTCAGGCGAATATGGCAGTTTTCCTTGCAACCCTTGA
ACCAGGAGATAGTTTCCTTGGAATGAATTTGGCACATGGCGGCCACCTAACACACGGTAGTGCGGTAAACATCAGCGGAA
AGTATTTTAAACCAATCCCATATGGAGTGGATGAAAAAACAGAAACCATCAATTACGATGAAGTGGCAAAACTAGCAAAA
GAACACAAACCAAAACTCATCGTTGTGGGTGCATCAGCTTATCCTCGTGTCATAGATTTTAACAAATTTAGAGAAATCGC
AGATGGAATTGGTGCCAAAATCATGGCAGACATTGCACATATCTCTGGTTTGGTGGTAGCTGGTGAACACCCAAGTCCCA
TTGGAGTTTGTGATTTTGTCACTACCACAACGCATAAAACTCTACGCGGACCAAGAGGAGGACTGATTCTATCTTCTTCC
GAACATGAAAAGATTTTGAATTCGAGGGTGTTCCCTGGAATCCAAGGTGGACCGCTCATGCATGTGATTGCAGCAAAAGC
AGTGGCCTTCGGAGAAGCCCTCCAACCAGATTTCAAAACCTACATCAAACAAGTGGTAAAAAATGCAAAAACCCTTGCGG
AAGTGTTCCAAAAACGTGGATTCCGAGTGGTTTCTGGGGGAACGGACAACCACATCGTTTTACTTGATGTTTCAGTGAAA
GGACTCACAGGAAAAGATGCAGCCGATGGCCTCGATCATATCGGTGTAACGGTAAACAAAAACGCAATCCCTTTTGATAA
AAATCCACCCGCAGTGGCATCAGGGATCCGACTCGGAACCCCTGCTCTCACCACAAGAGGACTCAAAGAAAAGGAAATCG
AAGCCGTTGGCAATTTGATTTGTGATTACTTAGAACATTTTGGTGATACAAGTTTTGAATCCAAAGTGAAGGCAGCTGTG
AAAGAAATCACAGGAGCTTTCCCAATGAACCATTTCCGATTGGAAGACTGA

Upstream 100 bases:

>100_bases
ACTTTTCTACTTTTCGGAAACAAGTGCTCAGAGAATCTGAATCTATTCGCAAGAAGAAATACCAAAATCCAGAATACACA
CTCCATAGAGAAATTCCGAC

Downstream 100 bases:

>100_bases
TCCTTTAGAATTTCAATACCTCTCTCCGTTCCGTATATCCATCTGATAACGGATCGGAGAATGACCTTTCGATCATCTCC
ACAGATTCTTTTCCATAAAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSYLEKQDPEVYAALKKEDERQEHSLEMIASENFVSRPVLEAYHSTLTNKYAEGYPGKRYYNGCENADRVEELAIERAKK
MFGAEYANVQPHSGAQANMAVFLATLEPGDSFLGMNLAHGGHLTHGSAVNISGKYFKPIPYGVDEKTETINYDEVAKLAK
EHKPKLIVVGASAYPRVIDFNKFREIADGIGAKIMADIAHISGLVVAGEHPSPIGVCDFVTTTTHKTLRGPRGGLILSSS
EHEKILNSRVFPGIQGGPLMHVIAAKAVAFGEALQPDFKTYIKQVVKNAKTLAEVFQKRGFRVVSGGTDNHIVLLDVSVK
GLTGKDAADGLDHIGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKEKEIEAVGNLICDYLEHFGDTSFESKVKAAV
KEITGAFPMNHFRLED

Sequences:

>Translated_416_residues
MSYLEKQDPEVYAALKKEDERQEHSLEMIASENFVSRPVLEAYHSTLTNKYAEGYPGKRYYNGCENADRVEELAIERAKK
MFGAEYANVQPHSGAQANMAVFLATLEPGDSFLGMNLAHGGHLTHGSAVNISGKYFKPIPYGVDEKTETINYDEVAKLAK
EHKPKLIVVGASAYPRVIDFNKFREIADGIGAKIMADIAHISGLVVAGEHPSPIGVCDFVTTTTHKTLRGPRGGLILSSS
EHEKILNSRVFPGIQGGPLMHVIAAKAVAFGEALQPDFKTYIKQVVKNAKTLAEVFQKRGFRVVSGGTDNHIVLLDVSVK
GLTGKDAADGLDHIGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKEKEIEAVGNLICDYLEHFGDTSFESKVKAAV
KEITGAFPMNHFRLED
>Mature_415_residues
SYLEKQDPEVYAALKKEDERQEHSLEMIASENFVSRPVLEAYHSTLTNKYAEGYPGKRYYNGCENADRVEELAIERAKKM
FGAEYANVQPHSGAQANMAVFLATLEPGDSFLGMNLAHGGHLTHGSAVNISGKYFKPIPYGVDEKTETINYDEVAKLAKE
HKPKLIVVGASAYPRVIDFNKFREIADGIGAKIMADIAHISGLVVAGEHPSPIGVCDFVTTTTHKTLRGPRGGLILSSSE
HEKILNSRVFPGIQGGPLMHVIAAKAVAFGEALQPDFKTYIKQVVKNAKTLAEVFQKRGFRVVSGGTDNHIVLLDVSVKG
LTGKDAADGLDHIGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKEKEIEAVGNLICDYLEHFGDTSFESKVKAAVK
EITGAFPMNHFRLED

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=420, Percent_Identity=44.2857142857143, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI261862352, Length=420, Percent_Identity=44.2857142857143, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI261862350, Length=420, Percent_Identity=44.2857142857143, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI261862348, Length=420, Percent_Identity=44.2857142857143, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI261862346, Length=420, Percent_Identity=43.3333333333333, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547186, Length=424, Percent_Identity=44.3396226415094, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI22547189, Length=409, Percent_Identity=42.7872860635697, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=58.2716049382716, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=46.1538461538462, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=46.1538461538462, Blast_Score=342, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=397, Percent_Identity=45.088161209068, Blast_Score=344, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=439, Percent_Identity=42.369020501139, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24640005, Length=403, Percent_Identity=47.8908188585608, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=403, Percent_Identity=47.8908188585608, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_LEPBA (B0SEF8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962079.1
- ProteinModelPortal:   B0SEF8
- SMR:   B0SEF8
- GeneID:   6388341
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0974
- HOGENOM:   HBG301263
- OMA:   LNGNRMF
- ProtClustDB:   PRK00011
- BioCyc:   LBIF355278:LBF_0974-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45246; Mature: 45115

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLEKQDPEVYAALKKEDERQEHSLEMIASENFVSRPVLEAYHSTLTNKYAEGYPGKRY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCENADRVEELAIERAKKMFGAEYANVQPHSGAQANMAVFLATLEPGDSFLGMNLAHG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECEEECCC
GHLTHGSAVNISGKYFKPIPYGVDEKTETINYDEVAKLAKEHKPKLIVVGASAYPRVIDF
CCCCCCCEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCH
NKFREIADGIGAKIMADIAHISGLVVAGEHPSPIGVCDFVTTTTHKTLRGPRGGLILSSS
HHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCEEEECC
EHEKILNSRVFPGIQGGPLMHVIAAKAVAFGEALQPDFKTYIKQVVKNAKTLAEVFQKRG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
FRVVSGGTDNHIVLLDVSVKGLTGKDAADGLDHIGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCCHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKEKEIEAVGNLICDYLEHFGDTSFESKVKAAVKEITGAFPMNHFRLED
CHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure 
SYLEKQDPEVYAALKKEDERQEHSLEMIASENFVSRPVLEAYHSTLTNKYAEGYPGKRY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCENADRVEELAIERAKKMFGAEYANVQPHSGAQANMAVFLATLEPGDSFLGMNLAHG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECEEECCC
GHLTHGSAVNISGKYFKPIPYGVDEKTETINYDEVAKLAKEHKPKLIVVGASAYPRVIDF
CCCCCCCEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCH
NKFREIADGIGAKIMADIAHISGLVVAGEHPSPIGVCDFVTTTTHKTLRGPRGGLILSSS
HHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCEEEECC
EHEKILNSRVFPGIQGGPLMHVIAAKAVAFGEALQPDFKTYIKQVVKNAKTLAEVFQKRG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
FRVVSGGTDNHIVLLDVSVKGLTGKDAADGLDHIGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCCHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKEKEIEAVGNLICDYLEHFGDTSFESKVKAAVKEITGAFPMNHFRLED
CHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA