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The map label for this gene is lplA [H]

Identifier: 183220411

GI number: 183220411

Start: 1052714

End: 1053583

Strand: Reverse

Name: lplA [H]

Synonym: LEPBI_I1007

Alternate gene names: 183220411

Gene position: 1053583-1052714 (Counterclockwise)

Preceding gene: 183220412

Following gene: 183220410

Centisome position: 29.27

GC content: 40.69

Gene sequence:

>870_bases
GTGAATCCAAAAGTATTTTTTTTCCCTCCGATCCCTCCAAGATCGCCTTATTATAATTTGGCGGTCGAGGAAGCCATCGC
CGTAAATTTAGTCAACTCACAGATCTCAGCAGGGATCCGATTATGGAAAAATCCAGACTCCATCATCCTTGGGCTTTCTG
AAAACCCATACCGCAATATCAAAGAAGAAGTAGTGAGTGCCTACGAAAAGGAAGTAAACGAAGTGGGTTTTGGGAAAAAA
CCAAAACCAAACTTTTGTTACATTGCCAGACGTGCTTCTGGTGGAGGGACTGTTTTCCATTCCCTAACTGGTAATATCAA
TTATTCGCTTTATTTTAATTTAGAGACTCGAAAGGAATTATTCCCAGTAAAAGACAGTTATGATCGAATCTTGGGAATCA
TCTCAAAATCATTATCCCACCAAAACATCCAGTCCTTTCCCAAAGGGAAATCCGATCTCGTTTTGGAAAAAGATGGTGTT
TTCAAAAAAATTTCGGGGAATGCACAATTTCGCAAACGTGGATGTATCGTCCAACATGGCACCTTAATTTTAGAAGAGGA
CCTCATCAAACGAGTGGGCGAAGTTTTACACCACCCGCCAGAAGAACCTGATTACCGCAAAGAAAGATCCCACAAGGAGT
TTCTCACATCCTTACCCACGTTTTTTTCGGAAGAAAAATGGGCAGTGGATTTGGTACGAGAAGTGTTCCAATACTTGGGA
GAACCCATTCCCAGTGATTTTTCAAAAATTTCCTTCTTTTCGCAGGACTTTTCTACTTTTCGGAAACAAGTGCTCAGAGA
ATCTGAATCTATTCGCAAGAAGAAATACCAAAATCCAGAATACACACTCCATAGAGAAATTCCGACATGA

Upstream 100 bases:

>100_bases
TGGATGAACTTTTGGAAAAAATTTCGAAAAACGGAATGGAATCTCTTTCTAGAAAAGAACGTAAGTTTTTAAACGAAGCT
AGCCAAAAATACTTCAACGA

Downstream 100 bases:

>100_bases
GTTATTTAGAAAAACAAGATCCCGAAGTTTACGCCGCTTTAAAAAAAGAAGACGAAAGACAAGAACATTCCCTCGAAATG
ATTGCGAGTGAAAATTTTGT

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MNPKVFFFPPIPPRSPYYNLAVEEAIAVNLVNSQISAGIRLWKNPDSIILGLSENPYRNIKEEVVSAYEKEVNEVGFGKK
PKPNFCYIARRASGGGTVFHSLTGNINYSLYFNLETRKELFPVKDSYDRILGIISKSLSHQNIQSFPKGKSDLVLEKDGV
FKKISGNAQFRKRGCIVQHGTLILEEDLIKRVGEVLHHPPEEPDYRKERSHKEFLTSLPTFFSEEKWAVDLVREVFQYLG
EPIPSDFSKISFFSQDFSTFRKQVLRESESIRKKKYQNPEYTLHREIPT

Sequences:

>Translated_289_residues
MNPKVFFFPPIPPRSPYYNLAVEEAIAVNLVNSQISAGIRLWKNPDSIILGLSENPYRNIKEEVVSAYEKEVNEVGFGKK
PKPNFCYIARRASGGGTVFHSLTGNINYSLYFNLETRKELFPVKDSYDRILGIISKSLSHQNIQSFPKGKSDLVLEKDGV
FKKISGNAQFRKRGCIVQHGTLILEEDLIKRVGEVLHHPPEEPDYRKERSHKEFLTSLPTFFSEEKWAVDLVREVFQYLG
EPIPSDFSKISFFSQDFSTFRKQVLRESESIRKKKYQNPEYTLHREIPT
>Mature_289_residues
MNPKVFFFPPIPPRSPYYNLAVEEAIAVNLVNSQISAGIRLWKNPDSIILGLSENPYRNIKEEVVSAYEKEVNEVGFGKK
PKPNFCYIARRASGGGTVFHSLTGNINYSLYFNLETRKELFPVKDSYDRILGIISKSLSHQNIQSFPKGKSDLVLEKDGV
FKKISGNAQFRKRGCIVQHGTLILEEDLIKRVGEVLHHPPEEPDYRKERSHKEFLTSLPTFFSEEKWAVDLVREVFQYLG
EPIPSDFSKISFFSQDFSTFRKQVLRESESIRKKKYQNPEYTLHREIPT

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Escherichia coli, GI1790846, Length=242, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 33353; Mature: 33353

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKVFFFPPIPPRSPYYNLAVEEAIAVNLVNSQISAGIRLWKNPDSIILGLSENPYRNI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCHHHH
KEEVVSAYEKEVNEVGFGKKPKPNFCYIARRASGGGTVFHSLTGNINYSLYFNLETRKEL
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEEEEEEHHHHH
FPVKDSYDRILGIISKSLSHQNIQSFPKGKSDLVLEKDGVFKKISGNAQFRKRGCIVQHG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCHHHHCCCEEECC
TLILEEDLIKRVGEVLHHPPEEPDYRKERSHKEFLTSLPTFFSEEKWAVDLVREVFQYLG
CEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
EPIPSDFSKISFFSQDFSTFRKQVLRESESIRKKKYQNPEYTLHREIPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MNPKVFFFPPIPPRSPYYNLAVEEAIAVNLVNSQISAGIRLWKNPDSIILGLSENPYRNI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCHHHH
KEEVVSAYEKEVNEVGFGKKPKPNFCYIARRASGGGTVFHSLTGNINYSLYFNLETRKEL
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEEEEEEHHHHH
FPVKDSYDRILGIISKSLSHQNIQSFPKGKSDLVLEKDGVFKKISGNAQFRKRGCIVQHG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCHHHHCCCEEECC
TLILEEDLIKRVGEVLHHPPEEPDYRKERSHKEFLTSLPTFFSEEKWAVDLVREVFQYLG
CEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
EPIPSDFSKISFFSQDFSTFRKQVLRESESIRKKKYQNPEYTLHREIPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA