Click here to switch to the map view.

The map label for this gene is tig [H]

Identifier: 183220372

GI number: 183220372

Start: 1003932

End: 1005278

Strand: Direct

Name: tig [H]

Synonym: LEPBI_Ia0966

Alternate gene names: 183220372

Gene position: 1003932-1005278 (Clockwise)

Preceding gene: 183220371

Following gene: 183220373

Centisome position: 27.89

GC content: 40.53

Gene sequence:

>1347_bases
ATGGAATTTACGGCTAAAAAAAATAACAACGCAACTTGTGACCTCAGCATTCAATTTAGCGCTGAAGAAGTCCGCACCGC
CTACGGAAAGGCTTACCAAAATGCAGCTGAAAAAGTAAAAATCCCTGGATTCCGACCAGGAAAAGCTCCACTCAATATGG
TGGAAAAAGTCCTCGGTGACTCCGTGATGGACGATGCAGCAAACATCATGTTAAACCAAGCGATGGCTGACCTATTTGAT
AAATTGGAACACAAACCAATTCGATTGCCACAATTCCAAATGGAAACATTTGATAAAAATACTGGTGCCAAAGCAAAAGC
CACTTACGACACAAAACCAGAAGTCACCTTACCAAAGTTAAAGAAGATCAAAATCCAACCCAAAGAAATTAAAATTTCTG
ATGCGGACATCCAAAAAGAATTGGAAGGCATTCAAAAAAATATGGCACGTAACTCTTTGAAAGAAGAGAGCGAACCTGTG
GAAGCAGCCGACTTACTCGAAATCAATTATAAATTCAAAGAAACAGGAAAGGAATATCCAGAACAAAACCAAACGGGTAA
ATTCCAAATGGGAGCCCCTCAAAATCCACCAGGTTTTGAAACGAATCTCCTTGGGATGAAGTTGAATGAAACAAAAGAGT
TTACCTTCACTTATCCAGACGTGTATCCTGCATCTCCTGAGTCCGCTGGAAAATCGATCATCTACACAGTGACTGTGACA
GCTGTATACAAAGTCACTTATCCTGAAATCAACGACGACTTTGCATCCGAAGTGGATGGATCTGCCAACTTACAAGAGTT
AAAAGATAAAACAAAAAAACAACTCGTTGAAATTTTTGGAAATGCTCTTACCAAACGAGCCACGGATGATGCTTACAACG
AAATCATCAAAGAATCCAAATTCATCATCCCTGAATCACTCATCGTGGAAGAAACAGAAACGGTTTTCAAAAACTTTATG
CGTGAATTTGGCCTTCCTGTGACTTCCCTTTCTGACTACGCAAAACGACTCGGTAAGGAAGAAAAGGAAGTGCGAGAGTC
TTTTTCCAAAGCGGCGGAAAAACGCATCCAAACCTATATTTTGAAGCAAAAAATTGCGGATGACCACAAAATCCAGATTT
CTGAGGAAGAAGTGGAGGCCGGATACGAAAAAGAGGCCACTGCCCAAGGAATTCCTGCCGAAACTCTGAAAAAAGAAGTC
CAAAAACAAAAGGCGGAAACCTACTACCGTGACAAATTCCTGTTTGATAAAATTGACGAGTTTGTATACGCTGAAGTAGA
GAAAAAATCGCCGAAAGCAATTTCAACGGAAGAAGCTGAGAAAATTCTTAGCGGGAAAGAAGAGTAA

Upstream 100 bases:

>100_bases
CAAGGAGATGGTCGCGGGTTCAAGTCCCGTTTCCCGCTAATGAAGGTCTTCTTCTTTTTCTTTCCACTACACTCTCTTTT
TTTACACTGGATATAATACG

Downstream 100 bases:

>100_bases
ACTATGTCCACATTATTACCATATGTTTCAGAACAAACAAGCCGGGGCATCGATCGCATGGATGTCTATTCCAGACTTCT
ACGGGATCGGATTATTTTTT

Product: trigger factor 1

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MEFTAKKNNNATCDLSIQFSAEEVRTAYGKAYQNAAEKVKIPGFRPGKAPLNMVEKVLGDSVMDDAANIMLNQAMADLFD
KLEHKPIRLPQFQMETFDKNTGAKAKATYDTKPEVTLPKLKKIKIQPKEIKISDADIQKELEGIQKNMARNSLKEESEPV
EAADLLEINYKFKETGKEYPEQNQTGKFQMGAPQNPPGFETNLLGMKLNETKEFTFTYPDVYPASPESAGKSIIYTVTVT
AVYKVTYPEINDDFASEVDGSANLQELKDKTKKQLVEIFGNALTKRATDDAYNEIIKESKFIIPESLIVEETETVFKNFM
REFGLPVTSLSDYAKRLGKEEKEVRESFSKAAEKRIQTYILKQKIADDHKIQISEEEVEAGYEKEATAQGIPAETLKKEV
QKQKAETYYRDKFLFDKIDEFVYAEVEKKSPKAISTEEAEKILSGKEE

Sequences:

>Translated_448_residues
MEFTAKKNNNATCDLSIQFSAEEVRTAYGKAYQNAAEKVKIPGFRPGKAPLNMVEKVLGDSVMDDAANIMLNQAMADLFD
KLEHKPIRLPQFQMETFDKNTGAKAKATYDTKPEVTLPKLKKIKIQPKEIKISDADIQKELEGIQKNMARNSLKEESEPV
EAADLLEINYKFKETGKEYPEQNQTGKFQMGAPQNPPGFETNLLGMKLNETKEFTFTYPDVYPASPESAGKSIIYTVTVT
AVYKVTYPEINDDFASEVDGSANLQELKDKTKKQLVEIFGNALTKRATDDAYNEIIKESKFIIPESLIVEETETVFKNFM
REFGLPVTSLSDYAKRLGKEEKEVRESFSKAAEKRIQTYILKQKIADDHKIQISEEEVEAGYEKEATAQGIPAETLKKEV
QKQKAETYYRDKFLFDKIDEFVYAEVEKKSPKAISTEEAEKILSGKEE
>Mature_448_residues
MEFTAKKNNNATCDLSIQFSAEEVRTAYGKAYQNAAEKVKIPGFRPGKAPLNMVEKVLGDSVMDDAANIMLNQAMADLFD
KLEHKPIRLPQFQMETFDKNTGAKAKATYDTKPEVTLPKLKKIKIQPKEIKISDADIQKELEGIQKNMARNSLKEESEPV
EAADLLEINYKFKETGKEYPEQNQTGKFQMGAPQNPPGFETNLLGMKLNETKEFTFTYPDVYPASPESAGKSIIYTVTVT
AVYKVTYPEINDDFASEVDGSANLQELKDKTKKQLVEIFGNALTKRATDDAYNEIIKESKFIIPESLIVEETETVFKNFM
REFGLPVTSLSDYAKRLGKEEKEVRESFSKAAEKRIQTYILKQKIADDHKIQISEEEVEAGYEKEATAQGIPAETLKKEV
QKQKAETYYRDKFLFDKIDEFVYAEVEKKSPKAISTEEAEKILSGKEE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=427, Percent_Identity=24.5901639344262, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50764; Mature: 50764

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTAKKNNNATCDLSIQFSAEEVRTAYGKAYQNAAEKVKIPGFRPGKAPLNMVEKVLGD
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCC
SVMDDAANIMLNQAMADLFDKLEHKPIRLPQFQMETFDKNTGAKAKATYDTKPEVTLPKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCH
KKIKIQPKEIKISDADIQKELEGIQKNMARNSLKEESEPVEAADLLEINYKFKETGKEYP
HEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCHHHHHCCCCC
EQNQTGKFQMGAPQNPPGFETNLLGMKLNETKEFTFTYPDVYPASPESAGKSIIYTVTVT
CCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEEEEE
AVYKVTYPEINDDFASEVDGSANLQELKDKTKKQLVEIFGNALTKRATDDAYNEIIKESK
EEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
FIIPESLIVEETETVFKNFMREFGLPVTSLSDYAKRLGKEEKEVRESFSKAAEKRIQTYI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LKQKIADDHKIQISEEEVEAGYEKEATAQGIPAETLKKEVQKQKAETYYRDKFLFDKIDE
HHHHHCCCCEEEECHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYAEVEKKSPKAISTEEAEKILSGKEE
HHHHHHHCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MEFTAKKNNNATCDLSIQFSAEEVRTAYGKAYQNAAEKVKIPGFRPGKAPLNMVEKVLGD
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCC
SVMDDAANIMLNQAMADLFDKLEHKPIRLPQFQMETFDKNTGAKAKATYDTKPEVTLPKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCH
KKIKIQPKEIKISDADIQKELEGIQKNMARNSLKEESEPVEAADLLEINYKFKETGKEYP
HEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCHHHHHCCCCC
EQNQTGKFQMGAPQNPPGFETNLLGMKLNETKEFTFTYPDVYPASPESAGKSIIYTVTVT
CCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEEEEE
AVYKVTYPEINDDFASEVDGSANLQELKDKTKKQLVEIFGNALTKRATDDAYNEIIKESK
EEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
FIIPESLIVEETETVFKNFMREFGLPVTSLSDYAKRLGKEEKEVRESFSKAAEKRIQTYI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LKQKIADDHKIQISEEEVEAGYEKEATAQGIPAETLKKEVQKQKAETYYRDKFLFDKIDE
HHHHHCCCCEEEECHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYAEVEKKSPKAISTEEAEKILSGKEE
HHHHHHHCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA