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The map label for this gene is hisA

Identifier: 183220281

GI number: 183220281

Start: 894365

End: 895108

Strand: Direct

Name: hisA

Synonym: LEPBI_I0876

Alternate gene names: 183220281

Gene position: 894365-895108 (Clockwise)

Preceding gene: 183220280

Following gene: 183220282

Centisome position: 24.85

GC content: 39.52

Gene sequence:

>744_bases
ATGTTAGTATTACCAGCAATTGATTTACTCGACAACGAAGCTGTCCGCCTTCTACAAGGTGATTATTCCAAAAAAACCGT
TTATTCGTCTTCTCCCGAAAAAATGATCCAAGTCTTTGAAGAACAGGGTGCCACTCTCATCCATATTGTGGATCTCAATG
CGGCAAAAACGGGAAAATCGGAAAATGAAAAAACAATCAAAAAAATCAAAGAGAAGTGCACCGTAGATCTAGAGTTAGGT
GGAGGTATCCGGACCATCGACAATATGAAGTTTTATGATGGATTGGGTGTTTCACGACTGATTTTAGGAACTGTTGCTGT
GGAAGAACCCAATGTCGTAGAAAAGGCAGTTTCTTTATTTGGCCAAGACCGCATTGTCATCGGTGTGGATGCAAAAAATG
GATATGTCAGAACCAAAGGTTGGGAGTCTAATTCTGGAATATTGTACAAAGATTTTTTAACGACCATGTATGGAATGGGA
ATTCGCCACGTGATCTTCACCGATATTGCAAGAGATGGAATGATGGAAGGCCCAAATACTTTAGCGTATGCCGAATTACT
TGAAACATTCCCAGACTTACAACTTGTCGCTTCTGGTGGTGTTTCTTCCAAAGAGGATTTGGTGGAACTCTATGACAAAA
CGAATGGAAAATTGTTTGGTGCCATCACAGGGAAAGCCATTTATGAAGGAAAATTAGATCTAAAAGAAAGTATCAGGATT
CTAAATCAAAAGAGGGAAAAATAG

Upstream 100 bases:

>100_bases
CTGCTGTTGTGGAAAAAGAAACCATATTTGGAACCCAGTTCCACCCTGAAAAGTCAGATAAACTAGGACTTGGAATCCTA
AAGAATTTCATCGAAATCTA

Downstream 100 bases:

>100_bases
AAATGAATCGTAAAAATTTGGCAATTGGTGGACTCGTCGTAGGAGTTTTAGGACTTCTATTTTCGTTCCTATTAGCAGTC
GAGTATTTTGGAATTGGAAC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MLVLPAIDLLDNEAVRLLQGDYSKKTVYSSSPEKMIQVFEEQGATLIHIVDLNAAKTGKSENEKTIKKIKEKCTVDLELG
GGIRTIDNMKFYDGLGVSRLILGTVAVEEPNVVEKAVSLFGQDRIVIGVDAKNGYVRTKGWESNSGILYKDFLTTMYGMG
IRHVIFTDIARDGMMEGPNTLAYAELLETFPDLQLVASGGVSSKEDLVELYDKTNGKLFGAITGKAIYEGKLDLKESIRI
LNQKREK

Sequences:

>Translated_247_residues
MLVLPAIDLLDNEAVRLLQGDYSKKTVYSSSPEKMIQVFEEQGATLIHIVDLNAAKTGKSENEKTIKKIKEKCTVDLELG
GGIRTIDNMKFYDGLGVSRLILGTVAVEEPNVVEKAVSLFGQDRIVIGVDAKNGYVRTKGWESNSGILYKDFLTTMYGMG
IRHVIFTDIARDGMMEGPNTLAYAELLETFPDLQLVASGGVSSKEDLVELYDKTNGKLFGAITGKAIYEGKLDLKESIRI
LNQKREK
>Mature_247_residues
MLVLPAIDLLDNEAVRLLQGDYSKKTVYSSSPEKMIQVFEEQGATLIHIVDLNAAKTGKSENEKTIKKIKEKCTVDLELG
GGIRTIDNMKFYDGLGVSRLILGTVAVEEPNVVEKAVSLFGQDRIVIGVDAKNGYVRTKGWESNSGILYKDFLTTMYGMG
IRHVIFTDIARDGMMEGPNTLAYAELLETFPDLQLVASGGVSSKEDLVELYDKTNGKLFGAITGKAIYEGKLDLKESIRI
LNQKREK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=33.8842975206612, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_LEPBA (B0SDS6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961954.1
- GeneID:   6388976
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0843
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   CLSK573362
- BioCyc:   LBIF355278:LBF_0843-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27274; Mature: 27274

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLPAIDLLDNEAVRLLQGDYSKKTVYSSSPEKMIQVFEEQGATLIHIVDLNAAKTGKS
CEEECCHHHHCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
ENEKTIKKIKEKCTVDLELGGGIRTIDNMKFYDGLGVSRLILGTVAVEEPNVVEKAVSLF
CCHHHHHHHHHHCEEEEEECCCEEEECCCCHHCCCCHHHHHHHHHCCCCCHHHHHHHHHH
GQDRIVIGVDAKNGYVRTKGWESNSGILYKDFLTTMYGMGIRHVIFTDIARDGMMEGPNT
CCCEEEEEEECCCCEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCH
LAYAELLETFPDLQLVASGGVSSKEDLVELYDKTNGKLFGAITGKAIYEGKLDLKESIRI
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEEECCCCCHHHHHHH
LNQKREK
HHHHCCC
>Mature Secondary Structure
MLVLPAIDLLDNEAVRLLQGDYSKKTVYSSSPEKMIQVFEEQGATLIHIVDLNAAKTGKS
CEEECCHHHHCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
ENEKTIKKIKEKCTVDLELGGGIRTIDNMKFYDGLGVSRLILGTVAVEEPNVVEKAVSLF
CCHHHHHHHHHHCEEEEEECCCEEEECCCCHHCCCCHHHHHHHHHCCCCCHHHHHHHHHH
GQDRIVIGVDAKNGYVRTKGWESNSGILYKDFLTTMYGMGIRHVIFTDIARDGMMEGPNT
CCCEEEEEEECCCCEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCH
LAYAELLETFPDLQLVASGGVSSKEDLVELYDKTNGKLFGAITGKAIYEGKLDLKESIRI
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEEECCCCCHHHHHHH
LNQKREK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA