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The map label for this gene is hisH [H]

Identifier: 183220280

GI number: 183220280

Start: 893739

End: 894365

Strand: Direct

Name: hisH [H]

Synonym: LEPBI_I0875

Alternate gene names: 183220280

Gene position: 893739-894365 (Clockwise)

Preceding gene: 183220279

Following gene: 183220281

Centisome position: 24.83

GC content: 38.12

Gene sequence:

>627_bases
GTGATCGCTGTTTTGGATTTTGGAATGGGAAATATCCATTCCTTGTTAAAGGCAGTCTCGCTATATACAAACGATTTTGT
TTTTACGAGTGATCCCGAAAAGGTAAAACTAGCGGATAAAATCATTTTACCAGGTGATGGACATTTTGATAAAGCAATGC
AGAACCTAAACGAACTCGGGTTTACTTCGCTGATCAAAGACCACATTGAAAGAAAAAAGTATCTATTTGGGATTTGTATC
GGCTATCAAGTATTATTCGAGGATTCCGATGAGACAAATAAACCAGGGTCTACAATCTCTGGGCTCGGACTCATTCGAGG
GAAAATCAGAAAGTTTGAAGGCAAAGCAAATCTCAAAGTGCCACATATGGGTTGGAATAAACTCTTTGATATCAAACAAA
AAAATTCCAAATTACTCAAAGGGATTCCAAATGAATCTTTTATGTACTTCATCCATTCTTACCGTCCGGTAGGCGTAGAC
CGATTGGACATCACAGCAAACTGTCATTATTACGGAGAATCTTTCCCTGCTGTTGTGGAAAAAGAAACCATATTTGGAAC
CCAGTTCCACCCTGAAAAGTCAGATAAACTAGGACTTGGAATCCTAAAGAATTTCATCGAAATCTAA

Upstream 100 bases:

>100_bases
ACGAGTCGATTTTTAAGGCTTTGGGAAAGGCACTCCGGCAAGCCATTGCAATCGACACGAGCGCCAAAGACCAAATTCCA
TCCACAAAGGGAATGCTCGA

Downstream 100 bases:

>100_bases
TGTTAGTATTACCAGCAATTGATTTACTCGACAACGAAGCTGTCCGCCTTCTACAAGGTGATTATTCCAAAAAAACCGTT
TATTCGTCTTCTCCCGAAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIAVLDFGMGNIHSLLKAVSLYTNDFVFTSDPEKVKLADKIILPGDGHFDKAMQNLNELGFTSLIKDHIERKKYLFGICI
GYQVLFEDSDETNKPGSTISGLGLIRGKIRKFEGKANLKVPHMGWNKLFDIKQKNSKLLKGIPNESFMYFIHSYRPVGVD
RLDITANCHYYGESFPAVVEKETIFGTQFHPEKSDKLGLGILKNFIEI

Sequences:

>Translated_208_residues
MIAVLDFGMGNIHSLLKAVSLYTNDFVFTSDPEKVKLADKIILPGDGHFDKAMQNLNELGFTSLIKDHIERKKYLFGICI
GYQVLFEDSDETNKPGSTISGLGLIRGKIRKFEGKANLKVPHMGWNKLFDIKQKNSKLLKGIPNESFMYFIHSYRPVGVD
RLDITANCHYYGESFPAVVEKETIFGTQFHPEKSDKLGLGILKNFIEI
>Mature_208_residues
MIAVLDFGMGNIHSLLKAVSLYTNDFVFTSDPEKVKLADKIILPGDGHFDKAMQNLNELGFTSLIKDHIERKKYLFGICI
GYQVLFEDSDETNKPGSTISGLGLIRGKIRKFEGKANLKVPHMGWNKLFDIKQKNSKLLKGIPNESFMYFIHSYRPVGVD
RLDITANCHYYGESFPAVVEKETIFGTQFHPEKSDKLGLGILKNFIEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=36.7149758454106, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=33.4862385321101, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23521; Mature: 23521

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVLDFGMGNIHSLLKAVSLYTNDFVFTSDPEKVKLADKIILPGDGHFDKAMQNLNELG
CEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCC
FTSLIKDHIERKKYLFGICIGYQVLFEDSDETNKPGSTISGLGLIRGKIRKFEGKANLKV
HHHHHHHHHHHHHHHEEHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
PHMGWNKLFDIKQKNSKLLKGIPNESFMYFIHSYRPVGVDRLDITANCHYYGESFPAVVE
CCCCHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCEE
KETIFGTQFHPEKSDKLGLGILKNFIEI
CCEEECCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIAVLDFGMGNIHSLLKAVSLYTNDFVFTSDPEKVKLADKIILPGDGHFDKAMQNLNELG
CEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHCC
FTSLIKDHIERKKYLFGICIGYQVLFEDSDETNKPGSTISGLGLIRGKIRKFEGKANLKV
HHHHHHHHHHHHHHHEEHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEC
PHMGWNKLFDIKQKNSKLLKGIPNESFMYFIHSYRPVGVDRLDITANCHYYGESFPAVVE
CCCCHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCEE
KETIFGTQFHPEKSDKLGLGILKNFIEI
CCEEECCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]