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The map label for this gene is algI [H]

Identifier: 183220220

GI number: 183220220

Start: 827912

End: 829309

Strand: Direct

Name: algI [H]

Synonym: LEPBI_I0812

Alternate gene names: 183220220

Gene position: 827912-829309 (Clockwise)

Preceding gene: 183220219

Following gene: 183220221

Centisome position: 23.0

GC content: 32.62

Gene sequence:

>1398_bases
GTGTTATTCAACTCCCTGACGTTTCTTTTACATTTTTTTGTATTCTACTTAATTTATTATTACTCATCATTTCGGATTCG
AAAACTACTACTGCTCATTTTTGGGATCTATTTTTATTCACAATGGGGAATTGGTGGCACAATTTTACTTTTTGCCTCTA
TTCTTTTTAATTATACGATTGGAATTTTAATCGATCGAAGTTCGTTAGCAAGAAAAAAACACCTATTTGTTTTTGGTATT
GTATCGAATGTTTTGTATCTTGCTGTATTTAAATATTTTTTGTTTGTATGGGGAGTTCTTTCTGATATTAGAATCGAATT
TGGAGAAACTCCAATCCAATGGAAACCCAATATATTACTTCCGATTGGAATCTCCTTTTATACATTTCATAACATTAGTT
ATTTGATTGAAGTGTATGACAAAAGGATAATGGTATGTAAAAATATTTTTACCTTTATCCTTTATGATTTATTTTTTCCA
TTACTTTTGCTTGGACCCATTGAACGACCAGGTAATTTGATTCCACAAATAGAAGCCGAACGTAAGATTTCAAAGGGAAT
GATTTGGAATGGATTGTCTCTTTTTTGTTTTGGTGTGTTCATCAAATCGAGTATTGCGGATCCCTTGTCTCGTTATGTGG
AAATCCACGTCTCTTCCTTTACCAGTTTAGAACCGGGAATTTTATGGATCATTGCCCCTACGATTGCCTTTCAAGTTTAT
GCTGATTTTTTTGGTTATTCCCTTTGTGCAATGGGACTTGCCGAAATGTTGGGATTCGAACTGATGAATAATTTCAAAAG
GCCATTTTTTTCCTCCAACCCTTCGGAGTTTTGGTCAAAATGGCATATCTCACTTTCGACTTGGTTACGTGATTATGTGT
ACATTAAGTTAGGTGGGAATCGACATGGTTTTTTTCGTGAAAACATAAACTTGATGTTAGTTTGGTTTTTGACCGGAATT
TGGCATGGAGCAGGTTATGGTTTTATCATTTGGGGTTTTTATTTAGGTGTTTGCCTGATTCTCTATCGAGTTTTAAAACA
TATTGGACTAACCAAATTCCAAAACAAAATTTTCACTGGATTGGGGATTGTATTTACGTTTTATACTTTTTCTCTTGGAT
TATTATTATTTAGGATTACTGCCCCTTCCGAGAGTTTACTTATATTGAAGAATTTAAAATCGATACCAACATTACAACAA
ATCCCTTTTTCACTTTTTTTTATCATATTCCCATTGTTAGTTTTTGACCTTTGGCAAGAATGGAAACAGACAGAAAGGCC
AACATTTTTTATTTCAACAAAACCTTATTTCGTTTCTGCCATTTTGGTGTTCTGTTTTTTTTGGTTTTCCATTGTATCAC
CTTTTGGAAAAGAAGACTTTTTTTATTTTCAATTTTAA

Upstream 100 bases:

>100_bases
TTTCCGGGCAAGTTACCGTGTATTAACTTTGGCATATCCACATGATACAAAGGACAAAAATCAATTTTTGGAAATGGTTG
AAGAAGCCTAATCAATTCTT

Downstream 100 bases:

>100_bases
AACTAAGTATGTAATCAAATTGTATGAAAATCCGTTTTCTGTTTATCATTTTTTTGACGATATCGATTGTGATGCATTCC
ACGTATGGTTTTTTCATTTC

Product: MBOAT family acyl-CoA thioesterase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MLFNSLTFLLHFFVFYLIYYYSSFRIRKLLLLIFGIYFYSQWGIGGTILLFASILFNYTIGILIDRSSLARKKHLFVFGI
VSNVLYLAVFKYFLFVWGVLSDIRIEFGETPIQWKPNILLPIGISFYTFHNISYLIEVYDKRIMVCKNIFTFILYDLFFP
LLLLGPIERPGNLIPQIEAERKISKGMIWNGLSLFCFGVFIKSSIADPLSRYVEIHVSSFTSLEPGILWIIAPTIAFQVY
ADFFGYSLCAMGLAEMLGFELMNNFKRPFFSSNPSEFWSKWHISLSTWLRDYVYIKLGGNRHGFFRENINLMLVWFLTGI
WHGAGYGFIIWGFYLGVCLILYRVLKHIGLTKFQNKIFTGLGIVFTFYTFSLGLLLFRITAPSESLLILKNLKSIPTLQQ
IPFSLFFIIFPLLVFDLWQEWKQTERPTFFISTKPYFVSAILVFCFFWFSIVSPFGKEDFFYFQF

Sequences:

>Translated_465_residues
MLFNSLTFLLHFFVFYLIYYYSSFRIRKLLLLIFGIYFYSQWGIGGTILLFASILFNYTIGILIDRSSLARKKHLFVFGI
VSNVLYLAVFKYFLFVWGVLSDIRIEFGETPIQWKPNILLPIGISFYTFHNISYLIEVYDKRIMVCKNIFTFILYDLFFP
LLLLGPIERPGNLIPQIEAERKISKGMIWNGLSLFCFGVFIKSSIADPLSRYVEIHVSSFTSLEPGILWIIAPTIAFQVY
ADFFGYSLCAMGLAEMLGFELMNNFKRPFFSSNPSEFWSKWHISLSTWLRDYVYIKLGGNRHGFFRENINLMLVWFLTGI
WHGAGYGFIIWGFYLGVCLILYRVLKHIGLTKFQNKIFTGLGIVFTFYTFSLGLLLFRITAPSESLLILKNLKSIPTLQQ
IPFSLFFIIFPLLVFDLWQEWKQTERPTFFISTKPYFVSAILVFCFFWFSIVSPFGKEDFFYFQF
>Mature_465_residues
MLFNSLTFLLHFFVFYLIYYYSSFRIRKLLLLIFGIYFYSQWGIGGTILLFASILFNYTIGILIDRSSLARKKHLFVFGI
VSNVLYLAVFKYFLFVWGVLSDIRIEFGETPIQWKPNILLPIGISFYTFHNISYLIEVYDKRIMVCKNIFTFILYDLFFP
LLLLGPIERPGNLIPQIEAERKISKGMIWNGLSLFCFGVFIKSSIADPLSRYVEIHVSSFTSLEPGILWIIAPTIAFQVY
ADFFGYSLCAMGLAEMLGFELMNNFKRPFFSSNPSEFWSKWHISLSTWLRDYVYIKLGGNRHGFFRENINLMLVWFLTGI
WHGAGYGFIIWGFYLGVCLILYRVLKHIGLTKFQNKIFTGLGIVFTFYTFSLGLLLFRITAPSESLLILKNLKSIPTLQQ
IPFSLFFIIFPLLVFDLWQEWKQTERPTFFISTKPYFVSAILVFCFFWFSIVSPFGKEDFFYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 54581; Mature: 54581

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSLTFLLHFFVFYLIYYYSSFRIRKLLLLIFGIYFYSQWGIGGTILLFASILFNYTI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GILIDRSSLARKKHLFVFGIVSNVLYLAVFKYFLFVWGVLSDIRIEFGETPIQWKPNILL
HEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
PIGISFYTFHNISYLIEVYDKRIMVCKNIFTFILYDLFFPLLLLGPIERPGNLIPQIEAE
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RKISKGMIWNGLSLFCFGVFIKSSIADPLSRYVEIHVSSFTSLEPGILWIIAPTIAFQVY
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHH
ADFFGYSLCAMGLAEMLGFELMNNFKRPFFSSNPSEFWSKWHISLSTWLRDYVYIKLGGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCC
RHGFFRENINLMLVWFLTGIWHGAGYGFIIWGFYLGVCLILYRVLKHIGLTKFQNKIFTG
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LGIVFTFYTFSLGLLLFRITAPSESLLILKNLKSIPTLQQIPFSLFFIIFPLLVFDLWQE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
WKQTERPTFFISTKPYFVSAILVFCFFWFSIVSPFGKEDFFYFQF
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure
MLFNSLTFLLHFFVFYLIYYYSSFRIRKLLLLIFGIYFYSQWGIGGTILLFASILFNYTI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GILIDRSSLARKKHLFVFGIVSNVLYLAVFKYFLFVWGVLSDIRIEFGETPIQWKPNILL
HEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
PIGISFYTFHNISYLIEVYDKRIMVCKNIFTFILYDLFFPLLLLGPIERPGNLIPQIEAE
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RKISKGMIWNGLSLFCFGVFIKSSIADPLSRYVEIHVSSFTSLEPGILWIIAPTIAFQVY
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHH
ADFFGYSLCAMGLAEMLGFELMNNFKRPFFSSNPSEFWSKWHISLSTWLRDYVYIKLGGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCC
RHGFFRENINLMLVWFLTGIWHGAGYGFIIWGFYLGVCLILYRVLKHIGLTKFQNKIFTG
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LGIVFTFYTFSLGLLLFRITAPSESLLILKNLKSIPTLQQIPFSLFFIIFPLLVFDLWQE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
WKQTERPTFFISTKPYFVSAILVFCFFWFSIVSPFGKEDFFYFQF
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]