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The map label for this gene is amtB1 [H]

Identifier: 183220204

GI number: 183220204

Start: 813204

End: 814493

Strand: Reverse

Name: amtB1 [H]

Synonym: LEPBI_I0794

Alternate gene names: 183220204

Gene position: 814493-813204 (Counterclockwise)

Preceding gene: 183220222

Following gene: 183220203

Centisome position: 22.63

GC content: 40.62

Gene sequence:

>1290_bases
ATGAATGTACAAATGGTCTTACGACCTGTTTTGTTTGGTTTTCTGTTGCTTTTACCAAATATCCTTTTGGCAGAGGGCGA
AACAGGAACGGTCAATCCAATCGATAAATCAGACACTACCTGGATGTTAGTTTCATCTGCTTTGGTCTTTTTTATGATCC
CTGGGCTTTCTTTATTTTATGGCGGAATCGTTCGATCGAAAAATGTTCTATCAACGATGATGCATAGTTTTGTTGCCATG
ATTGTAATGACATTGCAATGGACAATTTTTGGGTATAGCTTTGCATTCTCAGGTGAAAATCCTTATATAGGGAATTTTGA
TTTAGCTTTCTTAGATGGAATCGATTTGAATTCAACAAAAGGATCCATACCTACTTATGTACACTTTTTATTCCAAGGTA
TGTTTGCTCTTATCACTCCTGCTCTCATATCTGGGGCAATCGCCGAAAGAATTAAACTATCTGCTTATATATTATTTATA
CTCGTATGGTCTACGTTAGTGTATGATCCCGTTGCGCATTGGGTTTGGGCAGATTCTGGATGGTTATTCAACATGAATGC
TTTGGATTTTGCAGGGGGAACTGTCGTACATTTAATTTCAGGGATAGCTGGACTCTCCGCTGCAATAGTCATAGGCAAAC
GAAAAGGTGATCCTGGTCTTCTCACTCATCCAAATAACATGACTTATACGCTCCTTGGATCTGGTTTGTTGTGGTTTGGT
TGGTTTGGTTTTAATGCAGGCTCTGGCCTTGCAGTCAATGGTTTAGCAGCAAGAGCATTTTTAGTAACACTAATTGCACC
TGCGGCCGCAGGCGCCAGTTGGTTGTTAATTGAATGGTACCATACAAAAAAAGCAACCGCACTCGGAGCAGCCTCTGGAA
TTGTAGCAGGTCTAGTCGTAATCACACCTGCTTCTGGTTTTGTAGGGATCAAAGGTGCATTGATCATGGGTGTCCTTGTT
TCACCAGTTTGTTATTTGGCAATCCTTCTCAAAGGAAAATTGAAATATGACGATACCTTGGATGCATTTGGAATCCATGG
TGCAGGTGGGGCACTTGGCGCCATTCTAACAGGTATCTTTGCATTAGAATTGGCAGAGGGAATGACATTTGAAAGCCAAA
TGATCGCACAATTGATCAGTGTCATCGCGACTGGTTTTTATTCCTTTGTTGTTTCTTACATACTTGCTCTCATCATTGAG
AGAACAATTGGATTTAGAATTGAAGAAGACAAAGAAATCACGGGCCTCGACCAAGAGATCCATGGTGAAAAAGGATACGA
TATCAGGTAA

Upstream 100 bases:

>100_bases
TTCATGTTTTTACGATTTTTACTCGCTTTCAAACTCTACCCAATTCCTAATAATACGAATTTTATTTCGGAAATCGAATC
TAGTTACTTTGGAGTATACT

Downstream 100 bases:

>100_bases
AAAATATGAAATTAGTAATTGCAATCATACAACCACACAAACTGGAAGAAGTTAAAAATGAACTAACCAAAAACGAAATC
TATCGCCTAACAGTGAGTGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNVQMVLRPVLFGFLLLLPNILLAEGETGTVNPIDKSDTTWMLVSSALVFFMIPGLSLFYGGIVRSKNVLSTMMHSFVAM
IVMTLQWTIFGYSFAFSGENPYIGNFDLAFLDGIDLNSTKGSIPTYVHFLFQGMFALITPALISGAIAERIKLSAYILFI
LVWSTLVYDPVAHWVWADSGWLFNMNALDFAGGTVVHLISGIAGLSAAIVIGKRKGDPGLLTHPNNMTYTLLGSGLLWFG
WFGFNAGSGLAVNGLAARAFLVTLIAPAAAGASWLLIEWYHTKKATALGAASGIVAGLVVITPASGFVGIKGALIMGVLV
SPVCYLAILLKGKLKYDDTLDAFGIHGAGGALGAILTGIFALELAEGMTFESQMIAQLISVIATGFYSFVVSYILALIIE
RTIGFRIEEDKEITGLDQEIHGEKGYDIR

Sequences:

>Translated_429_residues
MNVQMVLRPVLFGFLLLLPNILLAEGETGTVNPIDKSDTTWMLVSSALVFFMIPGLSLFYGGIVRSKNVLSTMMHSFVAM
IVMTLQWTIFGYSFAFSGENPYIGNFDLAFLDGIDLNSTKGSIPTYVHFLFQGMFALITPALISGAIAERIKLSAYILFI
LVWSTLVYDPVAHWVWADSGWLFNMNALDFAGGTVVHLISGIAGLSAAIVIGKRKGDPGLLTHPNNMTYTLLGSGLLWFG
WFGFNAGSGLAVNGLAARAFLVTLIAPAAAGASWLLIEWYHTKKATALGAASGIVAGLVVITPASGFVGIKGALIMGVLV
SPVCYLAILLKGKLKYDDTLDAFGIHGAGGALGAILTGIFALELAEGMTFESQMIAQLISVIATGFYSFVVSYILALIIE
RTIGFRIEEDKEITGLDQEIHGEKGYDIR
>Mature_429_residues
MNVQMVLRPVLFGFLLLLPNILLAEGETGTVNPIDKSDTTWMLVSSALVFFMIPGLSLFYGGIVRSKNVLSTMMHSFVAM
IVMTLQWTIFGYSFAFSGENPYIGNFDLAFLDGIDLNSTKGSIPTYVHFLFQGMFALITPALISGAIAERIKLSAYILFI
LVWSTLVYDPVAHWVWADSGWLFNMNALDFAGGTVVHLISGIAGLSAAIVIGKRKGDPGLLTHPNNMTYTLLGSGLLWFG
WFGFNAGSGLAVNGLAARAFLVTLIAPAAAGASWLLIEWYHTKKATALGAASGIVAGLVVITPASGFVGIKGALIMGVLV
SPVCYLAILLKGKLKYDDTLDAFGIHGAGGALGAILTGIFALELAEGMTFESQMIAQLISVIATGFYSFVVSYILALIIE
RTIGFRIEEDKEITGLDQEIHGEKGYDIR

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=421, Percent_Identity=44.1805225653207, Blast_Score=328, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=30.9468822170901, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=27.2093023255814, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=28.4116331096197, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=27.6887871853547, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325396, Length=411, Percent_Identity=35.7664233576642, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324187, Length=420, Percent_Identity=37.3809523809524, Blast_Score=252, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6321559, Length=424, Percent_Identity=33.2547169811321, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=30.9756097560976, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46005; Mature: 46005

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQMVLRPVLFGFLLLLPNILLAEGETGTVNPIDKSDTTWMLVSSALVFFMIPGLSLFY
CCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GGIVRSKNVLSTMMHSFVAMIVMTLQWTIFGYSFAFSGENPYIGNFDLAFLDGIDLNSTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCCCC
GSIPTYVHFLFQGMFALITPALISGAIAERIKLSAYILFILVWSTLVYDPVAHWVWADSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
WLFNMNALDFAGGTVVHLISGIAGLSAAIVIGKRKGDPGLLTHPNNMTYTLLGSGLLWFG
EEEECCCHHCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEECCCCCEEEEECCCHHEEE
WFGFNAGSGLAVNGLAARAFLVTLIAPAAAGASWLLIEWYHTKKATALGAASGIVAGLVV
EECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHEEEE
ITPASGFVGIKGALIMGVLVSPVCYLAILLKGKLKYDDTLDAFGIHGAGGALGAILTGIF
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHH
ALELAEGMTFESQMIAQLISVIATGFYSFVVSYILALIIERTIGFRIEEDKEITGLDQEI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHH
HGEKGYDIR
CCCCCCCCC
>Mature Secondary Structure
MNVQMVLRPVLFGFLLLLPNILLAEGETGTVNPIDKSDTTWMLVSSALVFFMIPGLSLFY
CCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GGIVRSKNVLSTMMHSFVAMIVMTLQWTIFGYSFAFSGENPYIGNFDLAFLDGIDLNSTK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCCCC
GSIPTYVHFLFQGMFALITPALISGAIAERIKLSAYILFILVWSTLVYDPVAHWVWADSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
WLFNMNALDFAGGTVVHLISGIAGLSAAIVIGKRKGDPGLLTHPNNMTYTLLGSGLLWFG
EEEECCCHHCCCCHHHHHHHHHHHHHEEEEEEECCCCCCCEECCCCCEEEEECCCHHEEE
WFGFNAGSGLAVNGLAARAFLVTLIAPAAAGASWLLIEWYHTKKATALGAASGIVAGLVV
EECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHEEEE
ITPASGFVGIKGALIMGVLVSPVCYLAILLKGKLKYDDTLDAFGIHGAGGALGAILTGIF
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHH
ALELAEGMTFESQMIAQLISVIATGFYSFVVSYILALIIERTIGFRIEEDKEITGLDQEI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHH
HGEKGYDIR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]