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The map label for this gene is glnB [H]

Identifier: 183220203

GI number: 183220203

Start: 812851

End: 813198

Strand: Reverse

Name: glnB [H]

Synonym: LEPBI_I0793

Alternate gene names: 183220203

Gene position: 813198-812851 (Counterclockwise)

Preceding gene: 183220204

Following gene: 183220199

Centisome position: 22.59

GC content: 39.37

Gene sequence:

>348_bases
ATGAAATTAGTAATTGCAATCATACAACCACACAAACTGGAAGAAGTTAAAAATGAACTAACCAAAAACGAAATCTATCG
CCTAACAGTGAGTGATGTCCAAGGGTATGGCCAACAAAAAGGGAAAACAGAAGTATTCCGTGGACATGAATACCAAGTCA
ACCTACTTAGAAAAGTACGGTTGGAAATTGCTGTGAATGATGAATTCGTAAAACCCACTGTAGATGCCATTTTAAAGGCC
GCTAAAACAGGACCGGAAGGCAAAATCGGAGATGGCAAAATCTTTGTGATGCCTCTTGAAGAAGTGATTCGCATTCGAAG
TGGTGAACGAGGAAACAAAGCGATTTAA

Upstream 100 bases:

>100_bases
TTGAGAGAACAATTGGATTTAGAATTGAAGAAGACAAAGAAATCACGGGCCTCGACCAAGAGATCCATGGTGAAAAAGGA
TACGATATCAGGTAAAAAAT

Downstream 100 bases:

>100_bases
AAGAATCAACTTATCTCCCATAAAATCCATTGGATTCTATGGGAGATATGCTCTACGTTTTTACCAATTCTAAAATATAT
TCCTGATTGGACTTACCACC

Product: nitrogen regulatory protein PII

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 115; Mature: 115

Protein sequence:

>115_residues
MKLVIAIIQPHKLEEVKNELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGPEGKIGDGKIFVMPLEEVIRIRSGERGNKAI

Sequences:

>Translated_115_residues
MKLVIAIIQPHKLEEVKNELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGPEGKIGDGKIFVMPLEEVIRIRSGERGNKAI
>Mature_115_residues
MKLVIAIIQPHKLEEVKNELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKTGPEGKIGDGKIFVMPLEEVIRIRSGERGNKAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=115, Percent_Identity=50.4347826086956, Blast_Score=112, Evalue=6e-27,
Organism=Escherichia coli, GI1786655, Length=115, Percent_Identity=50.4347826086956, Blast_Score=111, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12984; Mature: 12984

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVIAIIQPHKLEEVKNELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVR
CEEEEEEECCCHHHHHHHHHCHHHEEEEEHHHHCCCCCCCCCCCEECCCCHHHHHHHHEE
LEIAVNDEFVKPTVDAILKAAKTGPEGKIGDGKIFVMPLEEVIRIRSGERGNKAI
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLVIAIIQPHKLEEVKNELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYQVNLLRKVR
CEEEEEEECCCHHHHHHHHHCHHHEEEEEHHHHCCCCCCCCCCCEECCCCHHHHHHHHEE
LEIAVNDEFVKPTVDAILKAAKTGPEGKIGDGKIFVMPLEEVIRIRSGERGNKAI
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]