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The map label for this gene is gltX

Identifier: 183220089

GI number: 183220089

Start: 682296

End: 683849

Strand: Reverse

Name: gltX

Synonym: LEPBI_I0677

Alternate gene names: 183220089

Gene position: 683849-682296 (Counterclockwise)

Preceding gene: 183220090

Following gene: 183220088

Centisome position: 19.0

GC content: 42.86

Gene sequence:

>1554_bases
ATGACAGAAGTTCGTACCCGTTTTGCCCCATCCCCCTCCGGATTCCTTCATGTAGGAGGAGCAAGGACTGCCTTATTCAA
TTATTTGTATGCCAAGGCCAAAAAGGGAAAATTTTTACTACGCATCGAAGATACTGACCAAGACAGATCCACGGAAGCAT
CTTTTAAAATCATTTTAGAATCTCTTAAATGGTTGGGTATGGAATGGGACGAAGGTCCTGGTGTGGGTGGACCCAACGGA
CCGTACACGCAATCAGAACGAATTCACATCTACAAAGAATACACTGACAAACTGATCCAAGAAAAAAAAGCATACCGTTG
TTTTTGTACAGCGGAAGAACTCGAAGGCAAAAAAAAACAAGCCGACGCCATGGGAATTCCTTACATCTATGATGGAAAAT
GTTCTGATCTGTCCGATGCAGAAATAGGTTCCCAACTCGAGAAAAAAATTCCCTTTACGGTTCGATTCAAAACACCACAC
AAAATTGTCATCGTAGATGATATGATCCAAGGGAAAGTGAAATTTGAATCCAAACTCATTGGTGATTTTATCATAGTCAA
ATCGGATGGATTTCCTTCATATAACTATGCTGTGGTGATCGATGATGCGTTGATGAAGATCACACACGTGATTCGTGGTG
TGGGCCATCTCTCTAACACTCCTAGACAAATTTTAATCTTTGAAGCATTTGGATTCCCACTCCCAAGATTTGCTCATGCC
AGTGAAATTGTAGGCACTGATGGAAAAAAACTTTCCAAACGTGCCGGTGCCACTTCCGTTCTTGCCTTCCGTGATTTAGG
TTACTCCAGCGAAACCATGCGAAACTATATGGCTCTTCTTGGTTGGACTTCTCCTGACGGCAAAGAATACATGAGTGATG
AAGAGTTGTGTTCTGTTTTTGATGTGGAACGCTGCTCGAAGTCCCCTGCTACCTTTGATGTCTTCAAAAAACTAAAAGAA
GAAGAAAAAGAATCCGTTGATTTTAATAAACTCACGATCCTTGGTCTTGCCGAATACCTAAACCCTAAATCCAAACTCAA
TTGGATGTCGAACAAATACATCCGTGACACAAAAATTGAAACCCTTGGCAAAGCATTAGAACCTTTTTTAAAAGACTGCC
AAATTCCGGAAGCCTTCAAGTCAGGAGAGAACCCACAACTCCTTTCCATCTTAGATTCGGTACGTGTGTATTTGGACCGA
CTCATCCAAGCCCCTCCTTATATTGAGGAATTCTTTTTGGAAAACCTGTCCTTTGAAAACGATGAAGCCAAACAATTGGT
GCTCGAAGGCAAAGGGAAGGAAGTGGTCGCAGAGTTTTACCGCATTGTCAAGGAGAGCTCACTTACCACTCCAGATGCCT
ATAAGGAATCCATGGCAAAAGTGGGCGAAATCACGGGAGAAAAGGGACGAACTCTTTTTATGCCGATCCGAGCCATCACA
ACTGGGAAATCCCATGGATTGGAGCTTCCGATTCTCTTTAGCCTACTCGGTCAGGAAAAGATGGTCAAACGGATGGAACA
ATTGGCGGGCATCTTAGGCATTTCGCTTAGGTAA

Upstream 100 bases:

>100_bases
TATACTTTGTTCCTCCCTATACCAATGGTCTCACAACCAAAACTAAGGAACCATTTGGTTATAAACGAATGGGAGATTTT
TTACTCAAAGAGGAAATGAA

Downstream 100 bases:

>100_bases
ATTTTTTACAGATTCGTTCGGTTTTCGGGTATTTTTGACTTTTTTTCTCACTTCGCATCGTGTAATATGATGAGAGAGAG
GAAAAGGCGGCCATTGACAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTEVRTRFAPSPSGFLHVGGARTALFNYLYAKAKKGKFLLRIEDTDQDRSTEASFKIILESLKWLGMEWDEGPGVGGPNG
PYTQSERIHIYKEYTDKLIQEKKAYRCFCTAEELEGKKKQADAMGIPYIYDGKCSDLSDAEIGSQLEKKIPFTVRFKTPH
KIVIVDDMIQGKVKFESKLIGDFIIVKSDGFPSYNYAVVIDDALMKITHVIRGVGHLSNTPRQILIFEAFGFPLPRFAHA
SEIVGTDGKKLSKRAGATSVLAFRDLGYSSETMRNYMALLGWTSPDGKEYMSDEELCSVFDVERCSKSPATFDVFKKLKE
EEKESVDFNKLTILGLAEYLNPKSKLNWMSNKYIRDTKIETLGKALEPFLKDCQIPEAFKSGENPQLLSILDSVRVYLDR
LIQAPPYIEEFFLENLSFENDEAKQLVLEGKGKEVVAEFYRIVKESSLTTPDAYKESMAKVGEITGEKGRTLFMPIRAIT
TGKSHGLELPILFSLLGQEKMVKRMEQLAGILGISLR

Sequences:

>Translated_517_residues
MTEVRTRFAPSPSGFLHVGGARTALFNYLYAKAKKGKFLLRIEDTDQDRSTEASFKIILESLKWLGMEWDEGPGVGGPNG
PYTQSERIHIYKEYTDKLIQEKKAYRCFCTAEELEGKKKQADAMGIPYIYDGKCSDLSDAEIGSQLEKKIPFTVRFKTPH
KIVIVDDMIQGKVKFESKLIGDFIIVKSDGFPSYNYAVVIDDALMKITHVIRGVGHLSNTPRQILIFEAFGFPLPRFAHA
SEIVGTDGKKLSKRAGATSVLAFRDLGYSSETMRNYMALLGWTSPDGKEYMSDEELCSVFDVERCSKSPATFDVFKKLKE
EEKESVDFNKLTILGLAEYLNPKSKLNWMSNKYIRDTKIETLGKALEPFLKDCQIPEAFKSGENPQLLSILDSVRVYLDR
LIQAPPYIEEFFLENLSFENDEAKQLVLEGKGKEVVAEFYRIVKESSLTTPDAYKESMAKVGEITGEKGRTLFMPIRAIT
TGKSHGLELPILFSLLGQEKMVKRMEQLAGILGISLR
>Mature_516_residues
TEVRTRFAPSPSGFLHVGGARTALFNYLYAKAKKGKFLLRIEDTDQDRSTEASFKIILESLKWLGMEWDEGPGVGGPNGP
YTQSERIHIYKEYTDKLIQEKKAYRCFCTAEELEGKKKQADAMGIPYIYDGKCSDLSDAEIGSQLEKKIPFTVRFKTPHK
IVIVDDMIQGKVKFESKLIGDFIIVKSDGFPSYNYAVVIDDALMKITHVIRGVGHLSNTPRQILIFEAFGFPLPRFAHAS
EIVGTDGKKLSKRAGATSVLAFRDLGYSSETMRNYMALLGWTSPDGKEYMSDEELCSVFDVERCSKSPATFDVFKKLKEE
EKESVDFNKLTILGLAEYLNPKSKLNWMSNKYIRDTKIETLGKALEPFLKDCQIPEAFKSGENPQLLSILDSVRVYLDRL
IQAPPYIEEFFLENLSFENDEAKQLVLEGKGKEVVAEFYRIVKESSLTTPDAYKESMAKVGEITGEKGRTLFMPIRAITT
GKSHGLELPILFSLLGQEKMVKRMEQLAGILGISLR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=37.1601208459215, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=26.953125, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788743, Length=511, Percent_Identity=34.2465753424658, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI226510925, Length=260, Percent_Identity=33.4615384615385, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786895, Length=342, Percent_Identity=22.2222222222222, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=335, Percent_Identity=38.8059701492537, Blast_Score=215, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=323, Percent_Identity=43.0340557275542, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6321192, Length=244, Percent_Identity=27.4590163934426, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=114, Percent_Identity=34.2105263157895, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=317, Percent_Identity=42.9022082018927, Blast_Score=270, Evalue=3e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_LEPBA (B0SCM4)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961764.1
- ProteinModelPortal:   B0SCM4
- SMR:   B0SCM4
- GeneID:   6389675
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0654
- HOGENOM:   HBG628189
- OMA:   MANKIRV
- ProtClustDB:   PRK01406
- BioCyc:   LBIF355278:LBF_0654-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58315; Mature: 58184

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00626 RCC1_2

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVRTRFAPSPSGFLHVGGARTALFNYLYAKAKKGKFLLRIEDTDQDRSTEASFKIILE
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
SLKWLGMEWDEGPGVGGPNGPYTQSERIHIYKEYTDKLIQEKKAYRCFCTAEELEGKKKQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEEEEHHHHCCCHHH
ADAMGIPYIYDGKCSDLSDAEIGSQLEKKIPFTVRFKTPHKIVIVDDMIQGKVKFESKLI
HCCCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCHHHHHEEE
GDFIIVKSDGFPSYNYAVVIDDALMKITHVIRGVGHLSNTPRQILIFEAFGFPLPRFAHA
CCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHH
SEIVGTDGKKLSKRAGATSVLAFRDLGYSSETMRNYMALLGWTSPDGKEYMSDEELCSVF
HHHHCCCCHHHHHHCCCHHEEHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHH
DVERCSKSPATFDVFKKLKEEEKESVDFNKLTILGLAEYLNPKSKLNWMSNKYIRDTKIE
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
TLGKALEPFLKDCQIPEAFKSGENPQLLSILDSVRVYLDRLIQAPPYIEEFFLENLSFEN
HHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DEAKQLVLEGKGKEVVAEFYRIVKESSLTTPDAYKESMAKVGEITGEKGRTLFMPIRAIT
CHHHHHEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
TGKSHGLELPILFSLLGQEKMVKRMEQLAGILGISLR
CCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TEVRTRFAPSPSGFLHVGGARTALFNYLYAKAKKGKFLLRIEDTDQDRSTEASFKIILE
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
SLKWLGMEWDEGPGVGGPNGPYTQSERIHIYKEYTDKLIQEKKAYRCFCTAEELEGKKKQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEEEEHHHHCCCHHH
ADAMGIPYIYDGKCSDLSDAEIGSQLEKKIPFTVRFKTPHKIVIVDDMIQGKVKFESKLI
HCCCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCHHHHHEEE
GDFIIVKSDGFPSYNYAVVIDDALMKITHVIRGVGHLSNTPRQILIFEAFGFPLPRFAHA
CCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHH
SEIVGTDGKKLSKRAGATSVLAFRDLGYSSETMRNYMALLGWTSPDGKEYMSDEELCSVF
HHHHCCCCHHHHHHCCCHHEEHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHH
DVERCSKSPATFDVFKKLKEEEKESVDFNKLTILGLAEYLNPKSKLNWMSNKYIRDTKIE
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
TLGKALEPFLKDCQIPEAFKSGENPQLLSILDSVRVYLDRLIQAPPYIEEFFLENLSFEN
HHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
DEAKQLVLEGKGKEVVAEFYRIVKESSLTTPDAYKESMAKVGEITGEKGRTLFMPIRAIT
CHHHHHEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
TGKSHGLELPILFSLLGQEKMVKRMEQLAGILGISLR
CCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA