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The map label for this gene is ilvI [H]

Identifier: 183220021

GI number: 183220021

Start: 606806

End: 608569

Strand: Reverse

Name: ilvI [H]

Synonym: LEPBI_I0605

Alternate gene names: 183220021

Gene position: 608569-606806 (Counterclockwise)

Preceding gene: 183220022

Following gene: 183220020

Centisome position: 16.91

GC content: 39.51

Gene sequence:

>1764_bases
ATGAAAAAAACTGGCGCATGGTTATTACGTTTTGCACTCGAAGAAATTGGAGTGCGTTATACATTCGGAATCCCTGGAGT
TCACAATACAGAAATTTATGATGAACTCAACAATTCCAATTCAATCCAACCAATACTTGTTACACATGAAGGATGTGGTG
CCTTTATGGCAGATGCGATCAGCAGAACGAGTCATTCGATAGGAACACTGGTCATTGTGCCTGCAGCGGGAGTCACTCAT
GCTGCAAGTGGAATTGGGGAAGCCTATTTGGATGGCATTCCTATGTTAGTGATATCAGGAGGTGTGCGAAGTGATTCCAA
ATTTAAATACCAATTACATGACATGGACCAACACTCGTTATTAAAACCGATCACGAAACAAACCTTCAAGATCAAATCAC
ATTCGGAAATCATCGAAACGATTTACCAAGCATATCAAATTGCAACTTCAGGAGAACCAGGACCCGTTTTTGTGGAAATC
CCAGTCAATATTCAATTGTATGCCGAAAATGTGCCGTCAATCCAATCCTATCATACGTATTGCAACAAACAAACAATTGA
TCCAAGTCCACCGCAGGTACAATCCATTGCCGAAGCCGTGGAACTTCTTTTGGAAGCGAAGGCTCCCGGTATTTTTTTAG
GTTGGGGAGCCGTTGATACAACAAAAGAGAGTATCGAATTGGCAGAATTGTTAGTTGCTCCGGTTGCTACAACCTTACAG
GGATTAAGTTCCTTTCCGGGCAACCATCCATTATTTTGTGGGATGAGTTTTGGCGAAGCAGCTGTTCCTGCTGCCACAAA
TGCGTTTCTTCACTGTGATTGTTTGCTCGCCATCGGCACAAGGTTTTCTGAAATTGCAACGGCAAGTTTTGGTACCAAGG
TTCCAAAAAATTTAATTCACATTGATATCAATCCGGATGTATTCAATCAAAACTATCCAAGTAAAATTTCGATTGAAGGT
GATTCAAAAATCATTCTACCAATCCTACTACAAAAACTAAAAGAAAGATTGGTATTCAGAACAGGAGATACAAACTCCAG
AATGGTATCATTAACAGAGACAATCAAAATAAACAAACAGCAATACATTGATGAATGGCTGTTACATGATAGTAAAAACA
AGGTAAACCCTTGTCAGTTTTTTTTAAACCTGAGAAACCGATTGCCTGATGATGGATTTGTTGTTGTCGATGATGGAAAC
CATACATTTTTAACCGCCGAACTGATGCCCATCCATAAACCTCGTCACTTTATCTCACCAACTGATTTTAACTGTATGGG
TTATGCAGTACCCGCTACCATTGCAACCAAACTTGCAAACCCAGATAAAGATGTCATAGGAATCATTGGTGATGGAGCTT
TTTTAATGACTTGTATGGAAATAGTGACAGCATCAAAAAATAAAATTGGTGTTGTGTTTGTTGTCTTCAATGATGGAGAG
TTAGCACAAATTGCCCAAGCACAAGAAATTCCTTACAATCGAAAAACCTGCACCATCCTTGGGACAACAGAATTTGAACA
CATTGCTTTGGCAACAGGTGCAGCATACATTCGTTTAGAGACCAATGATAAGATAAAAGAGTCTCTTCAAATCGCATTCA
GCTTGGCAAAAGAAGGGAAACCTGTTATTTTGGATGTGAGTATCGATTATAGCAAAAAAACCAGATTTACGAAGGGCATT
GTTGGAACCAATCTACAACGATTACCATTTGCTACAAAAGTGAGAATGATTGGACGTGCGATCATCAGGAAATTGACAGG
ATAA

Upstream 100 bases:

>100_bases
AAGGAACATTTTTATCAGGGTGTATCCTTACGGCACGAAGTGCAGCAAAATCAATCTGTAGTTTAAAATAGATGTTCGTG
ATATTTAAGGAGATACTGTT

Downstream 100 bases:

>100_bases
AAAACATTTGAGAATCATACTGGCTCCAATGGAAGGACTTTTGGACTTTCGATTGCGAGAAATTTTAACACAGATTGGTG
GATACGATGAATGTGTTAGC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKKTGAWLLRFALEEIGVRYTFGIPGVHNTEIYDELNNSNSIQPILVTHEGCGAFMADAISRTSHSIGTLVIVPAAGVTH
AASGIGEAYLDGIPMLVISGGVRSDSKFKYQLHDMDQHSLLKPITKQTFKIKSHSEIIETIYQAYQIATSGEPGPVFVEI
PVNIQLYAENVPSIQSYHTYCNKQTIDPSPPQVQSIAEAVELLLEAKAPGIFLGWGAVDTTKESIELAELLVAPVATTLQ
GLSSFPGNHPLFCGMSFGEAAVPAATNAFLHCDCLLAIGTRFSEIATASFGTKVPKNLIHIDINPDVFNQNYPSKISIEG
DSKIILPILLQKLKERLVFRTGDTNSRMVSLTETIKINKQQYIDEWLLHDSKNKVNPCQFFLNLRNRLPDDGFVVVDDGN
HTFLTAELMPIHKPRHFISPTDFNCMGYAVPATIATKLANPDKDVIGIIGDGAFLMTCMEIVTASKNKIGVVFVVFNDGE
LAQIAQAQEIPYNRKTCTILGTTEFEHIALATGAAYIRLETNDKIKESLQIAFSLAKEGKPVILDVSIDYSKKTRFTKGI
VGTNLQRLPFATKVRMIGRAIIRKLTG

Sequences:

>Translated_587_residues
MKKTGAWLLRFALEEIGVRYTFGIPGVHNTEIYDELNNSNSIQPILVTHEGCGAFMADAISRTSHSIGTLVIVPAAGVTH
AASGIGEAYLDGIPMLVISGGVRSDSKFKYQLHDMDQHSLLKPITKQTFKIKSHSEIIETIYQAYQIATSGEPGPVFVEI
PVNIQLYAENVPSIQSYHTYCNKQTIDPSPPQVQSIAEAVELLLEAKAPGIFLGWGAVDTTKESIELAELLVAPVATTLQ
GLSSFPGNHPLFCGMSFGEAAVPAATNAFLHCDCLLAIGTRFSEIATASFGTKVPKNLIHIDINPDVFNQNYPSKISIEG
DSKIILPILLQKLKERLVFRTGDTNSRMVSLTETIKINKQQYIDEWLLHDSKNKVNPCQFFLNLRNRLPDDGFVVVDDGN
HTFLTAELMPIHKPRHFISPTDFNCMGYAVPATIATKLANPDKDVIGIIGDGAFLMTCMEIVTASKNKIGVVFVVFNDGE
LAQIAQAQEIPYNRKTCTILGTTEFEHIALATGAAYIRLETNDKIKESLQIAFSLAKEGKPVILDVSIDYSKKTRFTKGI
VGTNLQRLPFATKVRMIGRAIIRKLTG
>Mature_587_residues
MKKTGAWLLRFALEEIGVRYTFGIPGVHNTEIYDELNNSNSIQPILVTHEGCGAFMADAISRTSHSIGTLVIVPAAGVTH
AASGIGEAYLDGIPMLVISGGVRSDSKFKYQLHDMDQHSLLKPITKQTFKIKSHSEIIETIYQAYQIATSGEPGPVFVEI
PVNIQLYAENVPSIQSYHTYCNKQTIDPSPPQVQSIAEAVELLLEAKAPGIFLGWGAVDTTKESIELAELLVAPVATTLQ
GLSSFPGNHPLFCGMSFGEAAVPAATNAFLHCDCLLAIGTRFSEIATASFGTKVPKNLIHIDINPDVFNQNYPSKISIEG
DSKIILPILLQKLKERLVFRTGDTNSRMVSLTETIKINKQQYIDEWLLHDSKNKVNPCQFFLNLRNRLPDDGFVVVDDGN
HTFLTAELMPIHKPRHFISPTDFNCMGYAVPATIATKLANPDKDVIGIIGDGAFLMTCMEIVTASKNKIGVVFVVFNDGE
LAQIAQAQEIPYNRKTCTILGTTEFEHIALATGAAYIRLETNDKIKESLQIAFSLAKEGKPVILDVSIDYSKKTRFTKGI
VGTNLQRLPFATKVRMIGRAIIRKLTG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=584, Percent_Identity=26.027397260274, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.3816254416961, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI87081685, Length=561, Percent_Identity=27.9857397504456, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=26.5822784810127, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=24.6017699115044, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=23.3576642335766, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1788716, Length=574, Percent_Identity=23.5191637630662, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=595, Percent_Identity=24.8739495798319, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=23.8178633975482, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=23.8178633975482, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=26.2786596119929, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=26.6528925619835, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320588, Length=525, Percent_Identity=22.2857142857143, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=25.1546391752577, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64364; Mature: 64364

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTGAWLLRFALEEIGVRYTFGIPGVHNTEIYDELNNSNSIQPILVTHEGCGAFMADAI
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHH
SRTSHSIGTLVIVPAAGVTHAASGIGEAYLDGIPMLVISGGVRSDSKFKYQLHDMDQHSL
HHCCCCCCEEEEEECCCCHHHHHCCCHHHHCCCCEEEEECCCCCCCEEEEEEECCCHHHH
LKPITKQTFKIKSHSEIIETIYQAYQIATSGEPGPVFVEIPVNIQLYAENVPSIQSYHTY
HHHHHHHHEEECCHHHHHHHHHHHHEEECCCCCCCEEEEECCEEEEEECCCCCHHHHHHH
CNKQTIDPSPPQVQSIAEAVELLLEAKAPGIFLGWGAVDTTKESIELAELLVAPVATTLQ
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GLSSFPGNHPLFCGMSFGEAAVPAATNAFLHCDCLLAIGTRFSEIATASFGTKVPKNLIH
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHCCCCCCCCEEE
IDINPDVFNQNYPSKISIEGDSKIILPILLQKLKERLVFRTGDTNSRMVSLTETIKINKQ
EECCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHEEECCCCCCEEEEEEHHEEECHH
QYIDEWLLHDSKNKVNPCQFFLNLRNRLPDDGFVVVDDGNHTFLTAELMPIHKPRHFISP
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEEEECCCCCCCCCC
TDFNCMGYAVPATIATKLANPDKDVIGIIGDGAFLMTCMEIVTASKNKIGVVFVVFNDGE
CCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
LAQIAQAQEIPYNRKTCTILGTTEFEHIALATGAAYIRLETNDKIKESLQIAFSLAKEGK
HHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCC
PVILDVSIDYSKKTRFTKGIVGTNLQRLPFATKVRMIGRAIIRKLTG
CEEEEEEECCCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTGAWLLRFALEEIGVRYTFGIPGVHNTEIYDELNNSNSIQPILVTHEGCGAFMADAI
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHH
SRTSHSIGTLVIVPAAGVTHAASGIGEAYLDGIPMLVISGGVRSDSKFKYQLHDMDQHSL
HHCCCCCCEEEEEECCCCHHHHHCCCHHHHCCCCEEEEECCCCCCCEEEEEEECCCHHHH
LKPITKQTFKIKSHSEIIETIYQAYQIATSGEPGPVFVEIPVNIQLYAENVPSIQSYHTY
HHHHHHHHEEECCHHHHHHHHHHHHEEECCCCCCCEEEEECCEEEEEECCCCCHHHHHHH
CNKQTIDPSPPQVQSIAEAVELLLEAKAPGIFLGWGAVDTTKESIELAELLVAPVATTLQ
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GLSSFPGNHPLFCGMSFGEAAVPAATNAFLHCDCLLAIGTRFSEIATASFGTKVPKNLIH
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHCCCCCCCCEEE
IDINPDVFNQNYPSKISIEGDSKIILPILLQKLKERLVFRTGDTNSRMVSLTETIKINKQ
EECCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHEEECCCCCCEEEEEEHHEEECHH
QYIDEWLLHDSKNKVNPCQFFLNLRNRLPDDGFVVVDDGNHTFLTAELMPIHKPRHFISP
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEEEECCCCCCCCCC
TDFNCMGYAVPATIATKLANPDKDVIGIIGDGAFLMTCMEIVTASKNKIGVVFVVFNDGE
CCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCC
LAQIAQAQEIPYNRKTCTILGTTEFEHIALATGAAYIRLETNDKIKESLQIAFSLAKEGK
HHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCC
PVILDVSIDYSKKTRFTKGIVGTNLQRLPFATKVRMIGRAIIRKLTG
CEEEEEEECCCCCCHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]