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The map label for this gene is dusC [H]

Identifier: 183220020

GI number: 183220020

Start: 605848

End: 606798

Strand: Reverse

Name: dusC [H]

Synonym: LEPBI_I0604

Alternate gene names: 183220020

Gene position: 606798-605848 (Counterclockwise)

Preceding gene: 183220021

Following gene: 183220019

Centisome position: 16.86

GC content: 40.8

Gene sequence:

>951_bases
TTGAGAATCATACTGGCTCCAATGGAAGGACTTTTGGACTTTCGATTGCGAGAAATTTTAACACAGATTGGTGGATACGA
TGAATGTGTTAGCGAATTCATTCGTGTGAATGATACACTCCTCCCTCCACATCGATTTTTTCGAACGGCACCTGAGCTCA
AAAACTCATCCCGAACAAAATCCGATACTCCTGTTAAAATTCAGATCCTTGGTTCCGATCCAAATCGAATGGCAGATAAC
GCACATTTGGTGGCATCCCTTGGAGCATATGGAATCGACATAAACTTTGGGTGCCCTGCCCCTACGGTCAATCGGAATCG
TGGCGGTGCAGCCCTTTTGAAAGAACCGAACCTTATGTATTCAATCGTAAAAGCCGTTCGAAATGTTGTGCCAGAGAATA
TTCCAGTGACGGCAAAAATGCGACTTGGTTACGAATCCACCGAACTGGCGCTAGACTGTGCTAAGGCATTAGAAGAAGGT
GGTGCCAAAGAAATTGTGGTCCATGCAAGGACAAAAACAGATGGCTACAAACCTCCTGCCTATTGGGAGTGGATTGCCAA
AATCAAATCCATTTTAAAAATCAATGTCATTGCGAATGGAGAAATCTGGACAGTGCAAGATGCAATCCAATGCCAAAAGA
TTTCTGGTTGCGATGACATTATGATTGGGCGGGGTGCTGTGACAAATCCATCCATTGCCCTTCTCATTAAAAAAAAACTA
GAGGAACGAATGAGTTGGCAGAAAACCAAAGAAGTTCTCTCGTCTTATTGGTTATCAATGGAACCTGATGTGGAAGCTGT
TAGTAGAGTTGGAAGAATAAAACAATGGTTACATTATCTTTCCAGAGAATATGAAGAAGCTCAAATTGACTTTCAAAAGA
TAAAAAGAATTTCAAATCCAAAAGATTTCGAATTAATTTACTTTTCAGAATTATTTTCTCAGATAAAGTGA

Upstream 100 bases:

>100_bases
AAGGGCATTGTTGGAACCAATCTACAACGATTACCATTTGCTACAAAAGTGAGAATGATTGGACGTGCGATCATCAGGAA
ATTGACAGGATAAAAAACAT

Downstream 100 bases:

>100_bases
AAGAATCAATTCTCCTTATGAGGAAACTTTATGTTTGAATCATTTACAAATTCTGAAATTTTATCTGGAATGATCACACC
AGCAGTTTTGGTTTCTGCCT

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRIILAPMEGLLDFRLREILTQIGGYDECVSEFIRVNDTLLPPHRFFRTAPELKNSSRTKSDTPVKIQILGSDPNRMADN
AHLVASLGAYGIDINFGCPAPTVNRNRGGAALLKEPNLMYSIVKAVRNVVPENIPVTAKMRLGYESTELALDCAKALEEG
GAKEIVVHARTKTDGYKPPAYWEWIAKIKSILKINVIANGEIWTVQDAIQCQKISGCDDIMIGRGAVTNPSIALLIKKKL
EERMSWQKTKEVLSSYWLSMEPDVEAVSRVGRIKQWLHYLSREYEEAQIDFQKIKRISNPKDFELIYFSELFSQIK

Sequences:

>Translated_316_residues
MRIILAPMEGLLDFRLREILTQIGGYDECVSEFIRVNDTLLPPHRFFRTAPELKNSSRTKSDTPVKIQILGSDPNRMADN
AHLVASLGAYGIDINFGCPAPTVNRNRGGAALLKEPNLMYSIVKAVRNVVPENIPVTAKMRLGYESTELALDCAKALEEG
GAKEIVVHARTKTDGYKPPAYWEWIAKIKSILKINVIANGEIWTVQDAIQCQKISGCDDIMIGRGAVTNPSIALLIKKKL
EERMSWQKTKEVLSSYWLSMEPDVEAVSRVGRIKQWLHYLSREYEEAQIDFQKIKRISNPKDFELIYFSELFSQIK
>Mature_316_residues
MRIILAPMEGLLDFRLREILTQIGGYDECVSEFIRVNDTLLPPHRFFRTAPELKNSSRTKSDTPVKIQILGSDPNRMADN
AHLVASLGAYGIDINFGCPAPTVNRNRGGAALLKEPNLMYSIVKAVRNVVPENIPVTAKMRLGYESTELALDCAKALEEG
GAKEIVVHARTKTDGYKPPAYWEWIAKIKSILKINVIANGEIWTVQDAIQCQKISGCDDIMIGRGAVTNPSIALLIKKKL
EERMSWQKTKEVLSSYWLSMEPDVEAVSRVGRIKQWLHYLSREYEEAQIDFQKIKRISNPKDFELIYFSELFSQIK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=177, Percent_Identity=32.7683615819209, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI239788483, Length=291, Percent_Identity=25.4295532646048, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI239788462, Length=226, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI8923374, Length=214, Percent_Identity=32.7102803738318, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788462, Length=304, Percent_Identity=47.0394736842105, Blast_Score=287, Evalue=6e-79,
Organism=Escherichia coli, GI1789660, Length=255, Percent_Identity=34.1176470588235, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693211, Length=266, Percent_Identity=25.9398496240602, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=177, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=274, Percent_Identity=26.2773722627737, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=281, Percent_Identity=23.8434163701068, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=187, Percent_Identity=28.3422459893048, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=185, Percent_Identity=28.6486486486486, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=285, Percent_Identity=28.0701754385965, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920448, Length=285, Percent_Identity=28.0701754385965, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921524, Length=183, Percent_Identity=30.0546448087432, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585320, Length=298, Percent_Identity=26.510067114094, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=145, Percent_Identity=32.4137931034483, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35743; Mature: 35743

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIILAPMEGLLDFRLREILTQIGGYDECVSEFIRVNDTLLPPHRFFRTAPELKNSSRTK
CEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCC
SDTPVKIQILGSDPNRMADNAHLVASLGAYGIDINFGCPAPTVNRNRGGAALLKEPNLMY
CCCCEEEEEECCCCCCCCCCHHHHEECCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHH
SIVKAVRNVVPENIPVTAKMRLGYESTELALDCAKALEEGGAKEIVVHARTKTDGYKPPA
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
YWEWIAKIKSILKINVIANGEIWTVQDAIQCQKISGCDDIMIGRGAVTNPSIALLIKKKL
HHHHHHHHHHHHEEEEEECCCEEEEHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHH
EERMSWQKTKEVLSSYWLSMEPDVEAVSRVGRIKQWLHYLSREYEEAQIDFQKIKRISNP
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KDFELIYFSELFSQIK
CCEEEEEHHHHHHHCC
>Mature Secondary Structure
MRIILAPMEGLLDFRLREILTQIGGYDECVSEFIRVNDTLLPPHRFFRTAPELKNSSRTK
CEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCC
SDTPVKIQILGSDPNRMADNAHLVASLGAYGIDINFGCPAPTVNRNRGGAALLKEPNLMY
CCCCEEEEEECCCCCCCCCCHHHHEECCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHH
SIVKAVRNVVPENIPVTAKMRLGYESTELALDCAKALEEGGAKEIVVHARTKTDGYKPPA
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCH
YWEWIAKIKSILKINVIANGEIWTVQDAIQCQKISGCDDIMIGRGAVTNPSIALLIKKKL
HHHHHHHHHHHHEEEEEECCCEEEEHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHH
EERMSWQKTKEVLSSYWLSMEPDVEAVSRVGRIKQWLHYLSREYEEAQIDFQKIKRISNP
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KDFELIYFSELFSQIK
CCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]