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The map label for this gene is truA

Identifier: 183219967

GI number: 183219967

Start: 547619

End: 548413

Strand: Reverse

Name: truA

Synonym: LEPBI_I0549

Alternate gene names: 183219967

Gene position: 548413-547619 (Counterclockwise)

Preceding gene: 183219968

Following gene: 183219966

Centisome position: 15.24

GC content: 44.53

Gene sequence:

>795_bases
TTGCCCAATTACGCACTTCTTGTCGAATATGATGGCACTCATTTTAATGGGTGGCAAAAACAAAAGAACCTTCCGACTGT
CCAATCTTCGATCGAATCGGCACTTGGCATCATCCTCAGAAGAAATCCCGCCTCACGTCTGTCAGTGGCTGGAAGGACCG
ATACAGGTGTTCATGCCCTCGGAATGGTATGTAATTTCAAAACGGAACATCCCATTCCCAATTTTCATAAACTACTCGTT
TCACTGAATGCGCTCACTCCGACTGGTGTTTCTGTCAAAAATGTAGTCGAAGTACCTTCTGATTTCCATGCGCGCTTCAG
TTGTACGGGTAGGGAATACATTTATAAATTGTACTATAGCAAATATGAAAGTAGTTTTGTGGAAGGACGTGCTTTTTGGG
TCAAAGGCCATATTGACTGGGAAAGAGTCAAAAAACAACTCCAGGTCCTAGTCGGCGAAAAGGATTTTCGTTCCTTCACA
AAAGCAAAGTCAATGGCAGGGAAACGAGCGGTCAGAGAAATTTTAGCCATCCAGTTAGAGAATTTGTCCCCCGAATGGTA
CCAGATTCGGATCCGAGCCAATGGATTTATGCACAATATGGTGCGAATCACAGTAGGAACTTTACTGGACATAGGGAAGG
GACGTTGGGAATCTAGATCCATAGACTCCATTTTGGAAGAAAAGAACCGTACGCAGGCGGGAGTCACTCTCCCACCTGAT
GGGCTCTATTTTGTCCGCGCGTATTACGAAGATCATCCGGAAATTCATGAATTGTACAAAATCCCTCTTCCTTAG

Upstream 100 bases:

>100_bases
GCAAGTAAAGGGAAACCAGGCCCTATTGTGGAAATGACACGTGAGTTATACGACGAATACAATGCCATTTTAGAAGCAAT
GGGAGGCGAGAAGTAAGGGT

Downstream 100 bases:

>100_bases
CATCCTTCTCATTTTCACCATTTTTTCTGGCTCGCTCGCTTCGCAAACCTTGGAGGAGTACAACAAACGTCGCTATGAAA
TTGGAGGTTGGGTCGGTGCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPNYALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLSVAGRTDTGVHALGMVCNFKTEHPIPNFHKLLV
SLNALTPTGVSVKNVVEVPSDFHARFSCTGREYIYKLYYSKYESSFVEGRAFWVKGHIDWERVKKQLQVLVGEKDFRSFT
KAKSMAGKRAVREILAIQLENLSPEWYQIRIRANGFMHNMVRITVGTLLDIGKGRWESRSIDSILEEKNRTQAGVTLPPD
GLYFVRAYYEDHPEIHELYKIPLP

Sequences:

>Translated_264_residues
MPNYALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLSVAGRTDTGVHALGMVCNFKTEHPIPNFHKLLV
SLNALTPTGVSVKNVVEVPSDFHARFSCTGREYIYKLYYSKYESSFVEGRAFWVKGHIDWERVKKQLQVLVGEKDFRSFT
KAKSMAGKRAVREILAIQLENLSPEWYQIRIRANGFMHNMVRITVGTLLDIGKGRWESRSIDSILEEKNRTQAGVTLPPD
GLYFVRAYYEDHPEIHELYKIPLP
>Mature_263_residues
PNYALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLSVAGRTDTGVHALGMVCNFKTEHPIPNFHKLLVS
LNALTPTGVSVKNVVEVPSDFHARFSCTGREYIYKLYYSKYESSFVEGRAFWVKGHIDWERVKKQLQVLVGEKDFRSFTK
AKSMAGKRAVREILAIQLENLSPEWYQIRIRANGFMHNMVRITVGTLLDIGKGRWESRSIDSILEEKNRTQAGVTLPPDG
LYFVRAYYEDHPEIHELYKIPLP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=26.2773722627737, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788657, Length=259, Percent_Identity=34.7490347490347, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321112, Length=240, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007752, Length=309, Percent_Identity=26.2135922330097, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648455, Length=240, Percent_Identity=24.1666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362146, Length=240, Percent_Identity=24.1666666666667, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_LEPBA (B0SC03)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961646.1
- GeneID:   6388541
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0530
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   CLSK2336343
- BioCyc:   LBIF355278:LBF_0530-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30213; Mature: 30082

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNYALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLSVAGRTDTGVHAL
CCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHEEEECCCHHHEEECCCCCCCHHHH
GMVCNFKTEHPIPNFHKLLVSLNALTPTGVSVKNVVEVPSDFHARFSCTGREYIYKLYYS
HHEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHH
KYESSFVEGRAFWVKGHIDWERVKKQLQVLVGEKDFRSFTKAKSMAGKRAVREILAIQLE
HHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLSPEWYQIRIRANGFMHNMVRITVGTLLDIGKGRWESRSIDSILEEKNRTQAGVTLPPD
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
GLYFVRAYYEDHPEIHELYKIPLP
CEEEEEEECCCCCCHHHHEECCCC
>Mature Secondary Structure 
PNYALLVEYDGTHFNGWQKQKNLPTVQSSIESALGIILRRNPASRLSVAGRTDTGVHAL
CCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHEEEECCCHHHEEECCCCCCCHHHH
GMVCNFKTEHPIPNFHKLLVSLNALTPTGVSVKNVVEVPSDFHARFSCTGREYIYKLYYS
HHEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHH
KYESSFVEGRAFWVKGHIDWERVKKQLQVLVGEKDFRSFTKAKSMAGKRAVREILAIQLE
HHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLSPEWYQIRIRANGFMHNMVRITVGTLLDIGKGRWESRSIDSILEEKNRTQAGVTLPPD
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
GLYFVRAYYEDHPEIHELYKIPLP
CEEEEEEECCCCCCHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA