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The map label for this gene is gacA [H]

Identifier: 183219607

GI number: 183219607

Start: 176838

End: 177509

Strand: Direct

Name: gacA [H]

Synonym: LEPBI_I0182

Alternate gene names: 183219607

Gene position: 176838-177509 (Clockwise)

Preceding gene: 183219606

Following gene: 183219613

Centisome position: 4.91

GC content: 38.84

Gene sequence:

>672_bases
ATGAAAAAACAAGTCTATATCGTTGATGACCATCCTCTTGTAGTAGATGCACTACAAAACCTAATTGCTAAATCGGAAGA
TTTAGAGTGTATTGGAAGTGCAGATAATATTGAAAAAGCATTTAACGATATAGAACAAATGCAACCGACATTGGTTCTGA
TTGATATCCAACTCAAACAAAACCAAAATGGTTTGCAACTTTTGAAACGTCTGAGGACAACATTTCCAAATATTGCCGTC
ATCATTATCAGTATGTTGACGGATGATACTTTTGTGGATCGTGCATTTAAATTAGGTGCGATGGGTTATGTTTTCAAAGA
AGACACAACCACACAAATTGTAGAAGCAATCCATACCGTTCTAAAAGGTGATTATTTCGTGAGTTCATCCCAAGCGACAA
GGCTCCTTGGGCATCTGTATCGTGCATCCCAGAAAGACGAAAAGGATCCAATCGATAGATTGTCCAATCGTGAATTGGAA
GTGTTTCTCATGATTGGAGAAGGTATGCCGGTGAAAGAAATCGCAGCCAATATGGGCCTTGCCCCATCTACAATTGAAAC
CCTACGATCTCGAATCAAATCGAAACTCAGCATCACTGAAAACGAAAAATTGATTCGTGTAGCGGTTGAGTGGAAGTATA
CCCAAGCCAAAACTGATATTGTCGTTTCTTAA

Upstream 100 bases:

>100_bases
CATTATTCATTGCTGACAACTGTTTGATGTTGTTTGGTGATGGTAAAAAGGCAACGCAAGAAATGATCGCAGCTTTAAAA
GAATCTTAGAGTCGGAAGTT

Downstream 100 bases:

>100_bases
AAATAAATCCAATTCGTTTTAGAATATAATTTTGATCGATTAACTTCTGAATGGAATTGGAATTGATTTCTGAACAAATC
TTCAATCCGAATCTGATTCA

Product: LuxR family response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKKQVYIVDDHPLVVDALQNLIAKSEDLECIGSADNIEKAFNDIEQMQPTLVLIDIQLKQNQNGLQLLKRLRTTFPNIAV
IIISMLTDDTFVDRAFKLGAMGYVFKEDTTTQIVEAIHTVLKGDYFVSSSQATRLLGHLYRASQKDEKDPIDRLSNRELE
VFLMIGEGMPVKEIAANMGLAPSTIETLRSRIKSKLSITENEKLIRVAVEWKYTQAKTDIVVS

Sequences:

>Translated_223_residues
MKKQVYIVDDHPLVVDALQNLIAKSEDLECIGSADNIEKAFNDIEQMQPTLVLIDIQLKQNQNGLQLLKRLRTTFPNIAV
IIISMLTDDTFVDRAFKLGAMGYVFKEDTTTQIVEAIHTVLKGDYFVSSSQATRLLGHLYRASQKDEKDPIDRLSNRELE
VFLMIGEGMPVKEIAANMGLAPSTIETLRSRIKSKLSITENEKLIRVAVEWKYTQAKTDIVVS
>Mature_223_residues
MKKQVYIVDDHPLVVDALQNLIAKSEDLECIGSADNIEKAFNDIEQMQPTLVLIDIQLKQNQNGLQLLKRLRTTFPNIAV
IIISMLTDDTFVDRAFKLGAMGYVFKEDTTTQIVEAIHTVLKGDYFVSSSQATRLLGHLYRASQKDEKDPIDRLSNRELE
VFLMIGEGMPVKEIAANMGLAPSTIETLRSRIKSKLSITENEKLIRVAVEWKYTQAKTDIVVS

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=26.6355140186916, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=28.2178217821782, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788712, Length=190, Percent_Identity=31.0526315789474, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=27.4725274725275, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=24.8756218905473, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=25.3658536585366, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788521, Length=214, Percent_Identity=26.6355140186916, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25155; Mature: 25155

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQVYIVDDHPLVVDALQNLIAKSEDLECIGSADNIEKAFNDIEQMQPTLVLIDIQLKQ
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECC
NQNGLQLLKRLRTTFPNIAVIIISMLTDDTFVDRAFKLGAMGYVFKEDTTTQIVEAIHTV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
LKGDYFVSSSQATRLLGHLYRASQKDEKDPIDRLSNRELEVFLMIGEGMPVKEIAANMGL
HCCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHCCC
APSTIETLRSRIKSKLSITENEKLIRVAVEWKYTQAKTDIVVS
CHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCEEEC
>Mature Secondary Structure
MKKQVYIVDDHPLVVDALQNLIAKSEDLECIGSADNIEKAFNDIEQMQPTLVLIDIQLKQ
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECC
NQNGLQLLKRLRTTFPNIAVIIISMLTDDTFVDRAFKLGAMGYVFKEDTTTQIVEAIHTV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
LKGDYFVSSSQATRLLGHLYRASQKDEKDPIDRLSNRELEVFLMIGEGMPVKEIAANMGL
HCCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHCCC
APSTIETLRSRIKSKLSITENEKLIRVAVEWKYTQAKTDIVVS
CHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]