Click here to switch to the map view.

The map label for this gene is vhcA [H]

Identifier: 182677503

GI number: 182677503

Start: 562375

End: 563664

Strand: Direct

Name: vhcA [H]

Synonym: Bind_0507

Alternate gene names: 182677503

Gene position: 562375-563664 (Clockwise)

Preceding gene: 182677502

Following gene: 182677504

Centisome position: 13.49

GC content: 60.0

Gene sequence:

>1290_bases
ATGGCGACCAGGACGATCCTGGTTGACTATCTGGCTCGCGTCGAGGGCGAAGGAGCGCTCGATATCGAGGTCCATGATGG
CCGCGTCACCTCGGCGCGGCTCAGGATCTTTGAACCGCCGCGTTTCTTCGAGGCGTTCTTGCGCGGCCGGGGCTATGCGG
AGACGCCAGACCTCGTGGCGCGTATCTGCGGCATCTGCCCGATCGCCTATCAGATGAGCGCCGTCCATGCGATTGAAAAC
GCGTTCGGTGTCACGGTCAGTGGCCAACTCCGCGCGCTCAGGCGGCTCATCTATTGCGGCGAATGGATCGAAAGCCATGC
TCTCCATATCGTCATGCTGCATGCACCTGACTTTCTTGGCTTTCCCGATGGGATTCGGATGGCGAGTGAACAGGGTGATG
CCGTTCGCAATGGCTTGGCGCTCAAGAAGACCGGCAACGCGCTGATGCGCCTGATGGGTGGGCGCGAGATTCATCCTGTT
AATGTCAAGACCGGCGGTTTTTATCGCGTGCCCAGCCGGGCAGAACTCGAACCCATTGGGGAGAGCCTTGAACGGGCCCG
CGCTCTGGCCGTCGGCCTTGTCCGCTGGGTCTCCTCCTTTCCCTTTCCCGATTATGAGCGGGACTATGAATTCGTCGCGC
TGCGCCATCCAGACGAATATCCGTTCAATGAGGGGCGACTCGTCTCCAATCGCGGTATCGATATCGATATCGCCGATTAT
GAGACTGAGTTTGAAGAACGTCAGGTGGCCCATTCGACTGCTCTGCATGCCCTGACACGGCGGCGTGGCGCTTATCTTGT
TGGCCCGCTCGCCCGCTATGCGCTGAATTTCGACCGGCTACCCGCTTCTGTTCAGGCACTTGCCCGAGGGGCAGGGCTTC
CCCCAGTCTGCAAGAATCCTTTTCAGAGCATTATCGTGCGGGCACTCGAAATCGTTTATGCCTGCGAGGAAGCGCTGAAG
TTGATCGCGGCCTACGAACCGCCAGAATGCGCGGCGGTGACGGTTATGCCTCGCGCCGGGACGGGCTTTGGCTGCACGGA
GGCACCGCGCGGCCTATTGTGGCACCGCTATGAGTTCGCGCCCGATGGCACGATTGAGCGGGCGCGCATTGTGCCGCCGA
CATCGCAGAATCAGCCGAGTATCGAAGCTGATCTTGCCGCTGTTGCCAGTATGATCCTTGACCAACCTGACGATGTCATC
CGTGCTCGTTGCGAGCAGGCGATCCGCAACTATGATCCCTGCATTTCGTGCTCTGCTCATTTCCTCAAGCTCTCGGTGCG
GCGGACATGA

Upstream 100 bases:

>100_bases
CGCGATATAAGCCGAGCTTTCCGCACCTTCTACGCCGCCGCCGAGCCCTTTCGGAAAGAGAGCGTCGTCCATGCCGACAT
CACCCACGACGACATCACTA

Downstream 100 bases:

>100_bases
CCGGGGCTGGAGAAAAGGAGAGGGGGCTGTACAAGATTCTCGTCGTCGGTCTCGGCAATCCAGACAGGGGCGACGATGGC
ATTGGCGCGGCCGTCATCCA

Product: nickel-dependent hydrogenase large subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MATRTILVDYLARVEGEGALDIEVHDGRVTSARLRIFEPPRFFEAFLRGRGYAETPDLVARICGICPIAYQMSAVHAIEN
AFGVTVSGQLRALRRLIYCGEWIESHALHIVMLHAPDFLGFPDGIRMASEQGDAVRNGLALKKTGNALMRLMGGREIHPV
NVKTGGFYRVPSRAELEPIGESLERARALAVGLVRWVSSFPFPDYERDYEFVALRHPDEYPFNEGRLVSNRGIDIDIADY
ETEFEERQVAHSTALHALTRRRGAYLVGPLARYALNFDRLPASVQALARGAGLPPVCKNPFQSIIVRALEIVYACEEALK
LIAAYEPPECAAVTVMPRAGTGFGCTEAPRGLLWHRYEFAPDGTIERARIVPPTSQNQPSIEADLAAVASMILDQPDDVI
RARCEQAIRNYDPCISCSAHFLKLSVRRT

Sequences:

>Translated_429_residues
MATRTILVDYLARVEGEGALDIEVHDGRVTSARLRIFEPPRFFEAFLRGRGYAETPDLVARICGICPIAYQMSAVHAIEN
AFGVTVSGQLRALRRLIYCGEWIESHALHIVMLHAPDFLGFPDGIRMASEQGDAVRNGLALKKTGNALMRLMGGREIHPV
NVKTGGFYRVPSRAELEPIGESLERARALAVGLVRWVSSFPFPDYERDYEFVALRHPDEYPFNEGRLVSNRGIDIDIADY
ETEFEERQVAHSTALHALTRRRGAYLVGPLARYALNFDRLPASVQALARGAGLPPVCKNPFQSIIVRALEIVYACEEALK
LIAAYEPPECAAVTVMPRAGTGFGCTEAPRGLLWHRYEFAPDGTIERARIVPPTSQNQPSIEADLAAVASMILDQPDDVI
RARCEQAIRNYDPCISCSAHFLKLSVRRT
>Mature_428_residues
ATRTILVDYLARVEGEGALDIEVHDGRVTSARLRIFEPPRFFEAFLRGRGYAETPDLVARICGICPIAYQMSAVHAIENA
FGVTVSGQLRALRRLIYCGEWIESHALHIVMLHAPDFLGFPDGIRMASEQGDAVRNGLALKKTGNALMRLMGGREIHPVN
VKTGGFYRVPSRAELEPIGESLERARALAVGLVRWVSSFPFPDYERDYEFVALRHPDEYPFNEGRLVSNRGIDIDIADYE
TEFEERQVAHSTALHALTRRRGAYLVGPLARYALNFDRLPASVQALARGAGLPPVCKNPFQSIIVRALEIVYACEEALKL
IAAYEPPECAAVTVMPRAGTGFGCTEAPRGLLWHRYEFAPDGTIERARIVPPTSQNQPSIEADLAAVASMILDQPDDVIR
ARCEQAIRNYDPCISCSAHFLKLSVRRT

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=125, Percent_Identity=27.2, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 47632; Mature: 47501

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRTILVDYLARVEGEGALDIEVHDGRVTSARLRIFEPPRFFEAFLRGRGYAETPDLVA
CCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEEEECCHHHHHHHHCCCCCCCCHHHHH
RICGICPIAYQMSAVHAIENAFGVTVSGQLRALRRLIYCGEWIESHALHIVMLHAPDFLG
HHHHHCHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
FPDGIRMASEQGDAVRNGLALKKTGNALMRLMGGREIHPVNVKTGGFYRVPSRAELEPIG
CCCHHHHHHCCCHHHHCCEEEEHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCHHHHH
ESLERARALAVGLVRWVSSFPFPDYERDYEFVALRHPDEYPFNEGRLVSNRGIDIDIADY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCEEEECCC
ETEFEERQVAHSTALHALTRRRGAYLVGPLARYALNFDRLPASVQALARGAGLPPVCKNP
HHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCHHCCCHHHHHHHHCCCCCHHHCCH
FQSIIVRALEIVYACEEALKLIAAYEPPECAAVTVMPRAGTGFGCTEAPRGLLWHRYEFA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECC
PDGTIERARIVPPTSQNQPSIEADLAAVASMILDQPDDVIRARCEQAIRNYDPCISCSAH
CCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
FLKLSVRRT
EEEEEEECC
>Mature Secondary Structure 
ATRTILVDYLARVEGEGALDIEVHDGRVTSARLRIFEPPRFFEAFLRGRGYAETPDLVA
CCHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEEEECCHHHHHHHHCCCCCCCCHHHHH
RICGICPIAYQMSAVHAIENAFGVTVSGQLRALRRLIYCGEWIESHALHIVMLHAPDFLG
HHHHHCHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
FPDGIRMASEQGDAVRNGLALKKTGNALMRLMGGREIHPVNVKTGGFYRVPSRAELEPIG
CCCHHHHHHCCCHHHHCCEEEEHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCHHHHH
ESLERARALAVGLVRWVSSFPFPDYERDYEFVALRHPDEYPFNEGRLVSNRGIDIDIADY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCEEEECCC
ETEFEERQVAHSTALHALTRRRGAYLVGPLARYALNFDRLPASVQALARGAGLPPVCKNP
HHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCHHCCCHHHHHHHHCCCCCHHHCCH
FQSIIVRALEIVYACEEALKLIAAYEPPECAAVTVMPRAGTGFGCTEAPRGLLWHRYEFA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECC
PDGTIERARIVPPTSQNQPSIEADLAAVASMILDQPDDVIRARCEQAIRNYDPCISCSAH
CCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCE
FLKLSVRRT
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]