Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 182677492

GI number: 182677492

Start: 554349

End: 554930

Strand: Direct

Name: def [H]

Synonym: Bind_0496

Alternate gene names: 182677492

Gene position: 554349-554930 (Clockwise)

Preceding gene: 182677491

Following gene: 182677493

Centisome position: 13.29

GC content: 57.56

Gene sequence:

>582_bases
ATGGCTTTGCGTTCGATCATCACCATTCCCGATCCCCTTTTGCGGCGTGTCTGTGACCCGGTTGCCGATGTCGATTCCGA
GATCCGGCGGCTGATGGATGACATGCTCGAAACCATGTATGACGCGCCAGGTATCGGCTTGGCCGCCAGCCAGATCGCGG
TCATGAAACGGGTCATCGTCATGGATGTCGCCAAACGGCGCAAAGGTGAGGATGGCGCCGAAGCCGATGTCGCGCCTCAG
CCTTTGGCGCTCGCCAATCCGGAAATCCTCTGGGCCTCGGAAGAGCTTTCGACCTATGAAGAGGGTTGCCTGTCCATCCC
CGATTATTACGAGGAAGTGGTTCGTCCGGCCCGCGTCCGTGTCGGCTATCTCGATCGCCAGGGGCAGCGGCAGGAATTGG
AAGCCGATGGCATTCTTGCGACCTGCGTGCAGCATGAGATCGACCATCTGAACGGCGTGCTCTTCATCGATCATATTTCC
CGTCTGAAACGGGAGAGGATCGTCAAGAAATTCAGCAAAGCCGCCAAACGGGACAATCATTCCGCTCCGAAAGAAAAGAG
CAAGGCGGATAGCGAAGCCTGA

Upstream 100 bases:

>100_bases
TTCCGGGGCCGGACGTGAGGCGGGCCGATGATTTTGGCGTAAGTCTGGCATATATAGCGGGATAATTGACCTGATTACCT
CTTCGGATTATCTATTTGCC

Downstream 100 bases:

>100_bases
GTTTTCCGAGTGACTGATCCGCTTCCGACGTAACGAGCGAAGCTCAAAGACGAAGGTTTCCCTTGCGCGTCGTGTTTATG
GGAACGCCGGTTTTTTCCGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MALRSIITIPDPLLRRVCDPVADVDSEIRRLMDDMLETMYDAPGIGLAASQIAVMKRVIVMDVAKRRKGEDGAEADVAPQ
PLALANPEILWASEELSTYEEGCLSIPDYYEEVVRPARVRVGYLDRQGQRQELEADGILATCVQHEIDHLNGVLFIDHIS
RLKRERIVKKFSKAAKRDNHSAPKEKSKADSEA

Sequences:

>Translated_193_residues
MALRSIITIPDPLLRRVCDPVADVDSEIRRLMDDMLETMYDAPGIGLAASQIAVMKRVIVMDVAKRRKGEDGAEADVAPQ
PLALANPEILWASEELSTYEEGCLSIPDYYEEVVRPARVRVGYLDRQGQRQELEADGILATCVQHEIDHLNGVLFIDHIS
RLKRERIVKKFSKAAKRDNHSAPKEKSKADSEA
>Mature_192_residues
ALRSIITIPDPLLRRVCDPVADVDSEIRRLMDDMLETMYDAPGIGLAASQIAVMKRVIVMDVAKRRKGEDGAEADVAPQP
LALANPEILWASEELSTYEEGCLSIPDYYEEVVRPARVRVGYLDRQGQRQELEADGILATCVQHEIDHLNGVLFIDHISR
LKRERIVKKFSKAAKRDNHSAPKEKSKADSEA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1789682, Length=173, Percent_Identity=47.3988439306358, Blast_Score=154, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=34.5679012345679, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=32.9192546583851, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21622; Mature: 21491

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRSIITIPDPLLRRVCDPVADVDSEIRRLMDDMLETMYDAPGIGLAASQIAVMKRVIV
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MDVAKRRKGEDGAEADVAPQPLALANPEILWASEELSTYEEGCLSIPDYYEEVVRPARVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGYLDRQGQRQELEADGILATCVQHEIDHLNGVLFIDHISRLKRERIVKKFSKAAKRDNH
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAPKEKSKADSEA
CCCCHHCCCCCCC
>Mature Secondary Structure 
ALRSIITIPDPLLRRVCDPVADVDSEIRRLMDDMLETMYDAPGIGLAASQIAVMKRVIV
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MDVAKRRKGEDGAEADVAPQPLALANPEILWASEELSTYEEGCLSIPDYYEEVVRPARVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGYLDRQGQRQELEADGILATCVQHEIDHLNGVLFIDHISRLKRERIVKKFSKAAKRDNH
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAPKEKSKADSEA
CCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA