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The map label for this gene is nifK [H]

Identifier: 182677471

GI number: 182677471

Start: 536398

End: 537960

Strand: Direct

Name: nifK [H]

Synonym: Bind_0475

Alternate gene names: 182677471

Gene position: 536398-537960 (Clockwise)

Preceding gene: 182677470

Following gene: 182677472

Centisome position: 12.86

GC content: 56.69

Gene sequence:

>1563_bases
ATGCCTCAGAACGCCGATCACGTTCTCGATCACTTCGACTTGTTCCGTCAGCCGGAATATCGGGAGATGCTCGCGAACAA
GAAGAAGAATTTCGAAAACCCGGTCGCGGAAGCCGAAGTGCAGCGGGTTGTCGAGTGGACCAAGACCCCTGAATATCGTG
AAAAGAACTTTGCGCGTGAGGCGCTGACCGTCAATCCGGCTAAGGCTTGCCAGCCGCTCGGCGCCGTTTTCGCGGCTGTC
GGCTTCGAAGGCACAATTCCCTTCGTTCATGGCTCGCAGGGTTGCGTCGCTTATTATCGTTCGCATTTCTCGCGTCATTT
CAAGGAGCCCAGCTCCTGCGTTTCTTCCTCGATGACGGAAGACGCGGCCGTGTTCGGTGGCCTGAACAACATGATCGACG
GCCTTGCGAACACCTATAACCTTTACAAGCCGAAGATGATTGCCGTTTCGACGACCTGCATGGCGGAAGTCATCGGCGAC
GATCTGAACGCCTTCATCAAGACCGCGAAGGACAAGGGCTCGCTCCCGATGTCCTATGATGTTCCCTTCGCCCACACCCC
GGCCTTCGTCGGCAGCCATATCACGGGCTATGACAATGCGCTGAAGGGCGTGCATGAGCATTTCTGGGGCGGCAAGGCCG
GCACCGTTGAAAAGCTGCAGCGCGTTCCGAACGAAACGATCAACTTCATCGGCGGCTTCGACCCCTACACGGTCGGTAAC
CTCCGTGAGATCCGTCGCATCTTCGACATGATGGGCTTCGAATATACCATCATCGCCGATAACAGCGATGTTTGGGATAC
GCCCACCGACGGCACGTTCCGCATGTTCGATGGCGGCACCACCATCGACGAGATGGTTGCCGCGGTGAACTCGAAGGCCA
CCGTTTCGATGCAGGAATATTGCACCGAAAAGACGATGAAGTACATCGAGGAAGAATGGAAGCACGAGACGGTTGCTTTC
AATCATCCGATCGGTGTCAAGGCGACGGACGATTTCCTTCTCGAAATCTCTCGCCTGACCGGCAAGGCCGTTCCCGAGCA
GATCAAGCTCGAGCGTGGTCGTCTCGTCGACGCGATGGCTGACTCCAGCTCGCATATCCATGGCAAAAAGTTCGCCATCT
ACGGTGATCCCGATCTCTGCATCGGTCTCGCTCAATTCCTGATGGAAAATGGCGGTGAACCGACCCACATTCTGGCGACC
AATGGTTCGCCGAAATGGGCCGAAAAGGTTCAGGCCATGCTCGATGCGAACCCCTTCGGCAAGAACTGCCATGTCTATCC
GCTGCGCGACCTGTGGCACATGCGTTCGCTGCTCTTCACGGAGCCGGTGGACTTCCTGATCGGCAACACCTACGGCAAGT
ATCTGGAGCGTGATACGGGCACCCCGCTGATCCGCATCGGCTTCCCGATCTTCGATCGCCACCATCACCATCGTTATCCG
GTGTGGGGCTATCAGGGTGGTCTCAACGTTCTGGTCTGGATCCTCGACAAGATCTTCGAGGAAATCGACAAGAACACGAT
CGTGCCGGCCCACTCGGACTTCAGCTACGACATCATTCGCTAA

Upstream 100 bases:

>100_bases
CCAAGGCTCCGTTCTAATATCTCCCCGTGCCCTCCTCCCCACGGGAGGAGGGCATTTGTCGGGGTGCACCGAGACAAATC
AAAAAAAGGGATTTTCCAGC

Downstream 100 bases:

>100_bases
GCACAAAACTCAGGAGAACAGCGCAAGCTGTTCTCCTTTGGTTTTAGGGCCCTGTTTTTCCAAACTTGTGGGCGCCTCTA
AAAACCTTCCAAAATGCGGA

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MPQNADHVLDHFDLFRQPEYREMLANKKKNFENPVAEAEVQRVVEWTKTPEYREKNFAREALTVNPAKACQPLGAVFAAV
GFEGTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYNLYKPKMIAVSTTCMAEVIGD
DLNAFIKTAKDKGSLPMSYDVPFAHTPAFVGSHITGYDNALKGVHEHFWGGKAGTVEKLQRVPNETINFIGGFDPYTVGN
LREIRRIFDMMGFEYTIIADNSDVWDTPTDGTFRMFDGGTTIDEMVAAVNSKATVSMQEYCTEKTMKYIEEEWKHETVAF
NHPIGVKATDDFLLEISRLTGKAVPEQIKLERGRLVDAMADSSSHIHGKKFAIYGDPDLCIGLAQFLMENGGEPTHILAT
NGSPKWAEKVQAMLDANPFGKNCHVYPLRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRYP
VWGYQGGLNVLVWILDKIFEEIDKNTIVPAHSDFSYDIIR

Sequences:

>Translated_520_residues
MPQNADHVLDHFDLFRQPEYREMLANKKKNFENPVAEAEVQRVVEWTKTPEYREKNFAREALTVNPAKACQPLGAVFAAV
GFEGTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYNLYKPKMIAVSTTCMAEVIGD
DLNAFIKTAKDKGSLPMSYDVPFAHTPAFVGSHITGYDNALKGVHEHFWGGKAGTVEKLQRVPNETINFIGGFDPYTVGN
LREIRRIFDMMGFEYTIIADNSDVWDTPTDGTFRMFDGGTTIDEMVAAVNSKATVSMQEYCTEKTMKYIEEEWKHETVAF
NHPIGVKATDDFLLEISRLTGKAVPEQIKLERGRLVDAMADSSSHIHGKKFAIYGDPDLCIGLAQFLMENGGEPTHILAT
NGSPKWAEKVQAMLDANPFGKNCHVYPLRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRYP
VWGYQGGLNVLVWILDKIFEEIDKNTIVPAHSDFSYDIIR
>Mature_519_residues
PQNADHVLDHFDLFRQPEYREMLANKKKNFENPVAEAEVQRVVEWTKTPEYREKNFAREALTVNPAKACQPLGAVFAAVG
FEGTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYNLYKPKMIAVSTTCMAEVIGDD
LNAFIKTAKDKGSLPMSYDVPFAHTPAFVGSHITGYDNALKGVHEHFWGGKAGTVEKLQRVPNETINFIGGFDPYTVGNL
REIRRIFDMMGFEYTIIADNSDVWDTPTDGTFRMFDGGTTIDEMVAAVNSKATVSMQEYCTEKTMKYIEEEWKHETVAFN
HPIGVKATDDFLLEISRLTGKAVPEQIKLERGRLVDAMADSSSHIHGKKFAIYGDPDLCIGLAQFLMENGGEPTHILATN
GSPKWAEKVQAMLDANPFGKNCHVYPLRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRYPV
WGYQGGLNVLVWILDKIFEEIDKNTIVPAHSDFSYDIIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 58591; Mature: 58460

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNADHVLDHFDLFRQPEYREMLANKKKNFENPVAEAEVQRVVEWTKTPEYREKNFARE
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFAAVGFEGTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANTYNLYKPKMIAVSTTCMAEVIGDDLNAFIKTAKDKGSLPMSYD
HHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
VPFAHTPAFVGSHITGYDNALKGVHEHFWGGKAGTVEKLQRVPNETINFIGGFDPYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCC
LREIRRIFDMMGFEYTIIADNSDVWDTPTDGTFRMFDGGTTIDEMVAAVNSKATVSMQEY
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEHHHH
CTEKTMKYIEEEWKHETVAFNHPIGVKATDDFLLEISRLTGKAVPEQIKLERGRLVDAMA
HHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHC
DSSSHIHGKKFAIYGDPDLCIGLAQFLMENGGEPTHILATNGSPKWAEKVQAMLDANPFG
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCC
KNCHVYPLRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRYP
CCCEEEEHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHCCCCCCCCC
VWGYQGGLNVLVWILDKIFEEIDKNTIVPAHSDFSYDIIR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQNADHVLDHFDLFRQPEYREMLANKKKNFENPVAEAEVQRVVEWTKTPEYREKNFARE
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFAAVGFEGTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANTYNLYKPKMIAVSTTCMAEVIGDDLNAFIKTAKDKGSLPMSYD
HHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
VPFAHTPAFVGSHITGYDNALKGVHEHFWGGKAGTVEKLQRVPNETINFIGGFDPYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCC
LREIRRIFDMMGFEYTIIADNSDVWDTPTDGTFRMFDGGTTIDEMVAAVNSKATVSMQEY
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEHHHH
CTEKTMKYIEEEWKHETVAFNHPIGVKATDDFLLEISRLTGKAVPEQIKLERGRLVDAMA
HHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHC
DSSSHIHGKKFAIYGDPDLCIGLAQFLMENGGEPTHILATNGSPKWAEKVQAMLDANPFG
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCC
KNCHVYPLRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRYP
CCCEEEEHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHCCCCCCCCC
VWGYQGGLNVLVWILDKIFEEIDKNTIVPAHSDFSYDIIR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275 [H]