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The map label for this gene is nifD [H]

Identifier: 182677470

GI number: 182677470

Start: 534854

End: 536314

Strand: Direct

Name: nifD [H]

Synonym: Bind_0474

Alternate gene names: 182677470

Gene position: 534854-536314 (Clockwise)

Preceding gene: 182677469

Following gene: 182677471

Centisome position: 12.83

GC content: 56.13

Gene sequence:

>1461_bases
ATGAGCCTATCGGCACCCGAAACAATCGAAGAAATCAAGCAGAGGAACAAGGAACTCATCGCTGAGGTCCTGGAGGCCTA
TCCCGAAAAGAGCAAGAAAAACCGCGCCAAGCACCTCAACCAGTTCCAGGAAGGTGGCAAGGATTGCTCGGTCAAGTCCA
ACATCAAGTCCGTCCCGGGCGTGATGACGATCCGTGGCTGCGCCTACGCCGGTTCGAAGGGCGTGGTGTGGGGTCCGATC
AAGGACATGATCCACATCAGCCATGGTCCGGTCGGCTGCGGCCAGTACTCCTGGGCTGCGCGTCGTAACTATTACATTGG
CACGACCGGTGTTGACACCTTCGTGACCATGCAGTTCACCTCGGACTTCCAGGAAAAGGACATTGTCTTCGGCGGCGACA
AGAAGCTCGCGAAGATCATGGACGAAATCATGGAATTGTTCCCCCTGAACCATGGTGTCACGGTTCAGTCGGAATGCCCG
ATCGGCCTCATCGGTGACGACATCGAAGCCGTTTCGAAGCAGAAGTCCAAGGAATATGGCGGCAAGACCATTGTTCCGGT
CCGCTGCGAAGGCTTCCGTGGCGTTTCCCAGTCTCTCGGCCACCACATTGCGAACGACGCCGTTCGTGACTGGGTGTTCG
ACAAGATGGAAGGCAAGCCCGCCCGTATCGAACTCACCGACTATGACGTTGCCATCATCGGCGACTACAACATCGGTGGT
GACGCTTGGTCGTCCCGTATCCTTCTCGAGGAAATGGGCCTCCGCGTGATCGCTCAGTGGTCGGGTGACGGTTCCATCGC
CGAACTCGAGGCGACGCCGAAGGCGAAGCTCAACGTCCTTCACTGCTACCGCTCGATGAACTACATCTCCCGCCACATGG
AAGAAAAGTACGGTGTTCCGTGGGTGGAATATAACTTCTTCGGCCCGTCCAAGATCGCTGAGTCGCTGCGCACGATCGCC
AGCCACTTCGACGACAAGATCAAGGAAAATGCCGAGAAGGTCATCGCCAAGTATCGCGCTCTGTCCGATGCGGTGATCGA
GAAGTATCGTCCGCGTCTCCATGGCCGTAAGGTCATGCTCTTCGTCGGCGGTCTGCGTCCGCGTCACGTTATCGGCGCTT
ACGAAGATCTCGGCATGGAAGTTGTCGGTACCGGCTATGAGTTCGGCCATAACGACGACTATCAGCGCACCACTCACTAT
GTGAAGGACGGCACGCTGATCTATGACGACGTGACCGGCTACGAGTTCGAGAAGTTCGTCGAAAAGATCCAGCCTGATCT
GGTTGGTTCCGGCATTAAGGAAAAGTACGTCTTCCAGAAAATGGGCGTTCCTTTCCGTCAGATGCATTCTTGGGACTATT
CGGGCCCGTATCATGGCTATGATGGCTTCGCCATCTTCGCTCGCGATATGGACATGGCCATCAATTCCCCGGTTTGGGGT
TTGACCAAGGCTCCGTTCTAA

Upstream 100 bases:

>100_bases
CGGCCTCACCGCCGCTCAGCTCTCGGCCTAATGCCCTTAAGCCGGCCCCTCTCAGGAGGGGCCGGTCTTTACTGCAAGAC
GAATACTTCAGGAGATTGGC

Downstream 100 bases:

>100_bases
TATCTCCCCGTGCCCTCCTCCCCACGGGAGGAGGGCATTTGTCGGGGTGCACCGAGACAAATCAAAAAAAGGGATTTTCC
AGCATGCCTCAGAACGCCGA

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSLSAPETIEEIKQRNKELIAEVLEAYPEKSKKNRAKHLNQFQEGGKDCSVKSNIKSVPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIMELFPLNHGVTVQSECP
IGLIGDDIEAVSKQKSKEYGGKTIVPVRCEGFRGVSQSLGHHIANDAVRDWVFDKMEGKPARIELTDYDVAIIGDYNIGG
DAWSSRILLEEMGLRVIAQWSGDGSIAELEATPKAKLNVLHCYRSMNYISRHMEEKYGVPWVEYNFFGPSKIAESLRTIA
SHFDDKIKENAEKVIAKYRALSDAVIEKYRPRLHGRKVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHY
VKDGTLIYDDVTGYEFEKFVEKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWG
LTKAPF

Sequences:

>Translated_486_residues
MSLSAPETIEEIKQRNKELIAEVLEAYPEKSKKNRAKHLNQFQEGGKDCSVKSNIKSVPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIMELFPLNHGVTVQSECP
IGLIGDDIEAVSKQKSKEYGGKTIVPVRCEGFRGVSQSLGHHIANDAVRDWVFDKMEGKPARIELTDYDVAIIGDYNIGG
DAWSSRILLEEMGLRVIAQWSGDGSIAELEATPKAKLNVLHCYRSMNYISRHMEEKYGVPWVEYNFFGPSKIAESLRTIA
SHFDDKIKENAEKVIAKYRALSDAVIEKYRPRLHGRKVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHY
VKDGTLIYDDVTGYEFEKFVEKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWG
LTKAPF
>Mature_485_residues
SLSAPETIEEIKQRNKELIAEVLEAYPEKSKKNRAKHLNQFQEGGKDCSVKSNIKSVPGVMTIRGCAYAGSKGVVWGPIK
DMIHISHGPVGCGQYSWAARRNYYIGTTGVDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIMELFPLNHGVTVQSECPI
GLIGDDIEAVSKQKSKEYGGKTIVPVRCEGFRGVSQSLGHHIANDAVRDWVFDKMEGKPARIELTDYDVAIIGDYNIGGD
AWSSRILLEEMGLRVIAQWSGDGSIAELEATPKAKLNVLHCYRSMNYISRHMEEKYGVPWVEYNFFGPSKIAESLRTIAS
HFDDKIKENAEKVIAKYRALSDAVIEKYRPRLHGRKVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYV
KDGTLIYDDVTGYEFEKFVEKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWGL
TKAPF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54716; Mature: 54585

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSAPETIEEIKQRNKELIAEVLEAYPEKSKKNRAKHLNQFQEGGKDCSVKSNIKSVPG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGVDTFVTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIMELFPLNHGVTVQSECPIGLIGDDIEAVSKQKSKEYG
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCC
GKTIVPVRCEGFRGVSQSLGHHIANDAVRDWVFDKMEGKPARIELTDYDVAIIGDYNIGG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCC
DAWSSRILLEEMGLRVIAQWSGDGSIAELEATPKAKLNVLHCYRSMNYISRHMEEKYGVP
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
WVEYNFFGPSKIAESLRTIASHFDDKIKENAEKVIAKYRALSDAVIEKYRPRLHGRKVML
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFV
EECCCCCHHHCCCHHHCCCEEEECCEECCCCCCHHHHHHEEECCEEEEECCCCCHHHHHH
EKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWG
HHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCCCCC
LTKAPF
CCCCCC
>Mature Secondary Structure 
SLSAPETIEEIKQRNKELIAEVLEAYPEKSKKNRAKHLNQFQEGGKDCSVKSNIKSVPG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGVDTFVTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIMELFPLNHGVTVQSECPIGLIGDDIEAVSKQKSKEYG
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCC
GKTIVPVRCEGFRGVSQSLGHHIANDAVRDWVFDKMEGKPARIELTDYDVAIIGDYNIGG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCC
DAWSSRILLEEMGLRVIAQWSGDGSIAELEATPKAKLNVLHCYRSMNYISRHMEEKYGVP
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
WVEYNFFGPSKIAESLRTIASHFDDKIKENAEKVIAKYRALSDAVIEKYRPRLHGRKVML
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFV
EECCCCCHHHCCCHHHCCCEEEECCEECCCCCCHHHHHHEEECCEEEEECCCCCHHHHHH
EKIQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWG
HHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCCCCC
LTKAPF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275; 6588133 [H]