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The map label for this gene is mutM

Identifier: 182677448

GI number: 182677448

Start: 508434

End: 509300

Strand: Direct

Name: mutM

Synonym: Bind_0452

Alternate gene names: 182677448

Gene position: 508434-509300 (Clockwise)

Preceding gene: 182677444

Following gene: 182677459

Centisome position: 12.19

GC content: 62.4

Gene sequence:

>867_bases
ATGCCCGAATTGCCTGAAGTCGAAACCGTCCGCCGGGGGCTGGCGCCGGTCATGGTCGGGGCTTCCTTCACGACTGTAGA
GCAGCGACGGGCCGATCTTCGCTTTCCCTTTCCGGACAATTTCGCGGCTCGCCTTGAGGGTCGCCGTGTGGAAGCGCTCG
GCCGCCGCGCCAAATATCTGCTCGCCGATCTCGATGATGCGCAAGTGCTGGTCATGCATCTCGGCATGTCCGGTTCGTTT
CGCATCGAGAAGGCGGGCGATCTCGCCTCCCCGCCTCCTGGCAAGAACGCTGCGCATGATCATGTGGTCTTCGGGCTTTC
GACGGGCACGCGCATCATTTACAATGATCCACGCCGTTTCGGTTTCATGCATTTGATCGCGCGCCAGGACCTGGCGGGGC
ATCCTTTGTTCCGCAATGTCGGCATCGAGCCGCTCGGCAATGAGCTCGAAGGGGCCTTGCTGGCGCGATTGTTTGCAGGC
AAGACGACGCCGCTCAAGACCGCGCTGCTCGATCAGACCCTGATTGCCGGCCTCGGCAATATTTATGTATGCGAGGCTTT
GCACCGGGCGGGGCTTTCACCGCGCCGGGCGGCGGGGACGCTCGCCGGCAAGAAGGGCCAGCCGACAGAGCGCGCGCATC
GTCTTTCCGAAATCATCCGCGCGGTTTTGGAAGAAGCGATCGAGGCGGGCGGTTCATCCTTGCGCGATCATCGCCAAGCC
GATGGCGCGCTCGGCTATTTCCAGCACCGTTTCCGTGTCTATGATCGGGAAGCAGAGCCCTGCCCGCGAGAGGGGTGTGG
TGGCACGATCAAGCGTATCGTGCAGGCCGGACGATCGACGTTCTTTTGTGCCAAATGCCAGCGGTGA

Upstream 100 bases:

>100_bases
GATGCGGCACGTGGCATGCCCATGAATTTTATCCTATCTATCAAATCTCGCGGGCTCACGAGAGTGAGGCTGTGCTGACG
ATTGGTTACAAGGTTGTAAC

Downstream 100 bases:

>100_bases
GACTCGTGGAAGCGATCCATGCCATTGCATGTTCAAGGCAAGGAACCGCTTCGGAGTGACGGGCGTGATGAATGACAGTC
ACTGACACTTACAGCCGTGC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPELPEVETVRRGLAPVMVGASFTTVEQRRADLRFPFPDNFAARLEGRRVEALGRRAKYLLADLDDAQVLVMHLGMSGSF
RIEKAGDLASPPPGKNAAHDHVVFGLSTGTRIIYNDPRRFGFMHLIARQDLAGHPLFRNVGIEPLGNELEGALLARLFAG
KTTPLKTALLDQTLIAGLGNIYVCEALHRAGLSPRRAAGTLAGKKGQPTERAHRLSEIIRAVLEEAIEAGGSSLRDHRQA
DGALGYFQHRFRVYDREAEPCPREGCGGTIKRIVQAGRSTFFCAKCQR

Sequences:

>Translated_288_residues
MPELPEVETVRRGLAPVMVGASFTTVEQRRADLRFPFPDNFAARLEGRRVEALGRRAKYLLADLDDAQVLVMHLGMSGSF
RIEKAGDLASPPPGKNAAHDHVVFGLSTGTRIIYNDPRRFGFMHLIARQDLAGHPLFRNVGIEPLGNELEGALLARLFAG
KTTPLKTALLDQTLIAGLGNIYVCEALHRAGLSPRRAAGTLAGKKGQPTERAHRLSEIIRAVLEEAIEAGGSSLRDHRQA
DGALGYFQHRFRVYDREAEPCPREGCGGTIKRIVQAGRSTFFCAKCQR
>Mature_287_residues
PELPEVETVRRGLAPVMVGASFTTVEQRRADLRFPFPDNFAARLEGRRVEALGRRAKYLLADLDDAQVLVMHLGMSGSFR
IEKAGDLASPPPGKNAAHDHVVFGLSTGTRIIYNDPRRFGFMHLIARQDLAGHPLFRNVGIEPLGNELEGALLARLFAGK
TTPLKTALLDQTLIAGLGNIYVCEALHRAGLSPRRAAGTLAGKKGQPTERAHRLSEIIRAVLEEAIEAGGSSLRDHRQAD
GALGYFQHRFRVYDREAEPCPREGCGGTIKRIVQAGRSTFFCAKCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=288, Percent_Identity=40.9722222222222, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786932, Length=292, Percent_Identity=27.0547945205479, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_BEII9 (B2IE81)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831594.1
- GeneID:   6199221
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0452
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31573; Mature: 31442

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 278-278 BINDING 99-99 BINDING 118-118 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRGLAPVMVGASFTTVEQRRADLRFPFPDNFAARLEGRRVEALGRRAKYL
CCCCCCHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LADLDDAQVLVMHLGMSGSFRIEKAGDLASPPPGKNAAHDHVVFGLSTGTRIIYNDPRRF
HHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHH
GFMHLIARQDLAGHPLFRNVGIEPLGNELEGALLARLFAGKTTPLKTALLDQTLIAGLGN
HHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
IYVCEALHRAGLSPRRAAGTLAGKKGQPTERAHRLSEIIRAVLEEAIEAGGSSLRDHRQA
HHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DGALGYFQHRFRVYDREAEPCPREGCGGTIKRIVQAGRSTFFCAKCQR
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
PELPEVETVRRGLAPVMVGASFTTVEQRRADLRFPFPDNFAARLEGRRVEALGRRAKYL
CCCCCHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
LADLDDAQVLVMHLGMSGSFRIEKAGDLASPPPGKNAAHDHVVFGLSTGTRIIYNDPRRF
HHCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHH
GFMHLIARQDLAGHPLFRNVGIEPLGNELEGALLARLFAGKTTPLKTALLDQTLIAGLGN
HHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
IYVCEALHRAGLSPRRAAGTLAGKKGQPTERAHRLSEIIRAVLEEAIEAGGSSLRDHRQA
HHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DGALGYFQHRFRVYDREAEPCPREGCGGTIKRIVQAGRSTFFCAKCQR
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA