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The map label for this gene is gltX [H]

Identifier: 182677429

GI number: 182677429

Start: 487082

End: 487984

Strand: Direct

Name: gltX [H]

Synonym: Bind_0433

Alternate gene names: 182677429

Gene position: 487082-487984 (Clockwise)

Preceding gene: 182677427

Following gene: 182677431

Centisome position: 11.68

GC content: 57.7

Gene sequence:

>903_bases
ATGACCCACGCCTGTCCTCAGTCTGCCTTTCGCTTCGCTCCCTCGCCCAATGGCTATCTGCATCTCGGTCACGCTTTTTC
GGCGCTCGAAAATGAGCGTCTGGCGCGTTTGAACGGGGGCCGTCTCTTCTTGCGAATCGAAAATATCGATGTCGATCGCT
GCCGGCCGGAATATGAACGCGCGATTCTCGAGGATCTGACCTGGCTTGGTATCGCGCTGGAGCCCGAAGTGCGGCGGCAG
TCCGAGCATTTCGACGATTATGCACAAGCCCTGGACCGGCTCGCGCGGATGGGCCTCATCTATCCCTGTTTCTGTTCCCG
TACTGACATCATGCAGAAGATACGGGAACGGGCCTTTTGGGCGCGCGATCCTGACGGATCGCCGCTTTATCCCGGCACCT
GCAAACATCTTCCAAAGGACAAAATCCAGGAGCGCCTGGAGGCTGGCGAGCCGGCCTCTTATCGCCTCGACATGGGCAAA
GCCTTGAATCTGATTGGTTATAGGCTCGGCTGGACCGAATTTGGTTCCGGGATGGTCGGTCGCTTCGTACAGGCTAGTCC
GCAGAAATGGGGGGATACCATTCTGGCCCGCAAGGATATTGCGACGAGCTATCACATTGCCGTCGTCGTTGACGATGCCT
TGCAACGGATCACTGATATCGTACGCGGCGAAGATTTGTTCCAGGCGACCAGTCTGCACCGGCTGTTGCAAAGCCTGTTG
GGCTTGCCTGAACCGGCCTATCACCATCACGGGCTGCTGCGTGATGCCTCTGGCCAGAAATTGTCGAAAAGTCTCAGAGC
CAAATCCTTGCGTGCCTTGCGCGAGGAAGGATTATCCCCGAAAGCAGTGCGCGCCAAACTGGCGCCACTGCCCCCTCTCA
TCGCCGCTTTGCAAACGGTTTGA

Upstream 100 bases:

>100_bases
ATCCGATTTCGGCTGAATGAGGGATGCGTGAGCTTGGCTCGTTGCCATTTCGAATTCTGGCCGGGATGTTTCCTGCCTCT
GTCCGTCCCCGAAGTAAGCC

Downstream 100 bases:

>100_bases
AATCAAGCCGTTTGAATCGGTCGCTATTCAGCGGCGCTTTTGGCGCCGCCGAAACGCCGCTCGATATAATCCTGAACGAT
GGTCTTGAATTCGGCGGCTA

Product: glutamyl-tRNA synthetase class Ic

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTHACPQSAFRFAPSPNGYLHLGHAFSALENERLARLNGGRLFLRIENIDVDRCRPEYERAILEDLTWLGIALEPEVRRQ
SEHFDDYAQALDRLARMGLIYPCFCSRTDIMQKIRERAFWARDPDGSPLYPGTCKHLPKDKIQERLEAGEPASYRLDMGK
ALNLIGYRLGWTEFGSGMVGRFVQASPQKWGDTILARKDIATSYHIAVVVDDALQRITDIVRGEDLFQATSLHRLLQSLL
GLPEPAYHHHGLLRDASGQKLSKSLRAKSLRALREEGLSPKAVRAKLAPLPPLIAALQTV

Sequences:

>Translated_300_residues
MTHACPQSAFRFAPSPNGYLHLGHAFSALENERLARLNGGRLFLRIENIDVDRCRPEYERAILEDLTWLGIALEPEVRRQ
SEHFDDYAQALDRLARMGLIYPCFCSRTDIMQKIRERAFWARDPDGSPLYPGTCKHLPKDKIQERLEAGEPASYRLDMGK
ALNLIGYRLGWTEFGSGMVGRFVQASPQKWGDTILARKDIATSYHIAVVVDDALQRITDIVRGEDLFQATSLHRLLQSLL
GLPEPAYHHHGLLRDASGQKLSKSLRAKSLRALREEGLSPKAVRAKLAPLPPLIAALQTV
>Mature_299_residues
THACPQSAFRFAPSPNGYLHLGHAFSALENERLARLNGGRLFLRIENIDVDRCRPEYERAILEDLTWLGIALEPEVRRQS
EHFDDYAQALDRLARMGLIYPCFCSRTDIMQKIRERAFWARDPDGSPLYPGTCKHLPKDKIQERLEAGEPASYRLDMGKA
LNLIGYRLGWTEFGSGMVGRFVQASPQKWGDTILARKDIATSYHIAVVVDDALQRITDIVRGEDLFQATSLHRLLQSLLG
LPEPAYHHHGLLRDASGQKLSKSLRAKSLRALREEGLSPKAVRAKLAPLPPLIAALQTV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=286, Percent_Identity=30.4195804195804, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1788743, Length=280, Percent_Identity=32.1428571428571, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI226510925, Length=290, Percent_Identity=33.448275862069, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=284, Percent_Identity=28.5211267605634, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324540, Length=275, Percent_Identity=32.3636363636364, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324742, Length=309, Percent_Identity=23.9482200647249, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=298, Percent_Identity=33.5570469798658, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 33787; Mature: 33656

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHACPQSAFRFAPSPNGYLHLGHAFSALENERLARLNGGRLFLRIENIDVDRCRPEYER
CCCCCCCHHHCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCHHHCCHHHHH
AILEDLTWLGIALEPEVRRQSEHFDDYAQALDRLARMGLIYPCFCSRTDIMQKIRERAFW
HHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCC
ARDPDGSPLYPGTCKHLPKDKIQERLEAGEPASYRLDMGKALNLIGYRLGWTEFGSGMVG
CCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCHHHHCCCHHH
RFVQASPQKWGDTILARKDIATSYHIAVVVDDALQRITDIVRGEDLFQATSLHRLLQSLL
HHHHCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GLPEPAYHHHGLLRDASGQKLSKSLRAKSLRALREEGLSPKAVRAKLAPLPPLIAALQTV
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
THACPQSAFRFAPSPNGYLHLGHAFSALENERLARLNGGRLFLRIENIDVDRCRPEYER
CCCCCCHHHCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCHHHCCHHHHH
AILEDLTWLGIALEPEVRRQSEHFDDYAQALDRLARMGLIYPCFCSRTDIMQKIRERAFW
HHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCC
ARDPDGSPLYPGTCKHLPKDKIQERLEAGEPASYRLDMGKALNLIGYRLGWTEFGSGMVG
CCCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCHHHHCCCHHH
RFVQASPQKWGDTILARKDIATSYHIAVVVDDALQRITDIVRGEDLFQATSLHRLLQSLL
HHHHCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GLPEPAYHHHGLLRDASGQKLSKSLRAKSLRALREEGLSPKAVRAKLAPLPPLIAALQTV
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]