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The map label for this gene is thiL [H]

Identifier: 182677207

GI number: 182677207

Start: 244798

End: 245793

Strand: Direct

Name: thiL [H]

Synonym: Bind_0208

Alternate gene names: 182677207

Gene position: 244798-245793 (Clockwise)

Preceding gene: 182677206

Following gene: 182677208

Centisome position: 5.87

GC content: 64.66

Gene sequence:

>996_bases
ATGCCACGCTTATCCGAGGACGAGCTGATCGCGACTTATTTCGCGCCGCTCGCGGGCCCCGCCGGCCTGAACCTGCGCGA
TGATGCGGCGGTGCTCACTCCGCTTCCTGGCCAGGATCTCGTCCTGACCAAGGACATGCTTGTCGCAGGTGTGCATTTCT
TTCCCGACGATGCGCCGGAGACGATCGCGCGCAAACTGTTGCGCGTCAATCTGTCTGATCTTGCCGCCAAGGCAGCTCTC
CCTTGCGGTTTTCTGCTCGGCCTTGCCTTGCCGCCCGATTGGGGGACGGATTGGCTCGCCGCATTCGCACGCGGGCTTGG
TGAGGATAGCGCGCAATTTCGTTTTCCCTTGCTGGGTGGGGATACGGTCAAGACGCCCGGTCCATTGACCCTCAGCTTGA
CAGCGCTCGGTCACGTCCCGCGCGACACGATGGTGCCACGCCCCGGTGCACGGGCGGGTGACCGGCTTTATGTTACGGGG
ACGATTGGTGATGCTGCGCTTGGCCTGCGCGTGCGTCTCGACGCGCCGCAAGATCGTATCTGGATCGCCGCGCTGACGCC
CGCCGCGCGAACCGATCTCCTCAATCGTTATCTCGTTCCCGAGCCGCGCCTGGGCCTTGCGCCGGCTTTGCGCCGTCACG
CCCGCGCCGCGATGGATGTGTCCGATGGTTTTGCGGGCGATCTCGCCAAACTGCTGGGACTCGCTCATCTTACGGTGCGT
GTCCCGCTTGCCGCGGTGCCGCTCTCCAGGGCCGCGCGGGAAGCCCTCCATCTCGAGCCTGGCCTGATCGATACGATCCT
GACCGGCGGTGATGATTACGAAATCCTGTGCGCCGTGCCGCCGGGGGAATGTGTGGGATTCGAGGAATCGGCGCTGGCGC
TGGGTATGCGTGTCAGCCCGATCGGCGAGGCGGAGAGCGGCGATGCCCTGCCACATTTCGAAGCACCGGGAACTGTGCCG
CGTGTATACCCGCAGGGATCCTTCCAGCATTTCTGA

Upstream 100 bases:

>100_bases
CGCGAGGAATCGGGCATGATCAATGCTGTGCTCGACGAATTGGCCCGCCGTTATCGCGCCGACGAATTCGCCGCCTCGCG
TCTTTAGAATACAGACTGTC

Downstream 100 bases:

>100_bases
GTGATTCCACGCCATTTTATACCAACGGTCATGATCGATGACCTTTGGTATTAGCACTGTGAAACTAGGGCTTTGCCAAT
GATTTCAAGGCCTCCCATTT

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MPRLSEDELIATYFAPLAGPAGLNLRDDAAVLTPLPGQDLVLTKDMLVAGVHFFPDDAPETIARKLLRVNLSDLAAKAAL
PCGFLLGLALPPDWGTDWLAAFARGLGEDSAQFRFPLLGGDTVKTPGPLTLSLTALGHVPRDTMVPRPGARAGDRLYVTG
TIGDAALGLRVRLDAPQDRIWIAALTPAARTDLLNRYLVPEPRLGLAPALRRHARAAMDVSDGFAGDLAKLLGLAHLTVR
VPLAAVPLSRAAREALHLEPGLIDTILTGGDDYEILCAVPPGECVGFEESALALGMRVSPIGEAESGDALPHFEAPGTVP
RVYPQGSFQHF

Sequences:

>Translated_331_residues
MPRLSEDELIATYFAPLAGPAGLNLRDDAAVLTPLPGQDLVLTKDMLVAGVHFFPDDAPETIARKLLRVNLSDLAAKAAL
PCGFLLGLALPPDWGTDWLAAFARGLGEDSAQFRFPLLGGDTVKTPGPLTLSLTALGHVPRDTMVPRPGARAGDRLYVTG
TIGDAALGLRVRLDAPQDRIWIAALTPAARTDLLNRYLVPEPRLGLAPALRRHARAAMDVSDGFAGDLAKLLGLAHLTVR
VPLAAVPLSRAAREALHLEPGLIDTILTGGDDYEILCAVPPGECVGFEESALALGMRVSPIGEAESGDALPHFEAPGTVP
RVYPQGSFQHF
>Mature_330_residues
PRLSEDELIATYFAPLAGPAGLNLRDDAAVLTPLPGQDLVLTKDMLVAGVHFFPDDAPETIARKLLRVNLSDLAAKAALP
CGFLLGLALPPDWGTDWLAAFARGLGEDSAQFRFPLLGGDTVKTPGPLTLSLTALGHVPRDTMVPRPGARAGDRLYVTGT
IGDAALGLRVRLDAPQDRIWIAALTPAARTDLLNRYLVPEPRLGLAPALRRHARAAMDVSDGFAGDLAKLLGLAHLTVRV
PLAAVPLSRAAREALHLEPGLIDTILTGGDDYEILCAVPPGECVGFEESALALGMRVSPIGEAESGDALPHFEAPGTVPR
VYPQGSFQHF

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=302, Percent_Identity=38.0794701986755, Blast_Score=150, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 35008; Mature: 34877

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLSEDELIATYFAPLAGPAGLNLRDDAAVLTPLPGQDLVLTKDMLVAGVHFFPDDAPE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEHHHHHHCCCCCCCCCHH
TIARKLLRVNLSDLAAKAALPCGFLLGLALPPDWGTDWLAAFARGLGEDSAQFRFPLLGG
HHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECC
DTVKTPGPLTLSLTALGHVPRDTMVPRPGARAGDRLYVTGTIGDAALGLRVRLDAPQDRI
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCE
WIAALTPAARTDLLNRYLVPEPRLGLAPALRRHARAAMDVSDGFAGDLAKLLGLAHLTVR
EEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEE
VPLAAVPLSRAAREALHLEPGLIDTILTGGDDYEILCAVPPGECVGFEESALALGMRVSP
CCHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEECEEECC
IGEAESGDALPHFEAPGTVPRVYPQGSFQHF
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
PRLSEDELIATYFAPLAGPAGLNLRDDAAVLTPLPGQDLVLTKDMLVAGVHFFPDDAPE
CCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEHHHHHHCCCCCCCCCHH
TIARKLLRVNLSDLAAKAALPCGFLLGLALPPDWGTDWLAAFARGLGEDSAQFRFPLLGG
HHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECC
DTVKTPGPLTLSLTALGHVPRDTMVPRPGARAGDRLYVTGTIGDAALGLRVRLDAPQDRI
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCE
WIAALTPAARTDLLNRYLVPEPRLGLAPALRRHARAAMDVSDGFAGDLAKLLGLAHLTVR
EEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEE
VPLAAVPLSRAAREALHLEPGLIDTILTGGDDYEILCAVPPGECVGFEESALALGMRVSP
CCHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEECEEECC
IGEAESGDALPHFEAPGTVPRVYPQGSFQHF
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]