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The map label for this gene is nemA [H]

Identifier: 182677091

GI number: 182677091

Start: 102176

End: 103288

Strand: Direct

Name: nemA [H]

Synonym: Bind_0089

Alternate gene names: 182677091

Gene position: 102176-103288 (Clockwise)

Preceding gene: 182677089

Following gene: 182677092

Centisome position: 2.45

GC content: 60.38

Gene sequence:

>1113_bases
ATGGGCCGCTTGTTCGAACCCTTCCGTCTCGGCAAATATCTCTTGCGCAATCGCGTCGTCATGGCGCCGATGACCCGCGC
CCGCAATCCCGATGGTGTCCCCAATGACCTCAATGCGCTCTATTATCGCCAGCGCGCCAGCGCTGGATTGATCGTGACCG
AAGGTGTGCCGATCTCGCCCAGTGCCGAGGGTTTTCTTTTCATTCCCGGCCTTTTTCGGCCTGACCAGATCATCGGCTGG
CGAAAGGTGACCGATGCGGTCCATGCGGAAGGTGGGACGATCTTCGCTCAGCTCTGGCATGTGGGGCGCGTCAGCCATGT
ATCGAACCAGCCGGGTGGCATTGCGCCGGTCAGTTCGACAGAGCATCCGGCGCTTCATTCCGAGGCCTGGGGTTTGCGTG
ACGATGGCACGCCGGGCGCGGTTCCGGTCTCCACGCCGCGTGCGTTGAGCACGGCGGAAGTCGCGGGCGTGATCGAGGAT
TTTGCCAAAGCGGCGGCCAATGCGATTGCCGCGGGTTTCGATGGTGTCGAATTGCATGGAGCCAATGGCTATCTGATCGA
ACAATTCCTCAATCCCAATGTGAATGATCGCACGGATGCCTATCGCGGCGATACTCTGGAAGGGCGCACCCGCTTCGTGA
TTGAGGCCATTGACGCAGTCATTGCCCGCATTGGTGCGGAGCGCACCGCGATAAGGCTGTCACCCTATGGGGGGCTGTTC
GATATGCAGCCCTATCCGCAAATCGAGGAAACCTATCTTTTCCTCGCCGATGCCTTCAGCCGGCGCGGCCTCGCCTATGT
GCATCTGATGGATCAGAAATCGCGCGGCAGCACGGCCATTCCTCAAAACTTCCTGGCCGAATTCCGCACCTGTTACAAAG
GCGCGCTGATCCTGGCCGGCGGCCTGACACGCGATCGGGCGGAGACGCTGATCGCGGAAGGAATGATTGATCTGGCGGCC
TTTGGCGAGCCTTTCATCGCCAATCCGGATCTCGTCGCGCGGCTGGAAAAAGGTCTTCCCCTCGCTCTGGCCGACCGCTC
GCTTCATTATGGGGGCGGCGCGCATGGCTATACCGATTATTCCGTTTATCACGGCGAAGGGGTCAGGCTATGA

Upstream 100 bases:

>100_bases
CGATCTATGAATATTTTTCACAGGTTTTGGGAAAGGCTGGCGGCTTATCGAGCCGGAGCGCGGCTGTCATCTGTGCGCAT
GAAATCCGAAAGGAGCATCC

Downstream 100 bases:

>100_bases
GGATCTTCGTCACGGGCGCAAGTGGCTATATTGGCGGTTCAGTCGCGGCCGCGCTCGCGCAAAAAGGGCATGTGGTACGG
GGCCTTGTGCGCAATCCCGC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MGRLFEPFRLGKYLLRNRVVMAPMTRARNPDGVPNDLNALYYRQRASAGLIVTEGVPISPSAEGFLFIPGLFRPDQIIGW
RKVTDAVHAEGGTIFAQLWHVGRVSHVSNQPGGIAPVSSTEHPALHSEAWGLRDDGTPGAVPVSTPRALSTAEVAGVIED
FAKAAANAIAAGFDGVELHGANGYLIEQFLNPNVNDRTDAYRGDTLEGRTRFVIEAIDAVIARIGAERTAIRLSPYGGLF
DMQPYPQIEETYLFLADAFSRRGLAYVHLMDQKSRGSTAIPQNFLAEFRTCYKGALILAGGLTRDRAETLIAEGMIDLAA
FGEPFIANPDLVARLEKGLPLALADRSLHYGGGAHGYTDYSVYHGEGVRL

Sequences:

>Translated_370_residues
MGRLFEPFRLGKYLLRNRVVMAPMTRARNPDGVPNDLNALYYRQRASAGLIVTEGVPISPSAEGFLFIPGLFRPDQIIGW
RKVTDAVHAEGGTIFAQLWHVGRVSHVSNQPGGIAPVSSTEHPALHSEAWGLRDDGTPGAVPVSTPRALSTAEVAGVIED
FAKAAANAIAAGFDGVELHGANGYLIEQFLNPNVNDRTDAYRGDTLEGRTRFVIEAIDAVIARIGAERTAIRLSPYGGLF
DMQPYPQIEETYLFLADAFSRRGLAYVHLMDQKSRGSTAIPQNFLAEFRTCYKGALILAGGLTRDRAETLIAEGMIDLAA
FGEPFIANPDLVARLEKGLPLALADRSLHYGGGAHGYTDYSVYHGEGVRL
>Mature_369_residues
GRLFEPFRLGKYLLRNRVVMAPMTRARNPDGVPNDLNALYYRQRASAGLIVTEGVPISPSAEGFLFIPGLFRPDQIIGWR
KVTDAVHAEGGTIFAQLWHVGRVSHVSNQPGGIAPVSSTEHPALHSEAWGLRDDGTPGAVPVSTPRALSTAEVAGVIEDF
AKAAANAIAAGFDGVELHGANGYLIEQFLNPNVNDRTDAYRGDTLEGRTRFVIEAIDAVIARIGAERTAIRLSPYGGLFD
MQPYPQIEETYLFLADAFSRRGLAYVHLMDQKSRGSTAIPQNFLAEFRTCYKGALILAGGLTRDRAETLIAEGMIDLAAF
GEPFIANPDLVARLEKGLPLALADRSLHYGGGAHGYTDYSVYHGEGVRL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=361, Percent_Identity=45.983379501385, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1789463, Length=353, Percent_Identity=31.4447592067989, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=378, Percent_Identity=28.8359788359788, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=364, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=345, Percent_Identity=27.8260869565217, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=28.8629737609329, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=323, Percent_Identity=27.5541795665635, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=218, Percent_Identity=31.651376146789, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=391, Percent_Identity=36.5728900255754, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321973, Length=396, Percent_Identity=34.8484848484849, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40091; Mature: 39960

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLFEPFRLGKYLLRNRVVMAPMTRARNPDGVPNDLNALYYRQRASAGLIVTEGVPISP
CCCCCCHHHHHHHHHHCCEEEECHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
SAEGFLFIPGLFRPDQIIGWRKVTDAVHAEGGTIFAQLWHVGRVSHVSNQPGGIAPVSST
CCCCEEEECCCCCCCHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCC
EHPALHSEAWGLRDDGTPGAVPVSTPRALSTAEVAGVIEDFAKAAANAIAAGFDGVELHG
CCCCCCHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLIEQFLNPNVNDRTDAYRGDTLEGRTRFVIEAIDAVIARIGAERTAIRLSPYGGLF
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DMQPYPQIEETYLFLADAFSRRGLAYVHLMDQKSRGSTAIPQNFLAEFRTCYKGALILAG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEC
GLTRDRAETLIAEGMIDLAAFGEPFIANPDLVARLEKGLPLALADRSLHYGGGAHGYTDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEECCCEECCCCCCCCCCE
SVYHGEGVRL
EEECCCCCCC
>Mature Secondary Structure 
GRLFEPFRLGKYLLRNRVVMAPMTRARNPDGVPNDLNALYYRQRASAGLIVTEGVPISP
CCCCCHHHHHHHHHHCCEEEECHHHCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
SAEGFLFIPGLFRPDQIIGWRKVTDAVHAEGGTIFAQLWHVGRVSHVSNQPGGIAPVSST
CCCCEEEECCCCCCCHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCC
EHPALHSEAWGLRDDGTPGAVPVSTPRALSTAEVAGVIEDFAKAAANAIAAGFDGVELHG
CCCCCCHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
ANGYLIEQFLNPNVNDRTDAYRGDTLEGRTRFVIEAIDAVIARIGAERTAIRLSPYGGLF
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
DMQPYPQIEETYLFLADAFSRRGLAYVHLMDQKSRGSTAIPQNFLAEFRTCYKGALILAG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEC
GLTRDRAETLIAEGMIDLAAFGEPFIANPDLVARLEKGLPLALADRSLHYGGGAHGYTDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEECCCEECCCCCCCCCCE
SVYHGEGVRL
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]