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The map label for this gene is thiC [H]

Identifier: 182677065

GI number: 182677065

Start: 65496

End: 67340

Strand: Direct

Name: thiC [H]

Synonym: Bind_0063

Alternate gene names: 182677065

Gene position: 65496-67340 (Clockwise)

Preceding gene: 182677064

Following gene: 182677066

Centisome position: 1.57

GC content: 60.76

Gene sequence:

>1845_bases
ATGAACATTCAGCACAAACGCTCAAGTCTCGTTCCCGAGACTGTGACCTGCGGCCCTCTGCCGGGATCGCGCAAGGTCTA
TCACCATCCTCAGAGCCATCCCGAACTCAGCGTGCCGTTCCGCGAGATCGCGCTCGATCCGGCGTCTGGCGAGCCGCCGG
TGCGCGTCTATGATGCCTCTGGTCCCTATTCGGAAGAGAATTTCAAGCCCGATCTCGCCAAGGGCCTGCCGCGCACCCGC
GCCGTCTGGCTCGAAAAACGCGCGGGCCATGAGTCCTATGCCGGACGTGCCGTCAAGCCGGAAGACAATGGCTCCGTTTC
GGCCGACCGCTTGGTGCCGCCCTGCCCGGCCAATGCCGCGCCCTTGCGCGGGCGCACCGGTGCCTTGGTGACGCAATATG
AATTCGCCAAGGCTGGCATCATTACGGAAGAAATGATCTACGCCGCCGCGCGCGAAAATCTCGGCCGCGAACAGGCCGTG
GAGGGAGCCGCCGCCCGGCTCAAGGATGGCGAAAGCTTTGGCGCCTCGATCCCCGAATTCGTGACACCGGAATTCGTGCG
CGACGAGATCGCGCGGGGCCGTGCGATCATTCCCGCCAATATCAATCATCCCGAACTCGAGCCGATGGTCATCGGCCGCA
ATTTTCTCGTGAAGGTCAATGCCAATATCGGCAATTCGGCTGTGACCTCTTCCGTTGCGGAAGAAGTCGAAAAGCTCGTC
TGGGCGATCCGCTGGGGCGCCGACACGGTCATGGACCTTTCGACCGGCCGCAACATTCACAATATCCGCGACTGGATCAT
GCGCAATGCGCCGGTGCCGATCGGCACCGTGCCGATCTATCAGGCGCTCGAAAAGGTCGACGGCGATCCGATCAAACTGA
CCTGGGAAATTTTCCGCGACACTTTGATCGAACAAGCCGAGCAGGGCGTCGATTATTTCACGATCCATGCAGGTGTCCGG
CTGGCCCATGTGCCGCTGACCGCCAAGCGCACCACGGGCATCGTCTCGCGCGGTGGCTCGATCATGGCGCGCTGGTGCCT
CGCGCATCACAAGGAAAGCTTCCTCTACGAGCATTTCGACGAGATTTGCGACATCATGCGCGCCTATGATGTCTCGTTCT
CGCTGGGCGATGGCCTGCGGCCCGGCTCGATTGCCGATGCCAATGATGCGGCGCAATTCGCCGAACTCGAAACGCTGGGC
GAATTGACCAAGATCGCCTGGGCCAAAGGCTGTCAGGTCATGATCGAAGGCCCCGGCCATGTGCCGATCCATAAGATCAA
GATCAATATGGAAAAGCAGCTCAAGGAATGCGATGAGGCGCCCTTCTATACGCTTGGGCCGCTAACGACCGATATTGCGC
CTGGCTATGATCACATCACCTCCGGCATTGGTGCGGCGATGATCGGCTGGTTCGGCTGCGCCATGCTCTGCTATGTGACG
CCGAAGGAGCATTTGGGTCTGCCCGACCGCGACGATGTGAAAGTGGGCGTGATCACCTATCGCATCGCGGCCCATGCGGG
CGATCTCGCCAAGGGCCATCCGGCGGCTCAGATCCGCGATGATGCCGTCTCGCGGGCGCGGTTCGATTTCCGCTGGCAGG
ATCAGTTCAATCTCGGCCTCGATCCGGAAACGGCGGCGCATTTCCACGATGAGACCCTGCCGAAGGACGCCCATAAGGTC
GCCCATTTCTGCTCCATGTGCGGACCGCAATTCTGCTCGATGAAGATCACCCAGGATCTGCGTGTGGAAGCCGCCGCCAT
GGCGGAAGAGGAAGCCAAGCGCGAACAGGGCATGGCCGAAAAGAGTGCCGAATTTTTGGAGAAGGGCGGCAAGCTTTACG
TATAA

Upstream 100 bases:

>100_bases
CATTCCGATCCGCCCGCCCGCCTCTGCCGACATCATCGATAAGCCTCGCTCTCGAGCCGAACGCGGAGCGAGCCGAAGAG
TGAGTGGAGGAAACCGGCTC

Downstream 100 bases:

>100_bases
CCATCCGCGCTTTAATGTGGAATGGTCCGGGGCATATTCAGCGCCCGGATGTCGCGGCCGCGATATCATCAAGCTTTGAA
TAGGGGAGCCGGTGTACGTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MNIQHKRSSLVPETVTCGPLPGSRKVYHHPQSHPELSVPFREIALDPASGEPPVRVYDASGPYSEENFKPDLAKGLPRTR
AVWLEKRAGHESYAGRAVKPEDNGSVSADRLVPPCPANAAPLRGRTGALVTQYEFAKAGIITEEMIYAAARENLGREQAV
EGAAARLKDGESFGASIPEFVTPEFVRDEIARGRAIIPANINHPELEPMVIGRNFLVKVNANIGNSAVTSSVAEEVEKLV
WAIRWGADTVMDLSTGRNIHNIRDWIMRNAPVPIGTVPIYQALEKVDGDPIKLTWEIFRDTLIEQAEQGVDYFTIHAGVR
LAHVPLTAKRTTGIVSRGGSIMARWCLAHHKESFLYEHFDEICDIMRAYDVSFSLGDGLRPGSIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPIHKIKINMEKQLKECDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVT
PKEHLGLPDRDDVKVGVITYRIAAHAGDLAKGHPAAQIRDDAVSRARFDFRWQDQFNLGLDPETAAHFHDETLPKDAHKV
AHFCSMCGPQFCSMKITQDLRVEAAAMAEEEAKREQGMAEKSAEFLEKGGKLYV

Sequences:

>Translated_614_residues
MNIQHKRSSLVPETVTCGPLPGSRKVYHHPQSHPELSVPFREIALDPASGEPPVRVYDASGPYSEENFKPDLAKGLPRTR
AVWLEKRAGHESYAGRAVKPEDNGSVSADRLVPPCPANAAPLRGRTGALVTQYEFAKAGIITEEMIYAAARENLGREQAV
EGAAARLKDGESFGASIPEFVTPEFVRDEIARGRAIIPANINHPELEPMVIGRNFLVKVNANIGNSAVTSSVAEEVEKLV
WAIRWGADTVMDLSTGRNIHNIRDWIMRNAPVPIGTVPIYQALEKVDGDPIKLTWEIFRDTLIEQAEQGVDYFTIHAGVR
LAHVPLTAKRTTGIVSRGGSIMARWCLAHHKESFLYEHFDEICDIMRAYDVSFSLGDGLRPGSIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPIHKIKINMEKQLKECDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVT
PKEHLGLPDRDDVKVGVITYRIAAHAGDLAKGHPAAQIRDDAVSRARFDFRWQDQFNLGLDPETAAHFHDETLPKDAHKV
AHFCSMCGPQFCSMKITQDLRVEAAAMAEEEAKREQGMAEKSAEFLEKGGKLYV
>Mature_614_residues
MNIQHKRSSLVPETVTCGPLPGSRKVYHHPQSHPELSVPFREIALDPASGEPPVRVYDASGPYSEENFKPDLAKGLPRTR
AVWLEKRAGHESYAGRAVKPEDNGSVSADRLVPPCPANAAPLRGRTGALVTQYEFAKAGIITEEMIYAAARENLGREQAV
EGAAARLKDGESFGASIPEFVTPEFVRDEIARGRAIIPANINHPELEPMVIGRNFLVKVNANIGNSAVTSSVAEEVEKLV
WAIRWGADTVMDLSTGRNIHNIRDWIMRNAPVPIGTVPIYQALEKVDGDPIKLTWEIFRDTLIEQAEQGVDYFTIHAGVR
LAHVPLTAKRTTGIVSRGGSIMARWCLAHHKESFLYEHFDEICDIMRAYDVSFSLGDGLRPGSIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPIHKIKINMEKQLKECDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVT
PKEHLGLPDRDDVKVGVITYRIAAHAGDLAKGHPAAQIRDDAVSRARFDFRWQDQFNLGLDPETAAHFHDETLPKDAHKV
AHFCSMCGPQFCSMKITQDLRVEAAAMAEEEAKREQGMAEKSAEFLEKGGKLYV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=627, Percent_Identity=62.5199362041467, Blast_Score=786, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 67498; Mature: 67498

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQHKRSSLVPETVTCGPLPGSRKVYHHPQSHPELSVPFREIALDPASGEPPVRVYDAS
CCCCCCHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEEECC
GPYSEENFKPDLAKGLPRTRAVWLEKRAGHESYAGRAVKPEDNGSVSADRLVPPCPANAA
CCCCCCCCCCHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLRGRTGALVTQYEFAKAGIITEEMIYAAARENLGREQAVEGAAARLKDGESFGASIPEF
CCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCC
VTPEFVRDEIARGRAIIPANINHPELEPMVIGRNFLVKVNANIGNSAVTSSVAEEVEKLV
CCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
WAIRWGADTVMDLSTGRNIHNIRDWIMRNAPVPIGTVPIYQALEKVDGDPIKLTWEIFRD
HHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHH
TLIEQAEQGVDYFTIHAGVRLAHVPLTAKRTTGIVSRGGSIMARWCLAHHKESFLYEHFD
HHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EICDIMRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGH
HHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
VPIHKIKINMEKQLKECDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVT
CCEEEEEECHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEC
PKEHLGLPDRDDVKVGVITYRIAAHAGDLAKGHPAAQIRDDAVSRARFDFRWQDQFNLGL
CHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCC
DPETAAHFHDETLPKDAHKVAHFCSMCGPQFCSMKITQDLRVEAAAMAEEEAKREQGMAE
CCCHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCEECCHHHHHHHHHHHHHHHHHHHCCHH
KSAEFLEKGGKLYV
HHHHHHHCCCCCCC
>Mature Secondary Structure
MNIQHKRSSLVPETVTCGPLPGSRKVYHHPQSHPELSVPFREIALDPASGEPPVRVYDAS
CCCCCCHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEEECC
GPYSEENFKPDLAKGLPRTRAVWLEKRAGHESYAGRAVKPEDNGSVSADRLVPPCPANAA
CCCCCCCCCCHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLRGRTGALVTQYEFAKAGIITEEMIYAAARENLGREQAVEGAAARLKDGESFGASIPEF
CCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCC
VTPEFVRDEIARGRAIIPANINHPELEPMVIGRNFLVKVNANIGNSAVTSSVAEEVEKLV
CCHHHHHHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
WAIRWGADTVMDLSTGRNIHNIRDWIMRNAPVPIGTVPIYQALEKVDGDPIKLTWEIFRD
HHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHH
TLIEQAEQGVDYFTIHAGVRLAHVPLTAKRTTGIVSRGGSIMARWCLAHHKESFLYEHFD
HHHHHHHCCCCEEEEECCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EICDIMRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGH
HHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
VPIHKIKINMEKQLKECDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVT
CCEEEEEECHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEC
PKEHLGLPDRDDVKVGVITYRIAAHAGDLAKGHPAAQIRDDAVSRARFDFRWQDQFNLGL
CHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCC
DPETAAHFHDETLPKDAHKVAHFCSMCGPQFCSMKITQDLRVEAAAMAEEEAKREQGMAE
CCCHHHHHCCCCCCHHHHHHHHHHHHCCHHHHCEECCHHHHHHHHHHHHHHHHHHHCCHH
KSAEFLEKGGKLYV
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]