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The map label for this gene is rep [H]

Identifier: 182682653

GI number: 182682653

Start: 2416303

End: 2418279

Strand: Reverse

Name: rep [H]

Synonym: XfasM23_2143

Alternate gene names: 182682653

Gene position: 2418279-2416303 (Counterclockwise)

Preceding gene: 182682654

Following gene: 182682652

Centisome position: 95.37

GC content: 50.89

Gene sequence:

>1977_bases
ATGCACGGCCTCAATTCTCCTCAACGTGCTGCGGTCTTATACCGTGAAGGTCCTCTGCTGGTGCTTGCGGGTGCTGGCAG
CGGCAAGACGCGTGTGATTGTGGAGAAGATTGCGTACTTGATCACCTCAGATTGTTATCCTGCTAAACGTATTGCAGCGA
TTACATTTACCAACAAGTCCGCGAAGGAAATGCGTGAACGTGTGGCGAAGCAGATCAAAAGTAGTCGTGCTGATGGATTG
ACGATCTGTACCTTTCATGCATTGGGACTGAAGTTTTTGCAGATTGAGCATGAGGCCGTTGGACTGAAGCCTGGCTTTTC
GGTGTTCGATGCAGATGATGCTGATGCCCAGATCAATGACCTGTTGCATGGAGCCAAACAGGATGTGATTGATGATACGA
AAAGACGGATATCCCGTGCTAAGAATGCTGGTTTGTCACCTGAACAAGCACTTGCTGCTGCACGTACCACGCGTGACATG
GAGGTGGCAACGTTATATGCACGCTATCAAGCACGCCTGATAGCGTTCAACGCCGTTGATTTTGATGACTTGATTCGCCT
GCCGGTACAGATCCTTGAAACGAGTGATCAGGTTGCGATTGCTTGGCGTGAACGGATTGGTTACTTGTTGGTCGACGAGT
GTCAGGATACCAACGATGCACAATATCGATTACTAAAGGTGCTGGCCAGTCCGCGTGGCAACTTCACCTGTGTAGGGGAT
GATGATCAGAGTATCTATGCTTGGCGAGGTGCTAATGCTGAGAACTTAATGCAACTGGAGAGAGATTACCCACAATTGAA
AGTCATCAAGTTGGAACAGAACTACCGTTGTCCTAACCGTGTGCTGCGTGCTGCCAATGCGTTGATTGCACATAACCCAC
ATGCGCATCTGAAGACGTTGTGGAGTGACCAGAAAGACGGTGAGCGCATCCGTATTTGGGAGTGCCGAGACAGCGAACAT
GAGGCTGAAAAGATCGCAGCGGAAATCTCTTATTTGGGGGATGCCAAGCAGGTACCGTGGAGTGATTTCTGCATCTTGTT
CCGAGGTAACTTTCAATCGCGTCCGCTGGAAAAGGCACTGCAATTATTGCGTATCCCTTACCACTTGACGGGTGGTACCG
CGTTTCTGGAACGTCAAGAAGTGAAGAACCTGTTGTCTTGGCTACGATTGTTGGTTAACCCTGATGACGACGCCGCATTT
CTCCGTGCAGTGCAGTCACCCAAGCGTGAGGTTGGTTCCACCTCATTGGCTAAATTGGCTGAACTTGCGGCGACAAAGCA
AATGCCGATGTCGCGTGCTGCTGAGTCGCTGGGCGCGTTGCAACAATTACCGGCGCGCGCAGCCAGTGGCCTGAATAAAT
TCACTGATATCTTGCGTGAGTTGCGCCAGCAAGTATCACGTGTGTCGGCGGCTGAGTTAGTGCGGCGCCTTGCTGAAGTT
TCTGGTTTACTTACCGAATTGCGTGAGCAGTCCAATGATGAAGCGAGTTACCAACGGCGTAAGCGTAATGTAGAGGAACT
GGCCGAATGGTTTGAAGGCGACCCTTTCGGTGCCAGTGTTGGCGATCTTGTTTCTCAGCTAGCATTGCTATCACGAAATG
ATAAGGACGATGGTGGTAACCAGGTGCGCATGATGACACTGCACGCTTCCAAAGGATTGGAGTTTCGTTATGTGTTCATC
GCTGGTTGCGAAGATGGCGTGCTACCGCATGAAGTCAGCTTGGAAGAAGGCAATGTGCAAGAGGAGCGGCGCCTATTATA
TGTTGGTATCACCCGAGCCAAAGAGCAGTTGTGGATGAGCTATAGCAAATGTGCGCGCCGCTTCGGTGAACGTGTGCGGT
TTAAGCCCAGTCGCTTTTTCGATGAACTGCCAGCTGAGGAATTACAGTGCGATGGTGCTGATCCTGCGGTTGATGCCGAA
CGTAAACAGGAGCGTGCTAAGGCTGGTTTGGCAGCAATTCAGGCGCTATTGGACTGA

Upstream 100 bases:

>100_bases
ATCCCTTGCTCGATTAGTAATCAATACCGATGCCGTGTCACAAGCAAATGGGCTTCCATATACTGATCGGTACAATACGT
ATCCGGAAAACGTTTTCCTT

Downstream 100 bases:

>100_bases
GTGATTCCGTGTATTCGACTTTAGCTGGCCTGGACAGATTTAGATGTCACTTGAGCTGCAAGCAGAATGCAATAGTGATT
GTTCGCTGTGGTATCTATTC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MHGLNSPQRAAVLYREGPLLVLAGAGSGKTRVIVEKIAYLITSDCYPAKRIAAITFTNKSAKEMRERVAKQIKSSRADGL
TICTFHALGLKFLQIEHEAVGLKPGFSVFDADDADAQINDLLHGAKQDVIDDTKRRISRAKNAGLSPEQALAAARTTRDM
EVATLYARYQARLIAFNAVDFDDLIRLPVQILETSDQVAIAWRERIGYLLVDECQDTNDAQYRLLKVLASPRGNFTCVGD
DDQSIYAWRGANAENLMQLERDYPQLKVIKLEQNYRCPNRVLRAANALIAHNPHAHLKTLWSDQKDGERIRIWECRDSEH
EAEKIAAEISYLGDAKQVPWSDFCILFRGNFQSRPLEKALQLLRIPYHLTGGTAFLERQEVKNLLSWLRLLVNPDDDAAF
LRAVQSPKREVGSTSLAKLAELAATKQMPMSRAAESLGALQQLPARAASGLNKFTDILRELRQQVSRVSAAELVRRLAEV
SGLLTELREQSNDEASYQRRKRNVEELAEWFEGDPFGASVGDLVSQLALLSRNDKDDGGNQVRMMTLHASKGLEFRYVFI
AGCEDGVLPHEVSLEEGNVQEERRLLYVGITRAKEQLWMSYSKCARRFGERVRFKPSRFFDELPAEELQCDGADPAVDAE
RKQERAKAGLAAIQALLD

Sequences:

>Translated_658_residues
MHGLNSPQRAAVLYREGPLLVLAGAGSGKTRVIVEKIAYLITSDCYPAKRIAAITFTNKSAKEMRERVAKQIKSSRADGL
TICTFHALGLKFLQIEHEAVGLKPGFSVFDADDADAQINDLLHGAKQDVIDDTKRRISRAKNAGLSPEQALAAARTTRDM
EVATLYARYQARLIAFNAVDFDDLIRLPVQILETSDQVAIAWRERIGYLLVDECQDTNDAQYRLLKVLASPRGNFTCVGD
DDQSIYAWRGANAENLMQLERDYPQLKVIKLEQNYRCPNRVLRAANALIAHNPHAHLKTLWSDQKDGERIRIWECRDSEH
EAEKIAAEISYLGDAKQVPWSDFCILFRGNFQSRPLEKALQLLRIPYHLTGGTAFLERQEVKNLLSWLRLLVNPDDDAAF
LRAVQSPKREVGSTSLAKLAELAATKQMPMSRAAESLGALQQLPARAASGLNKFTDILRELRQQVSRVSAAELVRRLAEV
SGLLTELREQSNDEASYQRRKRNVEELAEWFEGDPFGASVGDLVSQLALLSRNDKDDGGNQVRMMTLHASKGLEFRYVFI
AGCEDGVLPHEVSLEEGNVQEERRLLYVGITRAKEQLWMSYSKCARRFGERVRFKPSRFFDELPAEELQCDGADPAVDAE
RKQERAKAGLAAIQALLD
>Mature_658_residues
MHGLNSPQRAAVLYREGPLLVLAGAGSGKTRVIVEKIAYLITSDCYPAKRIAAITFTNKSAKEMRERVAKQIKSSRADGL
TICTFHALGLKFLQIEHEAVGLKPGFSVFDADDADAQINDLLHGAKQDVIDDTKRRISRAKNAGLSPEQALAAARTTRDM
EVATLYARYQARLIAFNAVDFDDLIRLPVQILETSDQVAIAWRERIGYLLVDECQDTNDAQYRLLKVLASPRGNFTCVGD
DDQSIYAWRGANAENLMQLERDYPQLKVIKLEQNYRCPNRVLRAANALIAHNPHAHLKTLWSDQKDGERIRIWECRDSEH
EAEKIAAEISYLGDAKQVPWSDFCILFRGNFQSRPLEKALQLLRIPYHLTGGTAFLERQEVKNLLSWLRLLVNPDDDAAF
LRAVQSPKREVGSTSLAKLAELAATKQMPMSRAAESLGALQQLPARAASGLNKFTDILRELRQQVSRVSAAELVRRLAEV
SGLLTELREQSNDEASYQRRKRNVEELAEWFEGDPFGASVGDLVSQLALLSRNDKDDGGNQVRMMTLHASKGLEFRYVFI
AGCEDGVLPHEVSLEEGNVQEERRLLYVGITRAKEQLWMSYSKCARRFGERVRFKPSRFFDELPAEELQCDGADPAVDAE
RKQERAKAGLAAIQALLD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=666, Percent_Identity=44.7447447447448, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI2367296, Length=636, Percent_Identity=37.8930817610063, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787196, Length=335, Percent_Identity=28.955223880597, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=731, Percent_Identity=27.0861833105335, Blast_Score=206, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73851; Mature: 73851

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGLNSPQRAAVLYREGPLLVLAGAGSGKTRVIVEKIAYLITSDCYPAKRIAAITFTNKS
CCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHEEEEEECCCH
AKEMRERVAKQIKSSRADGLTICTFHALGLKFLQIEHEAVGLKPGFSVFDADDADAQIND
HHHHHHHHHHHHHHCCCCCEEEEHHHHHCHHHEEEEHHHCCCCCCCCEECCCCCCHHHHH
LLHGAKQDVIDDTKRRISRAKNAGLSPEQALAAARTTRDMEVATLYARYQARLIAFNAVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCC
FDDLIRLPVQILETSDQVAIAWRERIGYLLVDECQDTNDAQYRLLKVLASPRGNFTCVGD
HHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANAENLMQLERDYPQLKVIKLEQNYRCPNRVLRAANALIAHNPHAHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHH
WSDQKDGERIRIWECRDSEHEAEKIAAEISYLGDAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRIPYHLTGGTAFLERQEVKNLLSWLRLLVNPDDDAAFLRAVQSPKREVGSTSLAKLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ELAATKQMPMSRAAESLGALQQLPARAASGLNKFTDILRELRQQVSRVSAAELVRRLAEV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLLTELREQSNDEASYQRRKRNVEELAEWFEGDPFGASVGDLVSQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTLHASKGLEFRYVFIAGCEDGVLPHEVSLEEGNVQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
YSKCARRFGERVRFKPSRFFDELPAEELQCDGADPAVDAERKQERAKAGLAAIQALLD
HHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHGLNSPQRAAVLYREGPLLVLAGAGSGKTRVIVEKIAYLITSDCYPAKRIAAITFTNKS
CCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHEEEEEECCCH
AKEMRERVAKQIKSSRADGLTICTFHALGLKFLQIEHEAVGLKPGFSVFDADDADAQIND
HHHHHHHHHHHHHHCCCCCEEEEHHHHHCHHHEEEEHHHCCCCCCCCEECCCCCCHHHHH
LLHGAKQDVIDDTKRRISRAKNAGLSPEQALAAARTTRDMEVATLYARYQARLIAFNAVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCC
FDDLIRLPVQILETSDQVAIAWRERIGYLLVDECQDTNDAQYRLLKVLASPRGNFTCVGD
HHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANAENLMQLERDYPQLKVIKLEQNYRCPNRVLRAANALIAHNPHAHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHH
WSDQKDGERIRIWECRDSEHEAEKIAAEISYLGDAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRIPYHLTGGTAFLERQEVKNLLSWLRLLVNPDDDAAFLRAVQSPKREVGSTSLAKLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ELAATKQMPMSRAAESLGALQQLPARAASGLNKFTDILRELRQQVSRVSAAELVRRLAEV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLLTELREQSNDEASYQRRKRNVEELAEWFEGDPFGASVGDLVSQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTLHASKGLEFRYVFIAGCEDGVLPHEVSLEEGNVQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
YSKCARRFGERVRFKPSRFFDELPAEELQCDGADPAVDAERKQERAKAGLAAIQALLD
HHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]