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The map label for this gene is prfA

Identifier: 182682635

GI number: 182682635

Start: 2394315

End: 2395400

Strand: Direct

Name: prfA

Synonym: XfasM23_2123

Alternate gene names: 182682635

Gene position: 2394315-2395400 (Clockwise)

Preceding gene: 182682634

Following gene: 182682639

Centisome position: 94.42

GC content: 56.81

Gene sequence:

>1086_bases
ATGAAACCGACCCTGCGTCGTAAACTGGAAGCACTCGCCGAGCGCCATGAAGAACTCGAACGCTTGCTCTCTGATCCCAA
AATCGCCTCTGACACCGACAGATTCCGTACATACTCCCGCGAATTGGCGCAACTCGCACCGATTGCGACCACACTGGCCG
AAGAAACCCGTACCAAAGCCGATCTAGCCGCCGCTGAAACACTACGCACCGACCCTGAAATGCGCGAACTTGCCGAACAA
GAAATCGCCATAGCCCAAGCACACCTGACCACACTTGACGAACAACTTCAGCGACTCCTGATTCCTCAAGACCCCCGAGA
CGAATGCAACCTATTCCTGGAAGTACGCGCCGGCACTGGTGGCGACGAAGCTGCAATCTTTGCCGGCAACCTCTTCCGGA
TGTACACCCGCTACGCCGAACGCCAGCGCTGGAAAGTCGAAGTGGAATCAGATACTCCCGGCGAACATGGCGGCTATAAA
GAAATCATAGCGCGCATTGTCGGACGCGGCGCCTACTCCCGACTTAAATTTGAATCCGGCACACACCGCGTACAACGCGT
GCCCGCAACCGAATCCCAGGGACGCATCCATACTTCAGCAGCCACCGTCGCGATTATTCCAGAAGCCGACGAAATCGCAG
ACATCTCGATCAACCCCGCTGACCTCAAGATCGACACATTCCGCTCCTCCGGTGCCGGCGGCCAACACGTCAACAAAACC
GAATCGGCAATCCGCATCACCCATCTCCCAACCGGCGTCGTCGTCGAATCCCAAACCGAACGCAGCCAACACGCCAACCG
CGACAAAGCAATGAAACGGCTCAAAGCACAACTGATCGAAAGCCAACGCAGCCAACAGGCCACAGCCGAAGCAATGACAC
GTAAACTGCAAGTCGGCAGCGGCGACCGCAGCCAACGTATCCGCACCTACAACTTTCCCCAAGGCCGGATCACCGATCAC
CGTGTCGAAAACCTGACACTCTACGACCTTCCCAACATCATCGAAGGCGACCTAGACCCCCTGATCGACCGACTACGACA
AGAACACCAAGCCGAAGAACTCGCACGACTGAGCAACGCCCCATAA

Upstream 100 bases:

>100_bases
CTCGCCAACAACACCGAGCTAGTTCGTGCAGCAGAACAACTCTTTCCAGAGAAACCCGGCTACCACCACCCCACCCTTCA
GACAACGATTGTGAAAACCG

Downstream 100 bases:

>100_bases
CACCAGCCTGAAACCTCACTGGAACTTCATTCAACAACCTTGCCTAACAACACCACCATGCACAGACACTGACTGAACAG
AAACCGGAGATAACCCCACA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKPTLRRKLEALAERHEELERLLSDPKIASDTDRFRTYSRELAQLAPIATTLAEETRTKADLAAAETLRTDPEMRELAEQ
EIAIAQAHLTTLDEQLQRLLIPQDPRDECNLFLEVRAGTGGDEAAIFAGNLFRMYTRYAERQRWKVEVESDTPGEHGGYK
EIIARIVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADEIADISINPADLKIDTFRSSGAGGQHVNKT
ESAIRITHLPTGVVVESQTERSQHANRDKAMKRLKAQLIESQRSQQATAEAMTRKLQVGSGDRSQRIRTYNFPQGRITDH
RVENLTLYDLPNIIEGDLDPLIDRLRQEHQAEELARLSNAP

Sequences:

>Translated_361_residues
MKPTLRRKLEALAERHEELERLLSDPKIASDTDRFRTYSRELAQLAPIATTLAEETRTKADLAAAETLRTDPEMRELAEQ
EIAIAQAHLTTLDEQLQRLLIPQDPRDECNLFLEVRAGTGGDEAAIFAGNLFRMYTRYAERQRWKVEVESDTPGEHGGYK
EIIARIVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADEIADISINPADLKIDTFRSSGAGGQHVNKT
ESAIRITHLPTGVVVESQTERSQHANRDKAMKRLKAQLIESQRSQQATAEAMTRKLQVGSGDRSQRIRTYNFPQGRITDH
RVENLTLYDLPNIIEGDLDPLIDRLRQEHQAEELARLSNAP
>Mature_361_residues
MKPTLRRKLEALAERHEELERLLSDPKIASDTDRFRTYSRELAQLAPIATTLAEETRTKADLAAAETLRTDPEMRELAEQ
EIAIAQAHLTTLDEQLQRLLIPQDPRDECNLFLEVRAGTGGDEAAIFAGNLFRMYTRYAERQRWKVEVESDTPGEHGGYK
EIIARIVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADEIADISINPADLKIDTFRSSGAGGQHVNKT
ESAIRITHLPTGVVVESQTERSQHANRDKAMKRLKAQLIESQRSQQATAEAMTRKLQVGSGDRSQRIRTYNFPQGRITDH
RVENLTLYDLPNIIEGDLDPLIDRLRQEHQAEELARLSNAP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=49.8269896193772, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI34577120, Length=370, Percent_Identity=39.1891891891892, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=53.6312849162011, Blast_Score=194, Evalue=8e-50,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.1731843575419, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=37.1681415929204, Blast_Score=212, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=318, Percent_Identity=34.2767295597484, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=362, Percent_Identity=39.7790055248619, Blast_Score=236, Evalue=5e-63,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=42.2018348623853, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_XYLF2 (B2IA85)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830795.1
- ProteinModelPortal:   B2IA85
- SMR:   B2IA85
- GeneID:   6204139
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2123
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40613; Mature: 40613

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLRRKLEALAERHEELERLLSDPKIASDTDRFRTYSRELAQLAPIATTLAEETRTKA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAAETLRTDPEMRELAEQEIAIAQAHLTTLDEQLQRLLIPQDPRDECNLFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
GDEAAIFAGNLFRMYTRYAERQRWKVEVESDTPGEHGGYKEIIARIVGRGAYSRLKFESG
CCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADEIADISINPADLKIDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCC
ESAIRITHLPTGVVVESQTERSQHANRDKAMKRLKAQLIESQRSQQATAEAMTRKLQVGS
CCEEEEEECCCCCEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSQRIRTYNFPQGRITDHRVENLTLYDLPNIIEGDLDPLIDRLRQEHQAEELARLSNA
CCCCCCCEEECCCCCCCCCHHHCEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MKPTLRRKLEALAERHEELERLLSDPKIASDTDRFRTYSRELAQLAPIATTLAEETRTKA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAAETLRTDPEMRELAEQEIAIAQAHLTTLDEQLQRLLIPQDPRDECNLFLEVRAGTG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
GDEAAIFAGNLFRMYTRYAERQRWKVEVESDTPGEHGGYKEIIARIVGRGAYSRLKFESG
CCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADEIADISINPADLKIDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCC
ESAIRITHLPTGVVVESQTERSQHANRDKAMKRLKAQLIESQRSQQATAEAMTRKLQVGS
CCEEEEEECCCCCEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSQRIRTYNFPQGRITDHRVENLTLYDLPNIIEGDLDPLIDRLRQEHQAEELARLSNA
CCCCCCCEEECCCCCCCCCHHHCEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA