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The map label for this gene is dosP [H]

Identifier: 182682611

GI number: 182682611

Start: 2362146

End: 2364218

Strand: Direct

Name: dosP [H]

Synonym: XfasM23_2099

Alternate gene names: 182682611

Gene position: 2362146-2364218 (Clockwise)

Preceding gene: 182682606

Following gene: 182682634

Centisome position: 93.16

GC content: 47.61

Gene sequence:

>2073_bases
ATGAAAAAAAACAAAGAACGCACGCTAAGACTGATGATCATTGACGATAACCCTGAGAATACCGAAGCGATCGTTACTTT
GCTGCGAAATAGCGGCATTGCAGTGCGACCATCCCGGCCACAGCACCTAGAAGACGTGACAATGATGCTCAGTCACCCAA
TTGACCTAGTACTAGTCGGCAATTCCTCCCAAATATCACTCAGAGCACTACATCAACAAATTTCCACTAGTGGCAAAGAC
GTCCCCCTGATCCAACTGACTTGGCAACTCAACGAGAAAGAGTGGATTGCTGCAACCGGTTTCGGTATCCGCTCAATCGC
CTTAGGTCACCACCCCAAACACCTACTGGAAGTGGTAAACAACGAAATTGAAGCGCTGCAAATACGCCGCAGCCTACGCC
GATTCGAAGCGCAAATACGGGAGACCGAACGCCGCTGTAATGCGCTGATCGCATCGTCAAGAGATCCAATCGCCTATGTG
CATGAAGGCATGCACATTCAGGCAAACCAAGCTTATCTGGAAATGTTTGGATTCGAATCGTTTGAAGACATTGAGGGGCT
ACCACTGTTAGATTTGATTGCAGCTCCACACATGGAAGGATTCAAGCATCTGCTCAAAACACTCTCCAAAGGAGAGAAAC
CACCATCGCAATATAAATTAGATGCGCGTCACGTAAATGGCAACATATTTCCAGCAACAATGGAGTTCACCACCACAACA
TACGAAGGTGAACCCTGCATCCAGGTAGTGCTCCGTGGCTACGAAGAATTCGACCAAGAATTGACACGTGAAATCGAAAA
ACTGCGTCAACGTGATCCTATTACCGGGCTTCTAAATCGCCAAGCCTTCATCATCGCATTAGAAGATGCTGTAGCACGCG
CCGGTATGAACGGAGATCAATTTGGCTTGCTACTAGTTGAACCTAACCATTACACAAGAATCCTACAAGAAATCGGCTTG
GACTCGGCGGACACACTCATCACCTCACTCGCAAATTTCTTTTCTGAAATGATCGATTCAAAAGTAGCAGTGCGAACCGC
GCGTTTCTGCGAGACCAGATTCGCACTACTGTTAGAGGGCGATTACACCCATACCATAACATTGGCTGAACGTATCCGCA
TTGACATCGCACAACATATATTCCTGCTTGGAAAACACTCAACCACAGTCACAGTAAGCATTGGCGGTGTACAAATAGGC
GAACGCATCGCCAATTTGTGCAAAGTACTCCATCACGCCGCCGAGTCAGTTCAGATTGCCACTCAGCTTGGTGGCAACAC
TGCCATAATCTACGATCCAGCGGCAGCGGATCGCGCAGAGGAACTGCGAATCCAGAACTTGCTAAAACAACTGCAGCAAG
CAGTGACAAACAACGATTTGACCATTTATTACCAACCGATAATGAGTCTACAGCAACAATCCCTAGAGCTATACCAAATT
TACATAGGCCTGCATGGAGATGAAGAACTCATTTCGTCGGTGACACTGCTAGAAATCGCCAAGAAACGCGAGTTGATCGC
ACAAATTGATCGATGGGTAGTTGCGCGTGCGATCGCACAGATCGGTGAACGCCAGCGGGCCGGCCATACCACGCATTTAC
TGGTACAGATCGACTCGGCTTCGTTTACCGACCCGCAGTTACTCACCATGCTACGTACAGAATTAGCAGCACATCATGTT
CGTGGGGAGCAACTGTGGTTACAAACGCCGGAATCAAAAGTGTTCACCCATTTATATAATGCGCAGCAGTTTCTTAGTGA
AATCTCAGTACAAGGCTGCAAAATGGGGCTAGAACAGTTCGGTACAGGGCTGGACTCATTTCAGTTATTGACACATTTCA
AGCCAGCATTCCTAAAATTGGATCGTAACCTAACTCAAGACATCAACTCCAACCATGACAGTCTAGAAAAGATTCGTGAG
ATAACTTCACGTGCGCAAGACAACGGAATTGCAACCTTCGCCGAATTCGTGACCGACTCAACGTCGATGAGCGCATTATT
CAAGGTAGGCGTGGACTACGTGCAAGGAGACTTCGTCGCGCCAATCAGCGCAACAATGAACAACGAGTTCTAA

Upstream 100 bases:

>100_bases
TCCATATCTGCATTTCTCGATCAAAAGGATAAAGCACCTAAGCGGCCATGCAGTGCATGCATAGATTGCTCGTGTCCCCT
CTCATCCCTTGGACTAACGC

Downstream 100 bases:

>100_bases
TACGACTTAGTGTTACCACCTCAACAAAGTTGGAAAAGATACATAAAAATAGTCCTTTATTTTCTTTCCGAACCATAGTG
GACTCGAATTACTAGACCAA

Product: PAS/PAC sensor-containing diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MKKNKERTLRLMIIDDNPENTEAIVTLLRNSGIAVRPSRPQHLEDVTMMLSHPIDLVLVGNSSQISLRALHQQISTSGKD
VPLIQLTWQLNEKEWIAATGFGIRSIALGHHPKHLLEVVNNEIEALQIRRSLRRFEAQIRETERRCNALIASSRDPIAYV
HEGMHIQANQAYLEMFGFESFEDIEGLPLLDLIAAPHMEGFKHLLKTLSKGEKPPSQYKLDARHVNGNIFPATMEFTTTT
YEGEPCIQVVLRGYEEFDQELTREIEKLRQRDPITGLLNRQAFIIALEDAVARAGMNGDQFGLLLVEPNHYTRILQEIGL
DSADTLITSLANFFSEMIDSKVAVRTARFCETRFALLLEGDYTHTITLAERIRIDIAQHIFLLGKHSTTVTVSIGGVQIG
ERIANLCKVLHHAAESVQIATQLGGNTAIIYDPAAADRAEELRIQNLLKQLQQAVTNNDLTIYYQPIMSLQQQSLELYQI
YIGLHGDEELISSVTLLEIAKKRELIAQIDRWVVARAIAQIGERQRAGHTTHLLVQIDSASFTDPQLLTMLRTELAAHHV
RGEQLWLQTPESKVFTHLYNAQQFLSEISVQGCKMGLEQFGTGLDSFQLLTHFKPAFLKLDRNLTQDINSNHDSLEKIRE
ITSRAQDNGIATFAEFVTDSTSMSALFKVGVDYVQGDFVAPISATMNNEF

Sequences:

>Translated_690_residues
MKKNKERTLRLMIIDDNPENTEAIVTLLRNSGIAVRPSRPQHLEDVTMMLSHPIDLVLVGNSSQISLRALHQQISTSGKD
VPLIQLTWQLNEKEWIAATGFGIRSIALGHHPKHLLEVVNNEIEALQIRRSLRRFEAQIRETERRCNALIASSRDPIAYV
HEGMHIQANQAYLEMFGFESFEDIEGLPLLDLIAAPHMEGFKHLLKTLSKGEKPPSQYKLDARHVNGNIFPATMEFTTTT
YEGEPCIQVVLRGYEEFDQELTREIEKLRQRDPITGLLNRQAFIIALEDAVARAGMNGDQFGLLLVEPNHYTRILQEIGL
DSADTLITSLANFFSEMIDSKVAVRTARFCETRFALLLEGDYTHTITLAERIRIDIAQHIFLLGKHSTTVTVSIGGVQIG
ERIANLCKVLHHAAESVQIATQLGGNTAIIYDPAAADRAEELRIQNLLKQLQQAVTNNDLTIYYQPIMSLQQQSLELYQI
YIGLHGDEELISSVTLLEIAKKRELIAQIDRWVVARAIAQIGERQRAGHTTHLLVQIDSASFTDPQLLTMLRTELAAHHV
RGEQLWLQTPESKVFTHLYNAQQFLSEISVQGCKMGLEQFGTGLDSFQLLTHFKPAFLKLDRNLTQDINSNHDSLEKIRE
ITSRAQDNGIATFAEFVTDSTSMSALFKVGVDYVQGDFVAPISATMNNEF
>Mature_690_residues
MKKNKERTLRLMIIDDNPENTEAIVTLLRNSGIAVRPSRPQHLEDVTMMLSHPIDLVLVGNSSQISLRALHQQISTSGKD
VPLIQLTWQLNEKEWIAATGFGIRSIALGHHPKHLLEVVNNEIEALQIRRSLRRFEAQIRETERRCNALIASSRDPIAYV
HEGMHIQANQAYLEMFGFESFEDIEGLPLLDLIAAPHMEGFKHLLKTLSKGEKPPSQYKLDARHVNGNIFPATMEFTTTT
YEGEPCIQVVLRGYEEFDQELTREIEKLRQRDPITGLLNRQAFIIALEDAVARAGMNGDQFGLLLVEPNHYTRILQEIGL
DSADTLITSLANFFSEMIDSKVAVRTARFCETRFALLLEGDYTHTITLAERIRIDIAQHIFLLGKHSTTVTVSIGGVQIG
ERIANLCKVLHHAAESVQIATQLGGNTAIIYDPAAADRAEELRIQNLLKQLQQAVTNNDLTIYYQPIMSLQQQSLELYQI
YIGLHGDEELISSVTLLEIAKKRELIAQIDRWVVARAIAQIGERQRAGHTTHLLVQIDSASFTDPQLLTMLRTELAAHHV
RGEQLWLQTPESKVFTHLYNAQQFLSEISVQGCKMGLEQFGTGLDSFQLLTHFKPAFLKLDRNLTQDINSNHDSLEKIRE
ITSRAQDNGIATFAEFVTDSTSMSALFKVGVDYVQGDFVAPISATMNNEF

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=442, Percent_Identity=26.4705882352941, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788849, Length=245, Percent_Identity=24.8979591836735, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787541, Length=432, Percent_Identity=23.1481481481481, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790496, Length=240, Percent_Identity=21.6666666666667, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082096, Length=242, Percent_Identity=21.4876033057851, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 77712; Mature: 77712

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKERTLRLMIIDDNPENTEAIVTLLRNSGIAVRPSRPQHLEDVTMMLSHPIDLVLVG
CCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCEEEEEEC
NSSQISLRALHQQISTSGKDVPLIQLTWQLNEKEWIAATGFGIRSIALGHHPKHLLEVVN
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCHHHHHHHHH
NEIEALQIRRSLRRFEAQIRETERRCNALIASSRDPIAYVHEGMHIQANQAYLEMFGFES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCHHHHHHHCCCC
FEDIEGLPLLDLIAAPHMEGFKHLLKTLSKGEKPPSQYKLDARHVNGNIFPATMEFTTTT
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCEEEEEECCCCEEEEEEEEEEEE
YEGEPCIQVVLRGYEEFDQELTREIEKLRQRDPITGLLNRQAFIIALEDAVARAGMNGDQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEHHHHHHCCCCCCC
FGLLLVEPNHYTRILQEIGLDSADTLITSLANFFSEMIDSKVAVRTARFCETRFALLLEG
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEC
DYTHTITLAERIRIDIAQHIFLLGKHSTTVTVSIGGVQIGERIANLCKVLHHAAESVQIA
CCEEEEEHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
TQLGGNTAIIYDPAAADRAEELRIQNLLKQLQQAVTNNDLTIYYQPIMSLQQQSLELYQI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHEEEEE
YIGLHGDEELISSVTLLEIAKKRELIAQIDRWVVARAIAQIGERQRAGHTTHLLVQIDSA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
SFTDPQLLTMLRTELAAHHVRGEQLWLQTPESKVFTHLYNAQQFLSEISVQGCKMGLEQF
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
GTGLDSFQLLTHFKPAFLKLDRNLTQDINSNHDSLEKIREITSRAQDNGIATFAEFVTDS
CCCCHHHHHHHHCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TSMSALFKVGVDYVQGDFVAPISATMNNEF
HHHHHHHHHHHHHHCCCEECCHHHHCCCCC
>Mature Secondary Structure
MKKNKERTLRLMIIDDNPENTEAIVTLLRNSGIAVRPSRPQHLEDVTMMLSHPIDLVLVG
CCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCEEEEEEC
NSSQISLRALHQQISTSGKDVPLIQLTWQLNEKEWIAATGFGIRSIALGHHPKHLLEVVN
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCHHHHHHHHH
NEIEALQIRRSLRRFEAQIRETERRCNALIASSRDPIAYVHEGMHIQANQAYLEMFGFES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCHHHHHHHCCCC
FEDIEGLPLLDLIAAPHMEGFKHLLKTLSKGEKPPSQYKLDARHVNGNIFPATMEFTTTT
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCHHCEEEEEECCCCEEEEEEEEEEEE
YEGEPCIQVVLRGYEEFDQELTREIEKLRQRDPITGLLNRQAFIIALEDAVARAGMNGDQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEHHHHHHCCCCCCC
FGLLLVEPNHYTRILQEIGLDSADTLITSLANFFSEMIDSKVAVRTARFCETRFALLLEG
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEC
DYTHTITLAERIRIDIAQHIFLLGKHSTTVTVSIGGVQIGERIANLCKVLHHAAESVQIA
CCEEEEEHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
TQLGGNTAIIYDPAAADRAEELRIQNLLKQLQQAVTNNDLTIYYQPIMSLQQQSLELYQI
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHEEEEE
YIGLHGDEELISSVTLLEIAKKRELIAQIDRWVVARAIAQIGERQRAGHTTHLLVQIDSA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
SFTDPQLLTMLRTELAAHHVRGEQLWLQTPESKVFTHLYNAQQFLSEISVQGCKMGLEQF
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
GTGLDSFQLLTHFKPAFLKLDRNLTQDINSNHDSLEKIREITSRAQDNGIATFAEFVTDS
CCCCHHHHHHHHCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TSMSALFKVGVDYVQGDFVAPISATMNNEF
HHHHHHHHHHHHHHCCCEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]