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The map label for this gene is tolC [H]

Identifier: 182682581

GI number: 182682581

Start: 2324197

End: 2325555

Strand: Direct

Name: tolC [H]

Synonym: XfasM23_2069

Alternate gene names: 182682581

Gene position: 2324197-2325555 (Clockwise)

Preceding gene: 182682580

Following gene: 182682588

Centisome position: 91.66

GC content: 53.64

Gene sequence:

>1359_bases
ATGATCCGCCGCTGCCTCGCCTTGGCCGTGACCACCGCCCTATTTCCCGTCGTTTCACAAGCGACAAACCTATTACAAGT
CTACGAAATGGCACGCGCTGCTGACCCTCAATTAAGCGCAGCCGAATCCACTCGGCTATACAGCGTTGAAGGGCAAGTTC
AGGCACGTGCAGCGCTGCTGCCGCAGATGAGCGGCACAGCCACCCTGGCACGTAGCCGCACTGAATACGACGGTATCAGC
GGATCAGCCATCAGTAAGAACCGCCAATACAGCATCAACGGTAGCCAAACTCTGTTCAACTGGAGCCAGTTTTCCAACCT
TCGATCACAACGCGAGATCGCCAAAGCCGCCGATTTCACACTGGAATCGGCCAAACAAAACTTGATTGTGCGCAGCTCAG
CTGCATACTTCAGCGTCTTGATCGGGATTGAATCGCTGATCGCCGCCGAAGCGAATGAAGCATCAGCTAAGCGCCAGTTT
GATTACGCACAGAAACGTCTTGAGGTTGGCCTATCACCCATCACAGACCTTCATGAAGCACGTGCCAGCTACGACCAAGC
CCGTGCAGACACCATCACTGCACGCAACACACTGCAAGACTACTACCAAGCATTGGCAGAGATCACTGGACAACCCGTCA
CTGACCTGCGTGGCCTCCCTGAAGACTTCCGACCAGAAGTGCCAGCGAAATTCAGTAACATTGACCAGCTGGTCACTGAA
GCCATCTCCAACAATCCGTCGCTAAAAGCGCAGCAATTGCAAGTCAATGCCGCGGAATCCAGCATCTCAGCCGCACGTGG
CGAGCACTACCCAACACTGTCACTGAGTGGAAGCCTGGGGAAAAGTAAAGCCTGGGGATCCTCCAGCGGCGCAATGAGCG
CACTCATTCCTGACAGGAACCAACCGATTAGCGACACCAACAACTTACAATTAACGCTGTCAATACCACTCTTTGCAGGT
GGTGCAACCCAATCCAGGGTCCGTCAGGCAATCGCACAACGCGACATCCAACAGGATAACTACGAGCAGAATAAGCGCAC
GCTAAACCGTAATACCCGCAATGCCTACCAAAATATGATTGCAGGCATCAGCGAGGTCGAAGCGCGTCGCTTGGCAGTCG
TCTCAGCACAAGCGGCCCTAGATGCTTCTCAAGTCGGCCTGGAAGTCGGCACCCGAACCCTGCTGGACGTGGTACAGAAC
CAACGTATCCTTTTCTCGGTACGGCAAGCCTATGTACAAGCACGCTACAACTTCCTACAAAACCGTCTACTACTTTCCCA
GAACAGCGGCACCCTGAGCATTGAGGATGTACAAGAGATCAATCAATTGCTGACGGCTGATTCCGAGAGAAAACTGTAA

Upstream 100 bases:

>100_bases
CCAAACATCACATCCCTGTTTGAGACCGATCTGGCTTACCTCAAAGGTGGCGAGCCAGCCCTCCAATTTCATTTTTGATA
CCTGACTCAAGGATAACGCT

Downstream 100 bases:

>100_bases
GGAGAAGTAAGGAGAAGCATACGATCAGGTGTGAGGACGCACGAACCAATGCCATTACAGGAACGTGGTCCGTGTTTTCT
GCTGCTTTACACACGATCGT

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MIRRCLALAVTTALFPVVSQATNLLQVYEMARAADPQLSAAESTRLYSVEGQVQARAALLPQMSGTATLARSRTEYDGIS
GSAISKNRQYSINGSQTLFNWSQFSNLRSQREIAKAADFTLESAKQNLIVRSSAAYFSVLIGIESLIAAEANEASAKRQF
DYAQKRLEVGLSPITDLHEARASYDQARADTITARNTLQDYYQALAEITGQPVTDLRGLPEDFRPEVPAKFSNIDQLVTE
AISNNPSLKAQQLQVNAAESSISAARGEHYPTLSLSGSLGKSKAWGSSSGAMSALIPDRNQPISDTNNLQLTLSIPLFAG
GATQSRVRQAIAQRDIQQDNYEQNKRTLNRNTRNAYQNMIAGISEVEARRLAVVSAQAALDASQVGLEVGTRTLLDVVQN
QRILFSVRQAYVQARYNFLQNRLLLSQNSGTLSIEDVQEINQLLTADSERKL

Sequences:

>Translated_452_residues
MIRRCLALAVTTALFPVVSQATNLLQVYEMARAADPQLSAAESTRLYSVEGQVQARAALLPQMSGTATLARSRTEYDGIS
GSAISKNRQYSINGSQTLFNWSQFSNLRSQREIAKAADFTLESAKQNLIVRSSAAYFSVLIGIESLIAAEANEASAKRQF
DYAQKRLEVGLSPITDLHEARASYDQARADTITARNTLQDYYQALAEITGQPVTDLRGLPEDFRPEVPAKFSNIDQLVTE
AISNNPSLKAQQLQVNAAESSISAARGEHYPTLSLSGSLGKSKAWGSSSGAMSALIPDRNQPISDTNNLQLTLSIPLFAG
GATQSRVRQAIAQRDIQQDNYEQNKRTLNRNTRNAYQNMIAGISEVEARRLAVVSAQAALDASQVGLEVGTRTLLDVVQN
QRILFSVRQAYVQARYNFLQNRLLLSQNSGTLSIEDVQEINQLLTADSERKL
>Mature_452_residues
MIRRCLALAVTTALFPVVSQATNLLQVYEMARAADPQLSAAESTRLYSVEGQVQARAALLPQMSGTATLARSRTEYDGIS
GSAISKNRQYSINGSQTLFNWSQFSNLRSQREIAKAADFTLESAKQNLIVRSSAAYFSVLIGIESLIAAEANEASAKRQF
DYAQKRLEVGLSPITDLHEARASYDQARADTITARNTLQDYYQALAEITGQPVTDLRGLPEDFRPEVPAKFSNIDQLVTE
AISNNPSLKAQQLQVNAAESSISAARGEHYPTLSLSGSLGKSKAWGSSSGAMSALIPDRNQPISDTNNLQLTLSIPLFAG
GATQSRVRQAIAQRDIQQDNYEQNKRTLNRNTRNAYQNMIAGISEVEARRLAVVSAQAALDASQVGLEVGTRTLLDVVQN
QRILFSVRQAYVQARYNFLQNRLLLSQNSGTLSIEDVQEINQLLTADSERKL

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=432, Percent_Identity=34.4907407407407, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49497; Mature: 49497

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRCLALAVTTALFPVVSQATNLLQVYEMARAADPQLSAAESTRLYSVEGQVQARAALL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHC
PQMSGTATLARSRTEYDGISGSAISKNRQYSINGSQTLFNWSQFSNLRSQREIAKAADFT
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHH
LESAKQNLIVRSSAAYFSVLIGIESLIAAEANEASAKRQFDYAQKRLEVGLSPITDLHEA
HHHHHHCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RASYDQARADTITARNTLQDYYQALAEITGQPVTDLRGLPEDFRPEVPAKFSNIDQLVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCCCHHHHHHHHHHHH
AISNNPSLKAQQLQVNAAESSISAARGEHYPTLSLSGSLGKSKAWGSSSGAMSALIPDRN
HHCCCCCCCHHHEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCC
QPISDTNNLQLTLSIPLFAGGATQSRVRQAIAQRDIQQDNYEQNKRTLNRNTRNAYQNMI
CCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
AGISEVEARRLAVVSAQAALDASQVGLEVGTRTLLDVVQNQRILFSVRQAYVQARYNFLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NRLLLSQNSGTLSIEDVQEINQLLTADSERKL
HHHEEECCCCCEEHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIRRCLALAVTTALFPVVSQATNLLQVYEMARAADPQLSAAESTRLYSVEGQVQARAALL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHC
PQMSGTATLARSRTEYDGISGSAISKNRQYSINGSQTLFNWSQFSNLRSQREIAKAADFT
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEECHHHHHHHHHHHHHHHHHHHH
LESAKQNLIVRSSAAYFSVLIGIESLIAAEANEASAKRQFDYAQKRLEVGLSPITDLHEA
HHHHHHCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RASYDQARADTITARNTLQDYYQALAEITGQPVTDLRGLPEDFRPEVPAKFSNIDQLVTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHCCCCCHHHHHHHHHHHH
AISNNPSLKAQQLQVNAAESSISAARGEHYPTLSLSGSLGKSKAWGSSSGAMSALIPDRN
HHCCCCCCCHHHEEEEHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCC
QPISDTNNLQLTLSIPLFAGGATQSRVRQAIAQRDIQQDNYEQNKRTLNRNTRNAYQNMI
CCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
AGISEVEARRLAVVSAQAALDASQVGLEVGTRTLLDVVQNQRILFSVRQAYVQARYNFLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NRLLLSQNSGTLSIEDVQEINQLLTADSERKL
HHHEEECCCCCEEHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8801424 [H]